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The map label for this gene is livJ [C]

Identifier: 209550409

GI number: 209550409

Start: 2878863

End: 2879966

Strand: Direct

Name: livJ [C]

Synonym: Rleg2_2830

Alternate gene names: 209550409

Gene position: 2878863-2879966 (Clockwise)

Preceding gene: 209550407

Following gene: 209550410

Centisome position: 63.44

GC content: 63.77

Gene sequence:

>1104_bases
ATGACCCTCAAGACATTGACGGCGACACTCGTCGCGTCGCTTGCCTTCGCGCCGCTTGCCCATGCCGATATCACCATCGG
CCTGATTGCGCCGCTGACCGGCCCCGTCGCCGCCTATGGCGACCAGGTGAAGAACGGCGCTCAGACCGCCGTCGACGAGA
TCAACAAGAAGGGCGGCATTCTCGGCGAGAAGGTCGTCCTCGAGCTGGCCGACGATGCCGGCGAACCGAAGCAGGGCGTT
TCCGCCGCCAACAAGGTCGTCGCCGACGGCATTCGCTTCGTCGTCGGCCCGGTGACCTCGGGTGTTGCCATTCCGGTTTC
AGACGTGCTGGCTGAAAACGGTGTGCTGATGGTCACCCCGACTGCAACGGCCCCCGACCTCACCAAGCGCGGCCTCACCA
ACGTGCTGCGCACCTGCGGCCGCGACGACCAGCAGGCCGAAGTCGCTGCCAAATACGTGCTGAAGAATTTCAAGGATAAG
CGCGTCGCCATCGTCAACGACAAGGGCGCCTATGGCAAAGGCCTTGCCGACGCCTTCAAGGCGACGCTGAACGCCGGTGG
CATGACCGAAGTCGTCAATGACGCGGTCACTCCAGGCGACAAGGATTTCAGCGCCCTCACCACCCGCGTCAAATCGGAAA
AGGTCGACCTGGTCTATTTCGGCGGCTATCACCCGGAAGGCGGCCTGCTCGCCCGCCAGCTACATGACCTCGCCGCCAAC
GCGACGATCATCGGCGGCGACGGCCTCTCCAACACCGAATTCTGGGCGATCGGCACGGATGCGGCGGCAGGCACCCTATT
CACCAATGCCTCCGACGCCACCAAGAACCCGGATTCCAAGGCTGCCGCTGATGCGCTCGCTGCCAAGAACATCCCGGCCG
AGGCCTTCACGCTCAACGCCTATGCCGCCGTCGAAGTGCTGAAAGCCGGCATCGAGAAGGCCGGCAGCGCCGAGGATGCG
GAAGCCGTGGCGACCGCGCTGAAAGACGGCAAGGAAATCCCGACTGCTATCGGCAAGGTCACCTATGGCGAAACCGGTGA
CCTGACCTCGCAGAGCTTCTCGCTCTACAAATGGGAAGGCGGCAAGATCGTCGCCGCCGAGTAA

Upstream 100 bases:

>100_bases
TCGATCGTCCTTGGCACGGCCCAAAGGGCCGTGGAATGATCGCAAAAACCGGCGCGGCACCTCATCGCGGGTCGAAGGAC
CCCACCCAAGGAGACAGACA

Downstream 100 bases:

>100_bases
ACTATCGCTGATCGCCATCGACCGGGCGCCCCCGTGGCGCCCGGTTTCGTTTGCGGCACTCGCTTGTGGCGGCAGATCGG
CTATAAGTTTGAGAATCGGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 367; Mature: 366

Protein sequence:

>367_residues
MTLKTLTATLVASLAFAPLAHADITIGLIAPLTGPVAAYGDQVKNGAQTAVDEINKKGGILGEKVVLELADDAGEPKQGV
SAANKVVADGIRFVVGPVTSGVAIPVSDVLAENGVLMVTPTATAPDLTKRGLTNVLRTCGRDDQQAEVAAKYVLKNFKDK
RVAIVNDKGAYGKGLADAFKATLNAGGMTEVVNDAVTPGDKDFSALTTRVKSEKVDLVYFGGYHPEGGLLARQLHDLAAN
ATIIGGDGLSNTEFWAIGTDAAAGTLFTNASDATKNPDSKAAADALAAKNIPAEAFTLNAYAAVEVLKAGIEKAGSAEDA
EAVATALKDGKEIPTAIGKVTYGETGDLTSQSFSLYKWEGGKIVAAE

Sequences:

>Translated_367_residues
MTLKTLTATLVASLAFAPLAHADITIGLIAPLTGPVAAYGDQVKNGAQTAVDEINKKGGILGEKVVLELADDAGEPKQGV
SAANKVVADGIRFVVGPVTSGVAIPVSDVLAENGVLMVTPTATAPDLTKRGLTNVLRTCGRDDQQAEVAAKYVLKNFKDK
RVAIVNDKGAYGKGLADAFKATLNAGGMTEVVNDAVTPGDKDFSALTTRVKSEKVDLVYFGGYHPEGGLLARQLHDLAAN
ATIIGGDGLSNTEFWAIGTDAAAGTLFTNASDATKNPDSKAAADALAAKNIPAEAFTLNAYAAVEVLKAGIEKAGSAEDA
EAVATALKDGKEIPTAIGKVTYGETGDLTSQSFSLYKWEGGKIVAAE
>Mature_366_residues
TLKTLTATLVASLAFAPLAHADITIGLIAPLTGPVAAYGDQVKNGAQTAVDEINKKGGILGEKVVLELADDAGEPKQGVS
AANKVVADGIRFVVGPVTSGVAIPVSDVLAENGVLMVTPTATAPDLTKRGLTNVLRTCGRDDQQAEVAAKYVLKNFKDKR
VAIVNDKGAYGKGLADAFKATLNAGGMTEVVNDAVTPGDKDFSALTTRVKSEKVDLVYFGGYHPEGGLLARQLHDLAANA
TIIGGDGLSNTEFWAIGTDAAAGTLFTNASDATKNPDSKAAADALAAKNIPAEAFTLNAYAAVEVLKAGIEKAGSAEDAE
AVATALKDGKEIPTAIGKVTYGETGDLTSQSFSLYKWEGGKIVAAE

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=358, Percent_Identity=42.1787709497207, Blast_Score=271, Evalue=6e-74,
Organism=Escherichia coli, GI1789867, Length=358, Percent_Identity=39.9441340782123, Blast_Score=263, Evalue=2e-71,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 37619; Mature: 37488

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
1.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLKTLTATLVASLAFAPLAHADITIGLIAPLTGPVAAYGDQVKNGAQTAVDEINKKGGI
CCHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCC
LGEKVVLELADDAGEPKQGVSAANKVVADGIRFVVGPVTSGVAIPVSDVLAENGVLMVTP
CHHHHHEEECCCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCEECHHHHHHCCCEEEEEC
TATAPDLTKRGLTNVLRTCGRDDQQAEVAAKYVLKNFKDKRVAIVNDKGAYGKGLADAFK
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
ATLNAGGMTEVVNDAVTPGDKDFSALTTRVKSEKVDLVYFGGYHPEGGLLARQLHDLAAN
HHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCC
ATIIGGDGLSNTEFWAIGTDAAAGTLFTNASDATKNPDSKAAADALAAKNIPAEAFTLNA
CEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHEEEHH
YAAVEVLKAGIEKAGSAEDAEAVATALKDGKEIPTAIGKVTYGETGDLTSQSFSLYKWEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCEECCCCCCCCCCCCEEEEECC
GKIVAAE
CEEEECC
>Mature Secondary Structure 
TLKTLTATLVASLAFAPLAHADITIGLIAPLTGPVAAYGDQVKNGAQTAVDEINKKGGI
CHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHCCCHHHHHHHHHHCCCC
LGEKVVLELADDAGEPKQGVSAANKVVADGIRFVVGPVTSGVAIPVSDVLAENGVLMVTP
CHHHHHEEECCCCCCCHHHHHHHHHHHHCCHHEEECCCCCCCEECHHHHHHCCCEEEEEC
TATAPDLTKRGLTNVLRTCGRDDQQAEVAAKYVLKNFKDKRVAIVNDKGAYGKGLADAFK
CCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHHHHHH
ATLNAGGMTEVVNDAVTPGDKDFSALTTRVKSEKVDLVYFGGYHPEGGLLARQLHDLAAN
HHHCCCCHHHHHHHCCCCCCCCHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHHCC
ATIIGGDGLSNTEFWAIGTDAAAGTLFTNASDATKNPDSKAAADALAAKNIPAEAFTLNA
CEEEECCCCCCCEEEEEECCCCCCEEEECCCCCCCCCCHHHHHHHHHHCCCCCHHEEEHH
YAAVEVLKAGIEKAGSAEDAEAVATALKDGKEIPTAIGKVTYGETGDLTSQSFSLYKWEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHCCCHHHHHHCCEECCCCCCCCCCCCEEEEECC
GKIVAAE
CEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11756688 [H]