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The map label for this gene is ispDF [H]

Identifier: 209549215

GI number: 209549215

Start: 1640906

End: 1642126

Strand: Reverse

Name: ispDF [H]

Synonym: Rleg2_1616

Alternate gene names: 209549215

Gene position: 1642126-1640906 (Counterclockwise)

Preceding gene: 209549220

Following gene: 209549214

Centisome position: 36.19

GC content: 63.39

Gene sequence:

>1221_bases
ATGCTGCAAATGCCGTCCAAGCAACCGATATCGGCTGGAATTGTCATCGTTGCCGCCGGCCGCGGCGAACGTGCGGGATC
CTCCAAGGAAGGCCCCAAGCAGTATCGCATGATCGGCGGCAAGCCGGTTATCGTGCATACGCTTGAAAACTTCATGACAT
GGGAGCCGGCAACTGAGATCGTCGTCGTCATTCACCCCGATGACGAGGCGCTGTTTGCCAGAGCATTGCGCCATATCATT
TCGGCAACGCCGATCGAAACGGTGCATGGCGGGCCGACCCGACAGCAATCGGTGCTGGCCGGCCTTAGGCACCTCAAGGA
CAAGCGTATCAGCCATGTGCTGATCCACGACGCCGTGCGGCCGTTCTTCGACCATGTGCTTCTCGACCGCATTGCCGAAA
GCCTCGACAATGGCGCGCAGGCGGTGCTGCCGGCGATCCCGGTCACCGACACGCTGAAACGCGCCGACAGCGCCGGCACC
GTGCTGACGACGGTTTCACGCGAGCATCTTTACGCGGCGCAGACGCCGCAATCCTTCGCTTTTGAAACGATCCTCGATGC
GCATGAGAAGGCGGCGGCAAGCGGGCGAAGCGATTTCACCGACGACGCTTCGATCGCCGAATGGGCGGGCATTCCGGTGA
CGATCGTCGCGGGCACGCCCGATAACGTCAAGCTGACGGTCAAGAAGGATATCGCCATGGCGGACGACAAGCTATCGGCC
TCGCTGCTTCCCGACGTGCGCACCGGCAACGGCTACGACGTGCACCAGCTGGAAGCGGGCGACGGTGTCACACTCTGCGG
CGTGTTCATTCCGCACGACCAGAAGCTCAAAGGCCATTCGGATGCCGACGTCGCGCTGCACGCGCTGACGGACGCGCTGC
TTGCCACCTGCGGCGCCGGCGATATCGGCGATCATTTCCCGCCCTCCGACCCGCAATGGAAGGGGGCGGCCTCGCGCATC
TTCATCGAGCACGCCGCCCGGATCGTGCGCGAGCACGGCGGCACGATCATGAATGCCGATGTCTCACTGATCGCCGAGGC
GCCGAAGGTCGGGCCTCATCGCGAGAGCATGCGGATGAGACTGTCGGAGTATCTCGGCATCGATATCGAGCGCTGCTCGG
TCAAGGCGACGACCAACGAGACCATCGGCTTCGTCGGCCGGCGCGAAGGCATCGCGGCAATCGCCACGGCGACCGTCGTC
TATCGCGGGGTGAAACGATGA

Upstream 100 bases:

>100_bases
GGGGCTCTGATCGAAATCGATATTTTTCGGGCAGATGCTGGAAAAGGCCAAAGCAAGAGGTTAGAGCACTCGGGTCAATT
CCGCATATTTTAGGGGATTT

Downstream 100 bases:

>100_bases
GCCTTTTTCCCGAGGATATCCTTTCGGCGGCGCAGACGATCATCCGCGACTTCACGGGCGCCGGGCTGATGATTTCGACC
GCCGAATCCTGCACCGGCGG

Product: bifunctional 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase/2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase protein

Products: NA

Alternate protein names: 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase; 4-diphosphocytidyl-2C-methyl-D-erythritol synthase; MEP cytidylyltransferase; MCT; 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase; MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MLQMPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWEPATEIVVVIHPDDEALFARALRHII
SATPIETVHGGPTRQQSVLAGLRHLKDKRISHVLIHDAVRPFFDHVLLDRIAESLDNGAQAVLPAIPVTDTLKRADSAGT
VLTTVSREHLYAAQTPQSFAFETILDAHEKAAASGRSDFTDDASIAEWAGIPVTIVAGTPDNVKLTVKKDIAMADDKLSA
SLLPDVRTGNGYDVHQLEAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRI
FIEHAARIVREHGGTIMNADVSLIAEAPKVGPHRESMRMRLSEYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVV
YRGVKR

Sequences:

>Translated_406_residues
MLQMPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWEPATEIVVVIHPDDEALFARALRHII
SATPIETVHGGPTRQQSVLAGLRHLKDKRISHVLIHDAVRPFFDHVLLDRIAESLDNGAQAVLPAIPVTDTLKRADSAGT
VLTTVSREHLYAAQTPQSFAFETILDAHEKAAASGRSDFTDDASIAEWAGIPVTIVAGTPDNVKLTVKKDIAMADDKLSA
SLLPDVRTGNGYDVHQLEAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRI
FIEHAARIVREHGGTIMNADVSLIAEAPKVGPHRESMRMRLSEYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVV
YRGVKR
>Mature_406_residues
MLQMPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWEPATEIVVVIHPDDEALFARALRHII
SATPIETVHGGPTRQQSVLAGLRHLKDKRISHVLIHDAVRPFFDHVLLDRIAESLDNGAQAVLPAIPVTDTLKRADSAGT
VLTTVSREHLYAAQTPQSFAFETILDAHEKAAASGRSDFTDDASIAEWAGIPVTIVAGTPDNVKLTVKKDIAMADDKLSA
SLLPDVRTGNGYDVHQLEAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAGDIGDHFPPSDPQWKGAASRI
FIEHAARIVREHGGTIMNADVSLIAEAPKVGPHRESMRMRLSEYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVV
YRGVKR

Specific function: Bifunctional enzyme that catalyzes the formation of 4- diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl- D-erythritol 4-phosphate (MEP) (ispD), and converts 4- diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C- methyl-D-erythri

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the ispF family [H]

Homologues:

Organism=Homo sapiens, GI157412259, Length=242, Percent_Identity=27.6859504132231, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1789103, Length=155, Percent_Identity=47.741935483871, Blast_Score=139, Evalue=5e-34,
Organism=Escherichia coli, GI1789104, Length=229, Percent_Identity=33.6244541484716, Blast_Score=103, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001228
- InterPro:   IPR018294
- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF01128 IspD; PF02542 YgbB [H]

EC number: =2.7.7.60; =4.6.1.12 [H]

Molecular weight: Translated: 43577; Mature: 43577

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS01295 ISPD ; PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQMPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWEPATEI
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHCCCCCCCE
VVVIHPDDEALFARALRHIISATPIETVHGGPTRQQSVLAGLRHLKDKRISHVLIHDAVR
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFFDHVLLDRIAESLDNGAQAVLPAIPVTDTLKRADSAGTVLTTVSREHLYAAQTPQSFA
HHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCEEEEECHHHEEECCCCHHHH
FETILDAHEKAAASGRSDFTDDASIAEWAGIPVTIVAGTPDNVKLTVKKDIAMADDKLSA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCH
SLLPDVRTGNGYDVHQLEAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAG
HHCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DIGDHFPPSDPQWKGAASRIFIEHAARIVREHGGTIMNADVSLIAEAPKVGPHRESMRMR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCHHHHHHH
LSEYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVVYRGVKR
HHHHHCCEEEHEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MLQMPSKQPISAGIVIVAAGRGERAGSSKEGPKQYRMIGGKPVIVHTLENFMTWEPATEI
CCCCCCCCCCCCCEEEEECCCCCCCCCCCCCCHHHHHCCCCEEEEEEHHHHHCCCCCCCE
VVVIHPDDEALFARALRHIISATPIETVHGGPTRQQSVLAGLRHLKDKRISHVLIHDAVR
EEEEECCCHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
PFFDHVLLDRIAESLDNGAQAVLPAIPVTDTLKRADSAGTVLTTVSREHLYAAQTPQSFA
HHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHCCCCCCEEEEECHHHEEECCCCHHHH
FETILDAHEKAAASGRSDFTDDASIAEWAGIPVTIVAGTPDNVKLTVKKDIAMADDKLSA
HHHHHHHHHHHHHCCCCCCCCCCCHHHHCCCCEEEEECCCCCEEEEEECCCCCCCCCCCH
SLLPDVRTGNGYDVHQLEAGDGVTLCGVFIPHDQKLKGHSDADVALHALTDALLATCGAG
HHCCCCCCCCCCCEEEEECCCCEEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHCCCC
DIGDHFPPSDPQWKGAASRIFIEHAARIVREHGGTIMNADVSLIAEAPKVGPHRESMRMR
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCEEEECCCEEEECCCCCCCCHHHHHHH
LSEYLGIDIERCSVKATTNETIGFVGRREGIAAIATATVVYRGVKR
HHHHHCCEEEHEEEEECCCCCEECCCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA