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The map label for this gene is ThiD [C]

Identifier: 20094027

GI number: 20094027

Start: 547818

End: 549116

Strand: Direct

Name: ThiD [C]

Synonym: MK0589

Alternate gene names: 20094027

Gene position: 547818-549116 (Clockwise)

Preceding gene: 20094026

Following gene: 20094028

Centisome position: 32.32

GC content: 63.97

Gene sequence:

>1299_bases
ATGCTCCTGATAGTCGCGGGACACGATCCCACGGGAGCCGGTCTCAGGGCAGACATAGCGACGGCCGAAGCCCTCGGAGT
ACCGGCGGTTTCGCTCCCGACGTTGCTATCGCTCCAGGATGAGGGCGTCGAGCTAGTCGAGCCCGTTGACTCACGGTTCT
TATCGGACTGCTTGGCCCGATACGTCCCAAGGTGCGAGGTGGTCAAAGTCGGGGCCGTTCCTACTCTGAAAACCTTCGAG
ATACTGGCGAAACGGCTCCGTGACCTGACGGTAGTGTGGGACCCCGTGTTCAGAGCCGAGGCCGGAGGGGAGTTATCCGA
AGCGACTCCTGAAGAGGCGTTGGAAACGTTTGGGGAAACAGTCGATATCCTCACTCCCAATACGGAAGAGCTGAGTCAGC
TGGTCGGAAGGGAGGTACGCACCACGGTGGAGGCCGAGCTCGCAGCCCGTGAGCTGGTGGAGGAGTACGGCCTGACGGGT
GTCCTCGTCACGGGGGGACACTCCGTCGACAGACGCGATGTGTGGGTGCCGGCTGAGGGGAACACCATCGCGTGGGAGGT
GCCTCCGGGTGAAGGCGCTCACGGGTCCGGGTGCGTGCTCTCCACGGCTCTCGCGTGCTTCTTGGTCCGCGGGTTGGACC
CTGAGACCGCCGTAGAACGAGCCGTTCGGTTCGCGAGAGCGGCGGTCCGAGAGGCCCGGAACACGCCCTTCGGCCGGGTG
GCAGATCCCGTCGCACAAATTCGACGTGACGCGACATTGGGGAAGGCAACGCAGCACGTGATCCGGGCACTCGAGATCAT
CGAGTCGGACCCGGCCTTCGCGAGGGCGATCCCACAGGTCGGAATGAACGTGGCGGAGGTCTTCGACCCCTCCCGGGGTC
TTGAAGGTGTTGTGGGACTCTCGGGTCGAATCGTCCTCGACGGTGATAGACCCACCGCGGTCGGTCGCCCGGTTCCGGGG
GGCTCCAAGCACGTCGGCACCGTGGCTTTGACGGCACATCGGCTCGACCCCTCGGTCCGGGCCGCGGTGAACGCCAGGTA
CGACGAGGAGCTTATCGAGAGGGCCCGCGATCTCGGGTTCGTAGTGTCTTCGTTCGACCGGTCGGAAGAGCCGGAGGAAG
TCGACTCCACGATGGAGTGGGGCACCGAGGTGGCGTTTCGGAGGGCGGACGACACTCCCGACGTCGTCTACGACGAGGGC
GACGTGGGTAAGGAACCGATGATACGCGTGCTCGGAAAGAGTGCCGTGGAGGCCGTGACTAAGCTGAGAATGTTGTTGGG
ATGTGTTGTTGGGGTGTAA

Upstream 100 bases:

>100_bases
GCAAAAATACCGTTCCCCAAGTCGTCGGCCCCCCGGGCTGCTGCTCGGCGTTACCCTTCATCCTAAACGTTACGAGTATT
GTGACGTTGGGGGCTCGGAT

Downstream 100 bases:

>100_bases
GGGAGTGAGCTCGTTCAAACGACTGCTTTACCTGGACGTGATCTCCCGAAGTCACGCGGATTTCGCCAAGAGGGTGATCA
GGAGGTTAGAGCCCAAGGTC

Product: fused hydroxymethylpyrimidine/phosphomethylpyrimidine kinase/uncharacterized protein

Products: ADP; 4-amino-2-methyl-5-diphosphomethylpyrimidine

Alternate protein names: NA

Number of amino acids: Translated: 432; Mature: 432

Protein sequence:

>432_residues
MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLARYVPRCEVVKVGAVPTLKTFE
ILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGETVDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTG
VLVTGGHSVDRRDVWVPAEGNTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRV
ADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGLSGRIVLDGDRPTAVGRPVPG
GSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGFVVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEG
DVGKEPMIRVLGKSAVEAVTKLRMLLGCVVGV

Sequences:

>Translated_432_residues
MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLARYVPRCEVVKVGAVPTLKTFE
ILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGETVDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTG
VLVTGGHSVDRRDVWVPAEGNTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRV
ADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGLSGRIVLDGDRPTAVGRPVPG
GSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGFVVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEG
DVGKEPMIRVLGKSAVEAVTKLRMLLGCVVGV
>Mature_432_residues
MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLARYVPRCEVVKVGAVPTLKTFE
ILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGETVDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTG
VLVTGGHSVDRRDVWVPAEGNTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRV
ADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGLSGRIVLDGDRPTAVGRPVPG
GSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGFVVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEG
DVGKEPMIRVLGKSAVEAVTKLRMLLGCVVGV

Specific function: Catalyzes The Phosphorylation Of Hmp-P To Hmp-Pp. [C]

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=29.0697674418605, Blast_Score=70, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013749
- InterPro:   IPR019293 [H]

Pfam domain/function: PF10120 MethylPyrKinase; PF08543 Phos_pyr_kin [H]

EC number: 2.7.4.7

Molecular weight: Translated: 46262; Mature: 46262

Theoretical pI: Translated: 4.46; Mature: 4.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLAR
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YVPRCEVVKVGAVPTLKTFEILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGET
HCCCCEEEEECCCCCHHHHHHHHHHHHHCEEEECCHHHHCCCCCCCCCCHHHHHHHHCCE
VDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTGVLVTGGHSVDRRDVWVPAEG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCC
NTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRV
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEECC
SGRIVLDGDRPTAVGRPVPGGSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGF
CCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
VVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEGDVGKEPMIRVLGKSAVEAVT
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
KLRMLLGCVVGV
HHHHHHHHHHCC
>Mature Secondary Structure
MLLIVAGHDPTGAGLRADIATAEALGVPAVSLPTLLSLQDEGVELVEPVDSRFLSDCLAR
CEEEEECCCCCCCCCCHHHHHHHHHCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
YVPRCEVVKVGAVPTLKTFEILAKRLRDLTVVWDPVFRAEAGGELSEATPEEALETFGET
HCCCCEEEEECCCCCHHHHHHHHHHHHHCEEEECCHHHHCCCCCCCCCCHHHHHHHHCCE
VDILTPNTEELSQLVGREVRTTVEAELAARELVEEYGLTGVLVTGGHSVDRRDVWVPAEG
EEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCEEECCCC
NTIAWEVPPGEGAHGSGCVLSTALACFLVRGLDPETAVERAVRFARAAVREARNTPFGRV
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCHH
ADPVAQIRRDATLGKATQHVIRALEIIESDPAFARAIPQVGMNVAEVFDPSRGLEGVVGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHCCCCCCCCCEECC
SGRIVLDGDRPTAVGRPVPGGSKHVGTVALTAHRLDPSVRAAVNARYDEELIERARDLGF
CCEEEEECCCCCCCCCCCCCCCCCCEEEEEEEHHCCCHHHHHHCCHHHHHHHHHHHHHHH
VVSSFDRSEEPEEVDSTMEWGTEVAFRRADDTPDVVYDEGDVGKEPMIRVLGKSAVEAVT
HHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHHHH
KLRMLLGCVVGV
HHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 4-amino-2-methyl-5-phosphomethylpyrimidine

Specific reaction: ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine

General reaction: Phospho group transfer [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]