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The map label for this gene is cofE

Identifier: 20094407

GI number: 20094407

Start: 930168

End: 930926

Strand: Direct

Name: cofE

Synonym: MK0971

Alternate gene names: 20094407

Gene position: 930168-930926 (Clockwise)

Preceding gene: 20094405

Following gene: 20094408

Centisome position: 54.88

GC content: 63.9

Gene sequence:

>759_bases
ATGGGGGGACTCCCCACGCTTCGCATCGAGCCGGTTCCGCTGGAGAGGAAGGTACGACCCGGAGACGACCTCGCGGAGCT
CATCGCGGAGTCCGCGGAGCTCGAGGAGGGGGACGTCCTCGCGATCGCTCACACGGTAGTATCTAAGGCCGAGGGGGCCC
TAATTAGTCTCGACGAGATCGAGCCCAGTCCCTTCGCTAAGACCCTCGCCGAGCGGACTGGTAAAGATCCGCGTGTGGTC
GAGGTGATCCTTCGGGAAGCGGAGTCCATCGTGCGGGTCGGTCCGGACTTCATCATCACGGAAGTGCGAGGAGGGATGGT
CTGCGCCAACGCAGGCGTCGACGAATCGAACGCACCGCCCGGGTACGTGATCGTCCTGCCCGAGGATCCCGATCGCAGCG
CCCGAGAACTCCGTCGGCGTCTCCGCGAACTCGTCGGAGTTGACGTAGGAGTCATCATCACCGACACTCAAGGTCGACCT
TTCAGGGAAGGGGTTGTCGGCGTAGCGATCGGGGCCTCCGGAGTGCCCGTACTGGCCGACCGGCGTGGGGACCGCGATCT
CTACGGTCGCGAGCTGAAAATCACGATCGTAGCGCTCGGTGACCTCCTAGCCTCCGCGGCGGAGCTCGTGATGGGGCAGG
CCGACGAGGGTACGCCTGCGGTCATCTTCCGTGGGCTCAAGCCCGAGCTTGAGCGGTTCGAAGGTCCCCGGAAGGCTCGA
GCGATTATCCGCTCTCCAAGTAGGGATATATTCAGATGA

Upstream 100 bases:

>100_bases
CGCCTCGATCATAGCGCGGATAGCGTTCCTACGCGCTTCCTCCGCCAGCTTGGAGGACGGGTACAGTGTGTCGTCCACAT
CGAACAGTACGGCTTTGATC

Downstream 100 bases:

>100_bases
ACAAACATTTGGCCCACACAAACCTCACGTGGGTTATCTCACCAAAAAGTATTTAACTAATTGTGGTTTTCAGTGAGTTC
TGGATAGTACGATGATACCC

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 252; Mature: 251

Protein sequence:

>252_residues
MGGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEIEPSPFAKTLAERTGKDPRVV
EVILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPPGYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRP
FREGVVGVAIGASGVPVLADRRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKAR
AIIRSPSRDIFR

Sequences:

>Translated_252_residues
MGGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEIEPSPFAKTLAERTGKDPRVV
EVILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPPGYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRP
FREGVVGVAIGASGVPVLADRRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKAR
AIIRSPSRDIFR
>Mature_251_residues
GGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEIEPSPFAKTLAERTGKDPRVVE
VILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPPGYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRPF
REGVVGVAIGASGVPVLADRRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKARA
IIRSPSRDIFR

Specific function: Catalyzes the GTP-dependent successive addition of multiple gamma-linked L-glutamates to the L-lactyl phosphodiester of 7,8-didemethyl-8-hydroxy-5-deazariboflavin (F420-0) to form polyglutamated F420 derivatives

COG id: COG1478

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CofE family

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): COFE_METKA (Q8TWR1)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_614254.1
- ProteinModelPortal:   Q8TWR1
- SMR:   Q8TWR1
- GeneID:   1477072
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0971
- NMPDR:   fig|190192.1.peg.967
- HOGENOM:   HBG578188
- OMA:   LVMGQAD
- BioCyc:   MKAN190192:MK0971-MONOMER
- HAMAP:   MF_01258
- InterPro:   IPR002847
- InterPro:   IPR008225
- TIGRFAMs:   TIGR01916

Pfam domain/function: PF01996 F420_ligase

EC number: NA

Molecular weight: Translated: 27136; Mature: 27004

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEI
CCCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCEEEECCC
EPSPFAKTLAERTGKDPRVVEVILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPP
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCC
GYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRPFREGVVGVAIGASGVPVLAD
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCEEEEEECCCCCCEEEC
RRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKAR
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCHHHHH
AIIRSPSRDIFR
HHHCCCCHHHCC
>Mature Secondary Structure 
GGLPTLRIEPVPLERKVRPGDDLAELIAESAELEEGDVLAIAHTVVSKAEGALISLDEI
CCCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCCEEEECCC
EPSPFAKTLAERTGKDPRVVEVILREAESIVRVGPDFIITEVRGGMVCANAGVDESNAPP
CCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEECCCCCCCCCCC
GYVIVLPEDPDRSARELRRRLRELVGVDVGVIITDTQGRPFREGVVGVAIGASGVPVLAD
CEEEEECCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCHHHCEEEEEECCCCCCEEEC
RRGDRDLYGRELKITIVALGDLLASAAELVMGQADEGTPAVIFRGLKPELERFEGPRKAR
CCCCCCCCCCEEEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEECCCHHHHHCCCHHHHH
AIIRSPSRDIFR
HHHCCCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11930014