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The map label for this gene is PtsN2 [H]

Identifier: 20806858

GI number: 20806858

Start: 362668

End: 363108

Strand: Direct

Name: PtsN2 [H]

Synonym: TTE0341

Alternate gene names: 20806858

Gene position: 362668-363108 (Clockwise)

Preceding gene: 20806857

Following gene: 20806859

Centisome position: 13.48

GC content: 38.1

Gene sequence:

>441_bases
ATGGACAGAGAAATACTCAATGAAAAGAACATACTTTTAAACCTTCCTTCAGAGTCTAAAATAGAGGCTATTGAAAGGGT
AGGGAATCTCCTTGTTGAAAACGGCTATGTTGAAAAAGAGTATATCGAAGGTATGAAGAAAAGAGAAGAAGACATAACGA
CTTATATAGGAAATGGGGTAGCTATACCTCATGGGGTTTCTGAATATGTCAAATACATCAAAAAATCGGGGATAGTCATA
GCTCAGTATCCCGATGGGGTGGATTTTGGAGATGGCAACAGGGCCTATATTGTGATAGGAATTGCAGGTAAAGGAGATGA
GCATCTTAACATTCTTTCAAAGATAGCTTTGACATGCCAGTACGAAGAGAATGTAGAAAAGCTCAAAAAAGCAAAGACTC
CACAGGAGATAATAGAAATCCTGGAGAAAGGTGATGAGTAA

Upstream 100 bases:

>100_bases
TTGAGGACAAAAATTTTAGTGAGATTTTAAGGCTTGGGGATGTAAAAGATGTAAGAAATGCTTTGGTGAAAATATTAAAT
GAGGCATAGGAGGCGAAGTC

Downstream 100 bases:

>100_bases
AATGCTGAAGGCGCTTCACTTTGGAGCAGGCAATATAGGGAGAGGATTTATTGGATATTTACTTAATAAATCCGGATATG
AAGTTACCTTTGTAGATATT

Product: phosphotransferase system mannitol/fructose-specific IIA domain-containing protein

Products: protein histidine; sugar phosphate

Alternate protein names: EIIA-Mtl; EIII-Mtl; PTS system mannitol-specific EIIA component [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MDREILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGVAIPHGVSEYVKYIKKSGIVI
AQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQYEENVEKLKKAKTPQEIIEILEKGDE

Sequences:

>Translated_146_residues
MDREILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGVAIPHGVSEYVKYIKKSGIVI
AQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQYEENVEKLKKAKTPQEIIEILEKGDE
>Mature_146_residues
MDREILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGVAIPHGVSEYVKYIKKSGIVI
AQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQYEENVEKLKKAKTPQEIIEILEKGDE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=122, Percent_Identity=40.9836065573771, Blast_Score=110, Evalue=3e-26,
Organism=Escherichia coli, GI1790027, Length=138, Percent_Identity=37.6811594202899, Blast_Score=103, Evalue=3e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 16368; Mature: 16368

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDREILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGV
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AIPHGVSEYVKYIKKSGIVIAQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQ
CCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
YEENVEKLKKAKTPQEIIEILEKGDE
CHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MDREILNEKNILLNLPSESKIEAIERVGNLLVENGYVEKEYIEGMKKREEDITTYIGNGV
CCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCC
AIPHGVSEYVKYIKKSGIVIAQYPDGVDFGDGNRAYIVIGIAGKGDEHLNILSKIALTCQ
CCCCCHHHHHHHHHHCCEEEEECCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHCC
YEENVEKLKKAKTPQEIIEILEKGDE
CHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8824601 [H]