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The map label for this gene is manB

Identifier: 209397519

GI number: 209397519

Start: 2740295

End: 2741665

Strand: Reverse

Name: manB

Synonym: ECH74115_2964

Alternate gene names: 209397519

Gene position: 2741665-2740295 (Counterclockwise)

Preceding gene: 209398268

Following gene: 209400871

Centisome position: 49.2

GC content: 54.12

Gene sequence:

>1371_bases
ATGAAATCTTTAACCTGCTTTAAAGCCTATGATATTCGCGGGAAATTAGGCGAAGAACTGAATGAAGATATTGCCTGGCG
CATTGGGCGTGCCTATGGCGAATTTCTCAAACCGAAAACCATTGTTTTAGGCGGTGATGTCCGCCTCACCAGCGAAGCGT
TAAAACTGGCGCTTGCGAAAGGTTTACAGGATGCGGGCGTCGATGTGCTGGATATCGGTATGTCCGGCACCGAAGAGATC
TATTTCGCCACGTTCCATCTCGGAGTGGATGGCGGCATCGAAGTTACCGCCAGCCATAACCCGATGGATTACAACGGCAT
GAAGCTGGTGCGCGAAGGGGCTCGCCCGATCAGCGGTGATACCGGACTGCGCGATGTCCAGCGTCTGGCAGAAGCCAATG
ACTTCCCTCCTGTCGATGAAACCAAACGTGGTCGCTATCAGCAAATCAATCTGCGTGACGCTTACGTTGATCACCTGTTC
GGTTATATCAACGTCAAAAACCTCACGCCGCTCAAGCTGGTGATCAACTCCGGGAACGGCGCAGCGGGTCCGGTGGTGGA
CGCCATTGAAGCCCGATTTAAAGCCCTCGGCGCACCGGTGGAATTAATCAAAGTACACAACACGCCGGACGGCAATTTCC
CCAACGGTATTCCTAACCCGCTGCTGCCGGAATGCCGCGACGACACCCGTAATGCGGTCATCAAACACGGCGCGGATATG
GGCATTGCCTTTGATGGCGATTTTGACCGCTGTTTCCTGTTTGACGAAAAAGGGCAGTTTATCGAGGGCTACTACATTGT
CGGCCTGCTGGCAGAAGCGTTCCTCGAAAAAAATCCCGGCGCGAAGATCATCCACGATCCACGTCTCTCCTGGAACACCG
TTGATGTGGTGACTGCCGCAGGCGGCACCCCGGTAATGTCGAAAACCGGACACGCCTTTATTAAAGAACGTATGCGCAAG
GAAGACGCCATCTACGGTGGCGAAATGAGCGCTCACCATTACTTCCGTGATTTCGCTTACTGCGACAGCGGCATGATCCC
GTGGCTGCTGGTCGCCGAACTGGTGTGCCTGAAAGGAAAAACGCTGGGCGAAATGGTGCGCGACCGGATGGCGGCGTTTC
CGGCAAGCGGTGAGATCAACAGCAAACTGGCGCAACCCGTTGAGGCAATTAATCGCGTGGAACAGCATTTTAGCCGCGAG
GCGCTGGCGGTGGATCGCACCGATGGCATCAGCATGACCTTTGCCGACTGGCGCTTTAACCTGCGCTCCTCCAACACCGA
ACCGGTGGTGCGGTTGAATGTGGAATCACGCGGTGATGTAAAGCTAATGGAAAAGAAAACTAAAGCTCTTCTTAAATTGC
TAAGTGAGTGA

Upstream 100 bases:

>100_bases
CATAATCCCTCTTAATCTTATTGAAGTCAGTTCAGGGGATTATTTGGGAGAGGATGATATTATAAGACAGAAAGAACGTT
ACAAACATGAAGATTAACAT

Downstream 100 bases:

>100_bases
TTATTTACATTAATCATTAAGCGTATTTAAGATTATATTAAAGTAATGTTATTGCGGTATATGATGAATATGTGGGCTTT
TTTATGTATAACGACTATAC

Product: phosphomannomutase

Products: NA

Alternate protein names: PMM

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE

Sequences:

>Translated_456_residues
MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE
>Mature_456_residues
MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSEALKLALAKGLQDAGVDVLDIGMSGTEEI
YFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGDTGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLF
GYINVKNLTPLKLVINSGNGAAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAAGGTPVMSKTGHAFIKERMRK
EDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGKTLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSRE
ALAVDRTDGISMTFADWRFNLRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE

Specific function: Involved in GDP-mannose biosynthesis which serves as the activated sugar nucleotide precursor for mannose residues in cell surface polysaccharides. This enzyme participates in synthesis of the LPS O antigen

COG id: COG1109

COG function: function code G; Phosphomannomutase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphohexose mutase family

Homologues:

Organism=Escherichia coli, GI1788361, Length=456, Percent_Identity=97.3684210526316, Blast_Score=918, Evalue=0.0,
Organism=Escherichia coli, GI1789566, Length=460, Percent_Identity=25.8695652173913, Blast_Score=84, Evalue=1e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MANB_ECO57 (O85343)

Other databases:

- EMBL:   AF061251
- EMBL:   AB008676
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   C90983
- PIR:   F85828
- RefSeq:   NP_288536.1
- RefSeq:   NP_310862.1
- ProteinModelPortal:   O85343
- SMR:   O85343
- EnsemblBacteria:   EBESCT00000027179
- EnsemblBacteria:   EBESCT00000057419
- GeneID:   913941
- GeneID:   962089
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3194
- KEGG:   ecs:ECs2835
- GeneTree:   EBGT00050000008225
- HOGENOM:   HBG644964
- OMA:   ENECRIE
- ProtClustDB:   PRK15414
- BioCyc:   ECOL83334:ECS2835-MONOMER
- InterPro:   IPR005844
- InterPro:   IPR016055
- InterPro:   IPR005845
- InterPro:   IPR005846
- InterPro:   IPR005843
- InterPro:   IPR016066
- InterPro:   IPR005841
- Gene3D:   G3DSA:3.40.120.10
- PRINTS:   PR00509

Pfam domain/function: PF02878 PGM_PMM_I; PF02879 PGM_PMM_II; PF02880 PGM_PMM_III; PF00408 PGM_PMM_IV; SSF53738 A-D-PHexomutase_a/b/a-I/II/III

EC number: =5.4.2.8

Molecular weight: Translated: 50340; Mature: 50340

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00710 PGM_PMM

Important sites: ACT_SITE 98-98

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSEALKLALAK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
CEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
TLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCEEEEECEEEE
LRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSLTCFKAYDIRGKLGEELNEDIAWRIGRAYGEFLKPKTIVLGGDVRLTSEALKLALAK
CCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEHHHHHHHHHHH
GLQDAGVDVLDIGMSGTEEIYFATFHLGVDGGIEVTASHNPMDYNGMKLVREGARPISGD
CHHHCCCCEEEECCCCCCEEEEEEEEEECCCCEEEEECCCCCCCCHHHHHHHCCCCCCCC
TGLRDVQRLAEANDFPPVDETKRGRYQQINLRDAYVDHLFGYINVKNLTPLKLVINSGNG
CCHHHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHHEEEECCCCEEEEEEECCCC
AAGPVVDAIEARFKALGAPVELIKVHNTPDGNFPNGIPNPLLPECRDDTRNAVIKHGADM
CCCHHHHHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCC
GIAFDGDFDRCFLFDEKGQFIEGYYIVGLLAEAFLEKNPGAKIIHDPRLSWNTVDVVTAA
CEEECCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHCCCCCCEEECCCCCCCEEEEEEEC
GGTPVMSKTGHAFIKERMRKEDAIYGGEMSAHHYFRDFAYCDSGMIPWLLVAELVCLKGK
CCCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCC
TLGEMVRDRMAAFPASGEINSKLAQPVEAINRVEQHFSREALAVDRTDGISMTFADWRFN
HHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCHHHEEEECCCCCEEEEECEEEE
LRSSNTEPVVRLNVESRGDVKLMEKKTKALLKLLSE
ECCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9673232; 10222209; 11206551; 11258796