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The map label for this gene is hyuB [H]

Identifier: 209552122

GI number: 209552122

Start: 218788

End: 220692

Strand: Reverse

Name: hyuB [H]

Synonym: Rleg2_6273

Alternate gene names: 209552122

Gene position: 220692-218788 (Counterclockwise)

Preceding gene: 209552123

Following gene: 209552121

Centisome position: 71.48

GC content: 58.74

Gene sequence:

>1905_bases
ATGTTTAATACGGATTACGGTGCAAATGCATCCGACAAACACTCGAAAACGGAAGGGCCAGCCGTTGCCGGTGTCGATCC
CATAACCTTCAGCATTTTGTTGAGCCGTTTCAACAGTATCATCGAAGAGATGTCCGCAACGCTGGAGCGCAGCGCCCAGA
GCACGATGTACTCGCTATGCCGCGATTTCGATTGCGTGGTTCTCGACGCACAGGCCAGACAGATCGCATGCGGCGACGCG
CTTCCCGTGCACACGCTGACTACCCATCTCGTTGTCCTCGAAATCATCAAGGCGTTCGATGGCGACATCAATGACGGCGA
TGTTTTTCTTTGCAATTCGTCGTTCCACGGGAACACCCATCCCGCGGACGTCGCGACCTGCACTCCCGTGTTCTACGACG
GTAAACTCGTTTTCTGGTCGACGGCCCGCGGTCATCAGCTGGATATCGGCGCGTTCCTTCCATCAAGCGTTCTGCCGGCC
GCGCAGAATGTCTATCAAGAAGGCATCACCATCCCGCCGCTCAAGCTGGTCGACCAAGGACGGATGAGAAAAGACGTGCG
GGAACTGTATTTCTGGAACGTTCGCTACCGAGATTTGCTGGAAGGCGACATGCTCGCGCAGCTCGGATCGATCGAGAAGG
GCAAGCAGCGTCTGCTTGAGATCTGCGCTGAATACGGTGTCGCGACCGCGGAAAGTTGCATCGATGCCCTCATCACCTAT
TCCAATAGGCGTGCAAAGGCCGAATTCGCAAAAATCCCGGATGGTGTCTATCACGGCCAAGGTTGGATCGACAGCGATGG
CGTCGACGTTCTCGATATTCCGATCAACGTCAGCGTGCAGATCAAAAATGGTGACGTCGTCGTCGATTACGAGGGCAGCG
GCCCGCAGGCGCGCGGTGGCGTGAACGGTTCGATCGCAACCTCTAAAGCGGCGGGCGCTGCGCCCTTCATGTGCTACCTC
CCCACCGATATTCCGCATAACCAGGGGAAGATCGATGCGGTTACCGTGAAGCTGCCCGAAGGAACTATTGTCAACCCCCA
TTTCCCTGCATCGACGTCGTGCGCCACCATCATCCCCTCGGATATGATGTCCGATGCCGTGCACAAGGCGCTTTCAGACG
TCTTGCCGGACCGCGTCATGGGAGGAAGCACCCGTTGCGCCAACGTTCCCGGTTTTGCGGGAGAAAAAGACTGGGCGGGG
CGGCCATGGGGTGTGATGCTGTTTAACAACACGGGAGGATTCGGAGCTGCTGCCGATAACGACGGCTGGCCCCTTTGCGA
AAGCGTGGCGGCTCTCGGTGGGATGAGAGCCCAGCCGCTGGAGCAGCTTGAAATGCTCCATCCTCTGCTCGTCGATTTCC
TCGAAATCGAAACGGACTCCATGGGATACGGCAAGCATATCGGCGGACCCGGATTAAGCATCGGCCTCCGTCCGCTGAAC
GGGACGATTGATTGCTTCTCCTTCGGCGACGGCTGGCGCAATCCGCCGCACGGCGTATCGCAGGGTACGGCATCGCCAGG
CGGCGGTCAGTACGTGGAGAATCTCAATACCGACCAGCGCAGGTATTTCTCCGCGACCGGAAACGTCAAGGTGGCGATGG
ATGAGCGGTATGTCGGCATCTCCTGCGGCGGCGGCGGGCATGGCAACCCGATGGACCGCGACATTGAGCTTGTCCGCAAG
AACGTCCGTGATGGACTGATCAGCCGCGAGGTTGCGGCGAGCACGTTCGGTGTCATTCTGGATGACGGTCTGGATCCCAA
AATCCTCGAAAAGGAGACCGCCGCCAGACGTCTGGAACTTGGCAGGTCCACCCGGCCTTCCGTCGAGCCGGTCCAGCCGG
GCACCGGTCGCTGGGCGGAGAACCATATGCGCGAAGGCGATGTGTTCCTACTGAATCCAGCTTGA

Upstream 100 bases:

>100_bases
TCAGGGACCGGGGCGGGTGCCGCGCTCCCTGAGAAACGGGATCGCCAGACGGATAGCTCGGCGGTGGTCTACCGGACGAT
GTTAAAGAAGGAGAACCAGC

Downstream 100 bases:

>100_bases
TCATGCCGGTATGCCAGCCCCGGTTCGGTGCCGGGCTGGCACGAATTACATCAAACAAGGTGACATCATGAGCTATCGCA
TCGGCGTCGATATTGGCGGC

Product: 5-oxoprolinase (ATP-hydrolyzing)

Products: ADP; phosphate; N-carbamoylsarcosine

Alternate protein names: ORF3 [H]

Number of amino acids: Translated: 634; Mature: 634

Protein sequence:

>634_residues
MFNTDYGANASDKHSKTEGPAVAGVDPITFSILLSRFNSIIEEMSATLERSAQSTMYSLCRDFDCVVLDAQARQIACGDA
LPVHTLTTHLVVLEIIKAFDGDINDGDVFLCNSSFHGNTHPADVATCTPVFYDGKLVFWSTARGHQLDIGAFLPSSVLPA
AQNVYQEGITIPPLKLVDQGRMRKDVRELYFWNVRYRDLLEGDMLAQLGSIEKGKQRLLEICAEYGVATAESCIDALITY
SNRRAKAEFAKIPDGVYHGQGWIDSDGVDVLDIPINVSVQIKNGDVVVDYEGSGPQARGGVNGSIATSKAAGAAPFMCYL
PTDIPHNQGKIDAVTVKLPEGTIVNPHFPASTSCATIIPSDMMSDAVHKALSDVLPDRVMGGSTRCANVPGFAGEKDWAG
RPWGVMLFNNTGGFGAAADNDGWPLCESVAALGGMRAQPLEQLEMLHPLLVDFLEIETDSMGYGKHIGGPGLSIGLRPLN
GTIDCFSFGDGWRNPPHGVSQGTASPGGGQYVENLNTDQRRYFSATGNVKVAMDERYVGISCGGGGHGNPMDRDIELVRK
NVRDGLISREVAASTFGVILDDGLDPKILEKETAARRLELGRSTRPSVEPVQPGTGRWAENHMREGDVFLLNPA

Sequences:

>Translated_634_residues
MFNTDYGANASDKHSKTEGPAVAGVDPITFSILLSRFNSIIEEMSATLERSAQSTMYSLCRDFDCVVLDAQARQIACGDA
LPVHTLTTHLVVLEIIKAFDGDINDGDVFLCNSSFHGNTHPADVATCTPVFYDGKLVFWSTARGHQLDIGAFLPSSVLPA
AQNVYQEGITIPPLKLVDQGRMRKDVRELYFWNVRYRDLLEGDMLAQLGSIEKGKQRLLEICAEYGVATAESCIDALITY
SNRRAKAEFAKIPDGVYHGQGWIDSDGVDVLDIPINVSVQIKNGDVVVDYEGSGPQARGGVNGSIATSKAAGAAPFMCYL
PTDIPHNQGKIDAVTVKLPEGTIVNPHFPASTSCATIIPSDMMSDAVHKALSDVLPDRVMGGSTRCANVPGFAGEKDWAG
RPWGVMLFNNTGGFGAAADNDGWPLCESVAALGGMRAQPLEQLEMLHPLLVDFLEIETDSMGYGKHIGGPGLSIGLRPLN
GTIDCFSFGDGWRNPPHGVSQGTASPGGGQYVENLNTDQRRYFSATGNVKVAMDERYVGISCGGGGHGNPMDRDIELVRK
NVRDGLISREVAASTFGVILDDGLDPKILEKETAARRLELGRSTRPSVEPVQPGTGRWAENHMREGDVFLLNPA
>Mature_634_residues
MFNTDYGANASDKHSKTEGPAVAGVDPITFSILLSRFNSIIEEMSATLERSAQSTMYSLCRDFDCVVLDAQARQIACGDA
LPVHTLTTHLVVLEIIKAFDGDINDGDVFLCNSSFHGNTHPADVATCTPVFYDGKLVFWSTARGHQLDIGAFLPSSVLPA
AQNVYQEGITIPPLKLVDQGRMRKDVRELYFWNVRYRDLLEGDMLAQLGSIEKGKQRLLEICAEYGVATAESCIDALITY
SNRRAKAEFAKIPDGVYHGQGWIDSDGVDVLDIPINVSVQIKNGDVVVDYEGSGPQARGGVNGSIATSKAAGAAPFMCYL
PTDIPHNQGKIDAVTVKLPEGTIVNPHFPASTSCATIIPSDMMSDAVHKALSDVLPDRVMGGSTRCANVPGFAGEKDWAG
RPWGVMLFNNTGGFGAAADNDGWPLCESVAALGGMRAQPLEQLEMLHPLLVDFLEIETDSMGYGKHIGGPGLSIGLRPLN
GTIDCFSFGDGWRNPPHGVSQGTASPGGGQYVENLNTDQRRYFSATGNVKVAMDERYVGISCGGGGHGNPMDRDIELVRK
NVRDGLISREVAASTFGVILDDGLDPKILEKETAARRLELGRSTRPSVEPVQPGTGRWAENHMREGDVFLLNPA

Specific function: HyuA and hyuB convert D- and L-substituted hydantoins to corresponding N-carbamyl-amino acids [H]

COG id: COG0146

COG function: function code EQ; N-methylhydantoinase B/acetone carboxylase, alpha subunit

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the oxoprolinase family [H]

Homologues:

Organism=Homo sapiens, GI48314820, Length=363, Percent_Identity=29.2011019283747, Blast_Score=138, Evalue=1e-32,
Organism=Caenorhabditis elegans, GI133901900, Length=396, Percent_Identity=27.5252525252525, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI133901902, Length=359, Percent_Identity=25.9052924791086, Blast_Score=128, Evalue=1e-29,
Organism=Saccharomyces cerevisiae, GI6322634, Length=549, Percent_Identity=25.8652094717668, Blast_Score=169, Evalue=1e-42,
Organism=Drosophila melanogaster, GI45550492, Length=547, Percent_Identity=24.3144424131627, Blast_Score=132, Evalue=1e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003692 [H]

Pfam domain/function: PF02538 Hydantoinase_B [H]

EC number: 3.5.2.14

Molecular weight: Translated: 68206; Mature: 68206

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFNTDYGANASDKHSKTEGPAVAGVDPITFSILLSRFNSIIEEMSATLERSAQSTMYSLC
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDFDCVVLDAQARQIACGDALPVHTLTTHLVVLEIIKAFDGDINDGDVFLCNSSFHGNTH
CCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
PADVATCTPVFYDGKLVFWSTARGHQLDIGAFLPSSVLPAAQNVYQEGITIPPLKLVDQG
CCCCEEECEEEECCEEEEEECCCCCEEEEHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCH
RMRKDVRELYFWNVRYRDLLEGDMLAQLGSIEKGKQRLLEICAEYGVATAESCIDALITY
HHHHHHHHHHEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SNRRAKAEFAKIPDGVYHGQGWIDSDGVDVLDIPINVSVQIKNGDVVVDYEGSGPQARGG
CCCHHHHHHHHCCCCEECCCCEECCCCCEEEEECEEEEEEEECCCEEEEECCCCCCCCCC
VNGSIATSKAAGAAPFMCYLPTDIPHNQGKIDAVTVKLPEGTIVNPHFPASTSCATIIPS
CCCCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEECCH
DMMSDAVHKALSDVLPDRVMGGSTRCANVPGFAGEKDWAGRPWGVMLFNNTGGFGAAADN
HHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
DGWPLCESVAALGGMRAQPLEQLEMLHPLLVDFLEIETDSMGYGKHIGGPGLSIGLRPLN
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
GTIDCFSFGDGWRNPPHGVSQGTASPGGGQYVENLNTDQRRYFSATGNVKVAMDERYVGI
CCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHEEEECCCCEEEEEECEEEEE
SCGGGGHGNPMDRDIELVRKNVRDGLISREVAASTFGVILDDGLDPKILEKETAARRLEL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHC
GRSTRPSVEPVQPGTGRWAENHMREGDVFLLNPA
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCC
>Mature Secondary Structure
MFNTDYGANASDKHSKTEGPAVAGVDPITFSILLSRFNSIIEEMSATLERSAQSTMYSLC
CCCCCCCCCCCCCCCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RDFDCVVLDAQARQIACGDALPVHTLTTHLVVLEIIKAFDGDINDGDVFLCNSSFHGNTH
CCCCEEEECCCCCEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCC
PADVATCTPVFYDGKLVFWSTARGHQLDIGAFLPSSVLPAAQNVYQEGITIPPLKLVDQG
CCCCEEECEEEECCEEEEEECCCCCEEEEHHHCCHHHHHHHHHHHHCCCCCCCHHHHCCH
RMRKDVRELYFWNVRYRDLLEGDMLAQLGSIEKGKQRLLEICAEYGVATAESCIDALITY
HHHHHHHHHHEECCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SNRRAKAEFAKIPDGVYHGQGWIDSDGVDVLDIPINVSVQIKNGDVVVDYEGSGPQARGG
CCCHHHHHHHHCCCCEECCCCEECCCCCEEEEECEEEEEEEECCCEEEEECCCCCCCCCC
VNGSIATSKAAGAAPFMCYLPTDIPHNQGKIDAVTVKLPEGTIVNPHFPASTSCATIIPS
CCCCEECCCCCCCCCEEEEECCCCCCCCCCEEEEEEECCCCCEECCCCCCCCCCCEECCH
DMMSDAVHKALSDVLPDRVMGGSTRCANVPGFAGEKDWAGRPWGVMLFNNTGGFGAAADN
HHHHHHHHHHHHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
DGWPLCESVAALGGMRAQPLEQLEMLHPLLVDFLEIETDSMGYGKHIGGPGLSIGLRPLN
CCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEEECC
GTIDCFSFGDGWRNPPHGVSQGTASPGGGQYVENLNTDQRRYFSATGNVKVAMDERYVGI
CCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCHHEEEECCCCEEEEEECEEEEE
SCGGGGHGNPMDRDIELVRKNVRDGLISREVAASTFGVILDDGLDPKILEKETAARRLEL
EECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCEEEECCCCHHHHHHHHHHHHHHC
GRSTRPSVEPVQPGTGRWAENHMREGDVFLLNPA
CCCCCCCCCCCCCCCCCHHHHHCCCCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; N-methylimidazolidine-2,4-dione; H2O

Specific reaction: ATP + N-methylimidazolidine-2,4-dione + 2 H2O = ADP + phosphate + N-carbamoylsarcosine

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732229 [H]