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The map label for this gene is lcfB [H]

Identifier: 209552176

GI number: 209552176

Start: 285949

End: 287550

Strand: Reverse

Name: lcfB [H]

Synonym: Rleg2_6334

Alternate gene names: 209552176

Gene position: 287550-285949 (Counterclockwise)

Preceding gene: 209552177

Following gene: 209552175

Centisome position: 93.13

GC content: 54.99

Gene sequence:

>1602_bases
ATGTTTTCAGATGAAACAAACAGGCTGCTTGCCCGAGGTTCCGCGATCAGTCTCGGGGACGTTCTAGAAACCCGGGTCAT
GGTATCGCCGGACTGGCTGGCACTCGAATATTTAGGTCACGATTTTACCTACAAAGAACTTAACACGCTTGCTAACCAGT
GTGCGTGGTCGTTGCTCGATCTCGGTATCAGATATGGAGACGCGGTTGTCGTCCTTTCGGAAAACACGCTCGATTATCTG
TCGCTGATCTACGGGGCGGCCAAGACTGGCGTCGCCGTTGCGGGATGCAACTATCGCCTTGCGGCGCCCGAGGTCGCGAG
GTCGATTTCTGTAATCGCTCCTCGGTACGTCTTCGTTTGTGCTTCGCTCGAGCCGCTATTGCGCGACGCAATGCCCCACT
ACCCATCCGATATCGTCGCCCCGGAGATAATTGTCTGGGACAAGAACGATTTCGGTGGAGCCAAACCTGCATTGAGTAGA
TCGCTGCAAAACGCCAAAACGCATAATCCGCGCATTGAAGTAGACCCTGAAGAAGTCCTCGTGATTGTTTATACGAGTGG
CACGACGGGAGCGCCGAAGGGGGCCGCCCTGTCGCACCGTGCGATAATGGCTCGCGCAGGAATTATGTGTGCCGAACTCC
ACCTAACTGAGCATGACAACTACGTAGCGTGGCACCCGCTTTTTCACATGTCCTGCTCCGATTACGTCCTCATCACACAC
GTCCGCGGCGGCAAGGTATTCATGACTCCGCGTTTCGACGCTCATGCGATAGCTGATTTTTGCGTTCGCGAGAAAATCGG
TTGGCTTTTCCTGGTGCCCGGTGTTCTCGATGAAGTTGCGGATGCGATCAAGGCAAGTGGCAAGCCGGTCGCAGGCGTCA
AATATGTCGGATGTATGGCAGACCTTTCCCCCGTTCATTCACTGCAAAACATCACCGAGGTCACCGGAGCAGGCTATTTC
AACACCTTCGGAACGACTGAGGTCGGCACTGTTCCAAGTGCCTATGGTACGCTGGACCTTGATCACACGCCGGTCTCGTT
CCGAAAGGTGCAATCGACCTTCTCGCGCATGCGGATCGTGGATCCGGACGGGAAAGATTGCCCGTTCGGCACTCCGGGCG
AAATCCTTTACCGCACTCCCACCCTGTTCAGCGGGTACTGGAACAACGACAAGGCCACCAATGAAACGATGCGTGACGGC
TGGTACCACTCCGGTGATGTGTGCGTCCTCTACGAGGACGGCACCTACGACTTCCTCGGTCGCAGCAAATATATGATCAA
ATCCGGCGGTGAAAGCATCTATCCGGCGGAAGTGGAGCATGTCCTTTTGAAGCATCCGAAGATTTCGGAGGTGCAGGTGA
TCCGTGTGCCGGACGAAAAGTGGTCCGAGGTCCCCGCCGCGTATATCGCAACTCACGACGGCCAGGCTATTTCGGTACAT
GAACTCAACGATTTCTGTGCTGGACACATAACAAAGTGGAAGGTTCCCAAGCACTACCGATTCATCAGCACGGATGACTT
CCCAAGGAACGTGACCGGAAAGATAGAGCGTCCGCTCTTGGAGAAGATGTTTGCCGCGGAGCAACTCGAGATTACCTCCT
GA

Upstream 100 bases:

>100_bases
CGTTTGGCCGTCGAGACCACTCAGGGCGAATAAGCCAAATATCAATCCCTGAACTAAGCATTTTCGATGCTGCCAGACTG
ACTGCAAGGAAGAAAAGATC

Downstream 100 bases:

>100_bases
ACCACGAAGGACGGAATCCCTCGCTTGCCGTCCCATCAGGGTTGGTTTACACATGAATGTTCTATTGCACTACAATCGGC
CGGCGACCTCCACGCCGGTC

Product: AMP-dependent synthetase and ligase

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 533; Mature: 533

Protein sequence:

>533_residues
MFSDETNRLLARGSAISLGDVLETRVMVSPDWLALEYLGHDFTYKELNTLANQCAWSLLDLGIRYGDAVVVLSENTLDYL
SLIYGAAKTGVAVAGCNYRLAAPEVARSISVIAPRYVFVCASLEPLLRDAMPHYPSDIVAPEIIVWDKNDFGGAKPALSR
SLQNAKTHNPRIEVDPEEVLVIVYTSGTTGAPKGAALSHRAIMARAGIMCAELHLTEHDNYVAWHPLFHMSCSDYVLITH
VRGGKVFMTPRFDAHAIADFCVREKIGWLFLVPGVLDEVADAIKASGKPVAGVKYVGCMADLSPVHSLQNITEVTGAGYF
NTFGTTEVGTVPSAYGTLDLDHTPVSFRKVQSTFSRMRIVDPDGKDCPFGTPGEILYRTPTLFSGYWNNDKATNETMRDG
WYHSGDVCVLYEDGTYDFLGRSKYMIKSGGESIYPAEVEHVLLKHPKISEVQVIRVPDEKWSEVPAAYIATHDGQAISVH
ELNDFCAGHITKWKVPKHYRFISTDDFPRNVTGKIERPLLEKMFAAEQLEITS

Sequences:

>Translated_533_residues
MFSDETNRLLARGSAISLGDVLETRVMVSPDWLALEYLGHDFTYKELNTLANQCAWSLLDLGIRYGDAVVVLSENTLDYL
SLIYGAAKTGVAVAGCNYRLAAPEVARSISVIAPRYVFVCASLEPLLRDAMPHYPSDIVAPEIIVWDKNDFGGAKPALSR
SLQNAKTHNPRIEVDPEEVLVIVYTSGTTGAPKGAALSHRAIMARAGIMCAELHLTEHDNYVAWHPLFHMSCSDYVLITH
VRGGKVFMTPRFDAHAIADFCVREKIGWLFLVPGVLDEVADAIKASGKPVAGVKYVGCMADLSPVHSLQNITEVTGAGYF
NTFGTTEVGTVPSAYGTLDLDHTPVSFRKVQSTFSRMRIVDPDGKDCPFGTPGEILYRTPTLFSGYWNNDKATNETMRDG
WYHSGDVCVLYEDGTYDFLGRSKYMIKSGGESIYPAEVEHVLLKHPKISEVQVIRVPDEKWSEVPAAYIATHDGQAISVH
ELNDFCAGHITKWKVPKHYRFISTDDFPRNVTGKIERPLLEKMFAAEQLEITS
>Mature_533_residues
MFSDETNRLLARGSAISLGDVLETRVMVSPDWLALEYLGHDFTYKELNTLANQCAWSLLDLGIRYGDAVVVLSENTLDYL
SLIYGAAKTGVAVAGCNYRLAAPEVARSISVIAPRYVFVCASLEPLLRDAMPHYPSDIVAPEIIVWDKNDFGGAKPALSR
SLQNAKTHNPRIEVDPEEVLVIVYTSGTTGAPKGAALSHRAIMARAGIMCAELHLTEHDNYVAWHPLFHMSCSDYVLITH
VRGGKVFMTPRFDAHAIADFCVREKIGWLFLVPGVLDEVADAIKASGKPVAGVKYVGCMADLSPVHSLQNITEVTGAGYF
NTFGTTEVGTVPSAYGTLDLDHTPVSFRKVQSTFSRMRIVDPDGKDCPFGTPGEILYRTPTLFSGYWNNDKATNETMRDG
WYHSGDVCVLYEDGTYDFLGRSKYMIKSGGESIYPAEVEHVLLKHPKISEVQVIRVPDEKWSEVPAAYIATHDGQAISVH
ELNDFCAGHITKWKVPKHYRFISTDDFPRNVTGKIERPLLEKMFAAEQLEITS

Specific function: Involved in the degradation of long-chain fatty acids [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI156151445, Length=551, Percent_Identity=26.6787658802178, Blast_Score=169, Evalue=7e-42,
Organism=Homo sapiens, GI187761345, Length=381, Percent_Identity=27.2965879265092, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI187761343, Length=381, Percent_Identity=27.2965879265092, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI58082049, Length=501, Percent_Identity=24.1516966067864, Blast_Score=114, Evalue=3e-25,
Organism=Homo sapiens, GI157311624, Length=504, Percent_Identity=23.8095238095238, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI157311622, Length=504, Percent_Identity=23.8095238095238, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI122937307, Length=503, Percent_Identity=23.8568588469185, Blast_Score=103, Evalue=3e-22,
Organism=Homo sapiens, GI115511026, Length=504, Percent_Identity=24.0079365079365, Blast_Score=101, Evalue=2e-21,
Organism=Homo sapiens, GI42544132, Length=503, Percent_Identity=23.6580516898608, Blast_Score=99, Evalue=8e-21,
Organism=Homo sapiens, GI38505220, Length=554, Percent_Identity=22.3826714801444, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI227499619, Length=501, Percent_Identity=22.5548902195609, Blast_Score=68, Evalue=2e-11,
Organism=Homo sapiens, GI62865631, Length=228, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI13325055, Length=228, Percent_Identity=24.5614035087719, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788107, Length=539, Percent_Identity=24.4897959183673, Blast_Score=144, Evalue=1e-35,
Organism=Escherichia coli, GI145693145, Length=525, Percent_Identity=24.7619047619048, Blast_Score=142, Evalue=5e-35,
Organism=Escherichia coli, GI221142682, Length=489, Percent_Identity=24.9488752556237, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788595, Length=357, Percent_Identity=23.249299719888, Blast_Score=84, Evalue=3e-17,
Organism=Escherichia coli, GI1786810, Length=520, Percent_Identity=23.2692307692308, Blast_Score=83, Evalue=5e-17,
Organism=Escherichia coli, GI1786801, Length=451, Percent_Identity=21.9512195121951, Blast_Score=73, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI71994690, Length=547, Percent_Identity=27.0566727605119, Blast_Score=192, Evalue=3e-49,
Organism=Caenorhabditis elegans, GI71994703, Length=547, Percent_Identity=27.0566727605119, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI71994694, Length=547, Percent_Identity=27.0566727605119, Blast_Score=192, Evalue=4e-49,
Organism=Caenorhabditis elegans, GI17559526, Length=514, Percent_Identity=28.0155642023346, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560308, Length=520, Percent_Identity=26.9230769230769, Blast_Score=146, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17558820, Length=505, Percent_Identity=27.1287128712871, Blast_Score=140, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI17557194, Length=520, Percent_Identity=25.5769230769231, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17560140, Length=492, Percent_Identity=23.3739837398374, Blast_Score=128, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI17538037, Length=513, Percent_Identity=25.5360623781676, Blast_Score=120, Evalue=2e-27,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=26.6, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996755, Length=461, Percent_Identity=23.2104121475054, Blast_Score=103, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17531443, Length=509, Percent_Identity=22.9862475442043, Blast_Score=102, Evalue=4e-22,
Organism=Caenorhabditis elegans, GI71985884, Length=385, Percent_Identity=23.8961038961039, Blast_Score=95, Evalue=1e-19,
Organism=Caenorhabditis elegans, GI115534440, Length=526, Percent_Identity=23.7642585551331, Blast_Score=88, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17551278, Length=167, Percent_Identity=32.9341317365269, Blast_Score=77, Evalue=3e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=449, Percent_Identity=27.1714922048998, Blast_Score=135, Evalue=1e-32,
Organism=Drosophila melanogaster, GI24581924, Length=526, Percent_Identity=26.9961977186312, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=24.2, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI18859661, Length=512, Percent_Identity=25.5859375, Blast_Score=122, Evalue=5e-28,
Organism=Drosophila melanogaster, GI20130357, Length=349, Percent_Identity=24.0687679083095, Blast_Score=111, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24656500, Length=527, Percent_Identity=23.719165085389, Blast_Score=108, Evalue=8e-24,
Organism=Drosophila melanogaster, GI21356441, Length=528, Percent_Identity=23.1060606060606, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24653035, Length=500, Percent_Identity=24.4, Blast_Score=105, Evalue=9e-23,
Organism=Drosophila melanogaster, GI161076582, Length=507, Percent_Identity=24.0631163708087, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24648257, Length=361, Percent_Identity=27.7008310249307, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI21356947, Length=503, Percent_Identity=22.6640159045726, Blast_Score=102, Evalue=9e-22,
Organism=Drosophila melanogaster, GI24648253, Length=503, Percent_Identity=23.6580516898608, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI24648255, Length=503, Percent_Identity=23.6580516898608, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI19922652, Length=570, Percent_Identity=21.5789473684211, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI45550496, Length=541, Percent_Identity=23.6598890942699, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI45552801, Length=541, Percent_Identity=23.6598890942699, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI24648260, Length=494, Percent_Identity=20.6477732793522, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI281365686, Length=524, Percent_Identity=20.2290076335878, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583465, Length=460, Percent_Identity=23.4782608695652, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI24583463, Length=460, Percent_Identity=23.4782608695652, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI281364798, Length=460, Percent_Identity=23.4782608695652, Blast_Score=78, Evalue=2e-14,
Organism=Drosophila melanogaster, GI221329755, Length=116, Percent_Identity=27.5862068965517, Blast_Score=69, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020459
- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 58934; Mature: 58934

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFSDETNRLLARGSAISLGDVLETRVMVSPDWLALEYLGHDFTYKELNTLANQCAWSLLD
CCCCCCCHHHHCCCCCCHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LGIRYGDAVVVLSENTLDYLSLIYGAAKTGVAVAGCNYRLAAPEVARSISVIAPRYVFVC
HHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCEEECCHHHHHHHHHHCCCEEEEE
ASLEPLLRDAMPHYPSDIVAPEIIVWDKNDFGGAKPALSRSLQNAKTHNPRIEVDPEEVL
CCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHEE
VIVYTSGTTGAPKGAALSHRAIMARAGIMCAELHLTEHDNYVAWHPLFHMSCSDYVLITH
EEEEECCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCEEEECCEEECCCCCEEEEEE
VRGGKVFMTPRFDAHAIADFCVREKIGWLFLVPGVLDEVADAIKASGKPVAGVKYVGCMA
ECCCEEEECCCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHHH
DLSPVHSLQNITEVTGAGYFNTFGTTEVGTVPSAYGTLDLDHTPVSFRKVQSTFSRMRIV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEEE
DPDGKDCPFGTPGEILYRTPTLFSGYWNNDKATNETMRDGWYHSGDVCVLYEDGTYDFLG
CCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCHHHHCCCEECCCEEEEEECCCEECCC
RSKYMIKSGGESIYPAEVEHVLLKHPKISEVQVIRVPDEKWSEVPAAYIATHDGQAISVH
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCEEEEEECCCCEEEEE
ELNDFCAGHITKWKVPKHYRFISTDDFPRNVTGKIERPLLEKMFAAEQLEITS
CCHHHHCCCCEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECC
>Mature Secondary Structure
MFSDETNRLLARGSAISLGDVLETRVMVSPDWLALEYLGHDFTYKELNTLANQCAWSLLD
CCCCCCCHHHHCCCCCCHHHHHHHEEEECCCEEEEEECCCCCCHHHHHHHHHHHHHHHHH
LGIRYGDAVVVLSENTLDYLSLIYGAAKTGVAVAGCNYRLAAPEVARSISVIAPRYVFVC
HHHCCCCEEEEEECCCHHHHHHHHHHHHCCEEEECCCEEECCHHHHHHHHHHCCCEEEEE
ASLEPLLRDAMPHYPSDIVAPEIIVWDKNDFGGAKPALSRSLQNAKTHNPRIEVDPEEVL
CCHHHHHHHCCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHHHHHHCCCCEEEECHHHEE
VIVYTSGTTGAPKGAALSHRAIMARAGIMCAELHLTEHDNYVAWHPLFHMSCSDYVLITH
EEEEECCCCCCCCCCCHHHHHHHHHCCEEEEEEEEECCCCEEEECCEEECCCCCEEEEEE
VRGGKVFMTPRFDAHAIADFCVREKIGWLFLVPGVLDEVADAIKASGKPVAGVKYVGCMA
ECCCEEEECCCCCHHHHHHHHHHHHCCEEEECCHHHHHHHHHHHCCCCCCCCEEEEHHHH
DLSPVHSLQNITEVTGAGYFNTFGTTEVGTVPSAYGTLDLDHTPVSFRKVQSTFSRMRIV
HHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHHHHHHHEEEE
DPDGKDCPFGTPGEILYRTPTLFSGYWNNDKATNETMRDGWYHSGDVCVLYEDGTYDFLG
CCCCCCCCCCCCCCEEEECCCEEECCCCCCCCCCHHHHCCCEECCCEEEEEECCCEECCC
RSKYMIKSGGESIYPAEVEHVLLKHPKISEVQVIRVPDEKWSEVPAAYIATHDGQAISVH
CCEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEECCCHHHHHCCEEEEEECCCCEEEEE
ELNDFCAGHITKWKVPKHYRFISTDDFPRNVTGKIERPLLEKMFAAEQLEITS
CCHHHHCCCCEEECCCCCEEEECCCCCCCCCCCCHHHHHHHHHHHHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]