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The map label for this gene is PurM

Identifier: 20094662

GI number: 20094662

Start: 1210813

End: 1211850

Strand: Reverse

Name: PurM

Synonym: MK1226

Alternate gene names: 20094662

Gene position: 1211850-1210813 (Counterclockwise)

Preceding gene: 20094663

Following gene: 20094648

Centisome position: 71.5

GC content: 66.86

Gene sequence:

>1038_bases
TTGTCCGAGATGACCTACAAGGACGCCGGAGTGGACATCGACAAGGAGGCGTTCGCCGTCCGGGCGATCCGGGACGTGCT
GGAGAAGTACAAGGTGGAGCCGGAGGGCTGCCGGGAGGTCGAGGGCATCGGCCACTACGCCGCCGTGCTCGAGGTGCACG
GGGAACTGCTGACGCTCAACGTGGACGGCGTGGGGTCCAAGGTCCTCGTCGCCCAGCTGGTCGGCCGGTACGACACCGTC
GGCATCGACGCGATAGCCATGAACGCGAACGACGCGGTGTGCCTCGGGGCGCGGCCGCTGGCCTTCCTCGACTACCTGGC
GATGGAGGATCCCGACCCCGACGTCTGCGCGGAGATCGCGGAGGGACTCGGCAAGGGAGCCCGAGAGGCGGGTGCCCCGA
TCGTCGGCGGCGAGCTGGCGACGCTCCCGGAGGTGATCCGCGGAAAGGAGGAAGGACGTGGGTTCGACCTCGTGGTCGCC
TGTCTGGGTCGGGTCGAGGGGGACCCGATCACGGGGGAGGACGTGGAACCCGGCGACGCGATCGTGGGACTGCGGAGCTC
CGGGATCCACAGCAACGGCCTGACGCTAGCTAGGAAGGTCCTCCTCTCGGAGTACGACGTGCACGACGAGCTCCCGCACG
GTAGGACCGTGGCCGAGGAGCTCCTCGAACCGACGCGCATCTACGTCCGGCCCGTGATGGAGGTACTGCGGGACTACGAG
GTGCGCGGAATCGCCCACATCACCGGGGGTGGAGTGGAGAACCTGAAGCGGCTCCGAGACGACGTGCGCTACGTGCTGGA
CGACCCGTTCGAGCCGCATCCCGTGTTCGAGATAATCAGGGAGCTGGGGAACGTGCCCGTCGAAGAGATGTACCGTACGT
TCAACATGGGAATGGGCATGGCGTTGATCGTCCCGGAGGAGGAGGCCGAAGACGTCGTCGACACGGTGTCGAAGCACGTG
GAGGCGAAGATCGTGGGACACGTGGAGGAGGGACGCGGGGTGGTCGTCCACATGGACGGGCACGAGGTGAAACTCTGA

Upstream 100 bases:

>100_bases
AAGTACTTCTCCAACCACCTGCGCCGCACGGTCAGGAAGGTCTTTTCCATGGCGCCCGAGAACCTGGAGTGCTTCCGTCT
CACTTAGGGGGTACGACGAG

Downstream 100 bases:

>100_bases
GTCAACAAAACCCGGATCACGCGTTCAGGACGACGATCCCCAGTTCTTCGGCCTTCTCGAGGATCTCCTGGCGCTTCCTC
CTACCCACTCGACTGGCGAT

Product: phosphoribosylaminoimidazole synthetase

Products: NA

Alternate protein names: AIR synthase; AIRS; Phosphoribosyl-aminoimidazole synthetase

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MSEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLNVDGVGSKVLVAQLVGRYDTV
GIDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIAEGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVA
CLGRVEGDPITGEDVEPGDAIVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYE
VRGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGMALIVPEEEAEDVVDTVSKHV
EAKIVGHVEEGRGVVVHMDGHEVKL

Sequences:

>Translated_345_residues
MSEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLNVDGVGSKVLVAQLVGRYDTV
GIDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIAEGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVA
CLGRVEGDPITGEDVEPGDAIVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYE
VRGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGMALIVPEEEAEDVVDTVSKHV
EAKIVGHVEEGRGVVVHMDGHEVKL
>Mature_344_residues
SEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLNVDGVGSKVLVAQLVGRYDTVG
IDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIAEGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVAC
LGRVEGDPITGEDVEPGDAIVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYEV
RGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGMALIVPEEEAEDVVDTVSKHVE
AKIVGHVEEGRGVVVHMDGHEVKL

Specific function: De novo purine biosynthesis; fifth step. [C]

COG id: COG0150

COG function: function code F; Phosphoribosylaminoimidazole (AIR) synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AIR synthase family

Homologues:

Organism=Homo sapiens, GI4503915, Length=341, Percent_Identity=35.4838709677419, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI209869995, Length=341, Percent_Identity=35.4838709677419, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI209869993, Length=341, Percent_Identity=35.4838709677419, Blast_Score=181, Evalue=1e-45,
Organism=Escherichia coli, GI1788845, Length=326, Percent_Identity=39.5705521472393, Blast_Score=203, Evalue=2e-53,
Organism=Caenorhabditis elegans, GI17567511, Length=349, Percent_Identity=33.810888252149, Blast_Score=165, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6321203, Length=330, Percent_Identity=36.0606060606061, Blast_Score=182, Evalue=7e-47,
Organism=Drosophila melanogaster, GI24582400, Length=282, Percent_Identity=38.2978723404255, Blast_Score=169, Evalue=3e-42,

Paralogues:

None

Copy number: 180 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): PUR5_METKA (Q8TW12)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614509.1
- ProteinModelPortal:   Q8TW12
- SMR:   Q8TW12
- GeneID:   1477821
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1226
- NMPDR:   fig|190192.1.peg.1222
- HOGENOM:   HBG531222
- OMA:   HYDLAGF
- ProtClustDB:   PRK05385
- BioCyc:   MKAN190192:MK1226-MONOMER
- BRENDA:   6.3.3.1
- GO:   GO:0005737
- HAMAP:   MF_00741_A
- InterPro:   IPR000728
- InterPro:   IPR010918
- InterPro:   IPR004733
- InterPro:   IPR016188
- TIGRFAMs:   TIGR00878

Pfam domain/function: PF00586 AIRS; PF02769 AIRS_C; SSF56042 AIR_synth_C; SSF55326 PurM_N-like

EC number: =6.3.3.1

Molecular weight: Translated: 37492; Mature: 37361

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLN
CCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHCCCCEEEEE
VDGVGSKVLVAQLVGRYDTVGIDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIA
ECCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHH
EGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVACLGRVEGDPITGEDVEPGDA
HHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCE
IVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYE
EEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VRGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGM
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCE
ALIVPEEEAEDVVDTVSKHVEAKIVGHVEEGRGVVVHMDGHEVKL
EEEECCHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCEECC
>Mature Secondary Structure 
SEMTYKDAGVDIDKEAFAVRAIRDVLEKYKVEPEGCREVEGIGHYAAVLEVHGELLTLN
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHCHHHHHHHHCCCCEEEEE
VDGVGSKVLVAQLVGRYDTVGIDAIAMNANDAVCLGARPLAFLDYLAMEDPDPDVCAEIA
ECCCCHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCHHHHHHHHHCCCCCHHHHHHHH
EGLGKGAREAGAPIVGGELATLPEVIRGKEEGRGFDLVVACLGRVEGDPITGEDVEPGDA
HHHCCCHHHHCCCCCCCHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCE
IVGLRSSGIHSNGLTLARKVLLSEYDVHDELPHGRTVAEELLEPTRIYVRPVMEVLRDYE
EEEECCCCCCCCCHHHHHHHHHHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
VRGIAHITGGGVENLKRLRDDVRYVLDDPFEPHPVFEIIRELGNVPVEEMYRTFNMGMGM
CCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCCHHHHHHHHHCCCCE
ALIVPEEEAEDVVDTVSKHVEAKIVGHVEEGRGVVVHMDGHEVKL
EEEECCHHHHHHHHHHHHHHHHHHEEEEECCCEEEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014