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The map label for this gene is rfaF [H]

Identifier: 209693870

GI number: 209693870

Start: 308451

End: 309521

Strand: Reverse

Name: rfaF [H]

Synonym: VSAL_I0262

Alternate gene names: 209693870

Gene position: 309521-308451 (Counterclockwise)

Preceding gene: 209693871

Following gene: 209693869

Centisome position: 9.31

GC content: 41.83

Gene sequence:

>1071_bases
ATGAAAATTTTGATCATTGGCCCATCTTGGGTGGGTGATATGGTGATGTCGCAATCACTGTATATCACACTAAAACAACA
AAACCCTGACGCTGTTATTGATGTTATGGCTCCGGCATGGTGTAAGCCTATTTTGGAACGCATGCCTGAAGTGAACCAAG
CGATTGAAATGCCGCTTGGTCATGGTGCCTTTGATTTGTTTGGTCGCCGCCGTTTAAGTAAGCAGCTTAGCGCTAATAAT
TATACTCATGCTTATATCTGCCCTAATTCCGCTAAATCGGCATTAATACCTTGGTTTGCTGGTATTCCAATTCGTACTGG
CTGGAAAGGCGAGATGCGCTATGGCCTACTTAACGATTTACGTTCAAATAAGAAATCATTTCAATACATGGTCGAACGCT
ATGTGGCATTGGCTCATCCAAAATCAGAGATGATTAATTCAAGCTCATTAGGTGGGCTAGATACTTTACCTCGACCTAAG
CTTGCTATTGATGCTGATACCCAGCAAGCAATGTTAACTAAATTTTCTCTATCTGCGGATAAACCTGTTCTTGGCTTATG
CCCTGGTGCGGAATTTGGCCCGGCAAAACAGTGGCCTGTTGAGCATTACGCAGCTGTAGCTAATGAGATGATTAACCAAG
GCTATCAAGTATGGTTATTTGGTTCAGCAAAAGATAAAGAAACCACTGAAGCCATTAAATCTTTGGTTCATTCAGATAAT
CAAGCATCGGTATTTAACCTAGCGGGTGATACCAGTTTAATTGAAGCTGTAGATCTGCTTGCTGCTTGTAAAACGGTTGT
CAGTAATGACTCTGGCTTAATGCATGTAGCTGCTGCTGTCGGTTGTCATGTGGTTGGGGTTTATGGTTCAACATCACCAA
AGTACACGCCACCATTAGCTGAAAAAGTTGACATTGTGAGTACAGATATTGAATGTCGCCCTTGTTTTAAACGCGAGTGT
GAATTTAAGCATTTGAAGTGTCTGACCGATCTTGATCCACAACTAGTGCTGGATAAAATTAGTCCCAAAAGCCACTCGAA
TGAATCTGTCATTCAGGTTACTTCATGTTAA

Upstream 100 bases:

>100_bases
CCCTTACCCAACATTACTTATCACATCACTCAGGCATTTTATTCTTTGCCGTTGGCATCGCCATACTGAGCGCCTTAGTG
ATTAACCAAAGAAAACCATC

Downstream 100 bases:

>100_bases
TCCGACTAGTTTATACCTTACTATTAACACTTCTCTCTCCTGTTTTATTGTTTGGTTTATATAAGAAAAAACCAAACAAA
CCTCAATTTGGTGACCGCTG

Product: ADP-heptose-LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 356; Mature: 356

Protein sequence:

>356_residues
MKILIIGPSWVGDMVMSQSLYITLKQQNPDAVIDVMAPAWCKPILERMPEVNQAIEMPLGHGAFDLFGRRRLSKQLSANN
YTHAYICPNSAKSALIPWFAGIPIRTGWKGEMRYGLLNDLRSNKKSFQYMVERYVALAHPKSEMINSSSLGGLDTLPRPK
LAIDADTQQAMLTKFSLSADKPVLGLCPGAEFGPAKQWPVEHYAAVANEMINQGYQVWLFGSAKDKETTEAIKSLVHSDN
QASVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKREC
EFKHLKCLTDLDPQLVLDKISPKSHSNESVIQVTSC

Sequences:

>Translated_356_residues
MKILIIGPSWVGDMVMSQSLYITLKQQNPDAVIDVMAPAWCKPILERMPEVNQAIEMPLGHGAFDLFGRRRLSKQLSANN
YTHAYICPNSAKSALIPWFAGIPIRTGWKGEMRYGLLNDLRSNKKSFQYMVERYVALAHPKSEMINSSSLGGLDTLPRPK
LAIDADTQQAMLTKFSLSADKPVLGLCPGAEFGPAKQWPVEHYAAVANEMINQGYQVWLFGSAKDKETTEAIKSLVHSDN
QASVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKREC
EFKHLKCLTDLDPQLVLDKISPKSHSNESVIQVTSC
>Mature_356_residues
MKILIIGPSWVGDMVMSQSLYITLKQQNPDAVIDVMAPAWCKPILERMPEVNQAIEMPLGHGAFDLFGRRRLSKQLSANN
YTHAYICPNSAKSALIPWFAGIPIRTGWKGEMRYGLLNDLRSNKKSFQYMVERYVALAHPKSEMINSSSLGGLDTLPRPK
LAIDADTQQAMLTKFSLSADKPVLGLCPGAEFGPAKQWPVEHYAAVANEMINQGYQVWLFGSAKDKETTEAIKSLVHSDN
QASVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLAEKVDIVSTDIECRPCFKREC
EFKHLKCLTDLDPQLVLDKISPKSHSNESVIQVTSC

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=342, Percent_Identity=52.3391812865497, Blast_Score=367, Evalue=1e-103,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 39072; Mature: 39072

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
6.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILIIGPSWVGDMVMSQSLYITLKQQNPDAVIDVMAPAWCKPILERMPEVNQAIEMPLG
CEEEEECCCHHHHHHHCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHCCHHHHHHHCCCC
HGAFDLFGRRRLSKQLSANNYTHAYICPNSAKSALIPWFAGIPIRTGWKGEMRYGLLNDL
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
RSNKKSFQYMVERYVALAHPKSEMINSSSLGGLDTLPRPKLAIDADTQQAMLTKFSLSAD
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCC
KPVLGLCPGAEFGPAKQWPVEHYAAVANEMINQGYQVWLFGSAKDKETTEAIKSLVHSDN
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCC
QASVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLA
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEEECCCCCCCCCCHH
EKVDIVSTDIECRPCFKRECEFKHLKCLTDLDPQLVLDKISPKSHSNESVIQVTSC
HHCEEEECCCCCCCCHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MKILIIGPSWVGDMVMSQSLYITLKQQNPDAVIDVMAPAWCKPILERMPEVNQAIEMPLG
CEEEEECCCHHHHHHHCCEEEEEEECCCCCEEEEHHCHHHHHHHHHHCCHHHHHHHCCCC
HGAFDLFGRRRLSKQLSANNYTHAYICPNSAKSALIPWFAGIPIRTGWKGEMRYGLLNDL
CCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHHCCCCCCCCCCCCHHHHHHHHH
RSNKKSFQYMVERYVALAHPKSEMINSSSLGGLDTLPRPKLAIDADTQQAMLTKFSLSAD
HHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCC
KPVLGLCPGAEFGPAKQWPVEHYAAVANEMINQGYQVWLFGSAKDKETTEAIKSLVHSDN
CCEEEECCCCCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHCCCC
QASVFNLAGDTSLIEAVDLLAACKTVVSNDSGLMHVAAAVGCHVVGVYGSTSPKYTPPLA
CCEEEEECCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCEEEEEEECCCCCCCCCCHH
EKVDIVSTDIECRPCFKRECEFKHLKCLTDLDPQLVLDKISPKSHSNESVIQVTSC
HHCEEEECCCCCCCCHHHCCCHHHHHHHHCCCHHHHHCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]