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The map label for this gene is PurD

Identifier: 20094507

GI number: 20094507

Start: 1038509

End: 1039858

Strand: Reverse

Name: PurD

Synonym: MK1071

Alternate gene names: 20094507

Gene position: 1039858-1038509 (Counterclockwise)

Preceding gene: 20094508

Following gene: 20094506

Centisome position: 61.35

GC content: 61.41

Gene sequence:

>1350_bases
ATGCTGAAAGTACTGCTCGTGGGTAGCGGCGCCCGAGAACACGCGATCGCAGAGGCGCTTTGCGGAGCTCCGGACGAGGA
CGTAGAGCTTTACGCGTTCATGGGGAACCGGAACCCGGGTATCATCCGACTGGCCGAGGACTACGTCGTAGGAGACCCGA
CCGACGTGGAAGCGGTGGCTCGAGCCGCCGCAGACTGGAACGTGGACTTCGCGGTGGTGGGGCCGGAAGACCCGCTGGCC
GAGGGAGTGGCGGACCGCCTGGAGGAGGAAGGCATCCCCACGTTCGGCCCGAAACGGGGCCCGGCTCGGATCGAGTGGGA
TAAGGGCTTCGCACGCGAGCTGATGGAGAAGTACGACATTCCGGGCCGCCCGGAGTTCGGGATATTCGAGGATCCCGACG
AGGCTTGCGACTTCATCGACGAGCTCGGTAAACCCGTAGCCGTCAAGCCGGCGGGTCTGACCGGCGGTAAGGGAGTGAAG
GTGGTCGGCGACCAGCTGAAGAATCTCGACGAAGCGAAGGAATACGTGAAGGAGATCTTCGAGGAGGACATCGGCGGGAT
CCCCAAAGTGATCATCGAGGAGAAGTGCGTGGGTGAGGAGTACACGATCCAGGCCTACACGGACGGGGAAAAGGTGATCC
CCACACCAGCCGTACAGGATCACCCACACGCGTACGAGGGTGATAAGGGCCCCATCACTGGTGGAATGGGCTCGTACTCG
TGTCCCGATGGTCTGTTACCGTTCATAACTAAAGAGGATTACGAACGTTCCGTCGAGATCCTAGAACGCACTGTCGAGGC
GATCAAGAAGGAAACCGGTGAGCCGTACCGAGGGGTTCTATACGGGCAGTTCATGCTGACCGCGGAAGGTCCCGTCGTCA
TCGAGTTCAACTGCCGGTACGGAGACCCCGAAGCCATGAACATCCTCCCGATCGCCGAAGGCGACATCGTAACGCTTCAC
GCGTCGATCGCCGAGGGGTCGATGGAGGGAGAGATCGAGTTCCTCGAAAAGGCCACCGTGTGCAAGTACGTCGTCCCCGA
AGGGTACCCGGAGTCGTCGGAAGGAGAGGGAGACGTCATCGAGGTGGACGAGGAGTGCATCCATAGGTATGACGCGGTCC
CGTACTACGCTTCCGTGAACCTGGATGAGGACGGAAAGATCCGGATGACCTCCTCCCGCGCCCTGGCGATCGTGGGTATC
GGCGACGAGCTCGAGCAGGCGGAAGAAGCCGCGGAGAGTGCGATCCGTGAGTGCGTGAGCGGGGAAAGGATTCGGCACCG
GTCAGACATCGGAAAGCATGAGACCGTTGAGAAGCGGGTGCGCCGTATGAAGCGAATCCGTGGGGAGTAG

Upstream 100 bases:

>100_bases
GTCGTGGTCGATCGACAGGAGGGGGCGGAAGAAGCGCTTAAAGAGCGGGGTGTGAGGCTCTCGAGCGTGCTGACGGCCGA
CGACCTGCGGGGGCTGCGGG

Downstream 100 bases:

>100_bases
TGAGGTTGAAGCGCCTCAGCACCAACCATCTCCTTTCCATCGCGGATTTAGATCGGGAAGATGTCGAAACGGTCCTGCGC
GTGGCCGAACGGTTCAAGGA

Product: phosphoribosylamine-glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase

Number of amino acids: Translated: 449; Mature: 449

Protein sequence:

>449_residues
MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVARAAADWNVDFAVVGPEDPLA
EGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDIPGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVK
VVGDQLKNLDEAKEYVKEIFEEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYS
CPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRYGDPEAMNILPIAEGDIVTLH
ASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVIEVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGI
GDELEQAEEAAESAIRECVSGERIRHRSDIGKHETVEKRVRRMKRIRGE

Sequences:

>Translated_449_residues
MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVARAAADWNVDFAVVGPEDPLA
EGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDIPGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVK
VVGDQLKNLDEAKEYVKEIFEEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYS
CPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRYGDPEAMNILPIAEGDIVTLH
ASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVIEVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGI
GDELEQAEEAAESAIRECVSGERIRHRSDIGKHETVEKRVRRMKRIRGE
>Mature_449_residues
MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVARAAADWNVDFAVVGPEDPLA
EGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDIPGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVK
VVGDQLKNLDEAKEYVKEIFEEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYS
CPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRYGDPEAMNILPIAEGDIVTLH
ASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVIEVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGI
GDELEQAEEAAESAIRECVSGERIRHRSDIGKHETVEKRVRRMKRIRGE

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

Organism=Homo sapiens, GI4503915, Length=435, Percent_Identity=34.7126436781609, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI209869995, Length=435, Percent_Identity=34.7126436781609, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI209869993, Length=435, Percent_Identity=34.7126436781609, Blast_Score=213, Evalue=3e-55,
Organism=Homo sapiens, GI28416899, Length=435, Percent_Identity=34.7126436781609, Blast_Score=213, Evalue=3e-55,
Organism=Escherichia coli, GI1790438, Length=438, Percent_Identity=31.5068493150685, Blast_Score=192, Evalue=3e-50,
Organism=Caenorhabditis elegans, GI17567511, Length=439, Percent_Identity=29.8405466970387, Blast_Score=158, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6321203, Length=450, Percent_Identity=27.7777777777778, Blast_Score=169, Evalue=7e-43,
Organism=Drosophila melanogaster, GI62471619, Length=442, Percent_Identity=34.841628959276, Blast_Score=198, Evalue=5e-51,
Organism=Drosophila melanogaster, GI24582400, Length=441, Percent_Identity=34.9206349206349, Blast_Score=193, Evalue=2e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PUR2_METKA (Q8TWG3)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614354.1
- ProteinModelPortal:   Q8TWG3
- SMR:   Q8TWG3
- GeneID:   1477172
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1071
- NMPDR:   fig|190192.1.peg.1067
- HOGENOM:   HBG404060
- OMA:   GVPIVIK
- BioCyc:   MKAN190192:MK1071-MONOMER
- BRENDA:   6.3.4.13
- HAMAP:   MF_00138
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020562
- InterPro:   IPR011054
- Gene3D:   G3DSA:3.30.1490.20
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.90.600.10
- Gene3D:   G3DSA:3.40.50.20
- TIGRFAMs:   TIGR00877

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N; SSF52440 PreATP-grasp-like; SSF51246 Rudmnt_hyb_motif

EC number: =6.3.4.13

Molecular weight: Translated: 49276; Mature: 49276

Theoretical pI: Translated: 4.20; Mature: 4.20

Prosite motif: PS50975 ATP_GRASP; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVA
CEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECCEECCCCCCHHHHH
RAAADWNVDFAVVGPEDPLAEGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDI
HHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCC
PGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVKVVGDQLKNLDEAKEYVKEIF
CCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
EEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYS
HHHHCCCCHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
CPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEEEECCC
GDPEAMNILPIAEGDIVTLHASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVI
CCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCEE
EVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGIGDELEQAEEAAESAIRECVS
ECCHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHC
GERIRHRSDIGKHETVEKRVRRMKRIRGE
CHHHHHHHCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKVLLVGSGAREHAIAEALCGAPDEDVELYAFMGNRNPGIIRLAEDYVVGDPTDVEAVA
CEEEEEECCCCHHHHHHHHHCCCCCCCCEEEEEECCCCCCEEEEECCEECCCCCCHHHHH
RAAADWNVDFAVVGPEDPLAEGVADRLEEEGIPTFGPKRGPARIEWDKGFARELMEKYDI
HHHCCCCCEEEEECCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEECCHHHHHHHHHCCC
PGRPEFGIFEDPDEACDFIDELGKPVAVKPAGLTGGKGVKVVGDQLKNLDEAKEYVKEIF
CCCCCCCCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCHHHHHHHHCCHHHHHHHHHHHH
EEDIGGIPKVIIEEKCVGEEYTIQAYTDGEKVIPTPAVQDHPHAYEGDKGPITGGMGSYS
HHHHCCCCHHHHHHHHCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCCCCCCCCC
CPDGLLPFITKEDYERSVEILERTVEAIKKETGEPYRGVLYGQFMLTAEGPVVIEFNCRY
CCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCEEEEEECCC
GDPEAMNILPIAEGDIVTLHASIAEGSMEGEIEFLEKATVCKYVVPEGYPESSEGEGDVI
CCCCCEEEEEECCCCEEEEEEECCCCCCCCHHHHHHHHHHHEEECCCCCCCCCCCCCCEE
EVDEECIHRYDAVPYYASVNLDEDGKIRMTSSRALAIVGIGDELEQAEEAAESAIRECVS
ECCHHHHHHHCCCCEEEEECCCCCCCEEEECCCEEEEEECCHHHHHHHHHHHHHHHHHHC
GERIRHRSDIGKHETVEKRVRRMKRIRGE
CHHHHHHHCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014