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The map label for this gene is oppB [H]

Identifier: 209550414

GI number: 209550414

Start: 2885819

End: 2886742

Strand: Direct

Name: oppB [H]

Synonym: Rleg2_2835

Alternate gene names: 209550414

Gene position: 2885819-2886742 (Clockwise)

Preceding gene: 209550413

Following gene: 209550415

Centisome position: 63.59

GC content: 60.06

Gene sequence:

>924_bases
ATGATCAAATACGCCCTCCGTCGTTTGCTATCGACGATCCCGGTTCTGTGGATCGCCGTCACAGCCTGTTTTTTCGTTTT
GCGCCTTGCCCCCGGCGGCCCTTTCGATGGCGAAAGGCCATTGCCGCCGGTGATCCTGAAAAACCTTGCCGTCCACTACA
ATCTCGATAAGCCGCTGATCCAGCAGTATCTGATCTATGTCGGCGACCTGCTGCGGGGCGATCTCGGCCCCTCCTTTGCG
AGTGAGGATTTCACCGTCGCCCAGCAGATCATGATCGGCCTGCCCTACACCTTCACCATCGGCACGGCTGCCTTTCTGAT
TGCCATCATCGTCGGCGTGGCCGTCGGCTGTCTTGGGGCGCTCTACCAGAACAAGGCTCCGGATTACATTCTGGGCGCCC
TCATCCTGGTGGGCGTCGTGCTGCCGAATTTCCTGATTGCGCCGATCCTGCAGCTTATCTTCGGCATCCATCTCGCCTGG
TTTCCGGTCGGCGGCTGGGGCGACGGCTCGATCAAATACCTGATCCTGCCGATCGTCGTTCTGTCCCTGCCGCATGCAGG
CCGCATCTCGCGCATCACCCGTGGCTCGATGATCGAGGTGATGAACCAGAACTTCATCCGCACGGCGAAGGCCAAGGGCA
TCGGCCCGCGGCTGACGGTGATGCGCCATGCGCTGAAGCCGGCGCTGATGCCGGTCGTCTCCTATCTGGGTCCGGCGGCA
AGCTACCTTCTCACCGGCTCGCTGGTCGTCGAGAGCATCTTCGGATTGCCCGGCATCGGCCGCTACTTCGTCAACGCGGC
GCTGAACCGCGATTACGGCATGGTTCTCGGTACGGTCATCTTCTACATGGTGTTGATCGTGTTCCTGAACCTTCTGGTCG
ATATCGCCTATGCCTGGCTGGATCCGAAAGTGAGAAACCGATGA

Upstream 100 bases:

>100_bases
CGATAACATCCAGGACATCCACCGCACCCGCTGGCTGTCGATGAAAGAGTAAGGGAAAACGGTCCTTGCCGCGCTCCGCG
GCAAGGACCGGCCCACGACC

Downstream 100 bases:

>100_bases
TCCTTAACCCCGCAAAACGCGAACTTCTCGCCCAGGAGCTTCTGGAGGCGGAAGGCCTTGCACCTGAAGGCCGCTCGCTC
ACCAGGGATGCGCTGCGCCG

Product: binding-protein-dependent transporters inner membrane component

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MIKYALRRLLSTIPVLWIAVTACFFVLRLAPGGPFDGERPLPPVILKNLAVHYNLDKPLIQQYLIYVGDLLRGDLGPSFA
SEDFTVAQQIMIGLPYTFTIGTAAFLIAIIVGVAVGCLGALYQNKAPDYILGALILVGVVLPNFLIAPILQLIFGIHLAW
FPVGGWGDGSIKYLILPIVVLSLPHAGRISRITRGSMIEVMNQNFIRTAKAKGIGPRLTVMRHALKPALMPVVSYLGPAA
SYLLTGSLVVESIFGLPGIGRYFVNAALNRDYGMVLGTVIFYMVLIVFLNLLVDIAYAWLDPKVRNR

Sequences:

>Translated_307_residues
MIKYALRRLLSTIPVLWIAVTACFFVLRLAPGGPFDGERPLPPVILKNLAVHYNLDKPLIQQYLIYVGDLLRGDLGPSFA
SEDFTVAQQIMIGLPYTFTIGTAAFLIAIIVGVAVGCLGALYQNKAPDYILGALILVGVVLPNFLIAPILQLIFGIHLAW
FPVGGWGDGSIKYLILPIVVLSLPHAGRISRITRGSMIEVMNQNFIRTAKAKGIGPRLTVMRHALKPALMPVVSYLGPAA
SYLLTGSLVVESIFGLPGIGRYFVNAALNRDYGMVLGTVIFYMVLIVFLNLLVDIAYAWLDPKVRNR
>Mature_307_residues
MIKYALRRLLSTIPVLWIAVTACFFVLRLAPGGPFDGERPLPPVILKNLAVHYNLDKPLIQQYLIYVGDLLRGDLGPSFA
SEDFTVAQQIMIGLPYTFTIGTAAFLIAIIVGVAVGCLGALYQNKAPDYILGALILVGVVLPNFLIAPILQLIFGIHLAW
FPVGGWGDGSIKYLILPIVVLSLPHAGRISRITRGSMIEVMNQNFIRTAKAKGIGPRLTVMRHALKPALMPVVSYLGPAA
SYLLTGSLVVESIFGLPGIGRYFVNAALNRDYGMVLGTVIFYMVLIVFLNLLVDIAYAWLDPKVRNR

Specific function: Part of the binding-protein-dependent transport system for oligopeptides; probably responsible for the translocation of the substrate across the membrane [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787497, Length=305, Percent_Identity=50.8196721311475, Blast_Score=326, Evalue=9e-91,
Organism=Escherichia coli, GI1787053, Length=310, Percent_Identity=31.6129032258064, Blast_Score=175, Evalue=3e-45,
Organism=Escherichia coli, GI1789888, Length=312, Percent_Identity=33.0128205128205, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1789965, Length=339, Percent_Identity=32.1533923303835, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788504, Length=362, Percent_Identity=27.9005524861878, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1787550, Length=273, Percent_Identity=28.9377289377289, Blast_Score=124, Evalue=9e-30,
Organism=Escherichia coli, GI1787761, Length=312, Percent_Identity=30.4487179487179, Blast_Score=121, Evalue=5e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 33533; Mature: 33533

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKYALRRLLSTIPVLWIAVTACFFVLRLAPGGPFDGERPLPPVILKNLAVHYNLDKPLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
QQYLIYVGDLLRGDLGPSFASEDFTVAQQIMIGLPYTFTIGTAAFLIAIIVGVAVGCLGA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYQNKAPDYILGALILVGVVLPNFLIAPILQLIFGIHLAWFPVGGWGDGSIKYLILPIVV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LSLPHAGRISRITRGSMIEVMNQNFIRTAKAKGIGPRLTVMRHALKPALMPVVSYLGPAA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SYLLTGSLVVESIFGLPGIGRYFVNAALNRDYGMVLGTVIFYMVLIVFLNLLVDIAYAWL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPKVRNR
CCHHCCC
>Mature Secondary Structure
MIKYALRRLLSTIPVLWIAVTACFFVLRLAPGGPFDGERPLPPVILKNLAVHYNLDKPLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCHHHH
QQYLIYVGDLLRGDLGPSFASEDFTVAQQIMIGLPYTFTIGTAAFLIAIIVGVAVGCLGA
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LYQNKAPDYILGALILVGVVLPNFLIAPILQLIFGIHLAWFPVGGWGDGSIKYLILPIVV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHH
LSLPHAGRISRITRGSMIEVMNQNFIRTAKAKGIGPRLTVMRHALKPALMPVVSYLGPAA
HHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
SYLLTGSLVVESIFGLPGIGRYFVNAALNRDYGMVLGTVIFYMVLIVFLNLLVDIAYAWL
HHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DPKVRNR
CCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]