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The map label for this gene is ArgF

Identifier: 20094506

GI number: 20094506

Start: 1037571

End: 1038509

Strand: Reverse

Name: ArgF

Synonym: MK1070

Alternate gene names: 20094506

Gene position: 1038509-1037571 (Counterclockwise)

Preceding gene: 20094507

Following gene: 20094505

Centisome position: 61.27

GC content: 60.7

Gene sequence:

>939_bases
GTGAGGTTGAAGCGCCTCAGCACCAACCATCTCCTTTCCATCGCGGATTTAGATCGGGAAGATGTCGAAACGGTCCTGCG
CGTGGCCGAACGGTTCAAGGAACGCTACCTCGCGGGCGAGAGGGTAATACCGATACTGGAAGGCAAGACACTCGGCCTAA
TCTTCGAGAAACCCTCCACCCGCACCCGCGTCTCCTTCGAGGTGGCCATGCACCAGCTGGGCGGGCAGGCTTTCACGTAC
ACAAAACAGGAGTTGCAGCTGGGTCGAGGTGAGGCGATCAAAGACACGGCGGCCGTGCTGAGCAGGTATCTGGACGGAGT
CATGATCCGAGCGCGCCGCCACGAAGACATCGAGGAGTTCGCCCGGTACTCGGAGGTACCCGTCATCAACGGCCTGAGTG
ACCTCGAACACCCCTGTCAAGCTCTTACGGACGCCTTCACGATCCGGGAGAAGCTCGGTCGAGGTCCCCACACGGTCGCC
TTCGTCGGAGACGGGAACAACGTCTGTTCGTCCCTGGCCCTCGTGTGTGCCACTTTGGGTTGGGATTTCGTGCACGCAGT
GCCCGAAGGGTATGAGTGCCCGGACAGAGTTTGGCGGGAGGTAGAGCGTCGCGCCGAGGAATCGGGGTCGGAGACCCGGG
TAGTTCGAGATCCTAAGGAGGCCGTACGCGAAGCCGACGTCGTGTACACCGACGTGTGGGTGAGCATGGGCGACGAAGCC
GAACGGGAGGAACGATTGCGGGTATTCCGGCCGTATCAGGTCAACGAGGAATTAATGTCACACGCTCCCGAGCACGCGAT
CGTCATGCACTGCATGCCCATACAACGTGGGTACGAGCTCACGGACGACGTGGCGGACTCGGAGCGGAGCGTGATCTACG
ACCAGGCTGAAAACCGCTTGCACGTGCAGAAAGCCATCTTAGCTCTCCTCATGGGGTGA

Upstream 100 bases:

>100_bases
AGTGCGTGAGCGGGGAAAGGATTCGGCACCGGTCAGACATCGGAAAGCATGAGACCGTTGAGAAGCGGGTGCGCCGTATG
AAGCGAATCCGTGGGGAGTA

Downstream 100 bases:

>100_bases
CCTGAACAGTGTCCAAAGTGGCCGTGATCGGTGCGACCGGACGTGTCGGTTCGACCGCGGCCGCCCGCCTCGCGTTACTG
GACTGCGTGAACGAGGTCAC

Product: ornithine carbamoyltransferase

Products: NA

Alternate protein names: OTCase

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPSTRTRVSFEVAMHQLGGQAFTY
TKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEFARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVA
FVGDGNNVCSSLALVCATLGWDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEA
EREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRLHVQKAILALLMG

Sequences:

>Translated_312_residues
MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPSTRTRVSFEVAMHQLGGQAFTY
TKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEFARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVA
FVGDGNNVCSSLALVCATLGWDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEA
EREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRLHVQKAILALLMG
>Mature_312_residues
MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPSTRTRVSFEVAMHQLGGQAFTY
TKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEFARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVA
FVGDGNNVCSSLALVCATLGWDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEA
EREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRLHVQKAILALLMG

Specific function: Arginine biosynthesis; sixth step. [C]

COG id: COG0078

COG function: function code E; Ornithine carbamoyltransferase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI38788445, Length=309, Percent_Identity=36.5695792880259, Blast_Score=214, Evalue=7e-56,
Organism=Homo sapiens, GI18105007, Length=315, Percent_Identity=29.8412698412698, Blast_Score=124, Evalue=2e-28,
Organism=Escherichia coli, GI1786469, Length=334, Percent_Identity=35.0299401197605, Blast_Score=188, Evalue=4e-49,
Organism=Escherichia coli, GI1790703, Length=323, Percent_Identity=35.6037151702786, Blast_Score=187, Evalue=1e-48,
Organism=Escherichia coli, GI2367364, Length=316, Percent_Identity=29.4303797468354, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI48994908, Length=342, Percent_Identity=24.2690058479532, Blast_Score=78, Evalue=8e-16,
Organism=Caenorhabditis elegans, GI193204318, Length=327, Percent_Identity=29.9694189602446, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6322373, Length=322, Percent_Identity=33.5403726708075, Blast_Score=195, Evalue=8e-51,
Organism=Saccharomyces cerevisiae, GI6322331, Length=314, Percent_Identity=32.1656050955414, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=29.5238095238095, Blast_Score=123, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): OTC_METKA (Q8TWG4)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614353.1
- ProteinModelPortal:   Q8TWG4
- SMR:   Q8TWG4
- GeneID:   1477171
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1070
- NMPDR:   fig|190192.1.peg.1066
- HOGENOM:   HBG579429
- OMA:   ARRHEDI
- BioCyc:   MKAN190192:MK1070-MONOMER
- BRENDA:   2.1.3.3
- HAMAP:   MF_01109
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002292
- PRINTS:   PR00100
- PRINTS:   PR00102
- TIGRFAMs:   TIGR00658

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.3

Molecular weight: Translated: 35495; Mature: 35495

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: BINDING 110-110 BINDING 137-137

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPST
CCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCC
RTRVSFEVAMHQLGGQAFTYTKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEF
CHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVAFVGDGNNVCSSLALVCATLG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
WDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHEEEHHHHHCCCHH
EREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRL
HHHHHHHHCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHCCHH
HVQKAILALLMG
HHHHHHHHHHCC
>Mature Secondary Structure
MRLKRLSTNHLLSIADLDREDVETVLRVAERFKERYLAGERVIPILEGKTLGLIFEKPST
CCCCCCCCCCEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEEEEEECCCC
RTRVSFEVAMHQLGGQAFTYTKQELQLGRGEAIKDTAAVLSRYLDGVMIRARRHEDIEEF
CHHHHHHHHHHHHCCCCEEEEHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHH
ARYSEVPVINGLSDLEHPCQALTDAFTIREKLGRGPHTVAFVGDGNNVCSSLALVCATLG
HHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHH
WDFVHAVPEGYECPDRVWREVERRAEESGSETRVVRDPKEAVREADVVYTDVWVSMGDEA
HHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHEEEHHHHHCCCHH
EREERLRVFRPYQVNEELMSHAPEHAIVMHCMPIQRGYELTDDVADSERSVIYDQAENRL
HHHHHHHHCCCCCCCHHHHHCCCCCEEEEEEEECCCCCCCCHHHCCCCHHHHHHHHCCHH
HVQKAILALLMG
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014