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The map label for this gene is manC2

Identifier: 209398268

GI number: 209398268

Start: 2741669

End: 2743117

Strand: Reverse

Name: manC2

Synonym: ECH74115_2965

Alternate gene names: 209398268

Gene position: 2743117-2741669 (Counterclockwise)

Preceding gene: 209397532

Following gene: 209397519

Centisome position: 49.23

GC content: 37.61

Gene sequence:

>1449_bases
ATGTCTGATGCGCCAATAATTGCTGTAGTTATGGCCGGTGGTACAGGCAGTCGTCTTTGGCCACTTTCTCGTGAACTATA
TCCAAAGCAGTTTTTACAACTCTCTGGTGATAACACCTTGTTACAAACGACTTTGCTACGACTTTCAGGCCTATCATGTC
AAAAACCATTAGTGATAACAAATGAACAGCATCGCTTTGTTGTGGCTGAACAGTTAAGGGAAATAAATAAATTAAATGGT
AATATTATTCTAGAACCATGCGGGCGAAATACTGCACCAGCAATAGCGATATCTGCGTTTCATGCGTTAAAACGTAATCC
TCAGGAAGATCCATTGCTTCTAGTTCTTGCGGCAGACCACGTTATAGCTAAAGAAAGTGTTTTCTGTGATGCTATTAAAA
ATGCAACTCCCATCGCTAATCAAGGTAAAATTGTAACGTTTGGAATTATACCAGAATATGCTGAAACTGGTTATGGGTAT
ATTGAGAGAGGTGAACTATCTGTACCGCTTCAAGGGCATGAAAATACTGGTTTTTATTATGTAAATAAGTTTGTCGAAAA
GCCTAATCGTGAAACCGCAGAATTGTATATGACTTCTGGTAATCACTATTGGAATAGTGGAATATTCATGTTTAAGGCAT
CTGTTTATCTTGAGGAATTGAGAAAATTTAGACCTGACATTTACAATGTTTGTGAACAGGTTGCCTCATCCTCATACATT
GATCTAGATTTTATTCGATTATCAAAAGAACAATTTCAAGATTGTCCTGCTGAATCTATTGATTTTGCTGTAATGGAAAA
AACAGAAAAATGTGTTGTATGCCCTGTTGATATTGGTTGGAGTGACGTTGGATCTTGGCAATCGTTATGGGACATTAGTC
TAAAATCGAAAACAGGAGATGTATGTAAAGGTGATATATTAACCTATGATACTAAGAATAATTATATCTACTCTGAGTCA
GCGTTGGTAGCCGCCATTGGAATTGAAGATATGGTTATCGTGCAAACTAAAGATGCCGTTCTTGTGTCTAAAAAGAGTGA
TGTACAGCATGTAAAAAAAATAGTCGAAATGCTTAAATTGCAGCAACGTACAGAGTATATTAGTCATCGTGAAGTTTTCC
GACCATGGGGAAAATTTGATTCGATTGACCAAGGTGAGCGATACAAAGTCAAGAAAATTATTGTGAAACCTGGTGAGGGG
CTTTCTTTAAGGATGCATCACCATCGTTCTGAACATTGGATCGTGCTTTCTGGTACAGCAAAAGTAACCCTTGGCGATAA
AACTAAACTAGTCACCGCAAATGAATCGATATACATTCCCCTTGGCGCAGCGTATAGTCTTGAGAATCCGGGCATAATCC
CTCTTAATCTTATTGAAGTCAGTTCAGGGGATTATTTGGGAGAGGATGATATTATAAGACAGAAAGAACGTTACAAACAT
GAAGATTAA

Upstream 100 bases:

>100_bases
GAGCAAAGCTGATAAATGATGACGATGTGCATTATAATTGTCGCGCATATTTTAACAATAAAACAAATGATGCGATTGGC
TTAGATAATAAGGATATAAT

Downstream 100 bases:

>100_bases
CATATGAAATCTTTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTG
GCGCATTGGGCGTGCCTATG

Product: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase 2; GMP 2; GMPP 2

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNG
NIILEPCGRNTAPAIAISAFHALKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYI
DLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSES
ALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEG
LSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKH
ED

Sequences:

>Translated_482_residues
MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNG
NIILEPCGRNTAPAIAISAFHALKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGY
IERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYI
DLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSES
ALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEG
LSLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKH
ED
>Mature_481_residues
SDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRLSGLSCQKPLVITNEQHRFVVAEQLREINKLNGN
IILEPCGRNTAPAIAISAFHALKRNPQEDPLLLVLAADHVIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGYI
ERGELSVPLQGHENTGFYYVNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYID
LDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGDVCKGDILTYDTKNNYIYSESA
LVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKLQQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGL
SLRMHHHRSEHWIVLSGTAKVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKHE
D

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family

Homologues:

Organism=Escherichia coli, GI1788362, Length=478, Percent_Identity=60.4602510460251, Blast_Score=614, Evalue=1e-177,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MANC2_ECO57 (O85342)

Other databases:

- EMBL:   AF061251
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   D90983
- PIR:   G85828
- RefSeq:   NP_288537.1
- RefSeq:   NP_310863.1
- ProteinModelPortal:   O85342
- EnsemblBacteria:   EBESCT00000026104
- EnsemblBacteria:   EBESCT00000056802
- GeneID:   912820
- GeneID:   962090
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3195
- KEGG:   ecs:ECs2836
- GeneTree:   EBGT00050000011908
- HOGENOM:   HBG422488
- OMA:   CIGLSAV
- ProtClustDB:   CLSK334800
- BioCyc:   ECOL83334:ECS2836-MONOMER
- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710
- Gene3D:   G3DSA:2.60.120.10
- TIGRFAMs:   TIGR01479

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase; SSF51182 RmlC_like_cupin

EC number: =2.7.7.13

Molecular weight: Translated: 54271; Mature: 54140

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRLSGLSCQKPLVIT
CCCCCEEEEEEECCCCCCCCCCCHHHCHHHHHEECCCCHHHHHHHHHHCCCCCCCCEEEE
NEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHALKRNPQEDPLLLVLAADH
CCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
VIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGYIERGELSVPLQGHENTGFYY
CHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHCCCCEEECCEEEEEECCCCCCCEEE
VNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYI
EEHHHCCCCCCEEEEEEECCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCE
DLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGD
EEEHEECCHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCHHHEEEEECCCCCC
VCKGDILTYDTKNNYIYSESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKL
CEECCEEEEECCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHH
QQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTA
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCEEEEECCE
KVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKH
EEEECCCEEEEEECCEEEEEECCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCC
ED
CC
>Mature Secondary Structure 
SDAPIIAVVMAGGTGSRLWPLSRELYPKQFLQLSGDNTLLQTTLLRLSGLSCQKPLVIT
CCCCEEEEEEECCCCCCCCCCCHHHCHHHHHEECCCCHHHHHHHHHHCCCCCCCCEEEE
NEQHRFVVAEQLREINKLNGNIILEPCGRNTAPAIAISAFHALKRNPQEDPLLLVLAADH
CCCCEEHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCCCCEEEEEECCC
VIAKESVFCDAIKNATPIANQGKIVTFGIIPEYAETGYGYIERGELSVPLQGHENTGFYY
CHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCHHHCCCCEEECCEEEEEECCCCCCCEEE
VNKFVEKPNRETAELYMTSGNHYWNSGIFMFKASVYLEELRKFRPDIYNVCEQVASSSYI
EEHHHCCCCCCEEEEEEECCCEEECCCEEEEEHHHHHHHHHHHCCHHHHHHHHHHCCCCE
DLDFIRLSKEQFQDCPAESIDFAVMEKTEKCVVCPVDIGWSDVGSWQSLWDISLKSKTGD
EEEHEECCHHHHHCCCCCCCCCCEEECCCCEEEEEECCCCCCCCCCHHHEEEEECCCCCC
VCKGDILTYDTKNNYIYSESALVAAIGIEDMVIVQTKDAVLVSKKSDVQHVKKIVEMLKL
CEECCEEEEECCCCEEEECCCEEEEECCCEEEEEEECCEEEEECCHHHHHHHHHHHHHHH
QQRTEYISHREVFRPWGKFDSIDQGERYKVKKIIVKPGEGLSLRMHHHRSEHWIVLSGTA
HHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEECCCCCCEEEEECCCCCCEEEEECCE
KVTLGDKTKLVTANESIYIPLGAAYSLENPGIIPLNLIEVSSGDYLGEDDIIRQKERYKH
EEEECCCEEEEEECCEEEEEECCEEECCCCCEEEEEEEEECCCCCCCCHHHHHHHHHCCC
ED
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 11206551; 11258796