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The map label for this gene is dacD [H]

Identifier: 209549400

GI number: 209549400

Start: 1854246

End: 1855541

Strand: Reverse

Name: dacD [H]

Synonym: Rleg2_1806

Alternate gene names: 209549400

Gene position: 1855541-1854246 (Counterclockwise)

Preceding gene: 209549401

Following gene: 209549399

Centisome position: 40.89

GC content: 62.81

Gene sequence:

>1296_bases
ATGCTGAAGCCTGTGCTTCGCCGTTTAGACCAGTTCAGCTTCCTATCGAAGCGCAGAACCGCTCTAACCTTTTGTTTCCA
CGCAATTCCGGACGCAAAACCGCTGCGCACTTTTGCTGGAATTGCTTTGGCATGCCTCCTGCCGTTGACCACCGGAGCGC
TTGCGGCCGATAGCGGCGCCGCCGGTTTCGCCACCAAGGCGGCGCAGGCCTATATGATCGAGGCCGCCACCGGCACGGTG
CTTCTCGCCAAGAACGAGGACCAGGGCTTTTCGCCGGCTTCGCTTGCGAAGCTGATGACGATGGATCTCGTCTTCGACGC
GGTGACGAAGGGTCAGATCACCTTCGATACCGAATATCCCGTTTCCGAATATGCCTGGAGAACCGGCGGCGCGCCGTCAA
GGACGGCAACGATGTTTGCCAGCCTCAAATCGCGCGTGCGCGTCGAGGACCTGATCAAAGGGGTCGCCATCCAGGGCGCC
AATGACAGTTGCATTATCCTCGCCGAAGGCATGGCCGGCAGCGAGCAGCAATTTGCCGAGTCGATGACGCGGCGCGCCCG
TGAGCTCGGCATGGAGAAAGCCGAGTTCGGCAATTCCACCGGCCTTCCCGATGGAAAGAGCAAGGTGACGGCGCGCGAGA
TGGTGACGCTCGCCACGGCCCTGCAAAAGTCCTATCCGAACCTCTATCCCTATTTTGCGCAGCCGGATTTCGAGTGGAAC
AAGATCTTCCAGCGCAACCGCAATCCGCTGCTCGGGCTCGATCTCGGCGCCGACGGGCTGGCAACCGGCTTTACCGAGGG
CGAGGGGTATTCGATCGTCGCGTCGGTTGAGCGGGACGGCAGGCGGCTGTTTCTGGCGCTTGCCGGCGTCGCTTCCGACA
AGGAGCGGACGGAGGAGGCCAAGCGGGTGCTCGAATGGGGGCTGACGGCCTTTGAGAGCCGGCAGATCTTCGCCGAGAAC
GAAGTGATCGGCGCCGCCAGCGTCTATGGCGGCACGGCGCGCACAGTCGATCTCGTGGCCAAGGCGCCGGTCAGCGTCTA
TATTCCGATCAGCAATCCCGACCGGCTGTCGGCGCGCATCATCTATCGCTGGCCGCTGGCAGCACCGGTGCAACCGGATA
CCCAGGCAGGTACGCTCAGGATTTTTGCAGGCAGCCGGCTGCTCAGGGAAGTGCCGCTCTATACCGTGCAGGCGGTCGGC
GAGGGCTCGCTCAGCAGCCGGGCTGTTGACGCCATGCTGGAACTCGGCGAATCGCTGTTCTTCTCCTGGCTCTGGGACAA
GTCCGCGCCGGGCTGA

Upstream 100 bases:

>100_bases
GTCAGGCCAAATTCGCCGCGCGCTGAGCGCGGCGATTGCATCGGATTTCCTCTCGCTCTAACCTCCTGACGACACGCCGC
TCGATCAATGGAAACTCCTG

Downstream 100 bases:

>100_bases
GCGCCGGTTTTCCGATGCAATTTGCCGGGAAAGGTGAATATGTCGCCGCGGCGAAAGGTTTTCCTTCCTGCTGTCTTCGG
ATAGATAGAAGAAGCAAGGC

Product: serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein 6b; PBP-6b [H]

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MLKPVLRRLDQFSFLSKRRTALTFCFHAIPDAKPLRTFAGIALACLLPLTTGALAADSGAAGFATKAAQAYMIEAATGTV
LLAKNEDQGFSPASLAKLMTMDLVFDAVTKGQITFDTEYPVSEYAWRTGGAPSRTATMFASLKSRVRVEDLIKGVAIQGA
NDSCIILAEGMAGSEQQFAESMTRRARELGMEKAEFGNSTGLPDGKSKVTAREMVTLATALQKSYPNLYPYFAQPDFEWN
KIFQRNRNPLLGLDLGADGLATGFTEGEGYSIVASVERDGRRLFLALAGVASDKERTEEAKRVLEWGLTAFESRQIFAEN
EVIGAASVYGGTARTVDLVAKAPVSVYIPISNPDRLSARIIYRWPLAAPVQPDTQAGTLRIFAGSRLLREVPLYTVQAVG
EGSLSSRAVDAMLELGESLFFSWLWDKSAPG

Sequences:

>Translated_431_residues
MLKPVLRRLDQFSFLSKRRTALTFCFHAIPDAKPLRTFAGIALACLLPLTTGALAADSGAAGFATKAAQAYMIEAATGTV
LLAKNEDQGFSPASLAKLMTMDLVFDAVTKGQITFDTEYPVSEYAWRTGGAPSRTATMFASLKSRVRVEDLIKGVAIQGA
NDSCIILAEGMAGSEQQFAESMTRRARELGMEKAEFGNSTGLPDGKSKVTAREMVTLATALQKSYPNLYPYFAQPDFEWN
KIFQRNRNPLLGLDLGADGLATGFTEGEGYSIVASVERDGRRLFLALAGVASDKERTEEAKRVLEWGLTAFESRQIFAEN
EVIGAASVYGGTARTVDLVAKAPVSVYIPISNPDRLSARIIYRWPLAAPVQPDTQAGTLRIFAGSRLLREVPLYTVQAVG
EGSLSSRAVDAMLELGESLFFSWLWDKSAPG
>Mature_431_residues
MLKPVLRRLDQFSFLSKRRTALTFCFHAIPDAKPLRTFAGIALACLLPLTTGALAADSGAAGFATKAAQAYMIEAATGTV
LLAKNEDQGFSPASLAKLMTMDLVFDAVTKGQITFDTEYPVSEYAWRTGGAPSRTATMFASLKSRVRVEDLIKGVAIQGA
NDSCIILAEGMAGSEQQFAESMTRRARELGMEKAEFGNSTGLPDGKSKVTAREMVTLATALQKSYPNLYPYFAQPDFEWN
KIFQRNRNPLLGLDLGADGLATGFTEGEGYSIVASVERDGRRLFLALAGVASDKERTEEAKRVLEWGLTAFESRQIFAEN
EVIGAASVYGGTARTVDLVAKAPVSVYIPISNPDRLSARIIYRWPLAAPVQPDTQAGTLRIFAGSRLLREVPLYTVQAVG
EGSLSSRAVDAMLELGESLFFSWLWDKSAPG

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Potential). Note=N-terminal lies in the periplasmic space (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI87082022, Length=342, Percent_Identity=31.5789473684211, Blast_Score=180, Evalue=2e-46,
Organism=Escherichia coli, GI1787062, Length=384, Percent_Identity=32.5520833333333, Blast_Score=173, Evalue=2e-44,
Organism=Escherichia coli, GI1786851, Length=361, Percent_Identity=29.3628808864266, Blast_Score=155, Evalue=4e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 46657; Mature: 46657

Theoretical pI: Translated: 7.19; Mature: 7.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKPVLRRLDQFSFLSKRRTALTFCFHAIPDAKPLRTFAGIALACLLPLTTGALAADSGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGFATKAAQAYMIEAATGTVLLAKNEDQGFSPASLAKLMTMDLVFDAVTKGQITFDTEYP
CCHHHHHHHEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
VSEYAWRTGGAPSRTATMFASLKSRVRVEDLIKGVAIQGANDSCIILAEGMAGSEQQFAE
HHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCEEEECCCCCEEEEECCCCCCHHHHHH
SMTRRARELGMEKAEFGNSTGLPDGKSKVTAREMVTLATALQKSYPNLYPYFAQPDFEWN
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
KIFQRNRNPLLGLDLGADGLATGFTEGEGYSIVASVERDGRRLFLALAGVASDKERTEEA
HHHHCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHH
KRVLEWGLTAFESRQIFAENEVIGAASVYGGTARTVDLVAKAPVSVYIPISNPDRLSARI
HHHHHHCCHHHHCCEEEECCCEEEEHHHCCCCCCEEEHEECCCEEEEEECCCCCCCEEEE
IYRWPLAAPVQPDTQAGTLRIFAGSRLLREVPLYTVQAVGEGSLSSRAVDAMLELGESLF
EEEECCCCCCCCCCCCCEEEEEECCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
FSWLWDKSAPG
HHHHCCCCCCC
>Mature Secondary Structure
MLKPVLRRLDQFSFLSKRRTALTFCFHAIPDAKPLRTFAGIALACLLPLTTGALAADSGA
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCC
AGFATKAAQAYMIEAATGTVLLAKNEDQGFSPASLAKLMTMDLVFDAVTKGQITFDTEYP
CCHHHHHHHEEEEEECCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCC
VSEYAWRTGGAPSRTATMFASLKSRVRVEDLIKGVAIQGANDSCIILAEGMAGSEQQFAE
HHHHHCCCCCCCCHHHHHHHHHHHHCCHHHHHCCEEEECCCCCEEEEECCCCCCHHHHHH
SMTRRARELGMEKAEFGNSTGLPDGKSKVTAREMVTLATALQKSYPNLYPYFAQPDFEWN
HHHHHHHHHCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHH
KIFQRNRNPLLGLDLGADGLATGFTEGEGYSIVASVERDGRRLFLALAGVASDKERTEEA
HHHHCCCCCEEEEEECCCCHHCCCCCCCCEEEEEEECCCCCEEEEEEECCCCCHHHHHHH
KRVLEWGLTAFESRQIFAENEVIGAASVYGGTARTVDLVAKAPVSVYIPISNPDRLSARI
HHHHHHCCHHHHCCEEEECCCEEEEHHHCCCCCCEEEHEECCCEEEEEECCCCCCCEEEE
IYRWPLAAPVQPDTQAGTLRIFAGSRLLREVPLYTVQAVGEGSLSSRAVDAMLELGESLF
EEEECCCCCCCCCCCCCEEEEEECCHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHH
FSWLWDKSAPG
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8955390; 9097040; 9278503 [H]