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The map label for this gene is Cof [H]

Identifier: 20806983

GI number: 20806983

Start: 489747

End: 490571

Strand: Direct

Name: Cof [H]

Synonym: TTE0477

Alternate gene names: 20806983

Gene position: 489747-490571 (Clockwise)

Preceding gene: 20806979

Following gene: 20806984

Centisome position: 18.21

GC content: 39.03

Gene sequence:

>825_bases
ATGTATAAACTTCTTGTCAGTGATGCAGATGGGAGCTTGTTAAATAGCAATTCGGCGATTTCTGATCGAACTAAAGAGGC
TGTGAGAGAGGTGATATCGAGGGGAGTCATTTTTACAATAGCGACAGGCCGGATGTTCTCCTCTATCTTGCCCTATGCTG
AAGAGTTGAAAATAAATGCTCCTGTTATTTCCTATAACGGCGCTCTTATAAAAGACATATACACCAAAAAAGTGTACTAT
TACAATCCCATTCCTTCCGAAGATGCCCTGTTTGCCATAAAGCTTCTGAAAGAAAATGGATACCATATAAATTTATACAT
TGATGATGAACTTTATGTGGAAGAAATAACTGATAGAGTAGAGTGGTACCTTTCTTTTAACAACAATGTGAAGGTAAATC
CTGTGGGAGACCTCATGGAGTTTTTGATGGACACAGGCAGTGTTACGGCTAAGATTTACGCTCTAAATGACTTAAAAAAT
CCCGTCTCCATAGATGCGAGAGTGTATGATGAAATATCCAAGAAGTTGACCATTTCATCTTCCGGCGGAGGTCATTTAGA
GATAAATGCTAAAGGTGTGAGCAAAGGAAATGCTCTTAAAACCCTTGCCAATATGTACAACATAAAAAGGGAACAGGTAG
TGGCGATTGGCGACAATTTGAATGATCTTTCTATGATAGAATATGCAGGGTTGGGGGTTGCAATGGGTAATGCCCCGGAT
ATCGTAAAGATTAAGGCTTCCTATACAACTTTATCAAATGATGAAGATGGCGTTGCTCACGTCATAGATAAATTCTTTTT
GAACAGAAAAACCATAGCTGTGTAG

Upstream 100 bases:

>100_bases
TTTCAGTGGTAACGTTTTATAACAAATTATGATATAGAATGTAAATTTACAAGCTGTTATAATATAGATGAGGGATAATA
AAAATAGGGGTTGAAGAGGG

Downstream 100 bases:

>100_bases
TGACAAAAAAATAAAACAGGGAATATGATATATTACCGCAGTGCGGTAATATATTTTTTTATTCCCAGGTGATAAAATAT
GGAAGGGACGGTTATAAAAC

Product: HAD superfamily hydrolase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 274; Mature: 274

Protein sequence:

>274_residues
MYKLLVSDADGSLLNSNSAISDRTKEAVREVISRGVIFTIATGRMFSSILPYAEELKINAPVISYNGALIKDIYTKKVYY
YNPIPSEDALFAIKLLKENGYHINLYIDDELYVEEITDRVEWYLSFNNNVKVNPVGDLMEFLMDTGSVTAKIYALNDLKN
PVSIDARVYDEISKKLTISSSGGGHLEINAKGVSKGNALKTLANMYNIKREQVVAIGDNLNDLSMIEYAGLGVAMGNAPD
IVKIKASYTTLSNDEDGVAHVIDKFFLNRKTIAV

Sequences:

>Translated_274_residues
MYKLLVSDADGSLLNSNSAISDRTKEAVREVISRGVIFTIATGRMFSSILPYAEELKINAPVISYNGALIKDIYTKKVYY
YNPIPSEDALFAIKLLKENGYHINLYIDDELYVEEITDRVEWYLSFNNNVKVNPVGDLMEFLMDTGSVTAKIYALNDLKN
PVSIDARVYDEISKKLTISSSGGGHLEINAKGVSKGNALKTLANMYNIKREQVVAIGDNLNDLSMIEYAGLGVAMGNAPD
IVKIKASYTTLSNDEDGVAHVIDKFFLNRKTIAV
>Mature_274_residues
MYKLLVSDADGSLLNSNSAISDRTKEAVREVISRGVIFTIATGRMFSSILPYAEELKINAPVISYNGALIKDIYTKKVYY
YNPIPSEDALFAIKLLKENGYHINLYIDDELYVEEITDRVEWYLSFNNNVKVNPVGDLMEFLMDTGSVTAKIYALNDLKN
PVSIDARVYDEISKKLTISSSGGGHLEINAKGVSKGNALKTLANMYNIKREQVVAIGDNLNDLSMIEYAGLGVAMGNAPD
IVKIKASYTTLSNDEDGVAHVIDKFFLNRKTIAV

Specific function: Catalyzes the dephosphorylation of the artificial chromogenic substrate p-nitrophenyl phosphate (pNPP) and of the natural substrates erythrose 4-phosphate and mannose 1-phosphate [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=272, Percent_Identity=35.2941176470588, Blast_Score=137, Evalue=7e-34,
Organism=Escherichia coli, GI1786982, Length=266, Percent_Identity=31.203007518797, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI48994981, Length=276, Percent_Identity=27.536231884058, Blast_Score=92, Evalue=3e-20,
Organism=Escherichia coli, GI1787043, Length=265, Percent_Identity=28.6792452830189, Blast_Score=86, Evalue=2e-18,
Organism=Escherichia coli, GI87081790, Length=277, Percent_Identity=24.9097472924188, Blast_Score=74, Evalue=1e-14,
Organism=Escherichia coli, GI87081741, Length=239, Percent_Identity=23.4309623430962, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: NA

Molecular weight: Translated: 30328; Mature: 30328

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS00936 RIBOSOMAL_L35 ; PS01229 COF_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYKLLVSDADGSLLNSNSAISDRTKEAVREVISRGVIFTIATGRMFSSILPYAEELKINA
CEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCHHHEEECC
PVISYNGALIKDIYTKKVYYYNPIPSEDALFAIKLLKENGYHINLYIDDELYVEEITDRV
CEEEECCEEEEHHHHCEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHE
EWYLSFNNNVKVNPVGDLMEFLMDTGSVTAKIYALNDLKNPVSIDARVYDEISKKLTISS
EEEEEECCCEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHEEEEEC
SGGGHLEINAKGVSKGNALKTLANMYNIKREQVVAIGDNLNDLSMIEYAGLGVAMGNAPD
CCCCEEEEEECCCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHCCCEEEECCCCC
IVKIKASYTTLSNDEDGVAHVIDKFFLNRKTIAV
EEEEEEEEEECCCCCCHHHHHHHHHHHCCCEECC
>Mature Secondary Structure
MYKLLVSDADGSLLNSNSAISDRTKEAVREVISRGVIFTIATGRMFSSILPYAEELKINA
CEEEEEECCCCCEECCCCCCHHHHHHHHHHHHHCCEEEEEECCHHHHHHCCCHHHEEECC
PVISYNGALIKDIYTKKVYYYNPIPSEDALFAIKLLKENGYHINLYIDDELYVEEITDRV
CEEEECCEEEEHHHHCEEEEECCCCCCCCEEEEEEECCCCCEEEEEECCCHHHHHHHHHE
EWYLSFNNNVKVNPVGDLMEFLMDTGSVTAKIYALNDLKNPVSIDARVYDEISKKLTISS
EEEEEECCCEEECCHHHHHHHHHCCCCEEEEEEEECCCCCCEEEHHHHHHHHHHEEEEEC
SGGGHLEINAKGVSKGNALKTLANMYNIKREQVVAIGDNLNDLSMIEYAGLGVAMGNAPD
CCCCEEEEEECCCCCCHHHHHHHHHHCCCHHEEEEECCCCCCCHHHHHCCCEEEECCCCC
IVKIKASYTTLSNDEDGVAHVIDKFFLNRKTIAV
EEEEEEEEEECCCCCCHHHHHHHHHHHCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]