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The map label for this gene is tmk [H]

Identifier: 209549399

GI number: 209549399

Start: 1853433

End: 1854113

Strand: Reverse

Name: tmk [H]

Synonym: Rleg2_1805

Alternate gene names: 209549399

Gene position: 1854113-1853433 (Counterclockwise)

Preceding gene: 209549400

Following gene: 209549398

Centisome position: 40.86

GC content: 65.2

Gene sequence:

>681_bases
TTGTCATCCGGTACGGGATTGTTCGTTACGTTTGAAGGTGGGGAAGGCGCTGGGAAATCCACGCAGATCCGCCGCCTCGC
CGAGGCGCTGAAGGGCGACGGTCACGATGTGCTGGTGACGCGGGAACCGGGCGGATCGCCGGGCGCCGAGGCCGTGCGCC
ACGTGCTCCTGTCGGGGGCTGCTGAGGCCTTCGGCACGCGGATGGAGGCAATCCTGTTTGCGGCGGCGCGCAACGACCAT
GTCGAAGAGGTGATCCGCCCCGCACTGGCTGCCGGCAAGATCGTGCTCTGCGACCGTTTCATGGATTCCTCCCGCGTCTA
CCAGGGCGTCACCGGCAATCTCCAGCCCGATTTCATCGAGACGCTGCAGCGCATCGCCATCAACGGCGTCATGCCGGATT
GCACTGTGATCCTCGACATCCCCGCCAAGATCGGGCTGGAGCGCGCGCAGAAACGCGCCGCCACCGACGCTCCCGATCGC
TTCGAGAAAGAGCGGCTTGAAACCCACGAGAAACGGCGCGAAGCCTTTCTCGATATCGCCGCCCGCGAGCCGGAGCGCTG
TCATGTGATCAACGCCATGCAGAGCGAGGAAGCAATCGCCGCCGAGATCGTGGCGATCGTCCGGCAGCTCATGTCGCCTG
CAGGCCGTGCGCGCATGCCGGAAGCCGCACATCATGAGTGA

Upstream 100 bases:

>100_bases
GGTGAATATGTCGCCGCGGCGAAAGGTTTTCCTTCCTGCTGTCTTCGGATAGATAGAAGAAGCAAGGCGCGTACGGCGCC
TGGAATGCGGGAAGTTGTCA

Downstream 100 bases:

>100_bases
ATCCCGTTATGAGTGAAGAAAGGCCCGGACTGCTCGACGGCGCCATCTGGCCGGGCGAAAATACCAGGCTGTTCGGCCAT
GAGGAGGCGCAGGCCTTCCT

Product: thymidylate kinase

Products: NA

Alternate protein names: dTMP kinase [H]

Number of amino acids: Translated: 226; Mature: 225

Protein sequence:

>226_residues
MSSGTGLFVTFEGGEGAGKSTQIRRLAEALKGDGHDVLVTREPGGSPGAEAVRHVLLSGAAEAFGTRMEAILFAAARNDH
VEEVIRPALAAGKIVLCDRFMDSSRVYQGVTGNLQPDFIETLQRIAINGVMPDCTVILDIPAKIGLERAQKRAATDAPDR
FEKERLETHEKRREAFLDIAAREPERCHVINAMQSEEAIAAEIVAIVRQLMSPAGRARMPEAAHHE

Sequences:

>Translated_226_residues
MSSGTGLFVTFEGGEGAGKSTQIRRLAEALKGDGHDVLVTREPGGSPGAEAVRHVLLSGAAEAFGTRMEAILFAAARNDH
VEEVIRPALAAGKIVLCDRFMDSSRVYQGVTGNLQPDFIETLQRIAINGVMPDCTVILDIPAKIGLERAQKRAATDAPDR
FEKERLETHEKRREAFLDIAAREPERCHVINAMQSEEAIAAEIVAIVRQLMSPAGRARMPEAAHHE
>Mature_225_residues
SSGTGLFVTFEGGEGAGKSTQIRRLAEALKGDGHDVLVTREPGGSPGAEAVRHVLLSGAAEAFGTRMEAILFAAARNDHV
EEVIRPALAAGKIVLCDRFMDSSRVYQGVTGNLQPDFIETLQRIAINGVMPDCTVILDIPAKIGLERAQKRAATDAPDRF
EKERLETHEKRREAFLDIAAREPERCHVINAMQSEEAIAAEIVAIVRQLMSPAGRARMPEAAHHE

Specific function: Phosphorylation of dTMP to form dTDP in both de novo and salvage pathways of dTTP synthesis [H]

COG id: COG0125

COG function: function code F; Thymidylate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thymidylate kinase family [H]

Homologues:

Organism=Escherichia coli, GI1787340, Length=200, Percent_Identity=36, Blast_Score=110, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018095
- InterPro:   IPR018094 [H]

Pfam domain/function: PF02223 Thymidylate_kin [H]

EC number: =2.7.4.9 [H]

Molecular weight: Translated: 24503; Mature: 24372

Theoretical pI: Translated: 5.91; Mature: 5.91

Prosite motif: PS01331 THYMIDYLATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSGTGLFVTFEGGEGAGKSTQIRRLAEALKGDGHDVLVTREPGGSPGAEAVRHVLLSGA
CCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
AEAFGTRMEAILFAAARNDHVEEVIRPALAAGKIVLCDRFMDSSRVYQGVTGNLQPDFIE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHCHHHHHCCCCCCCCHHHHH
TLQRIAINGVMPDCTVILDIPAKIGLERAQKRAATDAPDRFEKERLETHEKRREAFLDIA
HHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AREPERCHVINAMQSEEAIAAEIVAIVRQLMSPAGRARMPEAAHHE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SSGTGLFVTFEGGEGAGKSTQIRRLAEALKGDGHDVLVTREPGGSPGAEAVRHVLLSGA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHHHHH
AEAFGTRMEAILFAAARNDHVEEVIRPALAAGKIVLCDRFMDSSRVYQGVTGNLQPDFIE
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCEEEHHHHHCHHHHHCCCCCCCCHHHHH
TLQRIAINGVMPDCTVILDIPAKIGLERAQKRAATDAPDRFEKERLETHEKRREAFLDIA
HHHHHHHCCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
AREPERCHVINAMQSEEAIAAEIVAIVRQLMSPAGRARMPEAAHHE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11481430 [H]