Click here to switch to the map view.

The map label for this gene is fcl [H]

Identifier: 209397079

GI number: 209397079

Start: 2743611

End: 2744576

Strand: Reverse

Name: fcl [H]

Synonym: ECH74115_2967

Alternate gene names: 209397079

Gene position: 2744576-2743611 (Counterclockwise)

Preceding gene: 209400350

Following gene: 209397532

Centisome position: 49.26

GC content: 38.61

Gene sequence:

>966_bases
ATGAATAAGCAACGTATTTTTATTGCTGGTCACCAAGGAATGGTTGGATCAGCTATTACCCGACGCCTCAAACAACGTGA
TGATGTTGAGTTGGTTTTACGTACTCGGGATGAATTGAACTTGTTGGATAGTAGCGCTGTTTTGGATTTTTTTTCTTCAC
AGAAAATCGACCAGGTTTATTTGGCAGCAGCAAAAGTCGGAGGTATTTTAGCTAACAGTTCTTATCCTGCCGATTTTATA
TATGAGAATATAATGATAGAGGCGAATGTCATTCATGCTGCCCACAAAAATAATGTAAATAAACTGCTTTTCCTCGGTTC
GTCGTGTATTTATCCTAAGTTAGCACACCAACCGATTATGGAAGACGAATTATTACAAGGGAAACTTGAGCCAACAAATG
AACCTTATGCTATCGCAAAAATTGCAGGTATTAAATTATGTGAATCTTATAACCGTCAGTTTGGGCGTGATTACCGTTCA
GTAATGCCAACCAATCTTTATGGTCCAAATGACAATTTTCATCCAAGTAATTCTCATGTGATTCCGGCGCTTTTGCGCCG
CTTTCATGATGCTGTGGAAAACAATTCTCCGAATGTTGTTGTTTGGGGAAGTGGTACTCCAAAGCGTGAATTCTTACATG
TAGATGATATGGCTTCTGCAAGCATTTATGTCATGGAGATGCCATACGATATATGGCAAAAAAATACTAAAGTAATGTTG
TCTCATATCAATATTGGAACAGGTATTGACTGCACGATTTGTGAGCTTGCGGAAACAATAGCAAAAGTTGTAGGTTATAA
AGGGCATATTACGTTCGATACAACAAAGCCCGATGGAGCCCCTCGAAAACTACTTGATGTAACGCTTCTTCATCAACTAG
GTTGGAATCATAAAATTACCCTTCACAAGGGTCTTGAAAATACATACAACTGGTTTCTTGAAAACCAACTTCAATATCGG
GGGTAA

Upstream 100 bases:

>100_bases
AAATGATTTCTGAAATGGTTGCCAAAGATCTTGAAGCCGCTAAAAAACATTCTCTTTTAAAATCGCATGGTTTTTCTGTA
AGCTTAGCTCTGGAATGATG

Downstream 100 bases:

>100_bases
TAATGTTTTTACATTCCCAAGACTTTGCCACAATTGTAAGGTCTACTCCTCTTATTTCTATAGATTTGATTGTGGAAAAC
GAGTTTGGCGAAATTTTGCT

Product: GDP-L-fucose synthetase

Products: NA

Alternate protein names: GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDSSAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFI
YENIMIEANVIHAAHKNNVNKLLFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVML
SHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYR
G

Sequences:

>Translated_321_residues
MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDSSAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFI
YENIMIEANVIHAAHKNNVNKLLFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVML
SHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYR
G
>Mature_321_residues
MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDSSAVLDFFSSQKIDQVYLAAAKVGGILANSSYPADFI
YENIMIEANVIHAAHKNNVNKLLFLGSSCIYPKLAHQPIMEDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRS
VMPTNLYGPNDNFHPSNSHVIPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVML
SHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKITLHKGLENTYNWFLENQLQYR
G

Specific function: Two step NADP-dependent conversion of GDP-4-dehydro-6- deoxy-D-mannose to GDP-fucose, involving an epimerase and a reductase reaction [H]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fucose synthase family [H]

Homologues:

Organism=Homo sapiens, GI4507709, Length=320, Percent_Identity=29.6875, Blast_Score=152, Evalue=4e-37,
Organism=Homo sapiens, GI7657641, Length=285, Percent_Identity=25.2631578947368, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI42516563, Length=344, Percent_Identity=24.1279069767442, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788365, Length=321, Percent_Identity=78.816199376947, Blast_Score=553, Evalue=1e-159,
Organism=Escherichia coli, GI1788353, Length=355, Percent_Identity=23.6619718309859, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI48994969, Length=360, Percent_Identity=21.9444444444444, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17554428, Length=316, Percent_Identity=29.4303797468354, Blast_Score=142, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19922778, Length=326, Percent_Identity=29.4478527607362, Blast_Score=154, Evalue=6e-38,
Organism=Drosophila melanogaster, GI21356223, Length=337, Percent_Identity=26.1127596439169, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI19923002, Length=294, Percent_Identity=22.108843537415, Blast_Score=65, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =1.1.1.271 [H]

Molecular weight: Translated: 36269; Mature: 36269

Theoretical pI: Translated: 7.42; Mature: 7.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDSSAVLDFFSSQKIDQVY
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHH
LAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKLLFLGSSCIYPKLAHQPIM
HHHHHHCCEEECCCCCHHHHHHHEEEEEEEEEEHHCCCCCEEEEECCCCCCCHHHCCCCC
EDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRSVMPTNLYGPNDNFHPSNSHV
CHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVML
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEEEEECCHHHHCCCCEEEE
SHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKIT
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEE
LHKGLENTYNWFLENQLQYRG
EECCCCHHHHHHHCCCEECCC
>Mature Secondary Structure
MNKQRIFIAGHQGMVGSAITRRLKQRDDVELVLRTRDELNLLDSSAVLDFFSSQKIDQVY
CCCCEEEEECCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHHHHCCCHHHHH
LAAAKVGGILANSSYPADFIYENIMIEANVIHAAHKNNVNKLLFLGSSCIYPKLAHQPIM
HHHHHHCCEEECCCCCHHHHHHHEEEEEEEEEEHHCCCCCEEEEECCCCCCCHHHCCCCC
EDELLQGKLEPTNEPYAIAKIAGIKLCESYNRQFGRDYRSVMPTNLYGPNDNFHPSNSHV
CHHHHCCCCCCCCCCEEEHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCCCCCCCCCCCCC
IPALLRRFHDAVENNSPNVVVWGSGTPKREFLHVDDMASASIYVMEMPYDIWQKNTKVML
HHHHHHHHHHHHHCCCCCEEEECCCCCCCCEEEECCCCCCEEEEEECCHHHHCCCCEEEE
SHINIGTGIDCTICELAETIAKVVGYKGHITFDTTKPDGAPRKLLDVTLLHQLGWNHKIT
EEECCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEE
LHKGLENTYNWFLENQLQYRG
EECCCCHHHHHHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7815923; 8759852; 9097040; 9278503; 9473059; 9862812 [H]