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The map label for this gene is rbsB [H]

Identifier: 209397860

GI number: 209397860

Start: 5238089

End: 5239012

Strand: Reverse

Name: rbsB [H]

Synonym: ECH74115_5599

Alternate gene names: 209397860

Gene position: 5239012-5238089 (Counterclockwise)

Preceding gene: 209400838

Following gene: 209397336

Centisome position: 94.02

GC content: 56.49

Gene sequence:

>924_bases
GTGACCGCGCTCTGTGCAGGCGCGCTGCTGGCCGCAGCACCGTTCGCCCAGGCGAAAGAACTGAAATCAATAGGCGTAAC
GGTGGGCGATCTGGCGAACCCGTTCTTCGTGCAGATCACCAAAGGCGCGGAGCTGGAAGCACGCAAACTGGCAGGCGATA
ACGTCAAAGTGACGCTGGTTTCCAGCGGCTACGATCTGGGTCAGCAGGTGTCGCAGATAGACAACTTTATCGCCGCTAAC
GTCGATATGATTATCCTCAACGCTGCCGATTCAAAAGGGATCGGCCCGGCGGTGAAACGGGCGAAAGATGCCGGGATCGT
GGTCGTCGCGGTTGACGTAGCGGCAGAAGGTGCCGATGCCACCATCACTTCCGATAACACCCAGGCAGGCGAAATGGCCT
GTAAATACATCACCGATCGCCTGAAAGGCAAAGGCAACGTGGTGATCATCAACGGCCCGCCGGTATCCGCCGTGCAAAAC
CGCGTCGAAGGATGCCAGACTGAATTTAAACGCCATCCTGACATCAAAGTGCTCTCTGACAACCAGAACGCGAAAGGCAG
TCGTGAAGGCGGGCTGGAGGTAATGACGGGACTGCTGGCGGCAAATCCGAAGATCGACGGCGTATTTGCGATTAACGATC
CCACCGCAATCGGTGCCGATCTGGCAGCAAAACAGGCACAACGTAACGAGTTCTTTATTGTCGGTGTGGACGGAAGCCCG
GACGCCGAAGAGGCGCTCAAGCGTGAGAACTCGTTGTTTGTCGCTACTCCGGCGCAAGATCCCCAGGTGATGGCAGCGAA
AGCGGTGGAAATTGGCTACGACATTTTGCAGGGCAAACCTGCGCCGAAAGAGCCGGTGCTGATCCCGGTGACGATGATCG
ATAAAAACAACGTCGGCAATTACAAAGGCTGGACGGTGAAATAA

Upstream 100 bases:

>100_bases
GGCGATGATCATCATTGCGGTGCTGATCGACAAATACCGTACCCGACACCATCAAAGTGCATAAACACATGAGGAATACA
GCATGAGATTGAAGCCCTTA

Downstream 100 bases:

>100_bases
GTCCACACGCCGGGCGGCGCAGCTGCCCGGTCACCGCTAAGGAGAACGCTTTCGATGAAACGCTCCGCTATAAACGACAT
TCTCGGCCATACACGGCAAT

Product: periplasmic binding protein/LacI transcriptional regulator

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 307; Mature: 306

Protein sequence:

>307_residues
MTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAAN
VDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPPVSAVQN
RVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAINDPTAIGADLAAKQAQRNEFFIVGVDGSP
DAEEALKRENSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK

Sequences:

>Translated_307_residues
MTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAAN
VDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPPVSAVQN
RVEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAINDPTAIGADLAAKQAQRNEFFIVGVDGSP
DAEEALKRENSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK
>Mature_306_residues
TALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQITKGAELEARKLAGDNVKVTLVSSGYDLGQQVSQIDNFIAANV
DMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADATITSDNTQAGEMACKYITDRLKGKGNVVIINGPPVSAVQNR
VEGCQTEFKRHPDIKVLSDNQNAKGSREGGLEVMTGLLAANPKIDGVFAINDPTAIGADLAAKQAQRNEFFIVGVDGSPD
AEEALKRENSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPKEPVLIPVTMIDKNNVGNYKGWTVK

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=290, Percent_Identity=34.1379310344828, Blast_Score=121, Evalue=6e-29,
Organism=Escherichia coli, GI1790526, Length=280, Percent_Identity=29.6428571428571, Blast_Score=89, Evalue=5e-19,
Organism=Escherichia coli, GI1790715, Length=293, Percent_Identity=26.6211604095563, Blast_Score=74, Evalue=8e-15,
Organism=Escherichia coli, GI1789990, Length=211, Percent_Identity=32.7014218009479, Blast_Score=68, Evalue=9e-13,
Organism=Escherichia coli, GI1790674, Length=191, Percent_Identity=30.3664921465969, Blast_Score=65, Evalue=4e-12,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 32082; Mature: 31951

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQITKGAELEARKLAGDNVKVTLV
CCCHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCHHHHCCCCEEEEEE
SSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADA
ECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCE
TITSDNTQAGEMACKYITDRLKGKGNVVIINGPPVSAVQNRVEGCQTEFKRHPDIKVLSD
EEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
NQNAKGSREGGLEVMTGLLAANPKIDGVFAINDPTAIGADLAAKQAQRNEFFIVGVDGSP
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCC
DAEEALKRENSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPKEPVLIPVTMIDKNNVGN
CHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCC
YKGWTVK
CCCCEEC
>Mature Secondary Structure 
TALCAGALLAAAPFAQAKELKSIGVTVGDLANPFFVQITKGAELEARKLAGDNVKVTLV
CCHHHHHHHHHCCHHHHHHHHHCCCCHHHCCCCEEEEEECCCCCCHHHHCCCCEEEEEE
SSGYDLGQQVSQIDNFIAANVDMIILNAADSKGIGPAVKRAKDAGIVVVAVDVAAEGADA
ECCCCHHHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHHCCCCEEEEEEEEECCCCCE
TITSDNTQAGEMACKYITDRLKGKGNVVIINGPPVSAVQNRVEGCQTEFKRHPDIKVLSD
EEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHCCCCEEEEEC
NQNAKGSREGGLEVMTGLLAANPKIDGVFAINDPTAIGADLAAKQAQRNEFFIVGVDGSP
CCCCCCCCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHCCCCEEEEEECCCC
DAEEALKRENSLFVATPAQDPQVMAAKAVEIGYDILQGKPAPKEPVLIPVTMIDKNNVGN
CHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEEECCCCCC
YKGWTVK
CCCCEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]