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The map label for this gene is gcvA

Identifier: 209400092

GI number: 209400092

Start: 3768447

End: 3769364

Strand: Reverse

Name: gcvA

Synonym: ECH74115_4072

Alternate gene names: 209400092

Gene position: 3769364-3768447 (Counterclockwise)

Preceding gene: 209400704

Following gene: 209398748

Centisome position: 67.65

GC content: 49.02

Gene sequence:

>918_bases
ATGTCTAAACGATTACCACCGCTAAATGCCTTACGAGTTTTTGATGCCGCAGCACGCCATTTAAGTTTCACTCGCGCAGC
AGAAGAGCTTTTTGTGACCCAGGCCGCAGTAAGTCATCAAATCAAGTCTCTTGAGGATTTTTTGGGGCTAAAACTGTTCC
GCCGCCGTAATCGTTCACTCCTGCTGACCGAGGAAGGGCAAAGCTATTTCCTCGATATCAAAGAGATATTTTCGCAATTA
ACCGAAGCGACGCGTAAACTCCAGGCCCGTAGCGCCAAGGGGGCGTTGACGGTCAGTTTACTCCCCAGTTTCGCCATTCA
TTGGTTGGTTCCGCGACTTTCCAGCTTTAATTCAGCTTATCCGGGAATTGACGTTCGAATCCAGGCGGTTGATCGTCAGG
AAGATAAGCTGGCGGATGATGTTGATGTGGCGATATTTTATGGTCGGGGCAACTGGCCGGGGCTACGGGTGGAAAAACTG
TACGCCGAATATTTATTGCCGGTGTGTTCGCCGCTACTGCTGACTGGCGAAAAACCCTTGAAGACCCCGGAAGATCTGGC
TAAACATACGTTATTACATGATGCGTCACGCCGTGACTGGCAGACATATACTCGTCAATTGGGGTTAAATCATATCAACG
TTCAGCAAGGGCCAATTTTTAGCCATAGCGCCATGGTGCTGCAAGCGGCTATTCACGGGCAGGGAGTGGCGCTGGCAAAT
AACGTGATGGCGCAATCTGAAATTGAAGCCGGACGTCTTGTTTGCCCGTTTAATGATGTTCTGGTCAGTAAAAACGCTTT
TTATCTGGTTTGTCATGACAGCCAGGCAGAACTGGGTAAAATAGCCGCCTTTCGCCAATGGATCCTGGCGAAAGCCGCTG
CTGAACAAGAAAAATTCCGCTTTCGTTATGAACAATAA

Upstream 100 bases:

>100_bases
AGCTCAACGGACAATTTATAATGGCTCAGATTAAAAAAACTAATAGGTTACACAGTGTGATCTAATTGTTAAATTCATTT
AACATCAAAGTTTAAAAGCC

Downstream 100 bases:

>100_bases
TTTACGTAGGGTACGACCATGACCAGCCGTTTTATGCTGATTTTCGCCGCCATTAGCGGCTTCATTTTTGTGGCACTGGG
CGCTTTTGGCGCGCATGTGT

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator

Number of amino acids: Translated: 305; Mature: 304

Protein sequence:

>305_residues
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAAAEQEKFRFRYEQ

Sequences:

>Translated_305_residues
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQL
TEATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKL
YAEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAAAEQEKFRFRYEQ
>Mature_304_residues
SKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSLLLTEEGQSYFLDIKEIFSQLT
EATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFNSAYPGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLY
AEYLLPVCSPLLLTGEKPLKTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALANN
VMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAAAEQEKFRFRYEQ

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain

Homologues:

Organism=Escherichia coli, GI1789173, Length=305, Percent_Identity=100, Blast_Score=627, Evalue=0.0,
Organism=Escherichia coli, GI1786448, Length=286, Percent_Identity=34.965034965035, Blast_Score=146, Evalue=1e-36,
Organism=Escherichia coli, GI1788706, Length=288, Percent_Identity=31.25, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI145693193, Length=296, Percent_Identity=27.3648648648649, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=28.8732394366197, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI157672245, Length=212, Percent_Identity=31.1320754716981, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI87081978, Length=257, Percent_Identity=31.1284046692607, Blast_Score=81, Evalue=1e-16,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=26, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI145693105, Length=143, Percent_Identity=29.3706293706294, Blast_Score=68, Evalue=7e-13,
Organism=Escherichia coli, GI1788887, Length=176, Percent_Identity=34.0909090909091, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787128, Length=306, Percent_Identity=24.1830065359477, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789440, Length=266, Percent_Identity=26.3157894736842, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787879, Length=130, Percent_Identity=32.3076923076923, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI1790262, Length=176, Percent_Identity=28.4090909090909, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): GCVA_ECO57 (P0A9F8)

Other databases:

- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D91087
- PIR:   F85932
- RefSeq:   NP_289363.1
- RefSeq:   NP_311695.1
- ProteinModelPortal:   P0A9F8
- SMR:   P0A9F8
- EnsemblBacteria:   EBESCT00000028054
- EnsemblBacteria:   EBESCT00000055715
- GeneID:   916524
- GeneID:   958267
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z4125
- KEGG:   ecs:ECs3668
- GeneTree:   EBGT00070000031706
- HOGENOM:   HBG685425
- OMA:   FNSAYPE
- ProtClustDB:   PRK11139
- BioCyc:   ECOL83334:ECS3668-MONOMER
- GO:   GO:0005737
- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- PRINTS:   PR00039

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate

EC number: NA

Molecular weight: Translated: 34402; Mature: 34271

Theoretical pI: Translated: 9.30; Mature: 9.30

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFNSAY
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGEKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCC
KTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAAAEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FRYEQ
CCCCC
>Mature Secondary Structure 
SKRLPPLNALRVFDAAARHLSFTRAAEELFVTQAAVSHQIKSLEDFLGLKLFRRRNRSL
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEE
LLTEEGQSYFLDIKEIFSQLTEATRKLQARSAKGALTVSLLPSFAIHWLVPRLSSFNSAY
EEEECCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHCCCCC
PGIDVRIQAVDRQEDKLADDVDVAIFYGRGNWPGLRVEKLYAEYLLPVCSPLLLTGEKPL
CCCEEEEEEECCCHHHCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCHHHCCCCCCC
KTPEDLAKHTLLHDASRRDWQTYTRQLGLNHINVQQGPIFSHSAMVLQAAIHGQGVALAN
CCHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHHHCCCCCHHHH
NVMAQSEIEAGRLVCPFNDVLVSKNAFYLVCHDSQAELGKIAAFRQWILAKAAAEQEKFR
HHHHHHHCCCCCEECCCHHHEECCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHH
FRYEQ
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796