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The map label for this gene is cysD

Identifier: 209694021

GI number: 209694021

Start: 456434

End: 457342

Strand: Direct

Name: cysD

Synonym: VSAL_I0420

Alternate gene names: 209694021

Gene position: 456434-457342 (Clockwise)

Preceding gene: 209694019

Following gene: 209694022

Centisome position: 13.73

GC content: 40.37

Gene sequence:

>909_bases
ATGGACGCAAAACGATTAACCCACCTCCAGCAACTTGAAGCGGAAAGTATTCATATTATCCGTGAAGTCGCCGCAGAGTT
TGATAACCCAGTGATGATGTATTCCATTGGTAAAGATTCTTCGGTTATGTTGCATCTTGCTCGTAAAGCTTTTTATCCAG
GAAAAATTCCCTTTCCGCTTCTTCATGTTGATACGGATTGGAAATTCAAAGAGATGATTGAGTTTCGAGATAAAACCGCT
AAAAAATACGGGTTTGACCTATTAGTACACAAAAACCCTGAAGGGATAGAAATGGGGATTAGCCCATTCGTACATGGTTC
TTCAAAGCACACCGATATCATGAAAACTCAAGGTCTGAAACAAGCGCTAAATCAGCATGGATTTGATGCTGCATTTGGTG
GGGCTCGTCGTGATGAAGAAAAGTCTCGAGCAAAAGAGCGTGTGTATTCTTTCCGCGATAAAAATCATACGTGGGATCCA
AAAAATCAACGTCCTGAATTGTGGAATACCTATAACGGTCAAGTAAATAAAGGTGAAAGTATTCGTGTTTTCCCATTATC
AAACTGGACGGAATTGGATATTTGGCAGTACATTTATTTAGAAAGTATCGATATTGTTCCGCTTTATTTAGCTGAAAAAC
GCCCAGTAGTTGATCGTGATGGTATGTTAATCATGGTTGATGATGAGCGAATGGAGTTAAAAGAAGGGGAAAAAATTGAA
TACAAAGACATCCGCTTTAGAACATTAGGGTGTTACCCATTAACAGGGGCTGTGGAATCCAAAGCGAACACCTTGCCTGA
AATTATTGAAGAAATGCTGGTTGCTACTTCCAGTGAGCGACAAGGGCGAGCCATTGATCATGACTCATCTGGATCGATGG
AACAGAAAAAACGCCAGGGTTATTTCTAA

Upstream 100 bases:

>100_bases
ATGAATTTTTAGAATTTCAGGTAATAAATGAAGACGTCTATAGTGGTTTTATTGGAATGAGAAATCGCCATCCCCAATTT
AGCCTAAAGGACAATATTAC

Downstream 100 bases:

>100_bases
ATATTAAATCAGGACAAGGAAGATAAATATGAACAGTGCAGTAGAACAAGAACTTGCAGAGTTAGGTATCGAAGCGTATT
TAAGCCAACATCAACATAAA

Product: sulfate adenylyltransferase subunit 2

Products: NA

Alternate protein names: ATP-sulfurylase small subunit; Sulfate adenylate transferase; SAT

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MDAKRLTHLQQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTDWKFKEMIEFRDKTA
KKYGFDLLVHKNPEGIEMGISPFVHGSSKHTDIMKTQGLKQALNQHGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWNTYNGQVNKGESIRVFPLSNWTELDIWQYIYLESIDIVPLYLAEKRPVVDRDGMLIMVDDERMELKEGEKIE
YKDIRFRTLGCYPLTGAVESKANTLPEIIEEMLVATSSERQGRAIDHDSSGSMEQKKRQGYF

Sequences:

>Translated_302_residues
MDAKRLTHLQQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTDWKFKEMIEFRDKTA
KKYGFDLLVHKNPEGIEMGISPFVHGSSKHTDIMKTQGLKQALNQHGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWNTYNGQVNKGESIRVFPLSNWTELDIWQYIYLESIDIVPLYLAEKRPVVDRDGMLIMVDDERMELKEGEKIE
YKDIRFRTLGCYPLTGAVESKANTLPEIIEEMLVATSSERQGRAIDHDSSGSMEQKKRQGYF
>Mature_302_residues
MDAKRLTHLQQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLARKAFYPGKIPFPLLHVDTDWKFKEMIEFRDKTA
KKYGFDLLVHKNPEGIEMGISPFVHGSSKHTDIMKTQGLKQALNQHGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDP
KNQRPELWNTYNGQVNKGESIRVFPLSNWTELDIWQYIYLESIDIVPLYLAEKRPVVDRDGMLIMVDDERMELKEGEKIE
YKDIRFRTLGCYPLTGAVESKANTLPEIIEEMLVATSSERQGRAIDHDSSGSMEQKKRQGYF

Specific function: First step in the sulfate activation pathway. This reaction occurs early in the reductive branch of the cysteine biosynthetic pathway. [C]

COG id: COG0175

COG function: function code EH; 3'-phosphoadenosine 5'-phosphosulfate sulfotransferase (PAPS reductase)/FAD synthetase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PAPS reductase family. CysD subfamily

Homologues:

Organism=Escherichia coli, GI1789109, Length=302, Percent_Identity=80.794701986755, Blast_Score=527, Evalue=1e-151,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSD_ALISL (B6ELP2)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261949.1
- GeneID:   6988346
- GenomeReviews:   FM178379_GR
- KEGG:   vsa:VSAL_I0420
- HOGENOM:   HBG758022
- OMA:   GMIRFRT
- ProtClustDB:   PRK05253
- HAMAP:   MF_00064
- InterPro:   IPR002500
- InterPro:   IPR014729
- InterPro:   IPR011784
- Gene3D:   G3DSA:3.40.50.620
- PIRSF:   PIRSF002936
- TIGRFAMs:   TIGR02039

Pfam domain/function: PF01507 PAPS_reduct

EC number: =2.7.7.4

Molecular weight: Translated: 35020; Mature: 35020

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDAKRLTHLQQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLARKAFYPGKIPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTDWKFKEMIEFRDKTAKKYGFDLLVHKNPEGIEMGISPFVHGSSKHTDIMKTQGLK
EEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHH
QALNQHGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDPKNQRPELWNTYNGQVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIYLESIDIVPLYLAEKRPVVDRDGMLIMVDDERMELKEGEKIE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHCCCCEEE
YKDIRFRTLGCYPLTGAVESKANTLPEIIEEMLVATSSERQGRAIDHDSSGSMEQKKRQG
EECEEEEEECCEECCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCC
YF
CC
>Mature Secondary Structure
MDAKRLTHLQQLEAESIHIIREVAAEFDNPVMMYSIGKDSSVMLHLARKAFYPGKIPFPL
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCEE
LHVDTDWKFKEMIEFRDKTAKKYGFDLLVHKNPEGIEMGISPFVHGSSKHTDIMKTQGLK
EEECCCCCHHHHHHHHHHHHHHHCCEEEEECCCCCCEECCCCCCCCCCCCCHHHHHHHHH
QALNQHGFDAAFGGARRDEEKSRAKERVYSFRDKNHTWDPKNQRPELWNTYNGQVNKGES
HHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCE
IRVFPLSNWTELDIWQYIYLESIDIVPLYLAEKRPVVDRDGMLIMVDDERMELKEGEKIE
EEEEECCCCCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCCEEEEECCCCHHHCCCCEEE
YKDIRFRTLGCYPLTGAVESKANTLPEIIEEMLVATSSERQGRAIDHDSSGSMEQKKRQG
EECEEEEEECCEECCCCHHHHHCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHCC
YF
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA