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The map label for this gene is Ldh

Identifier: 20094505

GI number: 20094505

Start: 1036609

End: 1037562

Strand: Reverse

Name: Ldh

Synonym: MK1069

Alternate gene names: 20094505

Gene position: 1037562-1036609 (Counterclockwise)

Preceding gene: 20094506

Following gene: 20094504

Centisome position: 61.21

GC content: 59.54

Gene sequence:

>954_bases
GTGTCCAAAGTGGCCGTGATCGGTGCGACCGGACGTGTCGGTTCGACCGCGGCCGCCCGCCTCGCGTTACTGGACTGCGT
GAACGAGGTCACCCTCATCGCCAGGCCTAAAAGCGTGGATAAGCTGCGCGGGCTCCGCCGTGATATCCTGGACTCGTTAG
CGGCCGCACAAAAAGACGCCGAGATCACGATCGGGTGCGAGCGAGACGACTACGTCGATGCGGACGTCATCGTGATGACG
GCGGGGATCCCGAGGAAGCCCGGCCAGACCCGTCTCGACCTCACCAAGGATAACGCCGCGATCATCAAGAAGTACCTGGA
AGGTGTTGCGGAGGAGAACCCCGAGGCAATAGTGCTCGTGGTTACGAACCCCGTCGACGTGCTTACTTACGTGGCTCTGA
AGGTCTCCGGGTTACCTAAGAATCGCGTGATCGGCCTCGGTACTCACCTTGACTCTATGCGGTTCAAAGTTCTAATTGCC
AAACATTTCAACGTGCACATGAGTGAAGTGCACACTCGCATAATCGGCGAGCACGGCGACACCATGGTACCGGTGATCAG
TTCCACGAGCGTCGGAGGTATCCCGGTCACCAGGATGCCGGGGTGGGAGGATTTCGACGTGGAAGAAGCCGTGAGGGAAG
TGAAGGAAGCCGGTCAGCGCATCATCGAGACCTGGGGAGGATCACAGTTCGGACCGGCGCAGGCGATCACCAACCTCGTC
CGGACGATACTGCAGGACGAACGCCGCGTGCTGACGGTCTCCGCGTACCTGGACGGGGAGATCGACGGTATCCGCGACGT
CTGCATCGGGGTCCCAGCCAGACTGGGCCGGGAGGGTGTCCTAGAGATAGTACCGATAGAGCTCGAAGAAGACGAGATGA
GAGCGTTCAGACGGTCGGTTAAAGTCGTTAAGGAGGCTACGAGAGAGGCGATGGAAGCGATTTCTGAAAGATGA

Upstream 100 bases:

>100_bases
ACGTGGCGGACTCGGAGCGGAGCGTGATCTACGACCAGGCTGAAAACCGCTTGCACGTGCAGAAAGCCATCTTAGCTCTC
CTCATGGGGTGACCTGAACA

Downstream 100 bases:

>100_bases
TAAAGGAGGGCGGAAGTTGTTTCGGGTCCGTAACGAGCGGCACGAGCGGTACCTGGCGGTAGCGGTCCCCCTCCTTGCAG
CGGTCTTCCTCCTGACAACC

Product: malate dehydrogenase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDAEITIGCERDDYVDADVIVMT
AGIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLVVTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIA
KHFNVHMSEVHTRIIGEHGDTMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLV
RTILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSVKVVKEATREAMEAISER

Sequences:

>Translated_317_residues
MSKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDAEITIGCERDDYVDADVIVMT
AGIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLVVTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIA
KHFNVHMSEVHTRIIGEHGDTMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLV
RTILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSVKVVKEATREAMEAISER
>Mature_316_residues
SKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDAEITIGCERDDYVDADVIVMTA
GIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLVVTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIAK
HFNVHMSEVHTRIIGEHGDTMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLVR
TILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSVKVVKEATREAMEAISER

Specific function: Catalyzes the reversible oxidation of malate to oxaloacetate

COG id: COG0039

COG function: function code C; Malate/lactate dehydrogenases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LDH/MDH superfamily. MDH type 3 family

Homologues:

Organism=Homo sapiens, GI15082234, Length=311, Percent_Identity=32.475884244373, Blast_Score=171, Evalue=7e-43,
Organism=Homo sapiens, GI47059044, Length=323, Percent_Identity=32.1981424148607, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI221136809, Length=323, Percent_Identity=32.1981424148607, Blast_Score=170, Evalue=1e-42,
Organism=Homo sapiens, GI9257228, Length=318, Percent_Identity=32.7044025157233, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI4504973, Length=318, Percent_Identity=32.7044025157233, Blast_Score=169, Evalue=2e-42,
Organism=Homo sapiens, GI5031857, Length=304, Percent_Identity=33.8815789473684, Blast_Score=161, Evalue=7e-40,
Organism=Homo sapiens, GI260099723, Length=314, Percent_Identity=33.1210191082803, Blast_Score=161, Evalue=8e-40,
Organism=Homo sapiens, GI291575128, Length=306, Percent_Identity=31.3725490196078, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI4557032, Length=306, Percent_Identity=31.3725490196078, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI260099727, Length=222, Percent_Identity=34.2342342342342, Blast_Score=133, Evalue=2e-31,
Organism=Homo sapiens, GI260099725, Length=198, Percent_Identity=36.3636363636364, Blast_Score=130, Evalue=2e-30,
Organism=Homo sapiens, GI207028494, Length=196, Percent_Identity=36.2244897959184, Blast_Score=118, Evalue=9e-27,
Organism=Homo sapiens, GI103472011, Length=204, Percent_Identity=25.9803921568627, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI21735621, Length=304, Percent_Identity=28.2894736842105, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI103472015, Length=95, Percent_Identity=32.6315789473684, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789632, Length=223, Percent_Identity=33.6322869955157, Blast_Score=87, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17535107, Length=279, Percent_Identity=33.6917562724014, Blast_Score=147, Evalue=7e-36,
Organism=Caenorhabditis elegans, GI17554310, Length=305, Percent_Identity=28.5245901639344, Blast_Score=82, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6320125, Length=292, Percent_Identity=27.0547945205479, Blast_Score=79, Evalue=7e-16,
Organism=Saccharomyces cerevisiae, GI6322765, Length=303, Percent_Identity=27.0627062706271, Blast_Score=76, Evalue=6e-15,
Organism=Drosophila melanogaster, GI17136226, Length=308, Percent_Identity=32.4675324675325, Blast_Score=156, Evalue=2e-38,
Organism=Drosophila melanogaster, GI45550422, Length=313, Percent_Identity=27.4760383386581, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24647881, Length=326, Percent_Identity=28.2208588957055, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24663599, Length=254, Percent_Identity=26.7716535433071, Blast_Score=85, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24663595, Length=255, Percent_Identity=23.921568627451, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: 2640 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2380 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 260 Molecules/Cell In: Stationary Phase

Swissprot (AC and ID): MDH_METKA (Q8TWG5)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614352.1
- ProteinModelPortal:   Q8TWG5
- GeneID:   1477170
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK1069
- NMPDR:   fig|190192.1.peg.1065
- HOGENOM:   HBG566126
- OMA:   TIRIDES
- ProtClustDB:   PRK06223
- BioCyc:   MKAN190192:MK1069-MONOMER
- BRENDA:   1.1.1.37
- GO:   GO:0005737
- GO:   GO:0005488
- GO:   GO:0006096
- HAMAP:   MF_00487
- InterPro:   IPR001557
- InterPro:   IPR018177
- InterPro:   IPR022383
- InterPro:   IPR001236
- InterPro:   IPR015955
- InterPro:   IPR011275
- InterPro:   IPR016040
- Gene3D:   G3DSA:3.90.110.10
- Gene3D:   G3DSA:3.40.50.720
- PIRSF:   PIRSF000102
- PRINTS:   PR00086

Pfam domain/function: PF02866 Ldh_1_C; PF00056 Ldh_1_N; SSF56327 Lactate_DH/Glyco_hydro_4_C

EC number: =1.1.1.37

Molecular weight: Translated: 34609; Mature: 34478

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00064 L_LDH

Important sites: ACT_SITE 178-178 BINDING 85-85 BINDING 91-91 BINDING 98-98 BINDING 123-123 BINDING 154-154

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDA
CCCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
EITIGCERDDYVDADVIVMTAGIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLV
EEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
VTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIAKHFNVHMSEVHTRIIGEHGD
EECCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHEEEEEEHHCCHHHHHHHHHHHCCCCC
TMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLV
EEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RTILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCHHCCCCCCEEEEEEEECHHHHHHHHHHH
KVVKEATREAMEAISER
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SKVAVIGATGRVGSTAAARLALLDCVNEVTLIARPKSVDKLRGLRRDILDSLAAAQKDA
CCEEEEECCCCCCCHHHHHHHHHHHHHHHEEEECCCHHHHHHHHHHHHHHHHHHHCCCC
EITIGCERDDYVDADVIVMTAGIPRKPGQTRLDLTKDNAAIIKKYLEGVAEENPEAIVLV
EEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEECCCCHHHHHHHHHHHHCCCCCEEEEE
VTNPVDVLTYVALKVSGLPKNRVIGLGTHLDSMRFKVLIAKHFNVHMSEVHTRIIGEHGD
EECCHHHHHHHHHHCCCCCCCCEEEECCCCCCHHEEEEEEHHCCHHHHHHHHHHHCCCCC
TMVPVISSTSVGGIPVTRMPGWEDFDVEEAVREVKEAGQRIIETWGGSQFGPAQAITNLV
EEEEEEECCCCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHH
RTILQDERRVLTVSAYLDGEIDGIRDVCIGVPARLGREGVLEIVPIELEEDEMRAFRRSV
HHHHHCCCEEEEEEEEECCCCCCHHHHHHCCCHHCCCCCCEEEEEEEECHHHHHHHHHHH
KVVKEATREAMEAISER
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014