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The map label for this gene is AcnA [H]

Identifier: 20806979

GI number: 20806979

Start: 485665

End: 487590

Strand: Direct

Name: AcnA [H]

Synonym: TTE0473

Alternate gene names: 20806979

Gene position: 485665-487590 (Clockwise)

Preceding gene: 20806978

Following gene: 20806983

Centisome position: 18.06

GC content: 42.99

Gene sequence:

>1926_bases
ATGAATTTGACGCAGAAAATACTTCTGGCGCACCTTGTGGAAGGGAAAATGGCAAAAGGAGAAGAAATAGCGATAAAAAT
AGACCAGACTCTTACACAGGACTCTACTGGAACAATGGCTTATCTTCAGCTGGAGGCTTTGGGAATAGATAGAGTAAAGA
CAGAGCTTTCTGTCTCTTACGTTGACCACAATACTTTGCAGCAGGGACCGGAAAATGCTGACGACCACAAATACCTACAG
ACAGTCGCTGCAAGGTACGGAATATACTTTTCAAGGCCGGGAAATGGAATATGTCACCAGGTCCACCTTGAAAGATTTGG
AGTGCCAGGGAAAACCCTTTTGGGTTCAGACAGCCATACGCCCACTGCTGGTGGGTTGGGGATGTTGGCGATAGGAGCAG
GAGGACTCGATGTGGCTTTGGCAATGGCAGGAGAGCCTTATAGAATTATAATGCCTGCTATTGTAAATGTGAGGCTTAAA
GGTGAATTGAATCCATGGGTTTCGGCGAAAGATGTGATACTAGAGCTTTTAAGAAGACTTACTGTAAAAGGAGGCATAGG
TAAAATCTTCGAATATTCGGGGGATGGAGTAAAGACTCTGTCAGTGCCAGAAAGGACTACAATAGCGAATATGGGTGCGG
AATTGGGGGCGACTACCTCCATTTTTCCTTCTGATGAAAGGACTTACGAATTTTTAAAAGCTCAGGGTAGAGAAGATGTA
TTTGTGCCTTTGAGTGCGGATGAAGATGCGGAATATGATGAAGTCATAGAGATAGACCTTTCTGAATTGGAGCCCATGGT
GGCACTTCCTCACAGCCCGGACAATGTGGTTAAAGTGAAAGATGCTGGAAGGCCCAGGGTAGATCAGGTGGCGATAGGGT
CCTGCACCAATTCTTCTTATATGGATTTGATGAAAGTCGCTTCTATTTTAAAAGGGAAAACTATACCGGAGCATGTGAGC
CTTGTAATTGCACCAGGTTCTAGGCAGGTTTTGACTATGCTTGCGAAAAACGGTGCTTTAGCAGACCTTATAGCGGCTGG
AGCGAGAATACTTGAAAGTGCTTGTGGGCCCTGCATAGGAATGGGGCAAGCTCCTGCTACAGGCGCCGTTTCTGTGAGAA
CTTTTAATAGAAATTTCTATGGGAGAAGTGGGACAAAATCTGCATATGTGTATTTGGTAAGTCCGGAAGTAGCGGCGGCA
GCCGCGCTTACAGGGTATCTTATTGACCCTAGAGAACTGGGAGAACCACCTCAAGTGCCACTTGTTGAGAGATTTGAAGT
AAATGACAATATGATTGTAAAGCCGCCAGAGGATGGGAAAAACGTAGAAGTGATAAAGGGGCCAAATATAAAGCCATTTC
CATTGAATACGCCCCTTTCCGATATAGAAAAGAAGGTGTTGATAAAGGTAGGAGATGACATTACAACAGACCACATAATG
CCTTCAAATGCTAGACTTCTTCCTTTGAGGTCTAATATACCAGAGCTTTCGAAACATTGCTTTGAAATAATAGACGAAAA
TTTCTCAAAAAGAGCGGTTGAATGGGGTGGAGGGATAATTGTAGGAGGACATAACTATGGTCAGGGTTCAAGCCGCGAAC
ATGCAGCTTTGGTGCCTCTTTACTTGGGGGTTAAGGCGGTTATAGCTAAATCTTTTGCAAGAATACACAAGGCAAACCTC
ATAAACAGCGGAATTCTTCCTCTTACTTTTGTAAAAGAAGAGGACTATGAAGATATTGATATGGGAGATGTGCTAAAGAT
AGAGAATACAGTGGAGCAGGTGAGAAGTGGAAAAAACATTATTGTAAAGAATTTGACTAAAGGAAAAAGCTTTGAGGTAA
GACTGGAAGTTTCTGATAGAAATCGGGAAATCCTCATTGCAGGTGGAATGATAAATTATGTAAAAAGTAAAAATAAAACA
AATTGA

Upstream 100 bases:

>100_bases
AATTAAAGAGGCCTTTATTTGATAAGGAGCTAATGTACCTTTATGAAGATGTGATTGTGAAAGGAAAAGCTAAAGCAATC
TAAAAATAGGAGTGAAGAGG

Downstream 100 bases:

>100_bases
GGCACCTCACCAAAGAGGTGCCTATTTTTAAATCGGATGAGAGGAACCGTCCCCTCTGTCTGATATTTTTCTTGCGAAGA
TTAAATATCCTGTGTGCCCT

Product: aconitate hydratase

Products: NA

Alternate protein names: Alpha-IPM isomerase 2; IPMI 2; Isopropylmalate isomerase 2 [H]

Number of amino acids: Translated: 641; Mature: 641

Protein sequence:

>641_residues
MNLTQKILLAHLVEGKMAKGEEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVSYVDHNTLQQGPENADDHKYLQ
TVAARYGIYFSRPGNGICHQVHLERFGVPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPAIVNVRLK
GELNPWVSAKDVILELLRRLTVKGGIGKIFEYSGDGVKTLSVPERTTIANMGAELGATTSIFPSDERTYEFLKAQGREDV
FVPLSADEDAEYDEVIEIDLSELEPMVALPHSPDNVVKVKDAGRPRVDQVAIGSCTNSSYMDLMKVASILKGKTIPEHVS
LVIAPGSRQVLTMLAKNGALADLIAAGARILESACGPCIGMGQAPATGAVSVRTFNRNFYGRSGTKSAYVYLVSPEVAAA
AALTGYLIDPRELGEPPQVPLVERFEVNDNMIVKPPEDGKNVEVIKGPNIKPFPLNTPLSDIEKKVLIKVGDDITTDHIM
PSNARLLPLRSNIPELSKHCFEIIDENFSKRAVEWGGGIIVGGHNYGQGSSREHAALVPLYLGVKAVIAKSFARIHKANL
INSGILPLTFVKEEDYEDIDMGDVLKIENTVEQVRSGKNIIVKNLTKGKSFEVRLEVSDRNREILIAGGMINYVKSKNKT
N

Sequences:

>Translated_641_residues
MNLTQKILLAHLVEGKMAKGEEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVSYVDHNTLQQGPENADDHKYLQ
TVAARYGIYFSRPGNGICHQVHLERFGVPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPAIVNVRLK
GELNPWVSAKDVILELLRRLTVKGGIGKIFEYSGDGVKTLSVPERTTIANMGAELGATTSIFPSDERTYEFLKAQGREDV
FVPLSADEDAEYDEVIEIDLSELEPMVALPHSPDNVVKVKDAGRPRVDQVAIGSCTNSSYMDLMKVASILKGKTIPEHVS
LVIAPGSRQVLTMLAKNGALADLIAAGARILESACGPCIGMGQAPATGAVSVRTFNRNFYGRSGTKSAYVYLVSPEVAAA
AALTGYLIDPRELGEPPQVPLVERFEVNDNMIVKPPEDGKNVEVIKGPNIKPFPLNTPLSDIEKKVLIKVGDDITTDHIM
PSNARLLPLRSNIPELSKHCFEIIDENFSKRAVEWGGGIIVGGHNYGQGSSREHAALVPLYLGVKAVIAKSFARIHKANL
INSGILPLTFVKEEDYEDIDMGDVLKIENTVEQVRSGKNIIVKNLTKGKSFEVRLEVSDRNREILIAGGMINYVKSKNKT
N
>Mature_641_residues
MNLTQKILLAHLVEGKMAKGEEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVSYVDHNTLQQGPENADDHKYLQ
TVAARYGIYFSRPGNGICHQVHLERFGVPGKTLLGSDSHTPTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPAIVNVRLK
GELNPWVSAKDVILELLRRLTVKGGIGKIFEYSGDGVKTLSVPERTTIANMGAELGATTSIFPSDERTYEFLKAQGREDV
FVPLSADEDAEYDEVIEIDLSELEPMVALPHSPDNVVKVKDAGRPRVDQVAIGSCTNSSYMDLMKVASILKGKTIPEHVS
LVIAPGSRQVLTMLAKNGALADLIAAGARILESACGPCIGMGQAPATGAVSVRTFNRNFYGRSGTKSAYVYLVSPEVAAA
AALTGYLIDPRELGEPPQVPLVERFEVNDNMIVKPPEDGKNVEVIKGPNIKPFPLNTPLSDIEKKVLIKVGDDITTDHIM
PSNARLLPLRSNIPELSKHCFEIIDENFSKRAVEWGGGIIVGGHNYGQGSSREHAALVPLYLGVKAVIAKSFARIHKANL
INSGILPLTFVKEEDYEDIDMGDVLKIENTVEQVRSGKNIIVKNLTKGKSFEVRLEVSDRNREILIAGGMINYVKSKNKT
N

Specific function: Catalyzes the isomerization between 2-isopropylmalate and 3-isopropylmalate, via the formation of 2-isopropylmaleate [H]

COG id: COG1048

COG function: function code C; Aconitase A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the aconitase/IPM isomerase family. LeuC type 2 subfamily [H]

Homologues:

Organism=Homo sapiens, GI4501867, Length=718, Percent_Identity=33.2869080779944, Blast_Score=327, Evalue=2e-89,
Organism=Homo sapiens, GI8659555, Length=716, Percent_Identity=27.2346368715084, Blast_Score=203, Evalue=4e-52,
Organism=Homo sapiens, GI41352693, Length=394, Percent_Identity=30.4568527918782, Blast_Score=143, Evalue=5e-34,
Organism=Escherichia coli, GI87081781, Length=725, Percent_Identity=28.9655172413793, Blast_Score=240, Evalue=2e-64,
Organism=Escherichia coli, GI1786259, Length=465, Percent_Identity=28.6021505376344, Blast_Score=157, Evalue=2e-39,
Organism=Escherichia coli, GI1787531, Length=605, Percent_Identity=26.7768595041322, Blast_Score=141, Evalue=2e-34,
Organism=Escherichia coli, GI2367097, Length=428, Percent_Identity=30.1401869158879, Blast_Score=96, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI25149337, Length=724, Percent_Identity=34.9447513812155, Blast_Score=333, Evalue=2e-91,
Organism=Caenorhabditis elegans, GI32564738, Length=688, Percent_Identity=35.4651162790698, Blast_Score=323, Evalue=1e-88,
Organism=Caenorhabditis elegans, GI25149342, Length=574, Percent_Identity=35.0174216027875, Blast_Score=270, Evalue=2e-72,
Organism=Caenorhabditis elegans, GI17568399, Length=792, Percent_Identity=26.010101010101, Blast_Score=187, Evalue=2e-47,
Organism=Saccharomyces cerevisiae, GI6322261, Length=733, Percent_Identity=34.924965893588, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6323335, Length=729, Percent_Identity=35.6652949245542, Blast_Score=365, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6320440, Length=705, Percent_Identity=27.9432624113475, Blast_Score=222, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6321429, Length=663, Percent_Identity=27.7526395173454, Blast_Score=173, Evalue=6e-44,
Organism=Drosophila melanogaster, GI281365315, Length=719, Percent_Identity=34.6314325452017, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI17864292, Length=719, Percent_Identity=34.6314325452017, Blast_Score=335, Evalue=7e-92,
Organism=Drosophila melanogaster, GI161076999, Length=683, Percent_Identity=35.4319180087848, Blast_Score=327, Evalue=1e-89,
Organism=Drosophila melanogaster, GI28571643, Length=726, Percent_Identity=33.3333333333333, Blast_Score=326, Evalue=3e-89,
Organism=Drosophila melanogaster, GI24645686, Length=729, Percent_Identity=27.0233196159122, Blast_Score=170, Evalue=2e-42,
Organism=Drosophila melanogaster, GI17137564, Length=730, Percent_Identity=26.027397260274, Blast_Score=165, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015931
- InterPro:   IPR015937
- InterPro:   IPR001030
- InterPro:   IPR015932
- InterPro:   IPR018136
- InterPro:   IPR011826
- InterPro:   IPR015936
- InterPro:   IPR006251 [H]

Pfam domain/function: PF00330 Aconitase [H]

EC number: =4.2.1.33 [H]

Molecular weight: Translated: 69439; Mature: 69439

Theoretical pI: Translated: 6.22; Mature: 6.22

Prosite motif: PS00450 ACONITASE_1 ; PS01244 ACONITASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTQKILLAHLVEGKMAKGEEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVSY
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCHHHHEEEEEE
VDHNTLQQGPENADDHKYLQTVAARYGIYFSRPGNGICHQVHLERFGVPGKTLLGSDSHT
ECCHHHHCCCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEEEHHHCCCCCCEEECCCCCC
PTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPAIVNVRLKGELNPWVSAKDVILELLRRL
CCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
TVKGGIGKIFEYSGDGVKTLSVPERTTIANMGAELGATTSIFPSDERTYEFLKAQGREDV
HCCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHCCCCEEECCCCHHHHHHHHHCCCCCE
FVPLSADEDAEYDEVIEIDLSELEPMVALPHSPDNVVKVKDAGRPRVDQVAIGSCTNSSY
EEEECCCCCCCCCCEEEEEHHHCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCHH
MDLMKVASILKGKTIPEHVSLVIAPGSRQVLTMLAKNGALADLIAAGARILESACGPCIG
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
MGQAPATGAVSVRTFNRNFYGRSGTKSAYVYLVSPEVAAAAALTGYLIDPRELGEPPQVP
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHCEEECHHHCCCCCCCC
LVERFEVNDNMIVKPPEDGKNVEVIKGPNIKPFPLNTPLSDIEKKVLIKVGDDITTDHIM
CEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCC
PSNARLLPLRSNIPELSKHCFEIIDENFSKRAVEWGGGIIVGGHNYGQGSSREHAALVPL
CCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHEECCCEEEECCCCCCCCCCCCCEEEEHH
YLGVKAVIAKSFARIHKANLINSGILPLTFVKEEDYEDIDMGDVLKIENTVEQVRSGKNI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEE
IVKNLTKGKSFEVRLEVSDRNREILIAGGMINYVKSKNKTN
EEEECCCCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCCC
>Mature Secondary Structure
MNLTQKILLAHLVEGKMAKGEEIAIKIDQTLTQDSTGTMAYLQLEALGIDRVKTELSVSY
CCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCCCCEEEEEEEECCCCHHHHEEEEEE
VDHNTLQQGPENADDHKYLQTVAARYGIYFSRPGNGICHQVHLERFGVPGKTLLGSDSHT
ECCHHHHCCCCCCCHHHHHHHHHHHHCEEEECCCCCCEEEEEHHHCCCCCCEEECCCCCC
PTAGGLGMLAIGAGGLDVALAMAGEPYRIIMPAIVNVRLKGELNPWVSAKDVILELLRRL
CCCCCCEEEEECCCCCEEEEEECCCCEEEEEEEEEEEEEECCCCCCCCHHHHHHHHHHHH
TVKGGIGKIFEYSGDGVKTLSVPERTTIANMGAELGATTSIFPSDERTYEFLKAQGREDV
HCCCCCCEEEEECCCCEEEECCCCCHHHHHCCHHCCCCEEECCCCHHHHHHHHHCCCCCE
FVPLSADEDAEYDEVIEIDLSELEPMVALPHSPDNVVKVKDAGRPRVDQVAIGSCTNSSY
EEEECCCCCCCCCCEEEEEHHHCCCEEECCCCCCCEEEEECCCCCCCCEEEEECCCCCHH
MDLMKVASILKGKTIPEHVSLVIAPGSRQVLTMLAKNGALADLIAAGARILESACGPCIG
HHHHHHHHHHCCCCCCCCEEEEECCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCC
MGQAPATGAVSVRTFNRNFYGRSGTKSAYVYLVSPEVAAAAALTGYLIDPRELGEPPQVP
CCCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCHHHHHHHHCEEECHHHCCCCCCCC
LVERFEVNDNMIVKPPEDGKNVEVIKGPNIKPFPLNTPLSDIEKKVLIKVGDDITTDHIM
CEEEEECCCCEEEECCCCCCCEEEEECCCCCCCCCCCCHHHHCCEEEEEECCCCCCCCCC
PSNARLLPLRSNIPELSKHCFEIIDENFSKRAVEWGGGIIVGGHNYGQGSSREHAALVPL
CCCCEEEEECCCCHHHHHHHHHHHHCCHHHHHEECCCEEEECCCCCCCCCCCCCEEEEHH
YLGVKAVIAKSFARIHKANLINSGILPLTFVKEEDYEDIDMGDVLKIENTVEQVRSGKNI
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCEEEHHHHHHHHHCCCEE
IVKNLTKGKSFEVRLEVSDRNREILIAGGMINYVKSKNKTN
EEEECCCCCCEEEEEEECCCCCEEEEECCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]