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The map label for this gene is yeiT [H]

Identifier: 209550604

GI number: 209550604

Start: 3086637

End: 3087998

Strand: Direct

Name: yeiT [H]

Synonym: Rleg2_3028

Alternate gene names: 209550604

Gene position: 3086637-3087998 (Clockwise)

Preceding gene: 209550603

Following gene: 209550605

Centisome position: 68.02

GC content: 64.39

Gene sequence:

>1362_bases
ATGGAACGACTGGAAACTGGGATTCATGCCGGCCGGCTTTCGCCCGCCGAGTATGAGGCTAATTTTTCCGATCTTCATCC
GCGCCTCGACAATCACGAGGCGCTGGTCGCCGCCGACCGCTGTTATTTCTGTTATGACGCGCCGTGCATGACGGCCTGTC
CCACCTCGATCGACATTCCGCTGTTCATCCGCCAGATTTCGACCGGCAATCCGATCGGCTCGGCCAAGACCATCTTCGAC
CAGAACATCCTCGGCGGCATGTGCGCCCGCGTCTGTCCCACCGAAGAGCTCTGCGAACAGGCCTGTGTGCGCAACACGGC
CGAAGAACGCCCTGTCGAGATCGGCCGCCTGCAGCGTTATGCGACCGATACCGCCATGCAGGCCGGCAGGCAGTTCTACG
CCAGGGCCGAAGCATCAGGCAAGACGATCGCCGTCGTCGGCGCAGGCCCGGCCGGCCTTGCCGCCGCCCATCGTCTGGCC
GTGAAGGGCCATTCCGTCGTCATCTATGATGCCAGGGAAAAATCCGGCGGCCTCAACGAATACGGCATCGCCACCTACAA
GACGGTCGACGATTTCGCCCAGGCGGAAGTCGACTATGTGCTTTCGATCGGCGGCATCGAGGTTCGCCACGGCGCTCTTC
TCGGCCGCGATTTCTCGCTTGCCGATCTGCAGGCGCAATATGACGCCGTCTTCATCGGCATCGGCCTTGCCGGCGTCAAT
GCGCTACGCATCGACGGCGAGAATCTCGCCGGCGTCGACGACGCCGTCGATTTCATCGCGGCACTGCGCCAGGCCGAAGA
CAAGAGCCAGATCGCCATCGGCCGCCGTGTCGTCGTCCTCGGCGGCGGCATGACGGCGATCGACGCTGCCGTCCAGGCAA
AGCTGCTCGGCGCCGAGGAGGTGACGATCTGTTACCGCCGCGGCAAGGAGCACATGAACGCCTCGGAATATGAGCAGGAT
CTGGCAAGCTCCAAGGGTGTTATCATCCGCCACTGGCTGGCGCCGAAATCGATCCTGTCGCAGGACGGCAAGGTCGCCGC
CATCGAGGTGGAATACACGCGGATCCTCGAAGGCCGCCTGATCGGCACCGGCGAAACCGGCGTAATCGCCGCCGACCAGA
TCTTCAAGGCGATCGGCCAGAGCTTTGACGCCTCGGGCCTCGGTTCGTTGCGCATGGAATCCGGCCGCATTGCTGTCGAT
GCCGACGGCCACACCTCGCTCGACGGCGTCTGGGCCGGCGGCGACTGCGTCTTCGGCGGCGACGATCTCACGGTTTCCGC
CGTCGCTCACGGCCGCGACGCGGCTGAATCCATCCATCGCACCCTCACCGCAGCCGCCGCTCCGGCTGTCGCGGTTGCTT
GA

Upstream 100 bases:

>100_bases
TTCCCAAGGCAGGTTCGTCAAAATTTTACCGTTTGATAAATTTTTAAACCTGAGTTACGCTTTCCCTTACAGGCCGTTTA
CCAATAATGAGGGAACTTCA

Downstream 100 bases:

>100_bases
AGGGGAGAATGAATCATGGCTGATCTCCGCAATAATTTCGTCGGCATCAAGTCCCCGAACCCGTTCTGGCTGGCCTCTGC
GCCGCCGACCGACAAGGCCT

Product: oxidoreductase

Products: L-glutamine; 2-oxoglutarate; NADPH; H+

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MERLETGIHAGRLSPAEYEANFSDLHPRLDNHEALVAADRCYFCYDAPCMTACPTSIDIPLFIRQISTGNPIGSAKTIFD
QNILGGMCARVCPTEELCEQACVRNTAEERPVEIGRLQRYATDTAMQAGRQFYARAEASGKTIAVVGAGPAGLAAAHRLA
VKGHSVVIYDAREKSGGLNEYGIATYKTVDDFAQAEVDYVLSIGGIEVRHGALLGRDFSLADLQAQYDAVFIGIGLAGVN
ALRIDGENLAGVDDAVDFIAALRQAEDKSQIAIGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASEYEQD
LASSKGVIIRHWLAPKSILSQDGKVAAIEVEYTRILEGRLIGTGETGVIAADQIFKAIGQSFDASGLGSLRMESGRIAVD
ADGHTSLDGVWAGGDCVFGGDDLTVSAVAHGRDAAESIHRTLTAAAAPAVAVA

Sequences:

>Translated_453_residues
MERLETGIHAGRLSPAEYEANFSDLHPRLDNHEALVAADRCYFCYDAPCMTACPTSIDIPLFIRQISTGNPIGSAKTIFD
QNILGGMCARVCPTEELCEQACVRNTAEERPVEIGRLQRYATDTAMQAGRQFYARAEASGKTIAVVGAGPAGLAAAHRLA
VKGHSVVIYDAREKSGGLNEYGIATYKTVDDFAQAEVDYVLSIGGIEVRHGALLGRDFSLADLQAQYDAVFIGIGLAGVN
ALRIDGENLAGVDDAVDFIAALRQAEDKSQIAIGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASEYEQD
LASSKGVIIRHWLAPKSILSQDGKVAAIEVEYTRILEGRLIGTGETGVIAADQIFKAIGQSFDASGLGSLRMESGRIAVD
ADGHTSLDGVWAGGDCVFGGDDLTVSAVAHGRDAAESIHRTLTAAAAPAVAVA
>Mature_453_residues
MERLETGIHAGRLSPAEYEANFSDLHPRLDNHEALVAADRCYFCYDAPCMTACPTSIDIPLFIRQISTGNPIGSAKTIFD
QNILGGMCARVCPTEELCEQACVRNTAEERPVEIGRLQRYATDTAMQAGRQFYARAEASGKTIAVVGAGPAGLAAAHRLA
VKGHSVVIYDAREKSGGLNEYGIATYKTVDDFAQAEVDYVLSIGGIEVRHGALLGRDFSLADLQAQYDAVFIGIGLAGVN
ALRIDGENLAGVDDAVDFIAALRQAEDKSQIAIGRRVVVLGGGMTAIDAAVQAKLLGAEEVTICYRRGKEHMNASEYEQD
LASSKGVIIRHWLAPKSILSQDGKVAAIEVEYTRILEGRLIGTGETGVIAADQIFKAIGQSFDASGLGSLRMESGRIAVD
ADGHTSLDGVWAGGDCVFGGDDLTVSAVAHGRDAAESIHRTLTAAAAPAVAVA

Specific function: Unknown

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To E.coli glutamate synthase [NADPH] small chain (GltD) and to E.coli AegA [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=453, Percent_Identity=31.7880794701987, Blast_Score=176, Evalue=3e-44,
Organism=Homo sapiens, GI237757300, Length=113, Percent_Identity=46.0176991150442, Blast_Score=96, Evalue=5e-20,
Organism=Escherichia coli, GI1788468, Length=431, Percent_Identity=34.5707656612529, Blast_Score=212, Evalue=3e-56,
Organism=Escherichia coli, GI87082180, Length=459, Percent_Identity=32.8976034858388, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789606, Length=446, Percent_Identity=32.9596412556054, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1788811, Length=446, Percent_Identity=33.4080717488789, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1789243, Length=432, Percent_Identity=28.7037037037037, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71984108, Length=461, Percent_Identity=33.1887201735358, Blast_Score=174, Evalue=9e-44,
Organism=Caenorhabditis elegans, GI17570289, Length=455, Percent_Identity=25.9340659340659, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6320030, Length=477, Percent_Identity=26.4150943396226, Blast_Score=115, Evalue=2e-26,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=32.6086956521739, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=32.6086956521739, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI28574881, Length=446, Percent_Identity=28.0269058295964, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24665539, Length=446, Percent_Identity=28.0269058295964, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24665547, Length=461, Percent_Identity=27.765726681128, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24665543, Length=461, Percent_Identity=27.765726681128, Blast_Score=125, Evalue=6e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR016040
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: 1.4.1.13

Molecular weight: Translated: 47884; Mature: 47884

Theoretical pI: Translated: 4.81; Mature: 4.81

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MERLETGIHAGRLSPAEYEANFSDLHPRLDNHEALVAADRCYFCYDAPCMTACPTSIDIP
CCHHHHCCCCCCCCCHHHCCCHHHCCCCCCCCCEEEEECCEEEEECCCCHHCCCCCCCCC
LFIRQISTGNPIGSAKTIFDQNILGGMCARVCPTEELCEQACVRNTAEERPVEIGRLQRY
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHH
ATDTAMQAGRQFYARAEASGKTIAVVGAGPAGLAAAHRLAVKGHSVVIYDAREKSGGLNE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHEEECCCEEEEEECCCCCCCCCC
YGIATYKTVDDFAQAEVDYVLSIGGIEVRHGALLGRDFSLADLQAQYDAVFIGIGLAGVN
CCCEEEEHHHHHHHHCEEEEEEECCEEEECCEEECCCCCHHHHHHCCCEEEEEEEECCCE
ALRIDGENLAGVDDAVDFIAALRQAEDKSQIAIGRRVVVLGGGMTAIDAAVQAKLLGAEE
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCHHHHHHHHHHHHCCCCH
VTICYRRGKEHMNASEYEQDLASSKGVIIRHWLAPKSILSQDGKVAAIEVEYTRILEGRL
HHHHHHCCHHHCCHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCEEEEEEEEEEEECCEE
IGTGETGVIAADQIFKAIGQSFDASGLGSLRMESGRIAVDADGHTSLDGVWAGGDCVFGG
EECCCCCEEEHHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCEEEECCEEEECC
DDLTVSAVAHGRDAAESIHRTLTAAAAPAVAVA
CCEEEEEECCCCHHHHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MERLETGIHAGRLSPAEYEANFSDLHPRLDNHEALVAADRCYFCYDAPCMTACPTSIDIP
CCHHHHCCCCCCCCCHHHCCCHHHCCCCCCCCCEEEEECCEEEEECCCCHHCCCCCCCCC
LFIRQISTGNPIGSAKTIFDQNILGGMCARVCPTEELCEQACVRNTAEERPVEIGRLQRY
EEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHCCHHHHHHHHH
ATDTAMQAGRQFYARAEASGKTIAVVGAGPAGLAAAHRLAVKGHSVVIYDAREKSGGLNE
HHHHHHHHHHHHHHHHCCCCCEEEEEECCCHHHHHHHHEEECCCEEEEEECCCCCCCCCC
YGIATYKTVDDFAQAEVDYVLSIGGIEVRHGALLGRDFSLADLQAQYDAVFIGIGLAGVN
CCCEEEEHHHHHHHHCEEEEEEECCEEEECCEEECCCCCHHHHHHCCCEEEEEEEECCCE
ALRIDGENLAGVDDAVDFIAALRQAEDKSQIAIGRRVVVLGGGMTAIDAAVQAKLLGAEE
EEEECCCCCCCHHHHHHHHHHHHHCCCCCCEEECCEEEEEECCHHHHHHHHHHHHCCCCH
VTICYRRGKEHMNASEYEQDLASSKGVIIRHWLAPKSILSQDGKVAAIEVEYTRILEGRL
HHHHHHCCHHHCCHHHHHHHHHCCCCEEEEEECCCHHHHCCCCCEEEEEEEEEEEECCEE
IGTGETGVIAADQIFKAIGQSFDASGLGSLRMESGRIAVDADGHTSLDGVWAGGDCVFGG
EECCCCCEEEHHHHHHHHCCCCCCCCCCCEEEECCCEEEECCCCCCCCEEEECCEEEECC
DDLTVSAVAHGRDAAESIHRTLTAAAAPAVAVA
CCEEEEEECCCCHHHHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: L-glutamate; NADP+

Specific reaction: 2 L-glutamate + NADP+ = L-glutamine + 2-oxoglutarate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]