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The map label for this gene is sdhA [C]

Identifier: 20094264

GI number: 20094264

Start: 785987

End: 787657

Strand: Direct

Name: sdhA [C]

Synonym: MK0828

Alternate gene names: 20094264

Gene position: 785987-787657 (Clockwise)

Preceding gene: 20094263

Following gene: 20094270

Centisome position: 46.37

GC content: 60.32

Gene sequence:

>1671_bases
TTGGAGCATCTGGAATGTGACGTGCTCGTGATCGGAGGTGGTGGTGCGGCCGCCCGTGCGGCGATCGAGGCCGCTAGGAG
GAATCTGAACGTGGTGATCGTCTCCAAAGGTGCCGTGGGTAGAAGTGGATGCACCGTCATGGCGGAAGGGGGCTACAACG
CGGTCTTACGCACGGCCGATCCGGATGACTCCTTCGACGCTCACTTCCGCGACACCGTCGAGGGAGGCGCCTACCTCAAC
GATCAGGACATGGTAGAAATCCTCGTACGAGAAGCACCTAAGCGCTTACTCGACCTAGAGAACTTCGGAGCCGTGTTCGA
CCGTAACGAGGACGGTACCCTAGCCCAGCGTCCTTTCGGTGGTCAATCTAAGCCCCGTACTTGCTACGCCGGTGACCGTA
CGGGACACGAGATAATGATGGCGTTGCTGGACGAAGTTCGCCGCCTCGACGGTATCACCGTTCTGGAACGCACAATGGGA
TACGGACTGGTTCGAGATCACGACGGAAGGGTAGTGGGCGCGGTATGCGTGGACCTTCAAACCGAGGATACCATCGTCAT
CACGGCCTACGCCACCGTTCTAGCTACAGGGGGCGCGGGTCAGCTCTACCCCGTCACCACGAACCCCGTCCACAAGACCG
GTGACGGGTACGCCATGGCCTTACGCGTCGGTTGCCCGCTCATTGACATGGAAATGGTTCAGTTTCACCCCACTGGAATG
GTGTATCCCGAGAGCGTTCGCGGAGTTTTGGTTACGGAAGCCGTTAGAGGTGAGGGAGGACGGCTTTACAACGCCAGAGG
CGAGCGGTTCATGAAGCGCTACGATCCCGAGAGGATGGAGCTGGCGACGCGCGACGTCGTAGCTCGGGCTATTTACAGGG
AGATCAAAGAGGGACGTGGGACGGAGCATGGTGGAGTTTACCTGGACGTCACTCACCTGCCCGACGAGGTGATCGAGGAG
AAGCTCGAGACTACGCTCAAGCAGTTCCTGCGCGTGGGCGTGGACATTCGAAACGAACCCATGGAGGTGGCTCCCACGGC
CCATCACTTCATGGGAGGGGTCCCGATCGACGCCGACGGGAAGACGCCGATCCCGGGTCTCTTCGCGTGCGGTGAGGTTA
CGGGAGGTATCCACGGAGCGAACCGACTCGGCGGAAACGCCCTGGCCGATACACAGGTTTTCGGGTACCGCGCCGGAAGG
GCCGCCGCCGAGCTCGCCTTGAAGTTACGGAGACGAGGTCGTAGGATACAGGTGCGCAAGACCGATGCGCCGACGGTGGA
GTCCGAGATTTGGTCCACCGTCGGTCAAGAGGATCCGATTCGAGTGCGCGAGAAGCTCCGGGAGACGATGTGGGAATACG
TGGGTCTGGAGAGGTCCGAGAAAGGTCTCAAGACGGCGATCAAAACCCTCAACGAGCTCAAGGAGACCGTGGAATCCGGT
CTAGACGATGAATACGGGTTACGGCCGGTCTTAGAGGTGCGCAACATGGTAGAGGTGGCTATCGCCGTCGCGCGATCCGC
CCTTTATCGGACCGAGAGCCGTGGAGCTCACTATCGGTCGGATTATCCCGAACGCGATGACAAACGGTGGCTTCGTCACA
CTGTCTACAGGCCCAACGGCTCTCTGGCCACCCGTCCCGCTAAGATCACTCGACTGGATCCCCGTCAGTGA

Upstream 100 bases:

>100_bases
CCGACGTCGTGCGTTTCCGGGTCACACTTTCCAATAGGGATGAGTTGTCCGACAGAATCCTCGAGCACGTGCTTGAGTGG
TTGGAGGAGAGGTGATCGTT

Downstream 100 bases:

>100_bases
TTATGTCGACGAAGCGGATCTCGTCCGGTTCTACCTTCACTAACCCTCGATCCGTGAGCCTCAGTTCCGGGACCACCGGA
AGGGTCACGAAAGATAGAGC

Product: succinate dehydrogenase flavoprotein subunit

Products: fumarate; reduced acceptor

Alternate protein names: NA

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MEHLECDVLVIGGGGAAARAAIEAARRNLNVVIVSKGAVGRSGCTVMAEGGYNAVLRTADPDDSFDAHFRDTVEGGAYLN
DQDMVEILVREAPKRLLDLENFGAVFDRNEDGTLAQRPFGGQSKPRTCYAGDRTGHEIMMALLDEVRRLDGITVLERTMG
YGLVRDHDGRVVGAVCVDLQTEDTIVITAYATVLATGGAGQLYPVTTNPVHKTGDGYAMALRVGCPLIDMEMVQFHPTGM
VYPESVRGVLVTEAVRGEGGRLYNARGERFMKRYDPERMELATRDVVARAIYREIKEGRGTEHGGVYLDVTHLPDEVIEE
KLETTLKQFLRVGVDIRNEPMEVAPTAHHFMGGVPIDADGKTPIPGLFACGEVTGGIHGANRLGGNALADTQVFGYRAGR
AAAELALKLRRRGRRIQVRKTDAPTVESEIWSTVGQEDPIRVREKLRETMWEYVGLERSEKGLKTAIKTLNELKETVESG
LDDEYGLRPVLEVRNMVEVAIAVARSALYRTESRGAHYRSDYPERDDKRWLRHTVYRPNGSLATRPAKITRLDPRQ

Sequences:

>Translated_556_residues
MEHLECDVLVIGGGGAAARAAIEAARRNLNVVIVSKGAVGRSGCTVMAEGGYNAVLRTADPDDSFDAHFRDTVEGGAYLN
DQDMVEILVREAPKRLLDLENFGAVFDRNEDGTLAQRPFGGQSKPRTCYAGDRTGHEIMMALLDEVRRLDGITVLERTMG
YGLVRDHDGRVVGAVCVDLQTEDTIVITAYATVLATGGAGQLYPVTTNPVHKTGDGYAMALRVGCPLIDMEMVQFHPTGM
VYPESVRGVLVTEAVRGEGGRLYNARGERFMKRYDPERMELATRDVVARAIYREIKEGRGTEHGGVYLDVTHLPDEVIEE
KLETTLKQFLRVGVDIRNEPMEVAPTAHHFMGGVPIDADGKTPIPGLFACGEVTGGIHGANRLGGNALADTQVFGYRAGR
AAAELALKLRRRGRRIQVRKTDAPTVESEIWSTVGQEDPIRVREKLRETMWEYVGLERSEKGLKTAIKTLNELKETVESG
LDDEYGLRPVLEVRNMVEVAIAVARSALYRTESRGAHYRSDYPERDDKRWLRHTVYRPNGSLATRPAKITRLDPRQ
>Mature_556_residues
MEHLECDVLVIGGGGAAARAAIEAARRNLNVVIVSKGAVGRSGCTVMAEGGYNAVLRTADPDDSFDAHFRDTVEGGAYLN
DQDMVEILVREAPKRLLDLENFGAVFDRNEDGTLAQRPFGGQSKPRTCYAGDRTGHEIMMALLDEVRRLDGITVLERTMG
YGLVRDHDGRVVGAVCVDLQTEDTIVITAYATVLATGGAGQLYPVTTNPVHKTGDGYAMALRVGCPLIDMEMVQFHPTGM
VYPESVRGVLVTEAVRGEGGRLYNARGERFMKRYDPERMELATRDVVARAIYREIKEGRGTEHGGVYLDVTHLPDEVIEE
KLETTLKQFLRVGVDIRNEPMEVAPTAHHFMGGVPIDADGKTPIPGLFACGEVTGGIHGANRLGGNALADTQVFGYRAGR
AAAELALKLRRRGRRIQVRKTDAPTVESEIWSTVGQEDPIRVREKLRETMWEYVGLERSEKGLKTAIKTLNELKETVESG
LDDEYGLRPVLEVRNMVEVAIAVARSALYRTESRGAHYRSDYPERDDKRWLRHTVYRPNGSLATRPAKITRLDPRQ

Specific function: Two Distinct, Membrane-Bound, FAD-Containing Enzymes Are Responsible For The Catalysis Of Fumarate And Succinate Interconversion; The Fumarate Reductase Is Used In Anaerobic Growth, And The Succinate Dehydrogenase Is Used In Aerobic Growth. [C]

COG id: COG1053

COG function: function code C; Succinate dehydrogenase/fumarate reductase, flavoprotein subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FAD-dependent oxidoreductase 2 family. FRD/SDH subfamily [H]

Homologues:

Organism=Homo sapiens, GI156416003, Length=539, Percent_Identity=37.291280148423, Blast_Score=287, Evalue=2e-77,
Organism=Escherichia coli, GI1786942, Length=590, Percent_Identity=38.9830508474576, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI1788928, Length=534, Percent_Identity=37.6404494382022, Blast_Score=296, Evalue=2e-81,
Organism=Escherichia coli, GI1790597, Length=555, Percent_Identity=37.8378378378378, Blast_Score=282, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17505833, Length=519, Percent_Identity=38.5356454720617, Blast_Score=298, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17550100, Length=588, Percent_Identity=35.5442176870748, Blast_Score=289, Evalue=3e-78,
Organism=Caenorhabditis elegans, GI71986328, Length=473, Percent_Identity=28.5412262156448, Blast_Score=114, Evalue=1e-25,
Organism=Saccharomyces cerevisiae, GI6322701, Length=563, Percent_Identity=38.5435168738899, Blast_Score=326, Evalue=5e-90,
Organism=Saccharomyces cerevisiae, GI6322416, Length=535, Percent_Identity=37.9439252336449, Blast_Score=317, Evalue=3e-87,
Organism=Saccharomyces cerevisiae, GI6320788, Length=476, Percent_Identity=29.6218487394958, Blast_Score=114, Evalue=4e-26,
Organism=Saccharomyces cerevisiae, GI6322511, Length=484, Percent_Identity=27.4793388429752, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI17137288, Length=516, Percent_Identity=37.984496124031, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655642, Length=516, Percent_Identity=37.984496124031, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24655647, Length=516, Percent_Identity=37.984496124031, Blast_Score=290, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24663005, Length=607, Percent_Identity=34.7611202635914, Blast_Score=266, Evalue=4e-71,

Paralogues:

None

Copy number: 1900 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1360 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 1100 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003953
- InterPro:   IPR013027
- InterPro:   IPR015939
- InterPro:   IPR004112 [H]

Pfam domain/function: PF00890 FAD_binding_2; PF02910 Succ_DH_flav_C [H]

EC number: 1.3.99.1

Molecular weight: Translated: 61374; Mature: 61374

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEHLECDVLVIGGGGAAARAAIEAARRNLNVVIVSKGAVGRSGCTVMAEGGYNAVLRTAD
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCEEEEECC
PDDSFDAHFRDTVEGGAYLNDQDMVEILVREAPKRLLDLENFGAVFDRNEDGTLAQRPFG
CCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCHHHCCHHEECCCCCCEEECCCC
GQSKPRTCYAGDRTGHEIMMALLDEVRRLDGITVLERTMGYGLVRDHDGRVVGAVCVDLQ
CCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCEEEEEEEEEEC
TEDTIVITAYATVLATGGAGQLYPVTTNPVHKTGDGYAMALRVGCPLIDMEMVQFHPTGM
CCCEEEEEEEEHHHEECCCCEEEEECCCCCCCCCCCEEEEEEECCCEEEEHHHEECCCCC
VYPESVRGVLVTEAVRGEGGRLYNARGERFMKRYDPERMELATRDVVARAIYREIKEGRG
CCHHHCCCEEEEHHHCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
TEHGGVYLDVTHLPDEVIEEKLETTLKQFLRVGVDIRNEPMEVAPTAHHFMGGVPIDADG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
KTPIPGLFACGEVTGGIHGANRLGGNALADTQVFGYRAGRAAAELALKLRRRGRRIQVRK
CCCCCCEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
TDAPTVESEIWSTVGQEDPIRVREKLRETMWEYVGLERSEKGLKTAIKTLNELKETVESG
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LDDEYGLRPVLEVRNMVEVAIAVARSALYRTESRGAHYRSDYPERDDKRWLRHTVYRPNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECCCC
SLATRPAKITRLDPRQ
CCCCCCCEECCCCCCC
>Mature Secondary Structure
MEHLECDVLVIGGGGAAARAAIEAARRNLNVVIVSKGAVGRSGCTVMAEGGYNAVLRTAD
CCCCEEEEEEECCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCEEEECCCCCEEEEECC
PDDSFDAHFRDTVEGGAYLNDQDMVEILVREAPKRLLDLENFGAVFDRNEDGTLAQRPFG
CCCCCCCHHHHHCCCCCEECHHHHHHHHHHHHHHHHHCHHHCCHHEECCCCCCEEECCCC
GQSKPRTCYAGDRTGHEIMMALLDEVRRLDGITVLERTMGYGLVRDHDGRVVGAVCVDLQ
CCCCCCEEECCCCCHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEECCCCEEEEEEEEEEC
TEDTIVITAYATVLATGGAGQLYPVTTNPVHKTGDGYAMALRVGCPLIDMEMVQFHPTGM
CCCEEEEEEEEHHHEECCCCEEEEECCCCCCCCCCCEEEEEEECCCEEEEHHHEECCCCC
VYPESVRGVLVTEAVRGEGGRLYNARGERFMKRYDPERMELATRDVVARAIYREIKEGRG
CCHHHCCCEEEEHHHCCCCCEEECCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
TEHGGVYLDVTHLPDEVIEEKLETTLKQFLRVGVDIRNEPMEVAPTAHHFMGGVPIDADG
CCCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCCCC
KTPIPGLFACGEVTGGIHGANRLGGNALADTQVFGYRAGRAAAELALKLRRRGRRIQVRK
CCCCCCEEECCCCCCCCCCCHHCCCCCCCCCHHHCCCCCHHHHHHHHHHHHCCCEEEEEE
TDAPTVESEIWSTVGQEDPIRVREKLRETMWEYVGLERSEKGLKTAIKTLNELKETVESG
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCC
LDDEYGLRPVLEVRNMVEVAIAVARSALYRTESRGAHYRSDYPERDDKRWLRHTVYRPNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHEECCCC
SLATRPAKITRLDPRQ
CCCCCCCEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: ATP; Dithiothreitol; FAD; Fe-S-clusters [C]

Metal ions: Anions; Diphosphate; Fe [C]

Kcat value (1/min): 10000-13000 [C]

Specific activity: 509

Km value (mM): 0.4 {fumarate}} 0.02 {succinate}} 0.3 {ferricyanide}} [C]

Substrates: succinate; acceptor

Specific reaction: succinate + acceptor = fumarate + reduced acceptor

General reaction: Redox reaction [C]

Inhibitor: 5, 5'-Dithiobis (2-nitrobenzoate); Chaotropic reagents [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]