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The map label for this gene is oppA [H]

Identifier: 209550413

GI number: 209550413

Start: 2884190

End: 2885770

Strand: Direct

Name: oppA [H]

Synonym: Rleg2_2834

Alternate gene names: 209550413

Gene position: 2884190-2885770 (Clockwise)

Preceding gene: 209550411

Following gene: 209550414

Centisome position: 63.56

GC content: 57.37

Gene sequence:

>1581_bases
ATGAACCAGTTCACGAAAAAATTTCTCGCCTCCGCAATGTTTGGCACATTGCTGGCGTTTTCGGCGCATGCGGCCACGCT
GAATATTCACAATGGCGGCGACCCGCAATCGCTCGATCCGCAGAAGCTGTCCGGCGATTGGGAGAATCGTATCGCCGGCG
ACATTTTCGAAGGCTTGGTCACGGAAGACGCCAAGGACAATCCGGTCCCCGGCCAGGCCGAAAGCTGGACGATTTCACCT
GATGGCAAGGTTTACACCTTCAAGCTTCGCGACGGCATCAAATGGTCCGATGGCCAGCCGGTAACGGCAGGAGACTTCGT
CTTCGCCTTCCAGCGCCTCGTCGACCCGAAGAACGCCGCCGACTATGCCTATCTGCAGTTCACCATCAAAAATGCCGAAA
AGATCAACAAGGGTGAGATTACCGATCTCAATCAGCTCGGCGTCAAGGCAATCGACGACAAGACGCTTGAAATCACCCTC
GAAAACCCGACCCCCTATTTCCTCAACGCTCTGATGCACTACACCGCCTATCCGCTGCCCAAGCACGTGGTCGAGGCGAA
GGGCCAGGATTGGGTCAAGATCGGCAATATCGTCACCAACGGACCTTACAAGCCGGTCGAGTGGGTTCCGGGCTCGCATG
TCACCACGGTCAAAAACGACCAGTGGTACGGCACCAAGGACCTGAAGATCGACGGTGCCAAGTTCTTCGTGCTCGAGGAT
CAGGAAGCGGCACTGAAACGTTACCGCGCCGGCGAATTCGATATCCTCACCGATTTCCCCACCGACCAGTATGAGTGGAT
GAAGAAGAACCTGCCGGGCCAGGCACATGTCGCTCCCTTCTCCGGCCTCTATTACTACGTCATCAATTCGACCAAGCCGC
CCTTCGCCGACAAGCGCGTGCGCCAGGCTCTCTCCATGGCGATCAACCGCGAAGTCATCGGCCCGCAGATTCTCGGCACC
GGCGAACTGCCGGCCTATTCCTGGGTCCCGCCAGGCACGGCAAACTACGGCGAACCGGCCTACGTCTCCTGGAAGGATCT
TCCCTACAAGGACAAGGTCGAAGAAGCCAAGAAGCTGCTGAAGGAAGCCGGTTTCGGTCCGGATCATCCGCTGACAGCCG
AGCTCAAATACAACACCAACGACAATCACAAGCGCATCGCCGTGGCGATCGCCTCGATGTGGAAGCCGCTTGGCGTCAAT
GTCGAACTCGTCAATGCCGAGACCAAGGTCCATTATGACCAGTTGCAGCGCGGCGAAGTGCAGATCGGCCGCGCCGGCTG
GCTGGCCGACTATAACGACCCAGACAACTTCCTGAACCTCCTGGTCACAGGCGTCCAGATGAATTACGGCCGCTGGTCCA
ATCCTGACTACGACAAGATGATCAAGGACGGCAACGCCGAGACCGATCTCGCCAAGCGCGCCGCAATCTTCAAGAAAGCC
GAACAGCTGGCACTGGATGATTCCGCCGCCCTGCCGATCTACTATTATGTTTCGAAGAACGTCGTCTCACCGAAGATCGA
AGGCTTCGTCGATAACATCCAGGACATCCACCGCACCCGCTGGCTGTCGATGAAAGAGTAA

Upstream 100 bases:

>100_bases
ATTCCTTTGCGATAAGGTGCGCACTGACCTGCACGTAAGAGGTCAAATCTCGGGAACAGATGTCTAATAAAGGCGCAAAG
CCTAGAAAGGTGGACCCACC

Downstream 100 bases:

>100_bases
GGGAAAACGGTCCTTGCCGCGCTCCGCGGCAAGGACCGGCCCACGACCATGATCAAATACGCCCTCCGTCGTTTGCTATC
GACGATCCCGGTTCTGTGGA

Product: family 5 extracellular solute-binding protein

Products: ADP; phosphate; oligopeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 526; Mature: 526

Protein sequence:

>526_residues
MNQFTKKFLASAMFGTLLAFSAHAATLNIHNGGDPQSLDPQKLSGDWENRIAGDIFEGLVTEDAKDNPVPGQAESWTISP
DGKVYTFKLRDGIKWSDGQPVTAGDFVFAFQRLVDPKNAADYAYLQFTIKNAEKINKGEITDLNQLGVKAIDDKTLEITL
ENPTPYFLNALMHYTAYPLPKHVVEAKGQDWVKIGNIVTNGPYKPVEWVPGSHVTTVKNDQWYGTKDLKIDGAKFFVLED
QEAALKRYRAGEFDILTDFPTDQYEWMKKNLPGQAHVAPFSGLYYYVINSTKPPFADKRVRQALSMAINREVIGPQILGT
GELPAYSWVPPGTANYGEPAYVSWKDLPYKDKVEEAKKLLKEAGFGPDHPLTAELKYNTNDNHKRIAVAIASMWKPLGVN
VELVNAETKVHYDQLQRGEVQIGRAGWLADYNDPDNFLNLLVTGVQMNYGRWSNPDYDKMIKDGNAETDLAKRAAIFKKA
EQLALDDSAALPIYYYVSKNVVSPKIEGFVDNIQDIHRTRWLSMKE

Sequences:

>Translated_526_residues
MNQFTKKFLASAMFGTLLAFSAHAATLNIHNGGDPQSLDPQKLSGDWENRIAGDIFEGLVTEDAKDNPVPGQAESWTISP
DGKVYTFKLRDGIKWSDGQPVTAGDFVFAFQRLVDPKNAADYAYLQFTIKNAEKINKGEITDLNQLGVKAIDDKTLEITL
ENPTPYFLNALMHYTAYPLPKHVVEAKGQDWVKIGNIVTNGPYKPVEWVPGSHVTTVKNDQWYGTKDLKIDGAKFFVLED
QEAALKRYRAGEFDILTDFPTDQYEWMKKNLPGQAHVAPFSGLYYYVINSTKPPFADKRVRQALSMAINREVIGPQILGT
GELPAYSWVPPGTANYGEPAYVSWKDLPYKDKVEEAKKLLKEAGFGPDHPLTAELKYNTNDNHKRIAVAIASMWKPLGVN
VELVNAETKVHYDQLQRGEVQIGRAGWLADYNDPDNFLNLLVTGVQMNYGRWSNPDYDKMIKDGNAETDLAKRAAIFKKA
EQLALDDSAALPIYYYVSKNVVSPKIEGFVDNIQDIHRTRWLSMKE
>Mature_526_residues
MNQFTKKFLASAMFGTLLAFSAHAATLNIHNGGDPQSLDPQKLSGDWENRIAGDIFEGLVTEDAKDNPVPGQAESWTISP
DGKVYTFKLRDGIKWSDGQPVTAGDFVFAFQRLVDPKNAADYAYLQFTIKNAEKINKGEITDLNQLGVKAIDDKTLEITL
ENPTPYFLNALMHYTAYPLPKHVVEAKGQDWVKIGNIVTNGPYKPVEWVPGSHVTTVKNDQWYGTKDLKIDGAKFFVLED
QEAALKRYRAGEFDILTDFPTDQYEWMKKNLPGQAHVAPFSGLYYYVINSTKPPFADKRVRQALSMAINREVIGPQILGT
GELPAYSWVPPGTANYGEPAYVSWKDLPYKDKVEEAKKLLKEAGFGPDHPLTAELKYNTNDNHKRIAVAIASMWKPLGVN
VELVNAETKVHYDQLQRGEVQIGRAGWLADYNDPDNFLNLLVTGVQMNYGRWSNPDYDKMIKDGNAETDLAKRAAIFKKA
EQLALDDSAALPIYYYVSKNVVSPKIEGFVDNIQDIHRTRWLSMKE

Specific function: This protein is a component of the oligopeptide permease, a binding protein-dependent transport system, it binds peptides up to five amino acids long with high affinity [H]

COG id: COG4166

COG function: function code E; ABC-type oligopeptide transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1787495, Length=532, Percent_Identity=41.1654135338346, Blast_Score=395, Evalue=1e-111,
Organism=Escherichia coli, GI87081878, Length=524, Percent_Identity=38.5496183206107, Blast_Score=363, Evalue=1e-101,
Organism=Escherichia coli, GI1789397, Length=500, Percent_Identity=39, Blast_Score=338, Evalue=6e-94,
Organism=Escherichia coli, GI1789966, Length=531, Percent_Identity=26.5536723163842, Blast_Score=155, Evalue=4e-39,
Organism=Escherichia coli, GI1787762, Length=516, Percent_Identity=26.3565891472868, Blast_Score=122, Evalue=4e-29,
Organism=Escherichia coli, GI1787052, Length=512, Percent_Identity=25.1953125, Blast_Score=114, Evalue=2e-26,
Organism=Escherichia coli, GI1789887, Length=474, Percent_Identity=26.3713080168776, Blast_Score=102, Evalue=4e-23,
Organism=Escherichia coli, GI1787551, Length=500, Percent_Identity=24, Blast_Score=96, Evalue=5e-21,

Paralogues:

None

Copy number: 760 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2360 Molecules/Cell In: Stationary-Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 59097; Mature: 59097

Theoretical pI: Translated: 5.87; Mature: 5.87

Prosite motif: PS01040 SBP_BACTERIAL_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQFTKKFLASAMFGTLLAFSAHAATLNIHNGGDPQSLDPQKLSGDWENRIAGDIFEGLV
CCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
TEDAKDNPVPGQAESWTISPDGKVYTFKLRDGIKWSDGQPVTAGDFVFAFQRLVDPKNAA
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DYAYLQFTIKNAEKINKGEITDLNQLGVKAIDDKTLEITLENPTPYFLNALMHYTAYPLP
CEEEEEEEECCCCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHCCCCH
KHVVEAKGQDWVKIGNIVTNGPYKPVEWVPGSHVTTVKNDQWYGTKDLKIDGAKFFVLED
HHHHHCCCCCEEEECCEEECCCCCCCEECCCCEEEEECCCCCCCCCCEEECCEEEEEEEC
QEAALKRYRAGEFDILTDFPTDQYEWMKKNLPGQAHVAPFSGLYYYVINSTKPPFADKRV
HHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCHHHHH
RQALSMAINREVIGPQILGTGELPAYSWVPPGTANYGEPAYVSWKDLPYKDKVEEAKKLL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
KEAGFGPDHPLTAELKYNTNDNHKRIAVAIASMWKPLGVNVELVNAETKVHYDQLQRGEV
HHCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCCEEEECCCHHEEHHHHCCCCE
QIGRAGWLADYNDPDNFLNLLVTGVQMNYGRWSNPDYDKMIKDGNAETDLAKRAAIFKKA
EECCEECCCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
EQLALDDSAALPIYYYVSKNVVSPKIEGFVDNIQDIHRTRWLSMKE
HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNQFTKKFLASAMFGTLLAFSAHAATLNIHNGGDPQSLDPQKLSGDWENRIAGDIFEGLV
CCHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCCHHHCCCCHHHHHHHHHHHHHH
TEDAKDNPVPGQAESWTISPDGKVYTFKLRDGIKWSDGQPVTAGDFVFAFQRLVDPKNAA
CCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCC
DYAYLQFTIKNAEKINKGEITDLNQLGVKAIDDKTLEITLENPTPYFLNALMHYTAYPLP
CEEEEEEEECCCCCCCCCCCCCHHHCCCEEECCCEEEEEECCCCHHHHHHHHHHHCCCCH
KHVVEAKGQDWVKIGNIVTNGPYKPVEWVPGSHVTTVKNDQWYGTKDLKIDGAKFFVLED
HHHHHCCCCCEEEECCEEECCCCCCCEECCCCEEEEECCCCCCCCCCEEECCEEEEEEEC
QEAALKRYRAGEFDILTDFPTDQYEWMKKNLPGQAHVAPFSGLYYYVINSTKPPFADKRV
HHHHHHHHCCCCEEEEECCCCHHHHHHHHCCCCCEEECCCCCEEEEEEECCCCCCHHHHH
RQALSMAINREVIGPQILGTGELPAYSWVPPGTANYGEPAYVSWKDLPYKDKVEEAKKLL
HHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHH
KEAGFGPDHPLTAELKYNTNDNHKRIAVAIASMWKPLGVNVELVNAETKVHYDQLQRGEV
HHCCCCCCCCEEEEEEECCCCCCCEEEEEEHHHCCCCCCCEEEECCCHHEEHHHHCCCCE
QIGRAGWLADYNDPDNFLNLLVTGVQMNYGRWSNPDYDKMIKDGNAETDLAKRAAIFKKA
EECCEECCCCCCCHHHHHHHHHHHEEECCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHH
EQLALDDSAALPIYYYVSKNVVSPKIEGFVDNIQDIHRTRWLSMKE
HHHHCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; oligopeptides [Periplasm]; H2O [C]

Specific reaction: ATP + oligopeptides [Periplasm] + H2O = ADP + phosphate + oligopeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187863; 9097039; 9278503; 2015910; 9298646; 9600841 [H]