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The map label for this gene is MetK [H]

Identifier: 20806993

GI number: 20806993

Start: 500405

End: 501592

Strand: Direct

Name: MetK [H]

Synonym: TTE0488

Alternate gene names: 20806993

Gene position: 500405-501592 (Clockwise)

Preceding gene: 20806992

Following gene: 20806994

Centisome position: 18.61

GC content: 45.29

Gene sequence:

>1188_bases
ATGCGTAGACTCTTTACTTCTGAGTCAGTCACTGAAGGGCATCCTGACAAGATCTGTGACCAGATTTCAGATGCCATTTT
GGATGAAATTTTAAAAAAAGACCCTTACGCCCGCGTGGCATGTGAGACAGCTGTAACTACCGGCTTGGTTCTGGTAATGG
GAGAAATTACTACAGAGTGCTATGTGGACATTCCCAGGATAGCCAGAGACGTCATAAGGGATATAGGCTATACCAGAGCA
AAATACGGCTTTGACGCAGACACCTGTGCTGTAATCACTTCCATTGACGAGCAGTCTCCAGACATTGCCATGGGAGTGAA
TAAGGCTTTAGAAGCAAGAAGAGGAGAGCTGACAGATGCCGAAATCGAGGCAATTGGCGCTGGAGACCAGGGCCTCATGA
TAGGCTTTGCCTGCGATGAGACAGAAGAGCTCATGCCAATGCCCATAATGCTGGCTCACAAGCTGGCCAGAAGATTGGCT
GAGGTCAGGAAAAACGGCACATTGAGCTATCTAAGACCTGATGGAAAAACTCAGGTGACAGTAGAGTACGAGGAAGACAG
GCCTGTCAGAGTCGACTCTGTTGTGGTTTCAGCTCAGCATGCTCCTGAGGTTGACCATGACACGATAGAAAAAGACATAA
TTGAACATGTGGTAAATGTGATTATTCCTGAAAACATGATGGACAAGAACACTAAAATTTTTGTGAATCCTACTGGCAGG
TTTGTGCTAGGAGGTCCCCAAGCAGATAGCGGCCTTACAGGGAGAAAGATCATAGTAGATACCTATGGTGGATACGCAAG
GCATGGAGGCGGCGCATTTTCAGGGAAAGACCCAACAAAAGTTGACAGGTCAGCAAGTTATGCGGCAAGGTATGTGGCTA
AAAACATCGTAGCGGCAGGTCTCGCTAAGAAATGTGAAGTACAGGTGGCGTATGCAATAGGTGTGGCAACACCACTGGAG
GTTGAGATAAACACATTTGGGACAGGCAAAATTTCTGATGAAAAAATATCAGAAATTGTAAAGAAAGTTTTTGATTTAAG
GCCTGCAGCCATAATAAGAGATTTGGATTTGAGAAGACCTATTTATAGGCAGGTTGCTGCTTATGGCCACTTTGGAAGAC
ATGACTTGGACCTGCCGTGGGAGAAAACAGATAGAGTAGATATATTGAGAAAATTAGCAGGAATATAG

Upstream 100 bases:

>100_bases
ATAAGGTGCCAATTCTCTCAGAAGATTTTTTCTTCTGAAAGATGAGGGTATGCCTCTTCTTTTAGAAGGGGTTTTATTTT
GCTCTTAAGGAGGGAAGAAG

Downstream 100 bases:

>100_bases
TTAAAGCCCGGGTTTTGCCCGGGCTTGTGGCGTTTTTTATATATTTTTTGTAACGCCAGAGATTTTTAAAACCGCTCCGC
TAAAATATATTATACATTAG

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 395; Mature: 395

Protein sequence:

>395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTECYVDIPRIARDVIRDIGYTRA
KYGFDADTCAVITSIDEQSPDIAMGVNKALEARRGELTDAEIEAIGAGDQGLMIGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEEDRPVRVDSVVVSAQHAPEVDHDTIEKDIIEHVVNVIIPENMMDKNTKIFVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQVAYAIGVATPLE
VEINTFGTGKISDEKISEIVKKVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDRVDILRKLAGI

Sequences:

>Translated_395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTECYVDIPRIARDVIRDIGYTRA
KYGFDADTCAVITSIDEQSPDIAMGVNKALEARRGELTDAEIEAIGAGDQGLMIGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEEDRPVRVDSVVVSAQHAPEVDHDTIEKDIIEHVVNVIIPENMMDKNTKIFVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQVAYAIGVATPLE
VEINTFGTGKISDEKISEIVKKVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDRVDILRKLAGI
>Mature_395_residues
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTECYVDIPRIARDVIRDIGYTRA
KYGFDADTCAVITSIDEQSPDIAMGVNKALEARRGELTDAEIEAIGAGDQGLMIGFACDETEELMPMPIMLAHKLARRLA
EVRKNGTLSYLRPDGKTQVTVEYEEDRPVRVDSVVVSAQHAPEVDHDTIEKDIIEHVVNVIIPENMMDKNTKIFVNPTGR
FVLGGPQADSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAGLAKKCEVQVAYAIGVATPLE
VEINTFGTGKISDEKISEIVKKVFDLRPAAIIRDLDLRRPIYRQVAAYGHFGRHDLDLPWEKTDRVDILRKLAGI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=382, Percent_Identity=56.5445026178011, Blast_Score=424, Evalue=1e-119,
Organism=Homo sapiens, GI4557737, Length=382, Percent_Identity=54.4502617801047, Blast_Score=414, Evalue=1e-116,
Organism=Escherichia coli, GI1789311, Length=394, Percent_Identity=58.6294416243655, Blast_Score=490, Evalue=1e-140,
Organism=Caenorhabditis elegans, GI17538498, Length=391, Percent_Identity=53.1969309462916, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17538494, Length=401, Percent_Identity=51.8703241895262, Blast_Score=421, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI25145633, Length=395, Percent_Identity=52.6582278481013, Blast_Score=420, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17551082, Length=391, Percent_Identity=51.6624040920716, Blast_Score=407, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI32565905, Length=401, Percent_Identity=47.6309226932668, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI32565911, Length=344, Percent_Identity=50.8720930232558, Blast_Score=350, Evalue=7e-97,
Organism=Caenorhabditis elegans, GI25145635, Length=348, Percent_Identity=50.2873563218391, Blast_Score=348, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI32565903, Length=327, Percent_Identity=50.1529051987768, Blast_Score=324, Evalue=4e-89,
Organism=Caenorhabditis elegans, GI32565909, Length=83, Percent_Identity=55.421686746988, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI32565907, Length=38, Percent_Identity=76.3157894736842, Blast_Score=70, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6323209, Length=383, Percent_Identity=55.3524804177546, Blast_Score=430, Evalue=1e-121,
Organism=Saccharomyces cerevisiae, GI6320710, Length=389, Percent_Identity=54.2416452442159, Blast_Score=429, Evalue=1e-121,
Organism=Drosophila melanogaster, GI45552159, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580529, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580537, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580533, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580535, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580531, Length=389, Percent_Identity=52.6992287917738, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24580527, Length=389, Percent_Identity=51.6709511568123, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580525, Length=389, Percent_Identity=51.6709511568123, Blast_Score=387, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580541, Length=377, Percent_Identity=50.3978779840849, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24580539, Length=127, Percent_Identity=51.1811023622047, Blast_Score=130, Evalue=1e-30,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 43461; Mature: 43461

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTEC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVDIPRIARDVIRDIGYTRAKYGFDADTCAVITSIDEQSPDIAMGVNKALEARRGELTDA
HCCCHHHHHHHHHHCCCHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
EIEAIGAGDQGLMIGFACDETEELMPMPIMLAHKLARRLAEVRKNGTLSYLRPDGKTQVT
CEEEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
VEYEEDRPVRVDSVVVSAQHAPEVDHDTIEKDIIEHVVNVIIPENMMDKNTKIFVNPTGR
EEECCCCCEEEHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
FVLGGPQADSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAG
EEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAKKCEVQVAYAIGVATPLEVEINTFGTGKISDEKISEIVKKVFDLRPAAIIRDLDLRRP
CHHHCEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCH
IYRQVAAYGHFGRHDLDLPWEKTDRVDILRKLAGI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC
>Mature Secondary Structure
MRRLFTSESVTEGHPDKICDQISDAILDEILKKDPYARVACETAVTTGLVLVMGEITTEC
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
YVDIPRIARDVIRDIGYTRAKYGFDADTCAVITSIDEQSPDIAMGVNKALEARRGELTDA
HCCCHHHHHHHHHHCCCHHHHCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHCCCCCCC
EIEAIGAGDQGLMIGFACDETEELMPMPIMLAHKLARRLAEVRKNGTLSYLRPDGKTQVT
CEEEECCCCCCEEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEE
VEYEEDRPVRVDSVVVSAQHAPEVDHDTIEKDIIEHVVNVIIPENMMDKNTKIFVNPTGR
EEECCCCCEEEHHEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCC
FVLGGPQADSGLTGRKIIVDTYGGYARHGGGAFSGKDPTKVDRSASYAARYVAKNIVAAG
EEECCCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
LAKKCEVQVAYAIGVATPLEVEINTFGTGKISDEKISEIVKKVFDLRPAAIIRDLDLRRP
CHHHCEEEEEEEEECCCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCH
IYRQVAAYGHFGRHDLDLPWEKTDRVDILRKLAGI
HHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA