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The map label for this gene is ProS

Identifier: 20094665

GI number: 20094665

Start: 1214563

End: 1216020

Strand: Reverse

Name: ProS

Synonym: MK1229

Alternate gene names: 20094665

Gene position: 1216020-1214563 (Counterclockwise)

Preceding gene: 20094667

Following gene: 20094664

Centisome position: 71.74

GC content: 61.39

Gene sequence:

>1458_bases
CTGCATGCGTTTCTTGGGAACGCTCAACTTCCGTCGACCCCGAGGGGTGATCGATTGGAGTTCTCGGAGTGGTACGCGGA
GGTCTTGCGCAGCGCGGAGATAATGGACGTGAGGTACCCCGTTAAGGGTATGTACGTGTGGCTACCGTATGGGTTCGAGA
TCCGACAGCGGGTAGTCGAGAAGCTGAGGCGCAAGCTGAGGGAGACCGGGCACGAGGAAGTACTGTTCCCGACGCTGATT
CCGGAGACCCAGCTGAAGAAGGAATCCGAGCACATAGCAGGGTTCGAGGACGAGGTGTACTGGGTGACCCACGGGGGTCT
CAAGGAGCTCGACGAGAAGCTGGCGCTGCGGCCGACCAGCGAGACGGCGATCTACCCGATGTTCGCGCTGTGGATCAGGT
CACACGCCGACCTACCGCTCAAGATATTCCAGATCGTGAACACCTTCCGGTACGAGACGAAGCACACCCGACCGCTGATC
CGGATGCGGGAGATCACCACGTTCAAGGAAGCCCACACAGCCCACGCGACCGAGGAGGAGGCGGAGGAGCAAGTCAAGGA
GGCGGTGGAGATCTACTCGTCGTTCTTCGACGAGCTCGGGATCCCGTACATCGCGTCGGTGCGACCGGAGTGGGACAAGT
TCCCGGGCGCCGAGTACACGGTGGCCTTCGACACGCTGATGCCGGACGGTCGGACGCTGCAGATCGGTACCGTCCACATG
CTCGGCCAGAACTTCGCGAGAACGTTCGAGGTCACCTACGAGACGGAGGAGGGTGACCAGGAGTACGTGTACATGACCTG
CTACGGTATATCGGACCGAGTGGTGGCCTCGATGATAGCCATACACGGGGACGAGCGCGGCCTGGTGCTACCGCCGGACG
TGGCTCCGTACCAGGTTGTGATGGTCCCCATACTGAAGAAGGGAGTCCGGCGGAAGATCCTGGAACGTGCCGCGGAGGTC
GAGGAGATGCTGCGGGAGGAGGGAGTCCGGGTCAAGGTGGACGACCGCGACATGAGCCCGGGACGCAAGTTCCATTACTG
GGAGCTGAAGGGTGTCCCGCTGCGTATCGAGCTCGGAGCCCGGGAACTCGAGGAGGGTACCGCGGTAGTCTTCCGGAGGG
ACGAGCTCGAGCGCGAGACGTACGCGTTCGAAGAACTGCCCGACGTCGTCCCTGAGCTGCTGGAAGACATCGCGATGGAG
CTGCGGAAGCGAGCCCGCGAGAAGTTCGAAAAGGGTATATTCCGAACGGACTCCCCGGAGGAAGCACGAAGACTGGTTGG
TGAGGGGATCGTCGAGACCGGTTGGTGCGGTTCCGAGCGCTGCGGTGTACGTATGGAGGAGGAGTTTGGTGGAGACGTTC
TCGGTACGCCGTACCCCGAAGAAGACACCGAATTCGAAAGGTGTCCGATCTGTGGTGAAACAGCCGAGTACACCGTCCGT
ATCGCGAAAACGTACTGA

Upstream 100 bases:

>100_bases
CGGAGAACACCGCGACGACTCCGTCCGGTACTCCGACGCTACGTAGGAGCTTCGGAACTTCGGGGAGCACGTTCGAACCC
ACGACGACCTCTCTCGGGAG

Downstream 100 bases:

>100_bases
AACTGGAATCAGGGGTTGTCCGGTTTGCAGTTGAGTGACGTGATGAAGGAGCTGGACCAGCTCACTAAGGGAACCACGAC
CGTCGGCATACTGGCCGATA

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVEKLRRKLRETGHEEVLFPTLI
PETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTSETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLI
RMREITTFKEAHTAHATEEEAEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHM
LGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVVMVPILKKGVRRKILERAAEV
EEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGARELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAME
LRKRAREKFEKGIFRTDSPEEARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVR
IAKTY

Sequences:

>Translated_485_residues
MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVEKLRRKLRETGHEEVLFPTLI
PETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTSETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLI
RMREITTFKEAHTAHATEEEAEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHM
LGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVVMVPILKKGVRRKILERAAEV
EEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGARELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAME
LRKRAREKFEKGIFRTDSPEEARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVR
IAKTY
>Mature_485_residues
MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVEKLRRKLRETGHEEVLFPTLI
PETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTSETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLI
RMREITTFKEAHTAHATEEEAEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHM
LGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVVMVPILKKGVRRKILERAAEV
EEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGARELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAME
LRKRAREKFEKGIFRTDSPEEARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVR
IAKTY

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro)

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 3 subfamily

Homologues:

Organism=Homo sapiens, GI62241042, Length=433, Percent_Identity=35.796766743649, Blast_Score=297, Evalue=1e-80,
Organism=Escherichia coli, GI1786392, Length=147, Percent_Identity=36.0544217687075, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI71991342, Length=486, Percent_Identity=32.7160493827161, Blast_Score=288, Evalue=5e-78,
Organism=Caenorhabditis elegans, GI71991346, Length=356, Percent_Identity=37.9213483146067, Blast_Score=267, Evalue=8e-72,
Organism=Saccharomyces cerevisiae, GI6321808, Length=449, Percent_Identity=36.0801781737194, Blast_Score=297, Evalue=3e-81,
Organism=Drosophila melanogaster, GI24649468, Length=493, Percent_Identity=33.8742393509128, Blast_Score=276, Evalue=2e-74,
Organism=Drosophila melanogaster, GI24649466, Length=394, Percent_Identity=38.0710659898477, Blast_Score=274, Evalue=8e-74,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): SYP_METKA (Q8TW09)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614512.1
- HSSP:   O26708
- ProteinModelPortal:   Q8TW09
- SMR:   Q8TW09
- GeneID:   1477824
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1229
- NMPDR:   fig|190192.1.peg.1225
- HOGENOM:   HBG334108
- OMA:   PGWKFNE
- ProtClustDB:   PRK08661
- BioCyc:   MKAN190192:MK1229-MONOMER
- BRENDA:   6.1.1.15
- GO:   GO:0005737
- HAMAP:   MF_01571
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004499
- InterPro:   IPR017449
- InterPro:   IPR016061
- Gene3D:   G3DSA:3.40.50.800
- Gene3D:   G3DSA:3.30.110.30
- PANTHER:   PTHR11451:SF6
- PRINTS:   PR01046
- SMART:   SM00946
- TIGRFAMs:   TIGR00408

Pfam domain/function: PF03129 HGTP_anticodon; PF09180 ProRS-C_1; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd; SSF64586 Pro-tRNA_synth_II_C

EC number: =6.1.1.15

Molecular weight: Translated: 56219; Mature: 56219

Theoretical pI: Translated: 4.68; Mature: 4.68

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEECCCCCEEEEECCCHHHHHHHHH
KLRRKLRETGHEEVLFPTLIPETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTS
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCC
ETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLIRMREITTFKEAHTAHATEEE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHM
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCCEEEEEECCCCCCCEEEEEHHHH
LGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVV
HHHHHHEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCHHHH
MVPILKKGVRRKILERAAEVEEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCEEEEECCC
RELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAMELRKRAREKFEKGIFRTDSPE
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVR
HHHHHHHCCCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
IAKTY
EEECC
>Mature Secondary Structure
MHAFLGNAQLPSTPRGDRLEFSEWYAEVLRSAEIMDVRYPVKGMYVWLPYGFEIRQRVVE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCHHEEEECCCCCEEEEECCCHHHHHHHHH
KLRRKLRETGHEEVLFPTLIPETQLKKESEHIAGFEDEVYWVTHGGLKELDEKLALRPTS
HHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHCCCCCCEEEEECCCHHHHHHHHCCCCCC
ETAIYPMFALWIRSHADLPLKIFQIVNTFRYETKHTRPLIRMREITTFKEAHTAHATEEE
CCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHH
AEEQVKEAVEIYSSFFDELGIPYIASVRPEWDKFPGAEYTVAFDTLMPDGRTLQIGTVHM
HHHHHHHHHHHHHHHHHHCCCCEEECCCCCHHHCCCCCEEEEEECCCCCCCEEEEEHHHH
LGQNFARTFEVTYETEEGDQEYVYMTCYGISDRVVASMIAIHGDERGLVLPPDVAPYQVV
HHHHHHEEEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCCCEEECCCCCCHHHH
MVPILKKGVRRKILERAAEVEEMLREEGVRVKVDDRDMSPGRKFHYWELKGVPLRIELGA
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCEEEEEEECCCEEEEECCC
RELEEGTAVVFRRDELERETYAFEELPDVVPELLEDIAMELRKRAREKFEKGIFRTDSPE
HHHCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHH
EARRLVGEGIVETGWCGSERCGVRMEEEFGGDVLGTPYPEEDTEFERCPICGETAEYTVR
HHHHHHHCCCCCCCCCCCCCCCCEEHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEE
IAKTY
EEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014