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The map label for this gene is hemC

Identifier: 209693832

GI number: 209693832

Start: 260447

End: 261382

Strand: Reverse

Name: hemC

Synonym: VSAL_I0220b

Alternate gene names: 209693832

Gene position: 261382-260447 (Counterclockwise)

Preceding gene: 209693835

Following gene: 209693831

Centisome position: 7.86

GC content: 44.44

Gene sequence:

>936_bases
ATGTCACAACAATTACCGGTTCGTATTGCCACTCGTAAAAGTCCTCTGGCTCTATGGCAAGCTCATTTTGTAAAGGATGC
ACTGCAAGCCGCTCACCCTGGTTTGGAAGTCGAACTCGTTACAATGGTCACTAAAGGAGACATCATTCTTGATACACCTT
TAGCTAAAATTGGCGGTAAAGGTTTATTCGTTAAGGAGCTTGAAGTTGCGATGTTAGAAGGTCGTGCCGATCTTGCCGTT
CACTCTATGAAAGACGTGCCTGTCGAATTTCCTGAAGGACTAGGACTTGTCACTATTTGCGAGCGTGGAGACCCGCGTGA
TGCTTTTGTATCAAATACCTATAACAACATTGATGAATTACCACAAGGTGCTACAGTTGGTACATGTAGCTTACGTCGTC
AATGCCAGTTAAAAGAATATCGTCCAGATTTAATCATCAAAGAATTACGCGGTAATGTGGGAACACGTTTACAAAAACTG
GATGATGGTAATTACGATGCGATCATTCTTGCGTGTGCCGGACTGATTCGTCTTGGCTTAGAAGATCGTATTAAAAGTTC
AATTGAACCAGAGCAATCTCTACCGGCTGTTGGTCAAGGTGCTGTTGGTATTGAAACCCGCTTGAACGATGATCGTATTC
GAGCACTACTCGCACCACTTAATCACCCAGAAACGGCTCACCGTGTATTGTGTGAAAGAGCAATGAATAATCGTCTTGAA
GGCGGTTGCCAGGTACCGATTGGCAGCTATTCATTAATCGATGGCGATCAAATTTGGTTACGCGCTTTAGTTGGTGAACC
TGACGGGTCGATTATGGTTCGCGGTGAAACTACAGGTCCAGTCTCTGATGCTGAAGAACTTGGCACAAAACTGGCTGAAC
AACTGCTTAATGACGGTGCAAAAGACATTTTAGACCGCCTATACGCTGACGCTTAA

Upstream 100 bases:

>100_bases
GTGTTGTTTTTAACCTATAATCACACTGTTGTCATCCGGTATTTTCACCTCACCCTCAACTAATGGTAGGATAAGGTAAT
TATTTTGAACGGATACTACT

Downstream 100 bases:

>100_bases
CATGACCATATTGGTGACTCGCCCAGAGCAAGAGAGCCAAGCACTCTGTGATGCCCTACACCAAGCAGGGCACCTCGCCA
TTTCTCATCCTTTGTTAACC

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 311; Mature: 310

Protein sequence:

>311_residues
MSQQLPVRIATRKSPLALWQAHFVKDALQAAHPGLEVELVTMVTKGDIILDTPLAKIGGKGLFVKELEVAMLEGRADLAV
HSMKDVPVEFPEGLGLVTICERGDPRDAFVSNTYNNIDELPQGATVGTCSLRRQCQLKEYRPDLIIKELRGNVGTRLQKL
DDGNYDAIILACAGLIRLGLEDRIKSSIEPEQSLPAVGQGAVGIETRLNDDRIRALLAPLNHPETAHRVLCERAMNNRLE
GGCQVPIGSYSLIDGDQIWLRALVGEPDGSIMVRGETTGPVSDAEELGTKLAEQLLNDGAKDILDRLYADA

Sequences:

>Translated_311_residues
MSQQLPVRIATRKSPLALWQAHFVKDALQAAHPGLEVELVTMVTKGDIILDTPLAKIGGKGLFVKELEVAMLEGRADLAV
HSMKDVPVEFPEGLGLVTICERGDPRDAFVSNTYNNIDELPQGATVGTCSLRRQCQLKEYRPDLIIKELRGNVGTRLQKL
DDGNYDAIILACAGLIRLGLEDRIKSSIEPEQSLPAVGQGAVGIETRLNDDRIRALLAPLNHPETAHRVLCERAMNNRLE
GGCQVPIGSYSLIDGDQIWLRALVGEPDGSIMVRGETTGPVSDAEELGTKLAEQLLNDGAKDILDRLYADA
>Mature_310_residues
SQQLPVRIATRKSPLALWQAHFVKDALQAAHPGLEVELVTMVTKGDIILDTPLAKIGGKGLFVKELEVAMLEGRADLAVH
SMKDVPVEFPEGLGLVTICERGDPRDAFVSNTYNNIDELPQGATVGTCSLRRQCQLKEYRPDLIIKELRGNVGTRLQKLD
DGNYDAIILACAGLIRLGLEDRIKSSIEPEQSLPAVGQGAVGIETRLNDDRIRALLAPLNHPETAHRVLCERAMNNRLEG
GCQVPIGSYSLIDGDQIWLRALVGEPDGSIMVRGETTGPVSDAEELGTKLAEQLLNDGAKDILDRLYADA

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI66933009, Length=332, Percent_Identity=41.2650602409639, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI20149500, Length=332, Percent_Identity=41.2650602409639, Blast_Score=232, Evalue=3e-61,
Organism=Escherichia coli, GI48994974, Length=303, Percent_Identity=74.9174917491749, Blast_Score=455, Evalue=1e-129,
Organism=Saccharomyces cerevisiae, GI6319996, Length=319, Percent_Identity=40.1253918495298, Blast_Score=204, Evalue=1e-53,
Organism=Drosophila melanogaster, GI20130425, Length=287, Percent_Identity=40.418118466899, Blast_Score=200, Evalue=1e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_ALISL (B6EPK1)

Other databases:

- EMBL:   FM178379
- RefSeq:   YP_002261760.1
- GeneID:   6988643
- GenomeReviews:   FM178379_GR
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 33829; Mature: 33698

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQQLPVRIATRKSPLALWQAHFVKDALQAAHPGLEVELVTMVTKGDIILDTPLAKIGGK
CCCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCHHHHCCC
GLFVKELEVAMLEGRADLAVHSMKDVPVEFPEGLGLVTICERGDPRDAFVSNTYNNIDEL
CEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCHHHHHHC
PQGATVGTCSLRRQCQLKEYRPDLIIKELRGNVGTRLQKLDDGNYDAIILACAGLIRLGL
CCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCH
EDRIKSSIEPEQSLPAVGQGAVGIETRLNDDRIRALLAPLNHPETAHRVLCERAMNNRLE
HHHHHHCCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GGCQVPIGSYSLIDGDQIWLRALVGEPDGSIMVRGETTGPVSDAEELGTKLAEQLLNDGA
CCEECCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCH
KDILDRLYADA
HHHHHHHHCCC
>Mature Secondary Structure 
SQQLPVRIATRKSPLALWQAHFVKDALQAAHPGLEVELVTMVTKGDIILDTPLAKIGGK
CCCCCEEEECCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCEEEECCHHHHCCC
GLFVKELEVAMLEGRADLAVHSMKDVPVEFPEGLGLVTICERGDPRDAFVSNTYNNIDEL
CEEHHHHHHHHHCCCHHHHHHHHCCCCCCCCCCCCEEEEECCCCCCHHHHHCCHHHHHHC
PQGATVGTCSLRRQCQLKEYRPDLIIKELRGNVGTRLQKLDDGNYDAIILACAGLIRLGL
CCCCEEEHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHCH
EDRIKSSIEPEQSLPAVGQGAVGIETRLNDDRIRALLAPLNHPETAHRVLCERAMNNRLE
HHHHHHCCCCHHCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCC
GGCQVPIGSYSLIDGDQIWLRALVGEPDGSIMVRGETTGPVSDAEELGTKLAEQLLNDGA
CCEECCCCCEEEECCHHHHHHHHHCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCH
KDILDRLYADA
HHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA