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The map label for this gene is cysN

Identifier: 209694022

GI number: 209694022

Start: 457371

End: 458807

Strand: Direct

Name: cysN

Synonym: VSAL_I0421

Alternate gene names: 209694022

Gene position: 457371-458807 (Clockwise)

Preceding gene: 209694021

Following gene: 209694023

Centisome position: 13.75

GC content: 40.5

Gene sequence:

>1437_bases
ATGAACAGTGCAGTAGAACAAGAACTTGCAGAGTTAGGTATCGAAGCGTATTTAAGCCAACATCAACATAAATCAATGCT
GCGTTTTTTAACGTGTGGTTCGGTAGATGATGGAAAAAGTACTCTTATAGGCCGATTACTTCATGACTCAAAACAAATTT
ATGCGGATCAACTGGAAGCCGTGCATGCTGACAGCCAACGCGTAGGAACGACTGGCGAACGCCCTGACTTAGCATTACTT
GTTGATGGCTTACAAGCAGAGCGTGAGCAAGGCATTACGATTGATGTCGCTTACCGTTATTTCTCAACTCAAAAGCGTAA
ATTCATTATTTCTGATACACCAGGACATGAGCAATATACACGTAATATGGCAACGGGTGCATCAACGTGTGATGTCGCTG
TTATCTTAATCGATGCTCGTAAAGGTGTGTTAGATCAAACTCGTCGCCATTCCTATATCGCGAATCTCTTAGGTATTCGT
CATTTTGTGGTGGCGGTGAATAAAATGGATTTGGTTGATTATTCTCAATCTCGATTTGAAGAGATTAAAGAAGAATATGT
AACGTTTTCTAAAAAACTTAATAATCCTAATTTGGATATTTCGATTCTGCCGTTATCAGCATTAGAAGGCGATAACGTAG
TAAATCACAGTGCCGCACTTGCTTGGTATAAAGGTGCCCCATTATTAGAAGTGTTAGAAGACATTGATATTGATGCTGAT
AGAGGGAATGGTGAATTTCGTTTCCCTGTGCAATATGTTAATCGCCCTAATCTTGATTTCCGAGGTTTCTCGGGTTCTGT
GTCTTCTGGTGAGATAAAGGTTGGTGATGACATTATTGCTTTGCCGTCAGGTAAAAAATCAAAAGTAGCTCGTATTGTGA
CGTTTGATGGTGATTTACCTTCGGCGCAAGCGGGTCAGGCCGTGACTCTCACACTAGAAGATGAAATTGATATTAGTCGT
GGTGATCTATTGGTTAAATCGACATCCAATCTTACTGCAACGGATCAATTTAAAGCCGAAATTGTTTGGATGACAGAAAA
AGGGTTACAGCCTGGACGTCAATATGACATTAAAATAGCAGGCAAAAAAACGGTCGGTCAAATTGATGCTATCCATCATC
AAGTCAATATTAATAGCCTTGAGACGTTTGTTACCGATGAGTTACCTCTTAATGGTATCGGGTTATGTGATGTTTCATTA
ACTGAGGTGGTGAGCTTAGATCGTTATCAAGACTGTGCTGACACTGGTGGGTTTATCTTTATTGACCGTTTAACGAATGT
GACAGTAGGGGCAGGCATGATCCAAAACCTATCTGAGTTGAGTGATGTCGCTCCTATCAATGATAATGTCAGTGCTTTTG
AAGTGGAATTAAATGCGTTAGTTCGTAAGCATTTTCCACATTGGGGCGCGCAAGATATTTCGAAGCTCTTAAGTTAG

Upstream 100 bases:

>100_bases
CGACAAGGGCGAGCCATTGATCATGACTCATCTGGATCGATGGAACAGAAAAAACGCCAGGGTTATTTCTAAATATTAAA
TCAGGACAAGGAAGATAAAT

Downstream 100 bases:

>100_bases
AGTTAAGAGTAAGGTAATTACTATGCCGTGGGAACAAGGATTCGTTCTAGCTTTATTAACTCTTATTATTGTGAGTTTAT
TGGCGAGTAAATTAAAACCA

Product: sulfate adenylyltransferase subunit 1

Products: NA

Alternate protein names: ATP-sulfurylase large subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MNSAVEQELAELGIEAYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLEAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIISDTPGHEQYTRNMATGASTCDVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVDYSQSRFEEIKEEYVTFSKKLNNPNLDISILPLSALEGDNVVNHSAALAWYKGAPLLEVLEDIDIDAD
RGNGEFRFPVQYVNRPNLDFRGFSGSVSSGEIKVGDDIIALPSGKKSKVARIVTFDGDLPSAQAGQAVTLTLEDEIDISR
GDLLVKSTSNLTATDQFKAEIVWMTEKGLQPGRQYDIKIAGKKTVGQIDAIHHQVNINSLETFVTDELPLNGIGLCDVSL
TEVVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSELSDVAPINDNVSAFEVELNALVRKHFPHWGAQDISKLLS

Sequences:

>Translated_478_residues
MNSAVEQELAELGIEAYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLEAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIISDTPGHEQYTRNMATGASTCDVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVDYSQSRFEEIKEEYVTFSKKLNNPNLDISILPLSALEGDNVVNHSAALAWYKGAPLLEVLEDIDIDAD
RGNGEFRFPVQYVNRPNLDFRGFSGSVSSGEIKVGDDIIALPSGKKSKVARIVTFDGDLPSAQAGQAVTLTLEDEIDISR
GDLLVKSTSNLTATDQFKAEIVWMTEKGLQPGRQYDIKIAGKKTVGQIDAIHHQVNINSLETFVTDELPLNGIGLCDVSL
TEVVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSELSDVAPINDNVSAFEVELNALVRKHFPHWGAQDISKLLS
>Mature_478_residues
MNSAVEQELAELGIEAYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLEAVHADSQRVGTTGERPDLALL
VDGLQAEREQGITIDVAYRYFSTQKRKFIISDTPGHEQYTRNMATGASTCDVAVILIDARKGVLDQTRRHSYIANLLGIR
HFVVAVNKMDLVDYSQSRFEEIKEEYVTFSKKLNNPNLDISILPLSALEGDNVVNHSAALAWYKGAPLLEVLEDIDIDAD
RGNGEFRFPVQYVNRPNLDFRGFSGSVSSGEIKVGDDIIALPSGKKSKVARIVTFDGDLPSAQAGQAVTLTLEDEIDISR
GDLLVKSTSNLTATDQFKAEIVWMTEKGLQPGRQYDIKIAGKKTVGQIDAIHHQVNINSLETFVTDELPLNGIGLCDVSL
TEVVSLDRYQDCADTGGFIFIDRLTNVTVGAGMIQNLSELSDVAPINDNVSAFEVELNALVRKHFPHWGAQDISKLLS

Specific function: May be the GTPase, regulating ATP sulfurylase activity

COG id: COG2895

COG function: function code P; GTPases - Sulfate adenylate transferase subunit 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. CysN/nodQ subfamily

Homologues:

Organism=Homo sapiens, GI223555963, Length=452, Percent_Identity=28.7610619469027, Blast_Score=171, Evalue=1e-42,
Organism=Homo sapiens, GI5729864, Length=452, Percent_Identity=28.7610619469027, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI194018520, Length=415, Percent_Identity=29.1566265060241, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194018522, Length=415, Percent_Identity=29.1566265060241, Blast_Score=162, Evalue=5e-40,
Organism=Homo sapiens, GI194097354, Length=415, Percent_Identity=29.1566265060241, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI46094014, Length=415, Percent_Identity=28.433734939759, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI4503471, Length=276, Percent_Identity=33.695652173913, Blast_Score=145, Evalue=6e-35,
Organism=Homo sapiens, GI4503475, Length=351, Percent_Identity=28.7749287749288, Blast_Score=142, Evalue=6e-34,
Organism=Homo sapiens, GI34147630, Length=329, Percent_Identity=29.7872340425532, Blast_Score=94, Evalue=3e-19,
Organism=Escherichia coli, GI1789108, Length=474, Percent_Identity=63.9240506329114, Blast_Score=625, Evalue=1e-180,
Organism=Escherichia coli, GI1789737, Length=329, Percent_Identity=27.355623100304, Blast_Score=86, Evalue=8e-18,
Organism=Escherichia coli, GI1790412, Length=329, Percent_Identity=27.355623100304, Blast_Score=85, Evalue=8e-18,
Organism=Escherichia coli, GI2367247, Length=251, Percent_Identity=26.6932270916335, Blast_Score=73, Evalue=4e-14,
Organism=Caenorhabditis elegans, GI17552884, Length=444, Percent_Identity=27.9279279279279, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17569207, Length=444, Percent_Identity=27.9279279279279, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI32566629, Length=342, Percent_Identity=30.7017543859649, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI115532065, Length=333, Percent_Identity=29.1291291291291, Blast_Score=149, Evalue=4e-36,
Organism=Caenorhabditis elegans, GI115532067, Length=333, Percent_Identity=29.1291291291291, Blast_Score=148, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI32566303, Length=437, Percent_Identity=26.7734553775744, Blast_Score=124, Evalue=1e-28,
Organism=Caenorhabditis elegans, GI32566301, Length=149, Percent_Identity=34.8993288590604, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17556456, Length=344, Percent_Identity=27.6162790697674, Blast_Score=85, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI25141371, Length=284, Percent_Identity=25.3521126760563, Blast_Score=82, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325337, Length=324, Percent_Identity=30.8641975308642, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6319594, Length=324, Percent_Identity=30.8641975308642, Blast_Score=153, Evalue=7e-38,
Organism=Saccharomyces cerevisiae, GI6322937, Length=319, Percent_Identity=30.4075235109718, Blast_Score=147, Evalue=3e-36,
Organism=Saccharomyces cerevisiae, GI6320377, Length=434, Percent_Identity=28.110599078341, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6324761, Length=282, Percent_Identity=29.4326241134752, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI45550900, Length=443, Percent_Identity=26.1851015801354, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI17137380, Length=425, Percent_Identity=25.6470588235294, Blast_Score=153, Evalue=3e-37,
Organism=Drosophila melanogaster, GI24652838, Length=351, Percent_Identity=29.3447293447293, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI17137572, Length=351, Percent_Identity=29.3447293447293, Blast_Score=148, Evalue=1e-35,
Organism=Drosophila melanogaster, GI45553807, Length=213, Percent_Identity=36.150234741784, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI45553816, Length=213, Percent_Identity=36.150234741784, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI24651721, Length=213, Percent_Identity=36.150234741784, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI17864154, Length=213, Percent_Identity=36.150234741784, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI281363316, Length=332, Percent_Identity=28.0120481927711, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17864358, Length=332, Percent_Identity=28.0120481927711, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI19921738, Length=270, Percent_Identity=30.7407407407407, Blast_Score=86, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSN_ALISL (B6ELP3)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261950.1
- GeneID:   6988373
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0421
- HOGENOM:   HBG307581
- OMA:   SAQIYED
- ProtClustDB:   PRK05124
- HAMAP:   MF_00062
- InterPro:   IPR000795
- InterPro:   IPR011779
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR009000
- PRINTS:   PR00315
- TIGRFAMs:   TIGR02034

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; SSF50465 Elong_init_C; SSF50447 Translat_factor

EC number: =2.7.7.4

Molecular weight: Translated: 52639; Mature: 52639

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSAVEQELAELGIEAYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHADSQRVGTTGERPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIISDTPGHEQYT
HHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCHHHHH
RNMATGASTCDVAVILIDARKGVLDQTRRHSYIANLLGIRHFVVAVNKMDLVDYSQSRFE
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCHHHHH
EIKEEYVTFSKKLNNPNLDISILPLSALEGDNVVNHSAALAWYKGAPLLEVLEDIDIDAD
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCC
RGNGEFRFPVQYVNRPNLDFRGFSGSVSSGEIKVGDDIIALPSGKKSKVARIVTFDGDLP
CCCCCEEEEHHCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCEEEEEEECCCCC
SAQAGQAVTLTLEDEIDISRGDLLVKSTSNLTATDQFKAEIVWMTEKGLQPGRQYDIKIA
CCCCCCEEEEEEECCCCCCCCCEEEEECCCCEECCCCEEEEEEEECCCCCCCCEEEEEEE
GKKTVGQIDAIHHQVNINSLETFVTDELPLNGIGLCDVSLTEVVSLDRYQDCADTGGFIF
CCCCHHHHHHHHEEECCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEE
IDRLTNVTVGAGMIQNLSELSDVAPINDNVSAFEVELNALVRKHFPHWGAQDISKLLS
EEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHC
>Mature Secondary Structure
MNSAVEQELAELGIEAYLSQHQHKSMLRFLTCGSVDDGKSTLIGRLLHDSKQIYADQLEA
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
VHADSQRVGTTGERPDLALLVDGLQAEREQGITIDVAYRYFSTQKRKFIISDTPGHEQYT
HHCCCCCCCCCCCCCCEEEEECCHHHHHHCCCEEEEEEEECCCCCCEEEEECCCCHHHHH
RNMATGASTCDVAVILIDARKGVLDQTRRHSYIANLLGIRHFVVAVNKMDLVDYSQSRFE
HHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHEECCHHHHCCCHHHHH
EIKEEYVTFSKKLNNPNLDISILPLSALEGDNVVNHSAALAWYKGAPLLEVLEDIDIDAD
HHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCEEEEEECCCCHHHHHHHCCCCCC
RGNGEFRFPVQYVNRPNLDFRGFSGSVSSGEIKVGDDIIALPSGKKSKVARIVTFDGDLP
CCCCCEEEEHHCCCCCCCCCCCCCCCCCCCEEEECCCEEECCCCCCCCEEEEEEECCCCC
SAQAGQAVTLTLEDEIDISRGDLLVKSTSNLTATDQFKAEIVWMTEKGLQPGRQYDIKIA
CCCCCCEEEEEEECCCCCCCCCEEEEECCCCEECCCCEEEEEEEECCCCCCCCEEEEEEE
GKKTVGQIDAIHHQVNINSLETFVTDELPLNGIGLCDVSLTEVVSLDRYQDCADTGGFIF
CCCCHHHHHHHHEEECCCHHHHHEECCCCCCCCEEECCHHHHHHHHHHHHHHHCCCCEEE
IDRLTNVTVGAGMIQNLSELSDVAPINDNVSAFEVELNALVRKHFPHWGAQDISKLLS
EEECCCCEECHHHHHHHHHHHHCCCCCCCCEEEEHHHHHHHHHHCCCCCHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA