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The map label for this gene is pyrI

Identifier: 209694111

GI number: 209694111

Start: 572628

End: 573092

Strand: Reverse

Name: pyrI

Synonym: VSAL_I0518

Alternate gene names: 209694111

Gene position: 573092-572628 (Counterclockwise)

Preceding gene: 209694112

Following gene: 209694110

Centisome position: 17.23

GC content: 38.28

Gene sequence:

>465_bases
ATGTCGAATAAAACTGAATTACGTGTTGAAGCAATCAAAAACGGCACCGTTATCGATCACATTCCTGCAAATATTGGTGT
AAAAGTGCTTAAATTGTTCCAAATGGACAAAACAGCAGAGCGTGTGACCATTGGCTTAAACTTACCGTCTTCAGCGCTTG
GCTCAAAAGATTTACTGAAAATAGAAAATACATTTGTCACACCAGAACAAGCGAGTAAATTGGCGTTGTATGCACCACAC
GCAACCGTTAATCAAATTGAGAACTATGAAGTCGTTAAAAAGATACCGCTAATTCTTCCTAAGCAAATCACAGGGGTATT
TGAGTGTCCGAACAGCAATTGCATTACTCATGGTGAGCCAGTAGATAGCAGCTTTAAAGTGATAGCTAAGAAAGGAAACA
TCCATCTGAAGTGCAAATATTGTGAAAAAGTATACTCTCAAGAAGTCGTGACCGATTTACATTAA

Upstream 100 bases:

>100_bases
CCACACGCGTATTATTTCCAACAAGCTGAAAATGGGGTTTATGCTCGCCAAGCATTACTTGCTCTTGTTTTGAATGAATC
ACTATAAGGAAGAAAATCAA

Downstream 100 bases:

>100_bases
GCAGACGACAACGAAAGTGAATTAAATTGTCTTCATCTTCTCAGCCTCGATATTCTCGGGGCTGTTTTCTTATCCGCATT
TGTTTTATATTGAGTTTATC

Product: aspartate carbamoyltransferase regulatory subunit

Products: phosphate; N-carbamoyl-L-aspartate [C]

Alternate protein names: NA

Number of amino acids: Translated: 154; Mature: 153

Protein sequence:

>154_residues
MSNKTELRVEAIKNGTVIDHIPANIGVKVLKLFQMDKTAERVTIGLNLPSSALGSKDLLKIENTFVTPEQASKLALYAPH
ATVNQIENYEVVKKIPLILPKQITGVFECPNSNCITHGEPVDSSFKVIAKKGNIHLKCKYCEKVYSQEVVTDLH

Sequences:

>Translated_154_residues
MSNKTELRVEAIKNGTVIDHIPANIGVKVLKLFQMDKTAERVTIGLNLPSSALGSKDLLKIENTFVTPEQASKLALYAPH
ATVNQIENYEVVKKIPLILPKQITGVFECPNSNCITHGEPVDSSFKVIAKKGNIHLKCKYCEKVYSQEVVTDLH
>Mature_153_residues
SNKTELRVEAIKNGTVIDHIPANIGVKVLKLFQMDKTAERVTIGLNLPSSALGSKDLLKIENTFVTPEQASKLALYAPHA
TVNQIENYEVVKKIPLILPKQITGVFECPNSNCITHGEPVDSSFKVIAKKGNIHLKCKYCEKVYSQEVVTDLH

Specific function: Involved in allosteric regulation of aspartate carbamoyltransferase

COG id: COG1781

COG function: function code F; Aspartate carbamoyltransferase, regulatory subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyrI family

Homologues:

Organism=Escherichia coli, GI1790692, Length=150, Percent_Identity=60.6666666666667, Blast_Score=191, Evalue=1e-50,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 440 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): PYRI_ALISL (B6EMC2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002262039.1
- GeneID:   6986536
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0518
- HOGENOM:   HBG305327
- OMA:   EGTVIDH
- ProtClustDB:   PRK00893
- HAMAP:   MF_00002
- InterPro:   IPR020545
- InterPro:   IPR002801
- InterPro:   IPR020542
- Gene3D:   G3DSA:2.30.30.20
- Gene3D:   G3DSA:3.30.70.140
- ProDom:   PD006194
- TIGRFAMs:   TIGR00240

Pfam domain/function: PF01948 PyrI; PF02748 PyrI_C; SSF54893 Asp_carbamoylTrfase_reg; SSF57825 Asp_transf_reg_C

EC number: NA

Molecular weight: Translated: 17042; Mature: 16911

Theoretical pI: Translated: 8.62; Mature: 8.62

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNKTELRVEAIKNGTVIDHIPANIGVKVLKLFQMDKTAERVTIGLNLPSSALGSKDLLK
CCCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCCCCEEE
IENTFVTPEQASKLALYAPHATVNQIENYEVVKKIPLILPKQITGVFECPNSNCITHGEP
ECCEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCEECCCC
VDSSFKVIAKKGNIHLKCKYCEKVYSQEVVTDLH
CCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SNKTELRVEAIKNGTVIDHIPANIGVKVLKLFQMDKTAERVTIGLNLPSSALGSKDLLK
CCCCEEEEEEECCCEEEEECCCCCCHHHHHHHHCCCCCCEEEEEECCCCHHCCCCCEEE
IENTFVTPEQASKLALYAPHATVNQIENYEVVKKIPLILPKQITGVFECPNSNCITHGEP
ECCEEECCCCCCEEEEECCCCHHHHHCCHHHHHHCCCCCCCCCCEEEECCCCCCEECCCC
VDSSFKVIAKKGNIHLKCKYCEKVYSQEVVTDLH
CCCCEEEEEECCCEEEEEHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Zn [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: carbamoyl phosphate; L-aspartate [C]

Specific reaction: carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate [C]

General reaction: Transferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA