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The map label for this gene is aksA

Identifier: 20806978

GI number: 20806978

Start: 484493

End: 485647

Strand: Direct

Name: aksA

Synonym: TTE0472

Alternate gene names: 20806978

Gene position: 484493-485647 (Clockwise)

Preceding gene: 20806977

Following gene: 20806979

Centisome position: 18.01

GC content: 41.13

Gene sequence:

>1155_bases
ATGGCTTTCAAAAAAGACAAACCTGTTTACATTGTTGACACTACTTTAAGGGATGGTGAGCAAACTGCAGGTGTTGTATT
TGCAAATAACGAAAAAATCAGAATTGCGCAAATGTTAGATGAGATTGGCATAGACCAGCTGGAAGTTGGCATCCCCACAA
TGGGAGGAGATGAAAAGGAGACGGTAGCTAAAATTGCAAAATTAGGATTGAAGGCTAGTATAATGGCATGGAACAGAGCG
GTTGTAAAAGATGTTCAGGAATCTCTTGAGTGCGGTGTAGATGCAGTGGCCATTTCTATTTCTACATCAGACATTCACAT
AGAGCACAAGTTGAAAAAGACAAGGCAATGGGTATTGGACAGCATGACAGAAGCGGTGAGATTTGCTAAAAAAGAAGGCG
TTTATGTTTCTGTTAACGCGGAGGATGCCTCCAGGACTGATATGAATTTTTTGATTGAGTTTGCAAGGTGTGCAAAACAA
GCAGGGGCAGATAGATTGAGATTTTGCGACACTGTAGGTTTTTTGGATCCTTTCAAAACCTATGAGATGGTAAAAGCCAT
TAAAGATGCAGTAGATATTGAGATTGAAATGCATACCCATAATGATTTTGGTATGGCTACGGCAAACGCTCTTGCAGGAG
TTAAGGCTGGGGCTAAATTTGTGGGGGTTACAGTTAATGGACTAGGAGAGAGAGCTGGCAATGCGGCTTTAGAAGAGGTA
GTCATGGCGTTAAAGTACGTGTACAAAATGGATTTAGGTATTGACACCAGCCGCTTTAGAGAGATATCCGAGTATGTAGC
TTTAGCATCAGGTAGACCACTTCCTCCTTCTAAGGCTATTGTAGGTAAAAACGTGTTTGCCCATGAGTCAGGAATTCACG
TGGATGGAGCTCTTAAGAATCCGTATACTTATGAAGTGTTTGACCCTCAAGAAGTAGGACTTGAGAGGCAGATAGTGATA
GGAAAGCATTCTGGCACTGCGGCTTTAATAAACAAGTTTAAAGAGTACGGGAGAGTTTTAACTGAAGAGGAGGCTAATTT
GCTGCTCCCTCATGTTAGGAAGATGGCCATTCAATTAAAGAGGCCTTTATTTGATAAGGAGCTAATGTACCTTTATGAAG
ATGTGATTGTGAAAGGAAAAGCTAAAGCAATCTAA

Upstream 100 bases:

>100_bases
AGCTGCAATTTTTTATAATAAAAATTGCAAAATGCTATTGACTTTGATAAAGCTTCTGTGTAAAATAGGATGTAAAAGAG
TATTTGAAGGGGGATCATAT

Downstream 100 bases:

>100_bases
AAATAGGAGTGAAGAGGATGAATTTGACGCAGAAAATACTTCTGGCGCACCTTGTGGAAGGGAAAATGGCAAAAGGAGAA
GAAATAGCGATAAAAATAGA

Product: trans-homoaconitate synthase

Products: NA

Alternate protein names: Alpha-IPM synthase 2; Alpha-isopropylmalate synthase 2

Number of amino acids: Translated: 384; Mature: 383

Protein sequence:

>384_residues
MAFKKDKPVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVAKIAKLGLKASIMAWNRA
VVKDVQESLECGVDAVAISISTSDIHIEHKLKKTRQWVLDSMTEAVRFAKKEGVYVSVNAEDASRTDMNFLIEFARCAKQ
AGADRLRFCDTVGFLDPFKTYEMVKAIKDAVDIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVNGLGERAGNAALEEV
VMALKYVYKMDLGIDTSRFREISEYVALASGRPLPPSKAIVGKNVFAHESGIHVDGALKNPYTYEVFDPQEVGLERQIVI
GKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKMAIQLKRPLFDKELMYLYEDVIVKGKAKAI

Sequences:

>Translated_384_residues
MAFKKDKPVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVAKIAKLGLKASIMAWNRA
VVKDVQESLECGVDAVAISISTSDIHIEHKLKKTRQWVLDSMTEAVRFAKKEGVYVSVNAEDASRTDMNFLIEFARCAKQ
AGADRLRFCDTVGFLDPFKTYEMVKAIKDAVDIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVNGLGERAGNAALEEV
VMALKYVYKMDLGIDTSRFREISEYVALASGRPLPPSKAIVGKNVFAHESGIHVDGALKNPYTYEVFDPQEVGLERQIVI
GKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKMAIQLKRPLFDKELMYLYEDVIVKGKAKAI
>Mature_383_residues
AFKKDKPVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKETVAKIAKLGLKASIMAWNRAV
VKDVQESLECGVDAVAISISTSDIHIEHKLKKTRQWVLDSMTEAVRFAKKEGVYVSVNAEDASRTDMNFLIEFARCAKQA
GADRLRFCDTVGFLDPFKTYEMVKAIKDAVDIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVNGLGERAGNAALEEVV
MALKYVYKMDLGIDTSRFREISEYVALASGRPLPPSKAIVGKNVFAHESGIHVDGALKNPYTYEVFDPQEVGLERQIVIG
KHSGTAALINKFKEYGRVLTEEEANLLLPHVRKMAIQLKRPLFDKELMYLYEDVIVKGKAKAI

Specific function: Catalyzes the condensation of the acetyl group of acetyl-CoA with 3-methyl-2-oxobutanoate (2-oxoisovalerate) to form 3-carboxy-3-hydroxy-4-methylpentanoate (2-isopropylmalate)

COG id: COG0119

COG function: function code E; Isopropylmalate/homocitrate/citramalate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alpha-IPM synthase/homocitrate synthase family. LeuA type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1786261, Length=381, Percent_Identity=37.2703412073491, Blast_Score=207, Evalue=9e-55,
Organism=Saccharomyces cerevisiae, GI6320071, Length=366, Percent_Identity=32.5136612021858, Blast_Score=201, Evalue=2e-52,
Organism=Saccharomyces cerevisiae, GI6320019, Length=354, Percent_Identity=32.4858757062147, Blast_Score=200, Evalue=4e-52,
Organism=Saccharomyces cerevisiae, GI6324682, Length=355, Percent_Identity=21.9718309859155, Blast_Score=63, Evalue=8e-11,
Organism=Drosophila melanogaster, GI24582381, Length=285, Percent_Identity=25.2631578947368, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): LEU12_THETN (Q8RCF9)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622149.1
- ProteinModelPortal:   Q8RCF9
- SMR:   Q8RCF9
- GeneID:   998063
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE0472
- NMPDR:   fig|273068.3.peg.750
- HOGENOM:   HBG701974
- OMA:   VEMHTHN
- ProtClustDB:   PRK11858
- BioCyc:   TTEN273068:TTE0472-MONOMER
- BRENDA:   2.3.3.13
- HAMAP:   MF_01025_B
- InterPro:   IPR002034
- InterPro:   IPR013785
- InterPro:   IPR013477
- InterPro:   IPR000891
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR02660

Pfam domain/function: PF00682 HMGL-like

EC number: =2.3.3.13

Molecular weight: Translated: 42405; Mature: 42273

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00815 AIPM_HOMOCIT_SYNTH_1; PS00816 AIPM_HOMOCIT_SYNTH_2; PS50991 PYR_CT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFKKDKPVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKE
CCCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHH
TVAKIAKLGLKASIMAWNRAVVKDVQESLECGVDAVAISISTSDIHIEHKLKKTRQWVLD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEHHHHHHHHHHHHHH
SMTEAVRFAKKEGVYVSVNAEDASRTDMNFLIEFARCAKQAGADRLRFCDTVGFLDPFKT
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
YEMVKAIKDAVDIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVNGLGERAGNAALEEV
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
VMALKYVYKMDLGIDTSRFREISEYVALASGRPLPPSKAIVGKNVFAHESGIHVDGALKN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCEECCCCCC
PYTYEVFDPQEVGLERQIVIGKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKMAIQLK
CEEEEEECHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RPLFDKELMYLYEDVIVKGKAKAI
CCCCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
AFKKDKPVYIVDTTLRDGEQTAGVVFANNEKIRIAQMLDEIGIDQLEVGIPTMGGDEKE
CCCCCCCEEEEECCCCCCCCCCEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCHHH
TVAKIAKLGLKASIMAWNRAVVKDVQESLECGVDAVAISISTSDIHIEHKLKKTRQWVLD
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCEEHHHHHHHHHHHHHH
SMTEAVRFAKKEGVYVSVNAEDASRTDMNFLIEFARCAKQAGADRLRFCDTVGFLDPFKT
HHHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHH
YEMVKAIKDAVDIEIEMHTHNDFGMATANALAGVKAGAKFVGVTVNGLGERAGNAALEEV
HHHHHHHHHHHEEEEEEEECCCCCCHHHHHHHHHHCCCEEEEEEEECCCCCCCHHHHHHH
VMALKYVYKMDLGIDTSRFREISEYVALASGRPLPPSKAIVGKNVFAHESGIHVDGALKN
HHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCCHHHCCCEEECCCCCEECCCCCC
PYTYEVFDPQEVGLERQIVIGKHSGTAALINKFKEYGRVLTEEEANLLLPHVRKMAIQLK
CEEEEEECHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
RPLFDKELMYLYEDVIVKGKAKAI
CCCCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336