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The map label for this gene is PyrE

Identifier: 20094508

GI number: 20094508

Start: 1039833

End: 1040384

Strand: Reverse

Name: PyrE

Synonym: MK1072

Alternate gene names: 20094508

Gene position: 1040384-1039833 (Counterclockwise)

Preceding gene: 20094509

Following gene: 20094507

Centisome position: 61.38

GC content: 61.96

Gene sequence:

>552_bases
ATGGTGATGAATCGGGAGAGACTCGCCGAGATGTTGTTGGAGGTAGGGGCACTGAAGTTCGGGGATTTCGTGCTCTCCTC
GGGTAAGCGAAGTGACTATTACGTAGATATCAAGGAGGCCTGCACGCACCCGAAGGTTCTTGACGCGCTCACGGACGCCC
TCCTCGAGGTCCTACCGGATGGAGATGTACTCGCTGGACCGGAGCTCGGAGCGGTTCCGCTAGTCTCGGTGCTCTCGGTG
AAGGCCGGATTACCCATGGCGATAGTTCGGAAGCGGAAGAAGGAGTACGGAACCGGGGAGCGGATCGTAGGGGACGTGAG
GGGCCGGAAGGTCGTACTCGTCGACGACGTCGCGACGACCGGAGGGTCACTCTTGGAGGCCTTAGAAGCCATCGAAGAGG
AGGGCGGAGAGGTGCGGGACGCCGTCGTCGTGGTCGATCGACAGGAGGGGGCGGAAGAAGCGCTTAAAGAGCGGGGTGTG
AGGCTCTCGAGCGTGCTGACGGCCGACGACCTGCGGGGGCTGCGGGATGCTGAAAGTACTGCTCGTGGGTAG

Upstream 100 bases:

>100_bases
ACCCACCGGGTTGGGTGCTAGTCGGTCTGGCCATCCTGACCCTGGCGCTCCACTGGCTGACCAACGTCATCGGATACCTC
CTGAAGCTGAAGGAGGTGCC

Downstream 100 bases:

>100_bases
CGGCGCCCGAGAACACGCGATCGCAGAGGCGCTTTGCGGAGCTCCGGACGAGGACGTAGAGCTTTACGCGTTCATGGGGA
ACCGGAACCCGGGTATCATC

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPDGDVLAGPELGAVPLVSVLSV
KAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATTGGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGV
RLSSVLTADDLRGLRDAESTARG

Sequences:

>Translated_183_residues
MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPDGDVLAGPELGAVPLVSVLSV
KAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATTGGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGV
RLSSVLTADDLRGLRDAESTARG
>Mature_183_residues
MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPDGDVLAGPELGAVPLVSVLSV
KAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATTGGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGV
RLSSVLTADDLRGLRDAESTARG

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP)

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily

Homologues:

Organism=Homo sapiens, GI4507835, Length=161, Percent_Identity=37.2670807453416, Blast_Score=117, Evalue=7e-27,
Organism=Escherichia coli, GI1790073, Length=191, Percent_Identity=28.2722513089005, Blast_Score=76, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17508631, Length=167, Percent_Identity=34.7305389221557, Blast_Score=93, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17554964, Length=169, Percent_Identity=33.1360946745562, Blast_Score=91, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6323927, Length=175, Percent_Identity=32.5714285714286, Blast_Score=82, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6323530, Length=192, Percent_Identity=28.125, Blast_Score=79, Evalue=3e-16,
Organism=Drosophila melanogaster, GI17933654, Length=165, Percent_Identity=37.5757575757576, Blast_Score=94, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PYRE_METKA (P58860)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614355.1
- ProteinModelPortal:   P58860
- SMR:   P58860
- GeneID:   1477173
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1072
- NMPDR:   fig|190192.1.peg.1068
- HOGENOM:   HBG404341
- OMA:   LSYPRIR
- BioCyc:   MKAN190192:MK1072-MONOMER
- BRENDA:   2.4.2.10
- HAMAP:   MF_01208
- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836
- TIGRFAMs:   TIGR00336

Pfam domain/function: PF00156 Pribosyltran

EC number: =2.4.2.10

Molecular weight: Translated: 19601; Mature: 19601

Theoretical pI: Translated: 4.54; Mature: 4.54

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: BINDING 90-90 BINDING 91-91 BINDING 94-94 BINDING 119-119 BINDING 147-147

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCC
GDVLAGPELGAVPLVSVLSVKAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATT
CCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCC
GGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGVRLSSVLTADDLRGLRDAEST
CHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCHHHHHHCHHHHHHHHHHHHH
ARG
CCC
>Mature Secondary Structure
MVMNRERLAEMLLEVGALKFGDFVLSSGKRSDYYVDIKEACTHPKVLDALTDALLEVLPD
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCCC
GDVLAGPELGAVPLVSVLSVKAGLPMAIVRKRKKEYGTGERIVGDVRGRKVVLVDDVATT
CCEECCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHCCCCCEEEEEECCCCC
GGSLLEALEAIEEEGGEVRDAVVVVDRQEGAEEALKERGVRLSSVLTADDLRGLRDAEST
CHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHCCCHHHHHHCHHHHHHHHHHHHH
ARG
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014