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The map label for this gene is hyuA [H]

Identifier: 209552121

GI number: 209552121

Start: 216657

End: 218720

Strand: Reverse

Name: hyuA [H]

Synonym: Rleg2_6272

Alternate gene names: 209552121

Gene position: 218720-216657 (Counterclockwise)

Preceding gene: 209552122

Following gene: 209552120

Centisome position: 70.84

GC content: 58.33

Gene sequence:

>2064_bases
ATGAGCTATCGCATCGGCGTCGATATTGGCGGCACATTTACCGACTTCTCGGTACTCGATCAGAACGGAAAGACTTTTCT
CTGGAAGGAAGACAGCGATCCCGTTTTTCCGGCCCAGGCAATTCAGCGAGGACTAAAGGCTGTAGCACGCGAGCTCGGCC
ATACGACGGAAGAATTTCTGAAGAAGGTGTCGTTGTTCGTTCATGGAACCACGATTGCAACCAACCTCCTCATTCAGCGT
AACGGCCCGCGCATTGGCTTGCTCTGCACGGAAGGCTTCCGCGACATTCTGTATACCCGCGATGCCTTCAAGCCAGATCG
TTATAATCTGCGTATGCCCTATCCCGAACCCCTTGTGGACCGGTATCTTCGATTGGGTGTCAGGGAGCGCGTCAACAAAG
ACGGCGACATCATTACCCCGCTTGACGAAGAGGGCGTGCGGCAGGCGGCAAGGAGCATGCGTGACGCCGGTGTGTCTTCG
GTCGCGGTCGCGTTCCTGTGGTCGATTGCCAATTCCGAGCACGAATACAGAGCGGCGGAAATTCTGCGCGAGGAACTTCC
TAATGTTCCGGTGCTGTGCGCCGTCGACATTCTGCCTGAAATCCGCGAATGGGAACGCACGTCAGCGGCAGTTCTAAGCG
CTTACATGCTGCCCGGTGTAAGCTCGTATCTCCGGGAACTGGAATCGTCGCTGCAAGAGTCCGGTTACGGCCGCGGGCCG
CTTATCATGCAGATCAATGGCGGCGTTGCATCGGTATCGGAAATCCTGCGCAAGCCGGTCAACGTTCTCGCTTCTGGCCC
TGCGGCGGCTCCTGCTGTCGCAATACACAGCGGCGCTTCCCGCAATATGCAGAACATGGTTATCATCGACATGGGCGGTA
CCAGCCTCGACGTGTGCCTTCTGCGTGGCGGCAGAGCTGCGATGTCGCGTGAGATCAGCGTCGACATGCAGCCGATCGGC
GTGCCAGGGGTCGACGTCCTGTCGGTCGGAGCCGGCGGCGGAAGTATTGCATGGGTCGATAGCGGTGGTGCTCTGCGGGT
TGGGCCGCAAAGTGCGGGCTCTCGACCAGGGCCGGTTGCCTATGATGCGGGTGGTACCGAACCGACAGTTACCGACGCCA
ACATCGTACTGGGTTATCTGGACCCGGAAGCGTTTCTCGGAGGCCGCAGGGTATTGAGAGACGATCTTTCCCAGGCCGCA
CTGGAACGTGTGGGTGGGAAGGTCGGTCTGAACGCTTTGGAGGCAGCGGCGGGCGTCATTCGTATCGTCAATGCCAACAT
GGTTGCCGCTATTCAGGCTGTCTCCGTGCAGCGGGGTATCGACATCAGAAAGTACGCCTTGATGTCTGGTGGTGGCGCGG
GCGGCCTTCATGCCGCTGCGCTTGCACGCGAACTTCGGGTGCCACACGTGATCATTCCGCGCCAAGCGGGCACCCTGTGC
GCATTCGGCATGACGGTGACCGATGTTAGATACGACGAGTCGGGCGTATGCCATACCTATTCCGACGGTCCGGATATTGA
AGGTATCAACCGGATCCTGACGACATTGGCCGAAGGCTTGCGGTCAAAGCTTAAAGCGGAGGGCTTCTCCGACGAGAAGG
TGCGGATCGAGTTTTTCGCGGATGCCCGCTATTCGGGACAGGTTCATCAGCTAACGATCCCTGTTCCCTTTTCTGAAACG
ATCACGGCAACGCAAATTGCGGTAGCCGTCGATAACTTTCACGCGGAACACAAGAGTCAATACACCTACAACCGCCCGAC
CATCCCCGTGGAATTCCTGCATTGGCGCGTTACCGGCATCGGCCTTGCGGGATCGACAACGGTCGTCGGGAAGAATGACG
CATCAACGCCGAACAGGCGCGGTGTCCGCATGGCTTACGATTTCGCCAGCCGCCAAATGGTAGAGACTGCCGTCTATCAC
ATGGACGATCTGACGCCTGGCTCAAGTTTCGTGGGTCCCGCGATCGTCCAGTCCTCCACGACCACCGTCGTCATCGGTGC
TGGCGACAAGCTTTCGGTCGATCCTGACTACGCATTGGCAATTGATGTGAACGTGGACGTCTAA

Upstream 100 bases:

>100_bases
GAAGGCGATGTGTTCCTACTGAATCCAGCTTGATCATGCCGGTATGCCAGCCCCGGTTCGGTGCCGGGCTGGCACGAATT
ACATCAAACAAGGTGACATC

Downstream 100 bases:

>100_bases
CGTCGTTGTCCGGTGGCCGAAGCACCGGAACTTTGCGCTTGATGGACGGTGGCACCCTGCCGCCGTCCATCAAATCTGTT
CGAAGAAGACGTGGTTGGAA

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 687; Mature: 686

Protein sequence:

>687_residues
MSYRIGVDIGGTFTDFSVLDQNGKTFLWKEDSDPVFPAQAIQRGLKAVARELGHTTEEFLKKVSLFVHGTTIATNLLIQR
NGPRIGLLCTEGFRDILYTRDAFKPDRYNLRMPYPEPLVDRYLRLGVRERVNKDGDIITPLDEEGVRQAARSMRDAGVSS
VAVAFLWSIANSEHEYRAAEILREELPNVPVLCAVDILPEIREWERTSAAVLSAYMLPGVSSYLRELESSLQESGYGRGP
LIMQINGGVASVSEILRKPVNVLASGPAAAPAVAIHSGASRNMQNMVIIDMGGTSLDVCLLRGGRAAMSREISVDMQPIG
VPGVDVLSVGAGGGSIAWVDSGGALRVGPQSAGSRPGPVAYDAGGTEPTVTDANIVLGYLDPEAFLGGRRVLRDDLSQAA
LERVGGKVGLNALEAAAGVIRIVNANMVAAIQAVSVQRGIDIRKYALMSGGGAGGLHAAALARELRVPHVIIPRQAGTLC
AFGMTVTDVRYDESGVCHTYSDGPDIEGINRILTTLAEGLRSKLKAEGFSDEKVRIEFFADARYSGQVHQLTIPVPFSET
ITATQIAVAVDNFHAEHKSQYTYNRPTIPVEFLHWRVTGIGLAGSTTVVGKNDASTPNRRGVRMAYDFASRQMVETAVYH
MDDLTPGSSFVGPAIVQSSTTTVVIGAGDKLSVDPDYALAIDVNVDV

Sequences:

>Translated_687_residues
MSYRIGVDIGGTFTDFSVLDQNGKTFLWKEDSDPVFPAQAIQRGLKAVARELGHTTEEFLKKVSLFVHGTTIATNLLIQR
NGPRIGLLCTEGFRDILYTRDAFKPDRYNLRMPYPEPLVDRYLRLGVRERVNKDGDIITPLDEEGVRQAARSMRDAGVSS
VAVAFLWSIANSEHEYRAAEILREELPNVPVLCAVDILPEIREWERTSAAVLSAYMLPGVSSYLRELESSLQESGYGRGP
LIMQINGGVASVSEILRKPVNVLASGPAAAPAVAIHSGASRNMQNMVIIDMGGTSLDVCLLRGGRAAMSREISVDMQPIG
VPGVDVLSVGAGGGSIAWVDSGGALRVGPQSAGSRPGPVAYDAGGTEPTVTDANIVLGYLDPEAFLGGRRVLRDDLSQAA
LERVGGKVGLNALEAAAGVIRIVNANMVAAIQAVSVQRGIDIRKYALMSGGGAGGLHAAALARELRVPHVIIPRQAGTLC
AFGMTVTDVRYDESGVCHTYSDGPDIEGINRILTTLAEGLRSKLKAEGFSDEKVRIEFFADARYSGQVHQLTIPVPFSET
ITATQIAVAVDNFHAEHKSQYTYNRPTIPVEFLHWRVTGIGLAGSTTVVGKNDASTPNRRGVRMAYDFASRQMVETAVYH
MDDLTPGSSFVGPAIVQSSTTTVVIGAGDKLSVDPDYALAIDVNVDV
>Mature_686_residues
SYRIGVDIGGTFTDFSVLDQNGKTFLWKEDSDPVFPAQAIQRGLKAVARELGHTTEEFLKKVSLFVHGTTIATNLLIQRN
GPRIGLLCTEGFRDILYTRDAFKPDRYNLRMPYPEPLVDRYLRLGVRERVNKDGDIITPLDEEGVRQAARSMRDAGVSSV
AVAFLWSIANSEHEYRAAEILREELPNVPVLCAVDILPEIREWERTSAAVLSAYMLPGVSSYLRELESSLQESGYGRGPL
IMQINGGVASVSEILRKPVNVLASGPAAAPAVAIHSGASRNMQNMVIIDMGGTSLDVCLLRGGRAAMSREISVDMQPIGV
PGVDVLSVGAGGGSIAWVDSGGALRVGPQSAGSRPGPVAYDAGGTEPTVTDANIVLGYLDPEAFLGGRRVLRDDLSQAAL
ERVGGKVGLNALEAAAGVIRIVNANMVAAIQAVSVQRGIDIRKYALMSGGGAGGLHAAALARELRVPHVIIPRQAGTLCA
FGMTVTDVRYDESGVCHTYSDGPDIEGINRILTTLAEGLRSKLKAEGFSDEKVRIEFFADARYSGQVHQLTIPVPFSETI
TATQIAVAVDNFHAEHKSQYTYNRPTIPVEFLHWRVTGIGLAGSTTVVGKNDASTPNRRGVRMAYDFASRQMVETAVYHM
DDLTPGSSFVGPAIVQSSTTTVVIGAGDKLSVDPDYALAIDVNVDV

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0145

COG function: function code EQ; N-methylhydantoinase A/acetone carboxylase, beta subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=732, Percent_Identity=29.2349726775956, Blast_Score=207, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI133901900, Length=731, Percent_Identity=25.1709986320109, Blast_Score=181, Evalue=1e-45,
Organism=Saccharomyces cerevisiae, GI6322634, Length=723, Percent_Identity=28.2157676348548, Blast_Score=209, Evalue=1e-54,
Organism=Drosophila melanogaster, GI45550492, Length=726, Percent_Identity=29.4765840220386, Blast_Score=236, Evalue=5e-62,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008040
- InterPro:   IPR002821 [H]

Pfam domain/function: PF05378 Hydant_A_N; PF01968 Hydantoinase_A [H]

EC number: 3.5.2.14

Molecular weight: Translated: 73648; Mature: 73517

Theoretical pI: Translated: 5.68; Mature: 5.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYRIGVDIGGTFTDFSVLDQNGKTFLWKEDSDPVFPAQAIQRGLKAVARELGHTTEEFL
CCEEEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
KKVSLFVHGTTIATNLLIQRNGPRIGLLCTEGFRDILYTRDAFKPDRYNLRMPYPEPLVD
HHHHHHEECHHHEEEEEEEECCCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCHHHH
RYLRLGVRERVNKDGDIITPLDEEGVRQAARSMRDAGVSSVAVAFLWSIANSEHEYRAAE
HHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
ILREELPNVPVLCAVDILPEIREWERTSAAVLSAYMLPGVSSYLRELESSLQESGYGRGP
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
LIMQINGGVASVSEILRKPVNVLASGPAAAPAVAIHSGASRNMQNMVIIDMGGTSLDVCL
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEE
LRGGRAAMSREISVDMQPIGVPGVDVLSVGAGGGSIAWVDSGGALRVGPQSAGSRPGPVA
ECCCHHHHCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCEE
YDAGGTEPTVTDANIVLGYLDPEAFLGGRRVLRDDLSQAALERVGGKVGLNALEAAAGVI
ECCCCCCCCEECCEEEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEE
RIVNANMVAAIQAVSVQRGIDIRKYALMSGGGAGGLHAAALARELRVPHVIIPRQAGTLC
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
AFGMTVTDVRYDESGVCHTYSDGPDIEGINRILTTLAEGLRSKLKAEGFSDEKVRIEFFA
EECCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DARYSGQVHQLTIPVPFSETITATQIAVAVDNFHAEHKSQYTYNRPTIPVEFLHWRVTGI
CCCCCCCEEEEEEECCCCCCCCCEEEEEEEHHHHHHHCCCEEECCCCCEEEEEEEEEEEE
GLAGSTTVVGKNDASTPNRRGVRMAYDFASRQMVETAVYHMDDLTPGSSFVGPAIVQSST
EECCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
TTVVIGAGDKLSVDPDYALAIDVNVDV
CEEEEECCCEEECCCCEEEEEEEEECC
>Mature Secondary Structure 
SYRIGVDIGGTFTDFSVLDQNGKTFLWKEDSDPVFPAQAIQRGLKAVARELGHTTEEFL
CEEEEEECCCCCCCCEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCHHHHH
KKVSLFVHGTTIATNLLIQRNGPRIGLLCTEGFRDILYTRDAFKPDRYNLRMPYPEPLVD
HHHHHHEECHHHEEEEEEEECCCEEEEEECCCHHHHHHHCCCCCCCCEEEECCCCCHHHH
RYLRLGVRERVNKDGDIITPLDEEGVRQAARSMRDAGVSSVAVAFLWSIANSEHEYRAAE
HHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHH
ILREELPNVPVLCAVDILPEIREWERTSAAVLSAYMLPGVSSYLRELESSLQESGYGRGP
HHHHHCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCC
LIMQINGGVASVSEILRKPVNVLASGPAAAPAVAIHSGASRNMQNMVIIDMGGTSLDVCL
EEEEECCCHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCEEEEEEECCCEEEEEE
LRGGRAAMSREISVDMQPIGVPGVDVLSVGAGGGSIAWVDSGGALRVGPQSAGSRPGPVA
ECCCHHHHCCEEEECCCCCCCCCCCEEEECCCCCEEEEEECCCEEEECCCCCCCCCCCEE
YDAGGTEPTVTDANIVLGYLDPEAFLGGRRVLRDDLSQAALERVGGKVGLNALEAAAGVI
ECCCCCCCCEECCEEEEEEECHHHHHCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCEE
RIVNANMVAAIQAVSVQRGIDIRKYALMSGGGAGGLHAAALARELRVPHVIIPRQAGTLC
EEECCCHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCEEEECCCCCCEE
AFGMTVTDVRYDESGVCHTYSDGPDIEGINRILTTLAEGLRSKLKAEGFSDEKVRIEFFA
EECCEEEEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEE
DARYSGQVHQLTIPVPFSETITATQIAVAVDNFHAEHKSQYTYNRPTIPVEFLHWRVTGI
CCCCCCCEEEEEEECCCCCCCCCEEEEEEEHHHHHHHCCCEEECCCCCEEEEEEEEEEEE
GLAGSTTVVGKNDASTPNRRGVRMAYDFASRQMVETAVYHMDDLTPGSSFVGPAIVQSST
EECCCEEEEECCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCHHCCCCC
TTVVIGAGDKLSVDPDYALAIDVNVDV
CEEEEECCCEEECCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]