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The map label for this gene is cysJ [H]

Identifier: 209694003

GI number: 209694003

Start: 439340

End: 441181

Strand: Direct

Name: cysJ [H]

Synonym: VSAL_I0402

Alternate gene names: 209694003

Gene position: 439340-441181 (Clockwise)

Preceding gene: 209694002

Following gene: 209694004

Centisome position: 13.21

GC content: 40.28

Gene sequence:

>1842_bases
ATGAATAGGGATGTCGTCATGTTATTAAAGGAACTCTCCGCACTCGCTAGCCCACTTAATGATCAACAAATAGGTCAATT
ACAGCTAGCCGCTTCAGAATTATCTCCACAACAACTTGCTTGGGTAAGTGGTTATCTTTGGGGGGTAAGCCAAACATCTG
GTGCCACACAACCAGCAAACCAAGCCGCCGTGGCGGTATCAAGTGAGCCTGTTAGTAAATTATCAATTATTTATGCCTCA
CAAACCGGTAATGCAAAAGGTGTCGCTGAAGCATTAAAAGCAGAAGCGTCAGCGATTGGTATTCGTGTTGAATTATTTGA
TGCGAGTGATTATAAAGGCAAAAACCTCGCTAAAGAAACGCACGTTATTATTGTTGCATCTACTAATGGTGAGGGAGAAG
CCCCTGATAATGGCATTGAGCTGCATGAATTTTTACAATCGAAAAAAGCACCTAAGCTAGATAAATTACACTATGGTGTG
ATTGGTTTAGGGGATTCAAGCTATGAATTTTTTTGCCAAACCGGGAAAGATTTTGATGGCTTTCTAGCCAAAAAAGGAGC
AACCGCATTTATTGATCGTATTGATCTGGATGTTGATTATGAAGCTTCTGCCGTTGAGTGGCGTAAACAAGCGTTAGAGA
AAGTAAAAGAAACCTTATCAACAGATAATGAGTCTAAAAATGGACAGGTAGTGCAATTACCTGTTGGTCAAGCGGCGCGC
TCTGCTTCGCAATATTCAAAACAAAACCCATACACCGCGACATTATTAGCCAGTCAGAAAATTACGGGTCGAGATTCAGG
TAAAGATGTTCGCCATATTGAAATTGATCTTGAAGATTCAGGATTAACTTATCAGCCAGGTGATGCATTAGGTGTGTGGT
TTGAAAATAGTCTAGAGTTAGTCACCGCTGTGTTAAAACAAGTTGGATTAACTGGAGAAGAACACATTGAAGTCGATGAT
GAATCAATATCTCTTCAAAAAGCCTTAATTGAAAAATATGAAATCACATCAGCAAATCCACAGCAAGTCAGCAAGTTTTC
AGAGCTATCAGGCAGTAAAAAGTTAGAGAAGTTAGTTCAAGACAAAGATAAGTTACGTCAATACGCTGGCAATACTCAAG
TGATTGATCTGTTATCAGAAAAGAAAACCAAATTAACGGCAGAGCAACTAGTGGGTTTACTTCGCCGTCTAACGCCTCGT
TTATATTCTATTGCTTCAAGCCAGAGTGAAGTGGATGAAGAAGTTCACCTGACTGTCGGTGTGGTTGAATATCAACTGGG
GGATGAAACTCGATTCGGTGGTGCCTCTAGCTTTTTATCTCATCGTCTAGAAGAAGGCGCAGAAGTGAACGTGTTTATTG
AACACAATAATAATTTCAAACTTCCTCAAGATGACAACGTGCCAGTCATCATGATTGGTCCTGGTACGGGTATTGCCCCT
TTCCGTGCGTTTGTTCAAGAACGTGACAATCGTGATGCAGAAGGTAAAAACTGGTTATTCTTTGGTGATAGAACTTTTAC
GCAAGATTTCTTATACCAAGTTGAATGGCAAAAATACCTTAAGTCAGGTGTTATAAATCAATTAGATGTGGCGTTCAGTC
GTGATCAACAAGAAAAAGTCTATGTTCAACATCGTATCTTAGAGCATTCAATTCAAGTGTGGCAGTGGCTGCAAGATGGC
GCTTATATTTATGTCTGTGGTGATGCCACTCGCATGGCAAAAGACGTACATGAAGCATTAATTACGATTGTTGAGCAGCA
AGGAAAAAAGAACAGAGAAGAAGCAGAGCAATTTGTAAATGATCTGCGTAAGGCGAAACGTTACCAAAGGGATGTGTACT
AA

Upstream 100 bases:

>100_bases
ATAAAGACAAAGCAAACATTTCTTTCTTCCAAAAAGTTATAAAACATTCTTATCTATTCTTTCTTGTTATTGGAAAGAGT
CGCTAATCTAACATGACGCA

Downstream 100 bases:

>100_bases
TGAGCGAACAAATTGCAGTAAAAACAATCTTATCAGGCACAGAACTTGGCCCATTAGCGGATAACGAGCGTCTAAAGCGT
GAAAGCAAAAACCTTCGAGG

Product: sulfite reductase [NADPH] flavoprotein alpha-component

Products: NA

Alternate protein names: SiR-FP [H]

Number of amino acids: Translated: 613; Mature: 613

Protein sequence:

>613_residues
MNRDVVMLLKELSALASPLNDQQIGQLQLAASELSPQQLAWVSGYLWGVSQTSGATQPANQAAVAVSSEPVSKLSIIYAS
QTGNAKGVAEALKAEASAIGIRVELFDASDYKGKNLAKETHVIIVASTNGEGEAPDNGIELHEFLQSKKAPKLDKLHYGV
IGLGDSSYEFFCQTGKDFDGFLAKKGATAFIDRIDLDVDYEASAVEWRKQALEKVKETLSTDNESKNGQVVQLPVGQAAR
SASQYSKQNPYTATLLASQKITGRDSGKDVRHIEIDLEDSGLTYQPGDALGVWFENSLELVTAVLKQVGLTGEEHIEVDD
ESISLQKALIEKYEITSANPQQVSKFSELSGSKKLEKLVQDKDKLRQYAGNTQVIDLLSEKKTKLTAEQLVGLLRRLTPR
LYSIASSQSEVDEEVHLTVGVVEYQLGDETRFGGASSFLSHRLEEGAEVNVFIEHNNNFKLPQDDNVPVIMIGPGTGIAP
FRAFVQERDNRDAEGKNWLFFGDRTFTQDFLYQVEWQKYLKSGVINQLDVAFSRDQQEKVYVQHRILEHSIQVWQWLQDG
AYIYVCGDATRMAKDVHEALITIVEQQGKKNREEAEQFVNDLRKAKRYQRDVY

Sequences:

>Translated_613_residues
MNRDVVMLLKELSALASPLNDQQIGQLQLAASELSPQQLAWVSGYLWGVSQTSGATQPANQAAVAVSSEPVSKLSIIYAS
QTGNAKGVAEALKAEASAIGIRVELFDASDYKGKNLAKETHVIIVASTNGEGEAPDNGIELHEFLQSKKAPKLDKLHYGV
IGLGDSSYEFFCQTGKDFDGFLAKKGATAFIDRIDLDVDYEASAVEWRKQALEKVKETLSTDNESKNGQVVQLPVGQAAR
SASQYSKQNPYTATLLASQKITGRDSGKDVRHIEIDLEDSGLTYQPGDALGVWFENSLELVTAVLKQVGLTGEEHIEVDD
ESISLQKALIEKYEITSANPQQVSKFSELSGSKKLEKLVQDKDKLRQYAGNTQVIDLLSEKKTKLTAEQLVGLLRRLTPR
LYSIASSQSEVDEEVHLTVGVVEYQLGDETRFGGASSFLSHRLEEGAEVNVFIEHNNNFKLPQDDNVPVIMIGPGTGIAP
FRAFVQERDNRDAEGKNWLFFGDRTFTQDFLYQVEWQKYLKSGVINQLDVAFSRDQQEKVYVQHRILEHSIQVWQWLQDG
AYIYVCGDATRMAKDVHEALITIVEQQGKKNREEAEQFVNDLRKAKRYQRDVY
>Mature_613_residues
MNRDVVMLLKELSALASPLNDQQIGQLQLAASELSPQQLAWVSGYLWGVSQTSGATQPANQAAVAVSSEPVSKLSIIYAS
QTGNAKGVAEALKAEASAIGIRVELFDASDYKGKNLAKETHVIIVASTNGEGEAPDNGIELHEFLQSKKAPKLDKLHYGV
IGLGDSSYEFFCQTGKDFDGFLAKKGATAFIDRIDLDVDYEASAVEWRKQALEKVKETLSTDNESKNGQVVQLPVGQAAR
SASQYSKQNPYTATLLASQKITGRDSGKDVRHIEIDLEDSGLTYQPGDALGVWFENSLELVTAVLKQVGLTGEEHIEVDD
ESISLQKALIEKYEITSANPQQVSKFSELSGSKKLEKLVQDKDKLRQYAGNTQVIDLLSEKKTKLTAEQLVGLLRRLTPR
LYSIASSQSEVDEEVHLTVGVVEYQLGDETRFGGASSFLSHRLEEGAEVNVFIEHNNNFKLPQDDNVPVIMIGPGTGIAP
FRAFVQERDNRDAEGKNWLFFGDRTFTQDFLYQVEWQKYLKSGVINQLDVAFSRDQQEKVYVQHRILEHSIQVWQWLQDG
AYIYVCGDATRMAKDVHEALITIVEQQGKKNREEAEQFVNDLRKAKRYQRDVY

Specific function: Component of the sulfite reductase complex that catalyzes the 6-electron reduction of sulfite to sulfide. This is one of several activities required for the biosynthesis of L- cysteine from sulfate. The flavoprotein component catalyzes the electron flow f

COG id: COG0369

COG function: function code P; Sulfite reductase, alpha subunit (flavoprotein)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 flavodoxin-like domain [H]

Homologues:

Organism=Homo sapiens, GI7657393, Length=608, Percent_Identity=30.4276315789474, Blast_Score=228, Evalue=1e-59,
Organism=Homo sapiens, GI127139033, Length=600, Percent_Identity=29.3333333333333, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI221316709, Length=608, Percent_Identity=30.4276315789474, Blast_Score=224, Evalue=2e-58,
Organism=Homo sapiens, GI221316705, Length=617, Percent_Identity=29.9837925445705, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI24041029, Length=600, Percent_Identity=30.8333333333333, Blast_Score=210, Evalue=3e-54,
Organism=Homo sapiens, GI221316707, Length=536, Percent_Identity=31.5298507462687, Blast_Score=202, Evalue=6e-52,
Organism=Homo sapiens, GI10835173, Length=648, Percent_Identity=27.9320987654321, Blast_Score=187, Evalue=3e-47,
Organism=Homo sapiens, GI40254422, Length=656, Percent_Identity=27.5914634146341, Blast_Score=165, Evalue=1e-40,
Organism=Homo sapiens, GI169790958, Length=444, Percent_Identity=29.2792792792793, Blast_Score=162, Evalue=6e-40,
Organism=Homo sapiens, GI169790956, Length=444, Percent_Identity=29.0540540540541, Blast_Score=162, Evalue=7e-40,
Organism=Escherichia coli, GI1789123, Length=599, Percent_Identity=51.5859766277129, Blast_Score=619, Evalue=1e-178,
Organism=Escherichia coli, GI1789154, Length=117, Percent_Identity=32.4786324786325, Blast_Score=67, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17554134, Length=614, Percent_Identity=30.1302931596091, Blast_Score=248, Evalue=7e-66,
Organism=Caenorhabditis elegans, GI17566446, Length=588, Percent_Identity=27.3809523809524, Blast_Score=175, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17531441, Length=419, Percent_Identity=24.3436754176611, Blast_Score=100, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6321832, Length=659, Percent_Identity=30.1972685887709, Blast_Score=218, Evalue=2e-57,
Organism=Saccharomyces cerevisiae, GI6321143, Length=386, Percent_Identity=31.6062176165803, Blast_Score=150, Evalue=6e-37,
Organism=Saccharomyces cerevisiae, GI6325305, Length=638, Percent_Identity=22.884012539185, Blast_Score=131, Evalue=3e-31,
Organism=Saccharomyces cerevisiae, GI6322597, Length=153, Percent_Identity=31.3725490196078, Blast_Score=64, Evalue=7e-11,
Organism=Drosophila melanogaster, GI17137192, Length=599, Percent_Identity=28.2136894824708, Blast_Score=214, Evalue=1e-55,
Organism=Drosophila melanogaster, GI24660907, Length=598, Percent_Identity=29.0969899665552, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24660903, Length=598, Percent_Identity=29.0969899665552, Blast_Score=194, Evalue=1e-49,
Organism=Drosophila melanogaster, GI24582192, Length=512, Percent_Identity=29.1015625, Blast_Score=190, Evalue=2e-48,
Organism=Drosophila melanogaster, GI24583543, Length=747, Percent_Identity=26.6398929049531, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78706876, Length=747, Percent_Identity=26.6398929049531, Blast_Score=186, Evalue=5e-47,
Organism=Drosophila melanogaster, GI78706872, Length=747, Percent_Identity=26.6398929049531, Blast_Score=185, Evalue=6e-47,
Organism=Drosophila melanogaster, GI24647438, Length=390, Percent_Identity=24.8717948717949, Blast_Score=84, Evalue=2e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010199
- InterPro:   IPR003097
- InterPro:   IPR017927
- InterPro:   IPR001094
- InterPro:   IPR008254
- InterPro:   IPR001709
- InterPro:   IPR023173
- InterPro:   IPR001433
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00667 FAD_binding_1; PF00258 Flavodoxin_1; PF00175 NAD_binding_1 [H]

EC number: =1.8.1.2 [H]

Molecular weight: Translated: 68208; Mature: 68208

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: PS50902 FLAVODOXIN_LIKE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRDVVMLLKELSALASPLNDQQIGQLQLAASELSPQQLAWVSGYLWGVSQTSGATQPAN
CCCHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
QAAVAVSSEPVSKLSIIYASQTGNAKGVAEALKAEASAIGIRVELFDASDYKGKNLAKET
CEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCE
HVIIVASTNGEGEAPDNGIELHEFLQSKKAPKLDKLHYGVIGLGDSSYEFFCQTGKDFDG
EEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEECCCCCCEEEEECCCCHHH
FLAKKGATAFIDRIDLDVDYEASAVEWRKQALEKVKETLSTDNESKNGQVVQLPVGQAAR
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHH
SASQYSKQNPYTATLLASQKITGRDSGKDVRHIEIDLEDSGLTYQPGDALGVWFENSLEL
HHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCEECCCCEEEEEECCHHHH
VTAVLKQVGLTGEEHIEVDDESISLQKALIEKYEITSANPQQVSKFSELSGSKKLEKLVQ
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHH
DKDKLRQYAGNTQVIDLLSEKKTKLTAEQLVGLLRRLTPRLYSIASSQSEVDEEVHLTVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
VVEYQLGDETRFGGASSFLSHRLEEGAEVNVFIEHNNNFKLPQDDNVPVIMIGPGTGIAP
EEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHH
FRAFVQERDNRDAEGKNWLFFGDRTFTQDFLYQVEWQKYLKSGVINQLDVAFSRDQQEKV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
YVQHRILEHSIQVWQWLQDGAYIYVCGDATRMAKDVHEALITIVEQQGKKNREEAEQFVN
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DLRKAKRYQRDVY
HHHHHHHHHHCCC
>Mature Secondary Structure
MNRDVVMLLKELSALASPLNDQQIGQLQLAASELSPQQLAWVSGYLWGVSQTSGATQPAN
CCCHHHHHHHHHHHHHCCCCCCCCCHHEEHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCC
QAAVAVSSEPVSKLSIIYASQTGNAKGVAEALKAEASAIGIRVELFDASDYKGKNLAKET
CEEEEECCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHEEEEEEEEECCCCCCCCCCCCE
HVIIVASTNGEGEAPDNGIELHEFLQSKKAPKLDKLHYGVIGLGDSSYEFFCQTGKDFDG
EEEEEEECCCCCCCCCCCHHHHHHHHHCCCCCCHHEEEEEEECCCCCCEEEEECCCCHHH
FLAKKGATAFIDRIDLDVDYEASAVEWRKQALEKVKETLSTDNESKNGQVVQLPVGQAAR
HHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCHHHH
SASQYSKQNPYTATLLASQKITGRDSGKDVRHIEIDLEDSGLTYQPGDALGVWFENSLEL
HHHHHHCCCCCEEEEEECCCCCCCCCCCCEEEEEEEEECCCCEECCCCEEEEEECCHHHH
VTAVLKQVGLTGEEHIEVDDESISLQKALIEKYEITSANPQQVSKFSELSGSKKLEKLVQ
HHHHHHHHCCCCCCCEEECCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCHHHHHHHHH
DKDKLRQYAGNTQVIDLLSEKKTKLTAEQLVGLLRRLTPRLYSIASSQSEVDEEVHLTVG
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCEEEEEE
VVEYQLGDETRFGGASSFLSHRLEEGAEVNVFIEHNNNFKLPQDDNVPVIMIGPGTGIAP
EEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCCCCCCCEEEECCCCCHHH
FRAFVQERDNRDAEGKNWLFFGDRTFTQDFLYQVEWQKYLKSGVINQLDVAFSRDQQEKV
HHHHHHHHCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCCHHHH
YVQHRILEHSIQVWQWLQDGAYIYVCGDATRMAKDVHEALITIVEQQGKKNREEAEQFVN
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DLRKAKRYQRDVY
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA