BacMap

Definition	Methanopyrus kandleri AV19, complete genome.
Accession	NC_003551
Length	1,694,969

Click here to switch to the map view.

The map label for this gene is HslV_2

Identifier: 20094664

GI number: 20094664

Start: 1213888

End: 1214520

Strand: Reverse

Name: HslV_2

Synonym: MK1228

Alternate gene names: 20094664

Gene position: 1214520-1213888 (Counterclockwise)

Preceding gene: 20094665

Following gene: 20094663

Centisome position: 71.65

GC content: 63.98

Gene sequence:

>633_bases
ATGAAGGAGCTGGACCAGCTCACTAAGGGAACCACGACCGTCGGCATACTGGCCGATAAAGGGGTCGTGTTGGCCGCCGA
CCGGCGAGCCGTGATGGGCAACCTGATCGCGGGCAAGCAGGTGAAGAAAATCTTCCGCATCCACGACTACATCGGCGTGA
CCACGGCCGGCTCGGTGGCCGACGCCCAGAAGATCGTCGACCTCATGCGGGCGGAGGCCAGACTGTACGAGCTGAGGCAC
AACCGGATGATCAGCGCCAGGGCGCTGGCCAACATGATCTCGCACGTGCTGCACTCGAGCCTCAAGGCGTTCCGCCCGTA
CCTCGTGCAACTCATCGTGGGCGGGTTCAACGACGACGATCCGGCGCTGTACAACCTGGACCCCAGCGGATCGATCATCG
AGGAGGACTACACGGCCACGGGTTCTGGCTCTCCGACGGCCTACGGTGTGCTGGAGGCCGAGTACGAGGAAGGGATGTCG
TTGGACGACGCGATCGAGGTGGCCGTCCGGGCCGTGAAATCCGCGCTCGAACGCGATACCGGTACCGGTGAGGGGGTGAC
CGTGGTAACCATCACCCGGGAGGAGGGGTACCGAGAGCTACCCGAGGAGGAGGTGGAGAAGCTCCTCTCGTGA

Upstream 100 bases:

>100_bases
GTGTCCGATCTGTGGTGAAACAGCCGAGTACACCGTCCGTATCGCGAAAACGTACTGAAACTGGAATCAGGGGTTGTCCG
GTTTGCAGTTGAGTGACGTG

Downstream 100 bases:

>100_bases
TCCCTCAGCCCCCTCCCCCGGACAATCGACCCCGGTGATCACCGGTGAAGCCAGGGGCGGATGCGTTGAGCGAGCGAAGA
GGCGGCGAAAAGACGTTGGA

Product: protease subunit of the proteasome

Products: NA

Alternate protein names: 20S proteasome beta subunit; Proteasome core protein PsmB

Number of amino acids: Translated: 210; Mature: 210

Protein sequence:

>210_residues
MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVADAQKIVDLMRAEARLYELRH
NRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDDPALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMS
LDDAIEVAVRAVKSALERDTGTGEGVTVVTITREEGYRELPEEEVEKLLS

Sequences:

>Translated_210_residues
MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVADAQKIVDLMRAEARLYELRH
NRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDDPALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMS
LDDAIEVAVRAVKSALERDTGTGEGVTVVTITREEGYRELPEEEVEKLLS
>Mature_210_residues
MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVADAQKIVDLMRAEARLYELRH
NRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDDPALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMS
LDDAIEVAVRAVKSALERDTGTGEGVTVVTITREEGYRELPEEEVEKLLS

Specific function: Component of the proteasome core, a large protease complex with broad specificity involved in protein degradation

COG id: COG0638

COG function: function code O; 20S proteasome, alpha and beta subunits

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase T1B family

Homologues:

Organism=Homo sapiens, GI73747875, Length=206, Percent_Identity=30.5825242718447, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI4758970, Length=206, Percent_Identity=30.5825242718447, Blast_Score=104, Evalue=5e-23,
Organism=Homo sapiens, GI4506201, Length=207, Percent_Identity=32.8502415458937, Blast_Score=101, Evalue=5e-22,
Organism=Homo sapiens, GI23110942, Length=210, Percent_Identity=26.1904761904762, Blast_Score=95, Evalue=5e-20,
Organism=Homo sapiens, GI156713442, Length=205, Percent_Identity=29.7560975609756, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4506185, Length=205, Percent_Identity=29.7560975609756, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI4506191, Length=185, Percent_Identity=31.8918918918919, Blast_Score=87, Evalue=2e-17,
Organism=Homo sapiens, GI22538465, Length=187, Percent_Identity=28.3422459893048, Blast_Score=86, Evalue=3e-17,
Organism=Homo sapiens, GI4506181, Length=203, Percent_Identity=30.5418719211823, Blast_Score=85, Evalue=5e-17,
Organism=Homo sapiens, GI23110925, Length=195, Percent_Identity=27.1794871794872, Blast_Score=84, Evalue=8e-17,
Organism=Homo sapiens, GI4506203, Length=187, Percent_Identity=28.3422459893048, Blast_Score=82, Evalue=3e-16,
Organism=Homo sapiens, GI153791216, Length=211, Percent_Identity=30.8056872037915, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI4506193, Length=201, Percent_Identity=27.363184079602, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI4506205, Length=187, Percent_Identity=26.7379679144385, Blast_Score=72, Evalue=3e-13,
Organism=Homo sapiens, GI4506189, Length=209, Percent_Identity=26.3157894736842, Blast_Score=71, Evalue=6e-13,
Organism=Homo sapiens, GI68303563, Length=203, Percent_Identity=27.0935960591133, Blast_Score=70, Evalue=9e-13,
Organism=Homo sapiens, GI23110935, Length=185, Percent_Identity=27.5675675675676, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI4506179, Length=185, Percent_Identity=27.5675675675676, Blast_Score=69, Evalue=3e-12,
Organism=Homo sapiens, GI195539356, Length=157, Percent_Identity=30.5732484076433, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI156713444, Length=166, Percent_Identity=27.710843373494, Blast_Score=67, Evalue=1e-11,
Organism=Homo sapiens, GI68303561, Length=211, Percent_Identity=26.0663507109005, Blast_Score=64, Evalue=7e-11,
Organism=Caenorhabditis elegans, GI17508499, Length=211, Percent_Identity=33.175355450237, Blast_Score=103, Evalue=8e-23,
Organism=Caenorhabditis elegans, GI17535357, Length=188, Percent_Identity=30.8510638297872, Blast_Score=96, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17508495, Length=192, Percent_Identity=30.2083333333333, Blast_Score=91, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17508493, Length=188, Percent_Identity=28.7234042553192, Blast_Score=89, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17508491, Length=210, Percent_Identity=27.6190476190476, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17562790, Length=210, Percent_Identity=27.1428571428571, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25143215, Length=212, Percent_Identity=26.8867924528302, Blast_Score=75, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17541700, Length=198, Percent_Identity=25.2525252525253, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325360, Length=204, Percent_Identity=30.3921568627451, Blast_Score=107, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6324731, Length=188, Percent_Identity=30.3191489361702, Blast_Score=88, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6321574, Length=193, Percent_Identity=27.4611398963731, Blast_Score=84, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6322459, Length=188, Percent_Identity=26.063829787234, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320941, Length=191, Percent_Identity=27.7486910994764, Blast_Score=79, Evalue=6e-16,
Organism=Saccharomyces cerevisiae, GI6319430, Length=213, Percent_Identity=27.2300469483568, Blast_Score=78, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6321692, Length=216, Percent_Identity=25.462962962963, Blast_Score=74, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324938, Length=173, Percent_Identity=30.635838150289, Blast_Score=73, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6320849, Length=195, Percent_Identity=22.5641025641026, Blast_Score=70, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6323547, Length=209, Percent_Identity=26.3157894736842, Blast_Score=70, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6324535, Length=176, Percent_Identity=24.4318181818182, Blast_Score=64, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6323974, Length=212, Percent_Identity=23.1132075471698, Blast_Score=63, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24654391, Length=213, Percent_Identity=27.6995305164319, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24654389, Length=213, Percent_Identity=27.6995305164319, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24584895, Length=199, Percent_Identity=30.6532663316583, Blast_Score=98, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130281, Length=199, Percent_Identity=30.6532663316583, Blast_Score=97, Evalue=6e-21,
Organism=Drosophila melanogaster, GI21355573, Length=203, Percent_Identity=31.5270935960591, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24652553, Length=203, Percent_Identity=31.5270935960591, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24639979, Length=184, Percent_Identity=30.4347826086957, Blast_Score=89, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28573221, Length=207, Percent_Identity=27.0531400966184, Blast_Score=85, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17737927, Length=209, Percent_Identity=26.7942583732057, Blast_Score=85, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24664391, Length=181, Percent_Identity=27.6243093922652, Blast_Score=82, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19921232, Length=211, Percent_Identity=26.5402843601896, Blast_Score=79, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24653999, Length=189, Percent_Identity=28.042328042328, Blast_Score=78, Evalue=4e-15,
Organism=Drosophila melanogaster, GI21355629, Length=190, Percent_Identity=24.7368421052632, Blast_Score=74, Evalue=6e-14,
Organism=Drosophila melanogaster, GI17136420, Length=216, Percent_Identity=26.3888888888889, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI17737605, Length=199, Percent_Identity=28.643216080402, Blast_Score=73, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19922898, Length=172, Percent_Identity=27.3255813953488, Blast_Score=71, Evalue=6e-13,
Organism=Drosophila melanogaster, GI17933602, Length=169, Percent_Identity=26.0355029585799, Blast_Score=64, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PSB_METKA (Q8TW10)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614511.1
- HSSP:   Q9P996
- ProteinModelPortal:   Q8TW10
- SMR:   Q8TW10
- MEROPS:   T01.002
- GeneID:   1477823
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1228
- NMPDR:   fig|190192.1.peg.1224
- HOGENOM:   HBG499923
- OMA:   MAYGVLE
- BioCyc:   MKAN190192:MK1228-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_02113_A
- InterPro:   IPR019983
- InterPro:   IPR000243
- InterPro:   IPR016050
- InterPro:   IPR001353
- PRINTS:   PR00141
- TIGRFAMs:   TIGR03634

Pfam domain/function: PF00227 Proteasome

EC number: =3.4.25.1

Molecular weight: Translated: 22828; Mature: 22828

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS00854 PROTEASOME_B_1; PS51476 PROTEASOME_B_2

Important sites: ACT_SITE 11-11

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVA
CCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DAQKIVDLMRAEARLYELRHNRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMSLDDAIEVAVRAVKSALERDT
CEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCC
GTGEGVTVVTITREEGYRELPEEEVEKLLS
CCCCCEEEEEEECCCCCCCCCHHHHHHHCC
>Mature Secondary Structure
MKELDQLTKGTTTVGILADKGVVLAADRRAVMGNLIAGKQVKKIFRIHDYIGVTTAGSVA
CCHHHHHHCCCCEEEEEECCCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHH
DAQKIVDLMRAEARLYELRHNRMISARALANMISHVLHSSLKAFRPYLVQLIVGGFNDDD
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
PALYNLDPSGSIIEEDYTATGSGSPTAYGVLEAEYEEGMSLDDAIEVAVRAVKSALERDT
CEEEEECCCCCEECCCCCCCCCCCCCEEEEEEEHHHCCCCHHHHHHHHHHHHHHHHHHCC
GTGEGVTVVTITREEGYRELPEEEVEKLLS
CCCCCEEEEEEECCCCCCCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014