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The map label for this gene is phbC [H]

Identifier: 209549141

GI number: 209549141

Start: 1566084

End: 1567976

Strand: Reverse

Name: phbC [H]

Synonym: Rleg2_1542

Alternate gene names: 209549141

Gene position: 1567976-1566084 (Counterclockwise)

Preceding gene: 209549147

Following gene: 209549140

Centisome position: 34.55

GC content: 60.38

Gene sequence:

>1893_bases
ATGTACAACGAATGGATAAAAAGAGTGCTGCCGCCGGAGGAAATGGTGACCGACAGCAAGCAGGAGAATGGCGGCCAGAA
GAATGGCGGCAAGGCCGGTTTCGACGCGACCGATCTCGATCCCTATCTGTTGAAGGATCCCGAGGCGATGGCGATGAATT
TCGCCCGGGCGCTCGAAAATCTCGGCCAGGCGGCCTCGGCCTGGCTTGCGCCGCGCGAACGCGGCGAGATCAGCGAAACC
GTCATCGATCCGATGACCGACATGGTCAAGACGCTTTCCAAGGTCACCGAATACTGGATTGCCGATCCCCGCCGCACCTT
CGAGGCGCAGACGCAGCTGATGTCCTCGTTCTTCGGCATCTGGATGCGCTCAATGCAGCGCATGCAGGGCGAGCCCTCGC
CTCCGGAACCGGACACCCGCAAGGACAAGCGCTTCTCCGACGAGGACTGGCAGAAGAACCCGTTCTTCGATTTTCTCCGC
CAGGTCTATTTCGTCACCACCGACTGGGCGGAGAAGCTGGTGTCGGAGACCGACGGCCTCGACGAGCACACCAAGCACAA
GGCCGGCTTCTATGTGAAGCAGATCACGGCAGCACTTTCGCCGAGCAATTTTGTCGCCACCAATCCGCAGCTCTACCGCG
AGACGATCGCGACCAGCGGCGAAAACCTCGTCCGCGGGATGAAGATGCTGGCCGAGGATATCGCTGCCGGAAAGGGCGAT
CTTCGCCTTCGCCAGACCGACATGACGAAATTCGCCGTCGGCCGCGACATGGCGCTGACGCCGGGCAAGGTGATCGCCCA
GAACGACATCTGCCAGATCATCCAATATGAGGCTTCAACCGAAACGGTGCTGAAGCGGCCGCTATTGATCTGCCCGCCCT
GGATCAACAAATTCTATATTCTTGACCTCAACCCGCAGAAATCCTTCATCAAATGGTGCGTCGATCAGGGTCAGACCGTT
TTCGTCATCTCCTGGGTCAACCCGGATGCGCGCCATGCCGACAAGGACTGGGCGGCCTATGCCCGCGAGGGCATTGATTT
CGCGCTCGAGACGATCGAGAAGGCGACTGGCGAAAAGGACGTCAATGCCGTCGGCTATTGCGTCGGCGGTACACTTCTGG
CGGCAACGCTGGCGCTGCACGCCAAGGAGAAGAACAAGCGCATCAAAACCGCGACGCTCTTCACCACCCAGGTCGATTTC
ACCCATGCCGGCGATCTCAAGGTCTTCGTCGACGAGGAGCAGCTCGCAGCGCTCGAAGAGCATATGCAGGCGACCGGTTA
TCTCGACGGCTCGAAGATGTCGATGGCCTTCAACATGCTGCGCGCTTCCGAGCTGATCTGGCCTTATTTCGTCAACAGCT
ACCTCAAGGGCCAGGATCCCCTGCCCTTCGACCTGTTGTTCTGGAACGCCGATTCGACCCGCATGGCGGCGGCAAACCAT
GCCTTCTACCTGCGCAACTGCTATCTCAGGAACGCGCTGACCCGCAACGAGATGATCCTCGACGGCAAGCCGGTGTCGCT
GAAGGATGTGAAGATCCCGATCTATAATCTCGCCACCCGCGAGGACCACATCGCCCCTGCCAAATCCGTCTTCGTCGGCA
GCCAGTTCTTCGGCGGCAAGGTGGAATTCGTTGTCACCGGCTCGGGACATATCGCCGGCGTGGTCAACCCGCCCGACAAG
AAGAAGTATCAATTCTGGACGGGCGGCCCGGCCAAGGGCGAGTATGAAACCTGGATCGACCAGGCGACCGAGACGCCGGG
ATCATGGTGGCCGCATTGGCGGGCCTGGATCGAGACCCATGACGGCAGGCGCGTTGCGGCGCGCAAACCGGGCGGCGACG
CGCTGAATGCGATCGAGGAAGCGCCCGGAAGTTACGTGATGGAACGAGTCTGA

Upstream 100 bases:

>100_bases
CAAAATCCAGTTCTGACTGCGTCGACTGTTAAATTTGTCGCAGCCACAAAGCAATAGCATGAGCCTGCGCGGGTGGAACT
TGTTTTCTCGTTCAATCACG

Downstream 100 bases:

>100_bases
AAGATCCCACGCATCTCTTTTTGAGACGTCGTGAGAAAGCGCAACGCCGCGTAAATAAATTAGAAACCATAATTCGTCAT
CCTTGCGAAACAGTCGGATG

Product: poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 630; Mature: 630

Protein sequence:

>630_residues
MYNEWIKRVLPPEEMVTDSKQENGGQKNGGKAGFDATDLDPYLLKDPEAMAMNFARALENLGQAASAWLAPRERGEISET
VIDPMTDMVKTLSKVTEYWIADPRRTFEAQTQLMSSFFGIWMRSMQRMQGEPSPPEPDTRKDKRFSDEDWQKNPFFDFLR
QVYFVTTDWAEKLVSETDGLDEHTKHKAGFYVKQITAALSPSNFVATNPQLYRETIATSGENLVRGMKMLAEDIAAGKGD
LRLRQTDMTKFAVGRDMALTPGKVIAQNDICQIIQYEASTETVLKRPLLICPPWINKFYILDLNPQKSFIKWCVDQGQTV
FVISWVNPDARHADKDWAAYAREGIDFALETIEKATGEKDVNAVGYCVGGTLLAATLALHAKEKNKRIKTATLFTTQVDF
THAGDLKVFVDEEQLAALEEHMQATGYLDGSKMSMAFNMLRASELIWPYFVNSYLKGQDPLPFDLLFWNADSTRMAAANH
AFYLRNCYLRNALTRNEMILDGKPVSLKDVKIPIYNLATREDHIAPAKSVFVGSQFFGGKVEFVVTGSGHIAGVVNPPDK
KKYQFWTGGPAKGEYETWIDQATETPGSWWPHWRAWIETHDGRRVAARKPGGDALNAIEEAPGSYVMERV

Sequences:

>Translated_630_residues
MYNEWIKRVLPPEEMVTDSKQENGGQKNGGKAGFDATDLDPYLLKDPEAMAMNFARALENLGQAASAWLAPRERGEISET
VIDPMTDMVKTLSKVTEYWIADPRRTFEAQTQLMSSFFGIWMRSMQRMQGEPSPPEPDTRKDKRFSDEDWQKNPFFDFLR
QVYFVTTDWAEKLVSETDGLDEHTKHKAGFYVKQITAALSPSNFVATNPQLYRETIATSGENLVRGMKMLAEDIAAGKGD
LRLRQTDMTKFAVGRDMALTPGKVIAQNDICQIIQYEASTETVLKRPLLICPPWINKFYILDLNPQKSFIKWCVDQGQTV
FVISWVNPDARHADKDWAAYAREGIDFALETIEKATGEKDVNAVGYCVGGTLLAATLALHAKEKNKRIKTATLFTTQVDF
THAGDLKVFVDEEQLAALEEHMQATGYLDGSKMSMAFNMLRASELIWPYFVNSYLKGQDPLPFDLLFWNADSTRMAAANH
AFYLRNCYLRNALTRNEMILDGKPVSLKDVKIPIYNLATREDHIAPAKSVFVGSQFFGGKVEFVVTGSGHIAGVVNPPDK
KKYQFWTGGPAKGEYETWIDQATETPGSWWPHWRAWIETHDGRRVAARKPGGDALNAIEEAPGSYVMERV
>Mature_630_residues
MYNEWIKRVLPPEEMVTDSKQENGGQKNGGKAGFDATDLDPYLLKDPEAMAMNFARALENLGQAASAWLAPRERGEISET
VIDPMTDMVKTLSKVTEYWIADPRRTFEAQTQLMSSFFGIWMRSMQRMQGEPSPPEPDTRKDKRFSDEDWQKNPFFDFLR
QVYFVTTDWAEKLVSETDGLDEHTKHKAGFYVKQITAALSPSNFVATNPQLYRETIATSGENLVRGMKMLAEDIAAGKGD
LRLRQTDMTKFAVGRDMALTPGKVIAQNDICQIIQYEASTETVLKRPLLICPPWINKFYILDLNPQKSFIKWCVDQGQTV
FVISWVNPDARHADKDWAAYAREGIDFALETIEKATGEKDVNAVGYCVGGTLLAATLALHAKEKNKRIKTATLFTTQVDF
THAGDLKVFVDEEQLAALEEHMQATGYLDGSKMSMAFNMLRASELIWPYFVNSYLKGQDPLPFDLLFWNADSTRMAAANH
AFYLRNCYLRNALTRNEMILDGKPVSLKDVKIPIYNLATREDHIAPAKSVFVGSQFFGGKVEFVVTGSGHIAGVVNPPDK
KKYQFWTGGPAKGEYETWIDQATETPGSWWPHWRAWIETHDGRRVAARKPGGDALNAIEEAPGSYVMERV

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 71076; Mature: 71076

Theoretical pI: Translated: 5.73; Mature: 5.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNEWIKRVLPPEEMVTDSKQENGGQKNGGKAGFDATDLDPYLLKDPEAMAMNFARALEN
CCHHHHHHHCCHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
LGQAASAWLAPRERGEISETVIDPMTDMVKTLSKVTEYWIADPRRTFEAQTQLMSSFFGI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
WMRSMQRMQGEPSPPEPDTRKDKRFSDEDWQKNPFFDFLRQVYFVTTDWAEKLVSETDGL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHCCCC
DEHTKHKAGFYVKQITAALSPSNFVATNPQLYRETIATSGENLVRGMKMLAEDIAAGKGD
CHHHHHHHCHHHHHHHHHCCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
LRLRQTDMTKFAVGRDMALTPGKVIAQNDICQIIQYEASTETVLKRPLLICPPWINKFYI
EEEECCCCHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCHHHHHHCCCEEECCCCCEEEE
LDLNPQKSFIKWCVDQGQTVFVISWVNPDARHADKDWAAYAREGIDFALETIEKATGEKD
EEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VNAVGYCVGGTLLAATLALHAKEKNKRIKTATLFTTQVDFTHAGDLKVFVDEEQLAALEE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECEEECCCEEEEECHHHHHHHHH
HMQATGYLDGSKMSMAFNMLRASELIWPYFVNSYLKGQDPLPFDLLFWNADSTRMAAANH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCC
AFYLRNCYLRNALTRNEMILDGKPVSLKDVKIPIYNLATREDHIAPAKSVFVGSQFFGGK
HHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEEECCCCCCCCCCHHHHEECCCCCCCE
VEFVVTGSGHIAGVVNPPDKKKYQFWTGGPAKGEYETWIDQATETPGSWWPHWRAWIETH
EEEEEECCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHEEEC
DGRRVAARKPGGDALNAIEEAPGSYVMERV
CCCEEECCCCCCHHHHHHHHCCCHHHHHCC
>Mature Secondary Structure
MYNEWIKRVLPPEEMVTDSKQENGGQKNGGKAGFDATDLDPYLLKDPEAMAMNFARALEN
CCHHHHHHHCCHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHH
LGQAASAWLAPRERGEISETVIDPMTDMVKTLSKVTEYWIADPRRTFEAQTQLMSSFFGI
HHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
WMRSMQRMQGEPSPPEPDTRKDKRFSDEDWQKNPFFDFLRQVYFVTTDWAEKLVSETDGL
HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHEEECHHHHHHHHHCCCC
DEHTKHKAGFYVKQITAALSPSNFVATNPQLYRETIATSGENLVRGMKMLAEDIAAGKGD
CHHHHHHHCHHHHHHHHHCCCCCEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
LRLRQTDMTKFAVGRDMALTPGKVIAQNDICQIIQYEASTETVLKRPLLICPPWINKFYI
EEEECCCCHHHHCCCCCCCCCCCEEECCHHHHHHHCCCCHHHHHHCCCEEECCCCCEEEE
LDLNPQKSFIKWCVDQGQTVFVISWVNPDARHADKDWAAYAREGIDFALETIEKATGEKD
EEECCHHHHHHHHHCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
VNAVGYCVGGTLLAATLALHAKEKNKRIKTATLFTTQVDFTHAGDLKVFVDEEQLAALEE
CCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEECEEECCCEEEEECHHHHHHHHH
HMQATGYLDGSKMSMAFNMLRASELIWPYFVNSYLKGQDPLPFDLLFWNADSTRMAAANH
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHHCCC
AFYLRNCYLRNALTRNEMILDGKPVSLKDVKIPIYNLATREDHIAPAKSVFVGSQFFGGK
HHHHHHHHHHHHHCCCCEEECCCCCCEEEEEEEEEECCCCCCCCCCHHHHEECCCCCCCE
VEFVVTGSGHIAGVVNPPDKKKYQFWTGGPAKGEYETWIDQATETPGSWWPHWRAWIETH
EEEEEECCCCEEEECCCCCCCCEEEECCCCCCCCHHHHHHHHHCCCCCCCCHHHHHEEEC
DGRRVAARKPGGDALNAIEEAPGSYVMERV
CCCEEECCCCCCHHHHHHHHCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8626293 [H]