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The map label for this gene is frhA [H]

Identifier: 20093975

GI number: 20093975

Start: 501838

End: 502950

Strand: Direct

Name: frhA [H]

Synonym: MK0537

Alternate gene names: 20093975

Gene position: 501838-502950 (Clockwise)

Preceding gene: 20093974

Following gene: 20093976

Centisome position: 29.61

GC content: 62.53

Gene sequence:

>1113_bases
ATGGTGGTGACCATGGAGATCGAGGCGATCCGTGTAGCAACGGGAGCTCCTGGCGAACTGTATATGGTAGCCGAGATCGC
GGAGGACAAGGTCGTGAACGCCGAAGTGTGCTCGGGCACGACACCGATCTCCTGGGAGACGCTCGTCCTCGAGAAGCCCG
TGGAGTTCGCCGTAGTGGCCGCCGAACGAGTATGCGCCAGCTGTGACGCGAGCCTAGGGCTCGCCGTGGTCGAGGCGGCC
GAGGCAGCTCTCAACGTCGAGATCCCGGACGAGGCGGAGCGAGCGCGCGAGATCCTGAACATGGCTAACGTCGTCAGGGC
GCACGCCACCGTGCTGGCGAGGACTGACTTCCTCGACGTGAAGAAGCCCGCGTACGACCTAGTGAGAGCTGCGAAGGACA
TCATGCACGCCGTCGGAGGGAAGCCCGACCACCCACCGGCCGTGACCGTAGGCGGTTTGGACCTCGAAAAGCTGCCCGTA
GACAGGGTTGAATCCGTAGCTAAGGACGCCGCCGAGGTGGCAGACAGGCTTGAGGACGAGGTCGGATCCGCGGTAGATCG
GATGAAGGAAGATATCGACGTGGAACCGGAGGAGATGCCGGCGGGACTGAAGGTTTCGGACACGTACAAGGGCGACATAG
ACCCAGACAAGGTCGAGACGCTAATGCCGGATGAATTCTACCGTATGAAGACCACCCTGGAGATAGCGAACAACGTCGTC
GCCAGGTATGACGGGGAGCCCGTCCTCGTCGGTCCTTACGCCCGAGTAAGGAGCGCGGAAAACCCGCTGGACCTCTACAC
CGCCAGGGCCGAGGAAGTAGCGAGGATGCTCGACGGTATCGCAAACGCCGTATCCAGGCTAGATCCGACCGGGAACTTCC
GAACGGACGTCGAGCTCGGCTCGGGCGAGGGTACGGCGGCTGTGGAGGCACCGGAGGGTACCCTGATCGTCTCGCTGAGA
CTACGGGCCGGGCGCGTGGACAAGGCCCTGATGTTAACTCCATGCAACTTCAAGGTTGCGGCGATCGGGGAGATGGTGCG
CGACCTGACGGTTGACAGGGCTGAGACAGTTCTACGCGCGATCGGCCTCTCCGGTAGATGTCTTACCCACTAG

Upstream 100 bases:

>100_bases
TCGTGGAGACGGACATGAAGCGTGCCGGAGAGATTCTCGCCGAGAAGATCGAGGAGAAGCGGAAACGGCTTGGTATCTAA
TTCTCACCCCCACTTCCGGG

Downstream 100 bases:

>100_bases
GGGGAAGCCCAAGATGAAGGTGGCTGTGCTGTCCCTTATGGCTTGTGGTACATGCGCCAGGAACCTGGAGGAGGTTTGCC
ACGAAGAGGGTCATGAGGTG

Product: coenzyme F420-reducing hydrogenase, alpha subunit

Products: NA

Alternate protein names: 8-hydroxy-5-deazaflavin-reducing hydrogenase subunit alpha; FRH [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVAAERVCASCDASLGLAVVEAA
EAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDVKKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPV
DRVESVAKDAAEVADRLEDEVGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVV
ARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELGSGEGTAAVEAPEGTLIVSLR
LRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRAIGLSGRCLTH

Sequences:

>Translated_370_residues
MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVAAERVCASCDASLGLAVVEAA
EAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDVKKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPV
DRVESVAKDAAEVADRLEDEVGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVV
ARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELGSGEGTAAVEAPEGTLIVSLR
LRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRAIGLSGRCLTH
>Mature_370_residues
MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVAAERVCASCDASLGLAVVEAA
EAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDVKKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPV
DRVESVAKDAAEVADRLEDEVGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVV
ARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELGSGEGTAAVEAPEGTLIVSLR
LRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRAIGLSGRCLTH

Specific function: Reduces the physiological low-potential two-electron acceptor coenzyme F420, and the artificial one-electron acceptor methylviologen [H]

COG id: COG3259

COG function: function code C; Coenzyme F420-reducing hydrogenase, alpha subunit

Gene ontology:

Cell location: Membrane-Bound [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the [NiFe]/[NiFeSe] hydrogenase large subunit family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017682
- InterPro:   IPR001501
- InterPro:   IPR018194 [H]

Pfam domain/function: PF00374 NiFeSe_Hases [H]

EC number: =1.12.98.1 [H]

Molecular weight: Translated: 39749; Mature: 39749

Theoretical pI: Translated: 4.30; Mature: 4.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVA
CEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEECCCCHHHHHH
AERVCASCDASLGLAVVEAAEAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDV
HHHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPVDRVESVAKDAAEVADRLEDE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVV
HHHHHHHHHHHCCCCHHHCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCHH
ARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELG
EEECCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
SGEGTAAVEAPEGTLIVSLRLRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRA
CCCCCEEEECCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGLSGRCLTH
HCCCCCCCCC
>Mature Secondary Structure
MVVTMEIEAIRVATGAPGELYMVAEIAEDKVVNAEVCSGTTPISWETLVLEKPVEFAVVA
CEEEEEEEEEEEECCCCCCEEEEEEECCCCCCCHHHCCCCCCCCEEEEEECCCCHHHHHH
AERVCASCDASLGLAVVEAAEAALNVEIPDEAERAREILNMANVVRAHATVLARTDFLDV
HHHHHHHCCCHHHHHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCC
KKPAYDLVRAAKDIMHAVGGKPDHPPAVTVGGLDLEKLPVDRVESVAKDAAEVADRLEDE
CCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHH
VGSAVDRMKEDIDVEPEEMPAGLKVSDTYKGDIDPDKVETLMPDEFYRMKTTLEIANNVV
HHHHHHHHHHHCCCCHHHCCCCCEECCCCCCCCCHHHHHHCCCCHHHHHHHHHHHHHCHH
ARYDGEPVLVGPYARVRSAENPLDLYTARAEEVARMLDGIANAVSRLDPTGNFRTDVELG
EEECCCEEEECCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEC
SGEGTAAVEAPEGTLIVSLRLRAGRVDKALMLTPCNFKVAAIGEMVRDLTVDRAETVLRA
CCCCCEEEECCCCEEEEEEEECCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
IGLSGRCLTH
HCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]