Click here to switch to the map view.

The map label for this gene is purP

Identifier: 20094026

GI number: 20094026

Start: 546629

End: 547708

Strand: Direct

Name: purP

Synonym: MK0588

Alternate gene names: 20094026

Gene position: 546629-547708 (Clockwise)

Preceding gene: 20094025

Following gene: 20094027

Centisome position: 32.25

GC content: 59.44

Gene sequence:

>1080_bases
TTGTTATCTCGGGAGGAAATTCTCGAGATCCTCTCGGAGTACGATCTCGAGGACCTGTCCATAGCTACGCTCGGAAGCCA
TACTGCACTTCACATTCTAAAGGGCGCCAAAGAGGAAGGGCTTCGCTCTGTGGTAGTCTGTGAAGAAGGTCGCACAACAC
CCTACGAGCGACTCGGGGTCGCCGATGAGATCATCGTGGTAGAGCGCTTCCAAGACATGCTCGACGAGGAAGTTCAGGAG
CGTCTTCGAGAGTTGAACGCTATCGTCGTGCCGCACGGGTCCTTCGTCGCCTACGTGGGTCTGGACGGGATCGAAAACGA
GTTCTGCGTGCCGATGTTCGGAAACAGGCGGCTACTGCGCTGGGAGAGCGAGCGCTCTCTAGAGCGTAAGCTTCTGAAGC
GGGCGGGCGTGAAGGTGCCGAAGGTGTTCGACTCACCCGAGGACATCGACCGCCCGGTCATCGTCAAGTTCCCTGGGGCC
AGAGGGGGTCGTGGGTACTTCATCTGCTCCGATCCGGAAGAGTTCGAGGAGAAGGCAGAGCGGCTGATCGAAGACGGCGT
GATCGACGAGGAGGATCTCGAGCAGGCACACATAGAGGAGTACGTGGTCGGGACCAACTTCTGCGTCCACTACTTCCGGT
CGGTCGTGGAGGATACCGTTGAGGTACTGGGGATGGATCGCCGTTATGAGACCAACATCGATGGCCTCGTCCGGATGCCG
GCAGGCGATCAACTGGAAGCGGGACTGGAGCCTTCCTACGTCATCTCGGGTAACATCCCCGTGGTCGTGAGGGAGTCCCT
GCTGGTGCAGCTGTACGAGATGGGCGATCGCGTGGTACGCGCATCCGAGGATATCGAGGAACCCGGCTTCATCGGGCCGT
TCTGTCTACAAACGCTGTGCACCGAGGACCTCGAGTTCTACGTCTTCGAGCTCTCCGCCAGGATCGACGGTGGTACCAAC
GTGACCTTCCTGCCGTACGCGTACTTGAAGTTCGGCGAGATCGTGACCATGGGTCGCAGGATAGCCAAGGAGGTGCGCGA
GGCTCGGGACAAGGGGCTCCTCGAAGGCGTCGTTACCTGA

Upstream 100 bases:

>100_bases
CCTTTTGTGCGACTTTCATTAGAGAGTCCCGTGAGACGACACGCACCGGAGGGGGCCGGATTAAAGAAGCGTGGAGCGCG
CCCGGGCAGGGGGGCCGCCG

Downstream 100 bases:

>100_bases
CCTCTCCGGGCAAAAATACCGTTCCCCAAGTCGTCGGCCCCCCGGGCTGCTGCTCGGCGTTACCCTTCATCCTAAACGTT
ACGAGTATTGTGACGTTGGG

Product: 5-formaminoimidazole-4-carboxamide-1-(beta)-D- ribofuranosyl 5'-monophosphate synthetase

Products: NA

Alternate protein names: 5-aminoimidazole-4-carboxamide-1-beta-D-ribofuranosyl 5'-monophosphate--formate ligase

Number of amino acids: Translated: 359; Mature: 359

Protein sequence:

>359_residues
MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGVADEIIVVERFQDMLDEEVQE
RLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLRWESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGA
RGGRGYFICSDPEEFEEKAERLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMP
AGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLCTEDLEFYVFELSARIDGGTN
VTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT

Sequences:

>Translated_359_residues
MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGVADEIIVVERFQDMLDEEVQE
RLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLRWESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGA
RGGRGYFICSDPEEFEEKAERLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMP
AGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLCTEDLEFYVFELSARIDGGTN
VTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT
>Mature_359_residues
MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGVADEIIVVERFQDMLDEEVQE
RLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLRWESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGA
RGGRGYFICSDPEEFEEKAERLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMP
AGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLCTEDLEFYVFELSARIDGGTN
VTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT

Specific function: Catalyzes the ATP- and formate-dependent formylation of 5-aminoimidazole-4-carboxamide-1-beta-d-ribofuranosyl 5'- monophosphate (AICAR) to 5-formaminoimidazole-4-carboxamide-1- beta-d-ribofuranosyl 5'-monophosphate (FAICAR) in the absence of folates

COG id: COG1759

COG function: function code R; ATP-utilizing enzymes of ATP-grasp superfamily (probably carboligases)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PURP_METKA (Q8TXS2)

Other databases:

- EMBL:   AE009439
- RefSeq:   NP_613873.1
- ProteinModelPortal:   Q8TXS2
- SMR:   Q8TXS2
- GeneID:   1476689
- GenomeReviews:   AE009439_GR
- KEGG:   mka:MK0588
- NMPDR:   fig|190192.1.peg.586
- HOGENOM:   HBG392202
- OMA:   MGQRIAR
- ProtClustDB:   PRK13278
- BioCyc:   MKAN190192:MK0588-MONOMER
- HAMAP:   MF_01163
- InterPro:   IPR011761
- InterPro:   IPR013816
- InterPro:   IPR009720
- InterPro:   IPR010672
- InterPro:   IPR013817
- Gene3D:   G3DSA:3.30.470.20
- Gene3D:   G3DSA:3.40.50.20

Pfam domain/function: PF06849 DUF1246; PF06973 DUF1297

EC number: NA

Molecular weight: Translated: 40652; Mature: 40652

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS50975 ATP_GRASP

Important sites: BINDING 27-27 BINDING 94-94 BINDING 230-230 BINDING 258-258

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGV
CCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHCCEEEEEECCCCCCCHHHHCC
ADEIIVVERFQDMLDEEVQERLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCEEEEECCCCEEEE
WESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGARGGRGYFICSDPEEFEEKAE
ECHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHH
RLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
AGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLC
CCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TEDLEFYVFELSARIDGGTNVTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT
HHCHHEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLSREEILEILSEYDLEDLSIATLGSHTALHILKGAKEEGLRSVVVCEEGRTTPYERLGV
CCCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCHHHCCEEEEEECCCCCCCHHHHCC
ADEIIVVERFQDMLDEEVQERLRELNAIVVPHGSFVAYVGLDGIENEFCVPMFGNRRLLR
HHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCEEEEEECCCCCCCEEEEECCCCEEEE
WESERSLERKLLKRAGVKVPKVFDSPEDIDRPVIVKFPGARGGRGYFICSDPEEFEEKAE
ECHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCEEEECCCCCCCCEEEEECCHHHHHHHHH
RLIEDGVIDEEDLEQAHIEEYVVGTNFCVHYFRSVVEDTVEVLGMDRRYETNIDGLVRMP
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECC
AGDQLEAGLEPSYVISGNIPVVVRESLLVQLYEMGDRVVRASEDIEEPGFIGPFCLQTLC
CCCCHHCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHH
TEDLEFYVFELSARIDGGTNVTFLPYAYLKFGEIVTMGRRIAKEVREARDKGLLEGVVT
HHCHHEEEEEEEEEECCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11930014