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The map label for this gene is waaA [H]

Identifier: 209693869

GI number: 209693869

Start: 307195

End: 308457

Strand: Reverse

Name: waaA [H]

Synonym: VSAL_I0261

Alternate gene names: 209693869

Gene position: 308457-307195 (Counterclockwise)

Preceding gene: 209693870

Following gene: 209693868

Centisome position: 9.28

GC content: 40.06

Gene sequence:

>1263_bases
ATGTTAATCCGACTAGTTTATACCTTACTATTAACACTTCTCTCTCCTGTTTTATTGTTTGGTTTATATAAGAAAAAACC
AAACAAACCTCAATTTGGTGACCGCTGGAAGGAGCACTTTGGATTTACTCCTACGCTAAAAAAAAGCGAACAACCACTCT
GGATTCATGCGGTTTCAGTTGGAGAAGCGATTGCTGCGACACCATTAATTAAAGCATTAAAACAGCAGAGCCCTGAACAG
CCAATCCTAGTGACAACCACAACAAGCACGGGTGCAGAGCAGATTGCCAAGTTAGGTGATTTAGTTGAACACCGCTATAT
GCCTATTGATTTCCCATTTGCTGTGAACGGATTTTTAAAAACGGTTAATCCAAGTAAATTACTGATTATTGAAACAGAGC
TGTGGCCAAATACCTTACATTCTGTCGGTAAATCTAACATTCCTATTTATGTGGTTAACGCACGTTTATCTGAGAAGTCT
TGCCAAGGCTACACCAAAATTCAACCTGTTTTTACCGAGCTAAGTAAGCACTTAACAAAAGTACTATGCCAAACCCAAGC
GGATGCAGATCGCTTTGCGCGTTTAGGTATCAATAAAGAAAGATTATGTGCTACTGGCTCTATCAAATTTGATATTCAAA
TCTCGGATGAGATAAAACAGCAAGGCCAAGAGTTACGTCAGCTATTAGACAATGACCGCCCTATTTGGATTGCAGCTAGT
ACTCATAAAGGTGAAGATGAACAAGTTCTTGATGCTCATAAAGAAGTATTGAAAGAGCACCCTAACGCCTTGTTAATCTT
GGTACCTCGCCACCCTGAGCGTTTTGATACGGTATTTGAATTAAGTAAAAACTCGGGATTTAGCAGTATAAGACGCACCT
CTAACAAAACGGTTACCGCTGAAACTCAAGTTTATCTTGGTGATACCATGGGTGAAATGCTAGTTCTGATCGGCGCGGCT
GATGTGTGCTTTATGGGCGGGAGTTTACTTGGCGATAAAGTCGGTGGGCACAATGTTTTAGAGCCTGCTGCGCTAGGTGT
GCCTGTTATTATAGGGCCGAGTTATTATAACTTTAAAGAGATTGTGGATACGTTACGAGGTGAACAAGGGATTGTTATTT
GTGAGCCGACTCAACTTAGTAATCACATCGTCAAGCTGATTGATGATAGTACTGAATATCAATTACTTCAAAACTCCGCT
TCTAATGTTGTTCAAAGTAATCAAGGTGCTTTAAATCGTACGATCGCTATCTCAAATATATAA

Upstream 100 bases:

>100_bases
TTAAGCATTTGAAGTGTCTGACCGATCTTGATCCACAACTAGTGCTGGATAAAATTAGTCCCAAAAGCCACTCGAATGAA
TCTGTCATTCAGGTTACTTC

Downstream 100 bases:

>100_bases
TCACGATTATCAGTAGTCATATCTAAATTAAATAAGGTTAATTTTCATGAATGTTTTTCTTGTGACATCCCCCTTTCAAT
ATATTTGTGCAAATGAAGCA

Product: 3-deoxy-D-manno-octulosonic-acid transferase

Products: NA

Alternate protein names: KDO transferase [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MLIRLVYTLLLTLLSPVLLFGLYKKKPNKPQFGDRWKEHFGFTPTLKKSEQPLWIHAVSVGEAIAATPLIKALKQQSPEQ
PILVTTTTSTGAEQIAKLGDLVEHRYMPIDFPFAVNGFLKTVNPSKLLIIETELWPNTLHSVGKSNIPIYVVNARLSEKS
CQGYTKIQPVFTELSKHLTKVLCQTQADADRFARLGINKERLCATGSIKFDIQISDEIKQQGQELRQLLDNDRPIWIAAS
THKGEDEQVLDAHKEVLKEHPNALLILVPRHPERFDTVFELSKNSGFSSIRRTSNKTVTAETQVYLGDTMGEMLVLIGAA
DVCFMGGSLLGDKVGGHNVLEPAALGVPVIIGPSYYNFKEIVDTLRGEQGIVICEPTQLSNHIVKLIDDSTEYQLLQNSA
SNVVQSNQGALNRTIAISNI

Sequences:

>Translated_420_residues
MLIRLVYTLLLTLLSPVLLFGLYKKKPNKPQFGDRWKEHFGFTPTLKKSEQPLWIHAVSVGEAIAATPLIKALKQQSPEQ
PILVTTTTSTGAEQIAKLGDLVEHRYMPIDFPFAVNGFLKTVNPSKLLIIETELWPNTLHSVGKSNIPIYVVNARLSEKS
CQGYTKIQPVFTELSKHLTKVLCQTQADADRFARLGINKERLCATGSIKFDIQISDEIKQQGQELRQLLDNDRPIWIAAS
THKGEDEQVLDAHKEVLKEHPNALLILVPRHPERFDTVFELSKNSGFSSIRRTSNKTVTAETQVYLGDTMGEMLVLIGAA
DVCFMGGSLLGDKVGGHNVLEPAALGVPVIIGPSYYNFKEIVDTLRGEQGIVICEPTQLSNHIVKLIDDSTEYQLLQNSA
SNVVQSNQGALNRTIAISNI
>Mature_420_residues
MLIRLVYTLLLTLLSPVLLFGLYKKKPNKPQFGDRWKEHFGFTPTLKKSEQPLWIHAVSVGEAIAATPLIKALKQQSPEQ
PILVTTTTSTGAEQIAKLGDLVEHRYMPIDFPFAVNGFLKTVNPSKLLIIETELWPNTLHSVGKSNIPIYVVNARLSEKS
CQGYTKIQPVFTELSKHLTKVLCQTQADADRFARLGINKERLCATGSIKFDIQISDEIKQQGQELRQLLDNDRPIWIAAS
THKGEDEQVLDAHKEVLKEHPNALLILVPRHPERFDTVFELSKNSGFSSIRRTSNKTVTAETQVYLGDTMGEMLVLIGAA
DVCFMGGSLLGDKVGGHNVLEPAALGVPVIIGPSYYNFKEIVDTLRGEQGIVICEPTQLSNHIVKLIDDSTEYQLLQNSA
SNVVQSNQGALNRTIAISNI

Specific function: Essential step in lipopolysaccharides biosynthesis. Acts at transfer of 3-deoxy-D-mono octulonic acid (KDO) from CMP-KDO to a tetraacyldisaccharide 1,4'-bisphosphate precursor of lipid A (lipid IVA). Transfers two molecules of KDO to lipid IVA [H]

COG id: COG1519

COG function: function code M; 3-deoxy-D-manno-octulosonic-acid transferase

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 1 family [H]

Homologues:

Organism=Escherichia coli, GI1790064, Length=420, Percent_Identity=40.4761904761905, Blast_Score=339, Evalue=3e-94,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001296
- InterPro:   IPR007507 [H]

Pfam domain/function: PF00534 Glycos_transf_1; PF04413 Glycos_transf_N [H]

EC number: 2.-.-.-

Molecular weight: Translated: 46585; Mature: 46585

Theoretical pI: Translated: 7.34; Mature: 7.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIRLVYTLLLTLLSPVLLFGLYKKKPNKPQFGDRWKEHFGFTPTLKKSEQPLWIHAVSV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEC
GEAIAATPLIKALKQQSPEQPILVTTTTSTGAEQIAKLGDLVEHRYMPIDFPFAVNGFLK
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHC
TVNPSKLLIIETELWPNTLHSVGKSNIPIYVVNARLSEKSCQGYTKIQPVFTELSKHLTK
CCCCCEEEEEEECCCCHHHHHCCCCCCCEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHH
VLCQTQADADRFARLGINKERLCATGSIKFDIQISDEIKQQGQELRQLLDNDRPIWIAAS
HHHHCCCCHHHHHHCCCCHHHEEEECCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEC
THKGEDEQVLDAHKEVLKEHPNALLILVPRHPERFDTVFELSKNSGFSSIRRTSNKTVTA
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEE
ETQVYLGDTMGEMLVLIGAADVCFMGGSLLGDKVGGHNVLEPAALGVPVIIGPSYYNFKE
EEEEEECCHHHHHHHHHCCHHHHHHCCCHHCCCCCCCCCCCHHHCCCCEEECCCCCCHHH
IVDTLRGEQGIVICEPTQLSNHIVKLIDDSTEYQLLQNSASNVVQSNQGALNRTIAISNI
HHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC
>Mature Secondary Structure
MLIRLVYTLLLTLLSPVLLFGLYKKKPNKPQFGDRWKEHFGFTPTLKKSEQPLWIHAVSV
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCCCCCCCCCCCEEEEEEEC
GEAIAATPLIKALKQQSPEQPILVTTTTSTGAEQIAKLGDLVEHRYMPIDFPFAVNGFLK
CCHHHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCHHC
TVNPSKLLIIETELWPNTLHSVGKSNIPIYVVNARLSEKSCQGYTKIQPVFTELSKHLTK
CCCCCEEEEEEECCCCHHHHHCCCCCCCEEEEECCCCCHHCCCCHHHHHHHHHHHHHHHH
VLCQTQADADRFARLGINKERLCATGSIKFDIQISDEIKQQGQELRQLLDNDRPIWIAAS
HHHHCCCCHHHHHHCCCCHHHEEEECCEEEEEEECHHHHHHHHHHHHHHCCCCCEEEEEC
THKGEDEQVLDAHKEVLKEHPNALLILVPRHPERFDTVFELSKNSGFSSIRRTSNKTVTA
CCCCCCHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHCCCCHHHHHHCCCCEEEE
ETQVYLGDTMGEMLVLIGAADVCFMGGSLLGDKVGGHNVLEPAALGVPVIIGPSYYNFKE
EEEEEECCHHHHHHHHHCCHHHHHHCCCHHCCCCCCCCCCCHHHCCCCEEECCCCCCHHH
IVDTLRGEQGIVICEPTQLSNHIVKLIDDSTEYQLLQNSASNVVQSNQGALNRTIAISNI
HHHHHCCCCCEEEECCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]