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The map label for this gene is wbdQ

Identifier: 209397532

GI number: 209397532

Start: 2743099

End: 2743608

Strand: Reverse

Name: wbdQ

Synonym: ECH74115_2966

Alternate gene names: 209397532

Gene position: 2743608-2743099 (Counterclockwise)

Preceding gene: 209397079

Following gene: 209398268

Centisome position: 49.24

GC content: 38.04

Gene sequence:

>510_bases
ATGTTTTTACATTCCCAAGACTTTGCCACAATTGTAAGGTCTACTCCTCTTATTTCTATAGATTTGATTGTGGAAAACGA
GTTTGGCGAAATTTTGCTAGGAAAACGAATCAACCGCCCGGCACAGGGCTATTGGTTCGTTCCTGGTGGTAGGGTGTTGA
AAGATGAAAAATTGCAGACAGCCTTTGAACGATTGACAGAAATTGAACTAGGAATTCGTTTGCCTCTCTCTGTGGGTAAG
TTTTATGGTATCTGGCAGCACTTCTACGAAGACAATAGTATGGGGGGAGACTTTTCAACGCATTATATAGTTATAGCATT
CCTTCTTAAATTACAACCAAACATTTTGAAATTACCGAAGTCACAACATAATGCTTATTGCTGGCTATCGCGAGCAAAGC
TGATAAATGATGACGATGTGCATTATAATTGTCGCGCATATTTTAACAATAAAACAAATGATGCGATTGGCTTAGATAAT
AAGGATATAATATGTCTGATGCGCCAATAA

Upstream 100 bases:

>100_bases
TTCATCAACTAGGTTGGAATCATAAAATTACCCTTCACAAGGGTCTTGAAAATACATACAACTGGTTTCTTGAAAACCAA
CTTCAATATCGGGGGTAATA

Downstream 100 bases:

>100_bases
TTGCTGTAGTTATGGCCGGTGGTACAGGCAGTCGTCTTTGGCCACTTTCTCGTGAACTATATCCAAAGCAGTTTTTACAA
CTCTCTGGTGATAACACCTT

Product: gdp-mannose mannosyl hydrolase

Products: GDP; mannose [C]

Alternate protein names: GDPMH

Number of amino acids: Translated: 169; Mature: 169

Protein sequence:

>169_residues
MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGYWFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGK
FYGIWQHFYEDNSMGGDFSTHYIVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
KDIICLMRQ

Sequences:

>Translated_169_residues
MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGYWFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGK
FYGIWQHFYEDNSMGGDFSTHYIVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
KDIICLMRQ
>Mature_169_residues
MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGYWFVPGGRVLKDEKLQTAFERLTEIELGIRLPLSVGK
FYGIWQHFYEDNSMGGDFSTHYIVIAFLLKLQPNILKLPKSQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDN
KDIICLMRQ

Specific function: It could participate in the regulation of cell wall biosynthesis by influencing the concentration of GDP-mannose or GDP-glucose in the cell. May be involved in the degradation of GDP-mannose and GDP-glucose, diverting the GDP to the synthesis of GDP-fucos

COG id: COG0494

COG function: function code LR; NTP pyrophosphohydrolases including oxidative damage repair enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain

Homologues:

Organism=Escherichia coli, GI87082031, Length=148, Percent_Identity=62.8378378378378, Blast_Score=210, Evalue=4e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): WBDQ_ECO57 (O85341)

Other databases:

- EMBL:   AF061251
- EMBL:   AB008676
- EMBL:   AE005174
- EMBL:   BA000007
- PIR:   E90983
- PIR:   H85828
- RefSeq:   NP_288538.1
- RefSeq:   NP_310864.1
- ProteinModelPortal:   O85341
- SMR:   O85341
- EnsemblBacteria:   EBESCT00000025759
- EnsemblBacteria:   EBESCT00000057167
- GeneID:   912421
- GeneID:   962091
- GenomeReviews:   AE005174_GR
- GenomeReviews:   BA000007_GR
- KEGG:   ece:Z3196
- KEGG:   ecs:ECs2837
- GeneTree:   EBGT00050000010110
- HOGENOM:   HBG373807
- OMA:   THYIVLA
- ProtClustDB:   PRK15434
- BioCyc:   ECOL83334:ECS2837-MONOMER
- InterPro:   IPR021161
- InterPro:   IPR000086
- InterPro:   IPR015797
- Gene3D:   G3DSA:3.90.79.10
- PIRSF:   PIRSF037599

Pfam domain/function: PF00293 NUDIX; SSF55811 NUDIX_hydrolase

EC number: NA

Molecular weight: Translated: 19568; Mature: 19568

Theoretical pI: Translated: 7.55; Mature: 7.55

Prosite motif: PS51462 NUDIX; PS00893 NUDIX_BOX

Important sites: BINDING 8-8 BINDING 51-51 BINDING 64-64 BINDING 102-102

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGYWFVPGGRVLKDEKLQT
CCCCCCHHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCEECHHHHHH
AFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHYIVIAFLLKLQPNILKLPK
HHHHHHHHHCCEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCC
SQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDNKDIICLMRQ
CCCCEEEEEEECEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECC
>Mature Secondary Structure
MFLHSQDFATIVRSTPLISIDLIVENEFGEILLGKRINRPAQGYWFVPGGRVLKDEKLQT
CCCCCCHHHHHHHCCCEEEEEEEEECCCCCEEECCCCCCCCCCEEECCCCCEECHHHHHH
AFERLTEIELGIRLPLSVGKFYGIWQHFYEDNSMGGDFSTHYIVIAFLLKLQPNILKLPK
HHHHHHHHHCCEECCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCEECCC
SQHNAYCWLSRAKLINDDDVHYNCRAYFNNKTNDAIGLDNKDIICLMRQ
CCCCEEEEEEECEECCCCCCEEEEEEEECCCCCCCCCCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: GDP-mannose; H2O [C]

Specific reaction: GDP-mannose + H2O = GDP + mannose [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9673232; 10222209; 11206551; 11258796