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The map label for this gene is thiC-1 [H]

Identifier: 197119825

GI number: 197119825

Start: 3976968

End: 3978281

Strand: Direct

Name: thiC-1 [H]

Synonym: Gbem_3463

Alternate gene names: 197119825

Gene position: 3976968-3978281 (Clockwise)

Preceding gene: 197119824

Following gene: 197119838

Centisome position: 86.17

GC content: 65.45

Gene sequence:

>1314_bases
ATGACTCAGCTCGAATACGCCCGCAAGGGGATCATTACCGACAAGATGAAGACCGCGGCCCTCGCCGAAGGGGTGGATCC
GGAATTCATCCGCGCCGGTATCGCCGCGGGCAACATCATCATCTGCCACAACAACAAGCACGCAAACGGGACCCCACTCG
CAGTGGGCAAGGGGCTCAGAACCAAGGTGAACGCCAACATCGGCTCCTCCGCCGACGACCTCAACTTCGAGAACGAGCTG
GAGAAGGTGCGCGTGGCCGTGGCCAGCGGCGCCGACGCCATCATGGACCTCTCGACCGGCGGACCGGTGGACGAGATCCG
CCGCGCCGTGATCGCCGAGACCTCCGCCTGCATCGGCACCGTGCCGCTGTACCAGGCGGCACTCGACGCGGTCAGAAAGC
ACAAGAAGGCGATCGTGGAGATGACGGTCGAGGACATCTTCCAGGGGGTGATCAAACACGCCGAGGACGGCGTCGACTTC
ATCACCGTGCACTGCGGAGTGACCCGTTCGACGGTCGAGCGCATGCGCAAGGAAGGGCGCATCATGGACGTGGTCTCCCG
CGGCGGCGCCTTCACCATCGAGTGGATGGCCCACAACAACGCGGAAAACCCGCTCTACGAGCACTTCGACCGCCTGCTGG
AAATCGTCAAGGCCTACGACATGACCCTGTCGCTGGGAGACGGCTTCCGCCCCGGCTGCCTGGCCGATGCGACCGACCGC
GCCCAGATCCACGAGCTGATCCTTTTGGGCGAACTCACCCAGCGCGCGCAGGAGTACGGCGTGCAGGTAATGATCGAGGG
GCCCGGCCACATGCCGCTGAACCAGATCGAGGCCAACATCCTCTTGCAGAAGAGGCTTTGCCACGGCGCTCCCTTCTACG
TGCTCGGGCCGCTGGTCACCGACATCGCACCGGGCTACGACCACATCACCTCGGCCATCGGCGGCACCATAGCCGCGGCC
GCCGGGGCCGACTTCCTCTGCTACGTGACACCTTCGGAGCACCTGAAGCTCCCGAGCGTCGAGGACGTGCGCGAGGGTGT
CATGGCGAGCCGCATCGCGGCACACGCAGCCGACATCGTCAAGGGGGTCAAGGGGGCCATGGAGAAGGACAACGAGATGG
CCCGCTGCCGCAAGAAGCTCGATTGGGAGGGGCAGTTCGCCCTCGCCATCGACCCGGTAAAGGCGCGCCGCCTGAGGGAG
GAGTCGGGGGTCGCCGACCACGGCGCCTGCACCATGTGCGGCGAGTTCTGCGCCTACAAGGTGATGGACGACGCCACCCA
GCGCGAAAAGGCCAACTCAGCCAACGCGGCCTAA

Upstream 100 bases:

>100_bases
CCATTTCATGGCGGCAAGAGACCAGGCCGAATAAACCAGGGGCTAGGGGCTGGGAATTTTCATCGACACGACAAGAAGCA
AGTTCAATAAAGGAGTAGAC

Downstream 100 bases:

>100_bases
ACCACCTGGTTACCGAGCCAGTCCCCATCCCTGCTGCCTAAAAAAAATAAAAGCCATGCCCGGCGCGCTGCCCGGCATGG
CTTTTTTGTTCCGAACTCCC

Product: thiamine biosynthesis protein ThiC

Products: @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine!; 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

Alternate protein names: Hydroxymethylpyrimidine phosphate synthase 2; HMP-P synthase 2; HMP-phosphate synthase 2; HMPP synthase 2; Thiamine biosynthesis protein thiC 2 [H]

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MTQLEYARKGIITDKMKTAALAEGVDPEFIRAGIAAGNIIICHNNKHANGTPLAVGKGLRTKVNANIGSSADDLNFENEL
EKVRVAVASGADAIMDLSTGGPVDEIRRAVIAETSACIGTVPLYQAALDAVRKHKKAIVEMTVEDIFQGVIKHAEDGVDF
ITVHCGVTRSTVERMRKEGRIMDVVSRGGAFTIEWMAHNNAENPLYEHFDRLLEIVKAYDMTLSLGDGFRPGCLADATDR
AQIHELILLGELTQRAQEYGVQVMIEGPGHMPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGTIAAA
AGADFLCYVTPSEHLKLPSVEDVREGVMASRIAAHAADIVKGVKGAMEKDNEMARCRKKLDWEGQFALAIDPVKARRLRE
ESGVADHGACTMCGEFCAYKVMDDATQREKANSANAA

Sequences:

>Translated_437_residues
MTQLEYARKGIITDKMKTAALAEGVDPEFIRAGIAAGNIIICHNNKHANGTPLAVGKGLRTKVNANIGSSADDLNFENEL
EKVRVAVASGADAIMDLSTGGPVDEIRRAVIAETSACIGTVPLYQAALDAVRKHKKAIVEMTVEDIFQGVIKHAEDGVDF
ITVHCGVTRSTVERMRKEGRIMDVVSRGGAFTIEWMAHNNAENPLYEHFDRLLEIVKAYDMTLSLGDGFRPGCLADATDR
AQIHELILLGELTQRAQEYGVQVMIEGPGHMPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGTIAAA
AGADFLCYVTPSEHLKLPSVEDVREGVMASRIAAHAADIVKGVKGAMEKDNEMARCRKKLDWEGQFALAIDPVKARRLRE
ESGVADHGACTMCGEFCAYKVMDDATQREKANSANAA
>Mature_436_residues
TQLEYARKGIITDKMKTAALAEGVDPEFIRAGIAAGNIIICHNNKHANGTPLAVGKGLRTKVNANIGSSADDLNFENELE
KVRVAVASGADAIMDLSTGGPVDEIRRAVIAETSACIGTVPLYQAALDAVRKHKKAIVEMTVEDIFQGVIKHAEDGVDFI
TVHCGVTRSTVERMRKEGRIMDVVSRGGAFTIEWMAHNNAENPLYEHFDRLLEIVKAYDMTLSLGDGFRPGCLADATDRA
QIHELILLGELTQRAQEYGVQVMIEGPGHMPLNQIEANILLQKRLCHGAPFYVLGPLVTDIAPGYDHITSAIGGTIAAAA
GADFLCYVTPSEHLKLPSVEDVREGVMASRIAAHAADIVKGVKGAMEKDNEMARCRKKLDWEGQFALAIDPVKARRLREE
SGVADHGACTMCGEFCAYKVMDDATQREKANSANAA

Specific function: Catalyzes the synthesis of the hydroxymethylpyrimidine phosphate (HMP-P) moiety of thiamine from aminoimidazole ribotide (AIR) in a radical S-adenosyl-L-methionine (SAM)-dependent reaction [H]

COG id: COG0422

COG function: function code H; Thiamine biosynthesis protein ThiC

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiC family [H]

Homologues:

Organism=Escherichia coli, GI1790427, Length=457, Percent_Identity=44.4201312910284, Blast_Score=373, Evalue=1e-104,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002817 [H]

Pfam domain/function: PF01964 ThiC [H]

EC number: NA

Molecular weight: Translated: 47221; Mature: 47089

Theoretical pI: Translated: 5.89; Mature: 5.89

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQLEYARKGIITDKMKTAALAEGVDPEFIRAGIAAGNIIICHNNKHANGTPLAVGKGLR
CCCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCC
TKVNANIGSSADDLNFENELEKVRVAVASGADAIMDLSTGGPVDEIRRAVIAETSACIGT
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCC
VPLYQAALDAVRKHKKAIVEMTVEDIFQGVIKHAEDGVDFITVHCGVTRSTVERMRKEGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCC
IMDVVSRGGAFTIEWMAHNNAENPLYEHFDRLLEIVKAYDMTLSLGDGFRPGCLADATDR
EEEHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCH
AQIHELILLGELTQRAQEYGVQVMIEGPGHMPLNQIEANILLQKRLCHGAPFYVLGPLVT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITSAIGGTIAAAAGADFLCYVTPSEHLKLPSVEDVREGVMASRIAAHAADIV
HCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KGVKGAMEKDNEMARCRKKLDWEGQFALAIDPVKARRLREESGVADHGACTMCGEFCAYK
HHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VMDDATQREKANSANAA
HHHHHHHHHHCCCCCCC
>Mature Secondary Structure 
TQLEYARKGIITDKMKTAALAEGVDPEFIRAGIAAGNIIICHNNKHANGTPLAVGKGLR
CCHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHCCCCEEEEECCCCCCCCCEEECCCCC
TKVNANIGSSADDLNFENELEKVRVAVASGADAIMDLSTGGPVDEIRRAVIAETSACIGT
EEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCCCCHHHHHHHHHHHHHHHHCC
VPLYQAALDAVRKHKKAIVEMTVEDIFQGVIKHAEDGVDFITVHCGVTRSTVERMRKEGR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHCCC
IMDVVSRGGAFTIEWMAHNNAENPLYEHFDRLLEIVKAYDMTLSLGDGFRPGCLADATDR
EEEHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCCCH
AQIHELILLGELTQRAQEYGVQVMIEGPGHMPLNQIEANILLQKRLCHGAPFYVLGPLVT
HHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
DIAPGYDHITSAIGGTIAAAAGADFLCYVTPSEHLKLPSVEDVREGVMASRIAAHAADIV
HCCCCHHHHHHHHHHHHHHHCCCCEEEEECCHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
KGVKGAMEKDNEMARCRKKLDWEGQFALAIDPVKARRLREESGVADHGACTMCGEFCAYK
HHHHHHHHCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHCCCCCCCHHHHHHHHHHHH
VMDDATQREKANSANAA
HHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ?; ?; 5'-phosphoribosyl-5-aminoimidazole [C]

Specific reaction: Synthesis of @AMIN06.txt*4-amino-2-methyl-5-hydroxymethylpyrimidine! 5'-phosphoribosyl-5-aminoimidazole = 4-amino-5-hydroxymethyl-2-methylpyrimidine-phosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA