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The map label for this gene is cysP [H]

Identifier: 197118108

GI number: 197118108

Start: 1987443

End: 1988432

Strand: Direct

Name: cysP [H]

Synonym: Gbem_1723

Alternate gene names: 197118108

Gene position: 1987443-1988432 (Clockwise)

Preceding gene: 197118107

Following gene: 197118109

Centisome position: 43.06

GC content: 59.9

Gene sequence:

>990_bases
ATGAAACAGCAGATTCGCACCGCATTACTCACACTGTTGGTGGCGGCCTTGCCGCTGACAGCCTTCGCCGCCCAGGAATT
CCTCAACGTCTCCTACGACCCGACCCGGGAGCTGTACCAGGACTTCAACAAGGCCTTTGCCAAGAAGTCCGGGCAAAAAG
CGACCTTCAAGCAGTCCCACGGCGGCTCCGGGAAGCAGGCGAGGGCAGTCATCGACGGGCTGGAAGCGGACGTCGTCACC
CTGGCTTTGGCCTACGACATCGACGAGATCAGCGACAAGGCGAAGCTGATCCCGGCCAACTGGCAGAAAAGACTCCCCAA
CAACAGCTCTCCCTACACTTCGACCATCGTCTTCCTGGTGCGCAAGGGAAACCCCAAAAAGATCAAGGACTGGAACGACC
TGGTTAAGCCGGGTGTCTCCGTGATCACCCCCAACCCGAAGACCTCCGGCGGTGCGCGCTGGAACTACCTCGCCGCCTGG
GGCTACGCGCTGAAAGGTGGCAATGAAACCAAGGCAAAGGCGTTTGTCGCCGAGCTCTTCAAGCATGTACCGGTGCTCGA
TTCCGGCGCACGCGGTTCCACCACGACCTTCGTGCAGCGCGGCCAGGGGGATGTGCTCCTCGCCTGGGAAAACGAGGCAT
TCCTCGCGGTCAACGAGCTGGGCAAAGACAAATTCGAGATCGTCGTTCCTTCGCTCAGCATCCTCGCCGAGCCGCCAGTG
ACCGTGGTCGACAAGGTGGTCGACAGGAAAGGGACCCGCAAACTCGCCGAGGAGTATCTGAAATACCTCTACTCCCCGGA
AGGTCAGGAAATCGCCGCGAGACATTACTACCGTCCGCGCGACAAGAAGGTCGCCGCCAAGTACGCCAAGGTCTTCCCAA
AAGTGAAGCTGGTGACCATTGACGACACCTTCGGAGGCTGGCGCAAGGCCCAGAAGACGCACTTTGCCGACGGCGGCGTT
TTCGATCAGATTTACTCCGGCGGAAAGTAG

Upstream 100 bases:

>100_bases
TTGCTCAGCAGGACAGTGGATATCCCGAATGACAAAGGTGCTTTTTTTTATCTCAATAGTCTATCAAGTCGGTGGATTAA
ACATTAAAAGGGGGAGCATC

Downstream 100 bases:

>100_bases
CCCGGAGACAACCCGGTTGATGCAGGTTCCTGGAGGGGGCATGGCGACGACACCCGAACAAGCTGGAAAGTGGAACAGCG
ATCTCGAGCAAAAGATCCGT

Product: sulfate ABC transporter periplasmic sulfate-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 329; Mature: 329

Protein sequence:

>329_residues
MKQQIRTALLTLLVAALPLTAFAAQEFLNVSYDPTRELYQDFNKAFAKKSGQKATFKQSHGGSGKQARAVIDGLEADVVT
LALAYDIDEISDKAKLIPANWQKRLPNNSSPYTSTIVFLVRKGNPKKIKDWNDLVKPGVSVITPNPKTSGGARWNYLAAW
GYALKGGNETKAKAFVAELFKHVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGKDKFEIVVPSLSILAEPPV
TVVDKVVDRKGTRKLAEEYLKYLYSPEGQEIAARHYYRPRDKKVAAKYAKVFPKVKLVTIDDTFGGWRKAQKTHFADGGV
FDQIYSGGK

Sequences:

>Translated_329_residues
MKQQIRTALLTLLVAALPLTAFAAQEFLNVSYDPTRELYQDFNKAFAKKSGQKATFKQSHGGSGKQARAVIDGLEADVVT
LALAYDIDEISDKAKLIPANWQKRLPNNSSPYTSTIVFLVRKGNPKKIKDWNDLVKPGVSVITPNPKTSGGARWNYLAAW
GYALKGGNETKAKAFVAELFKHVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGKDKFEIVVPSLSILAEPPV
TVVDKVVDRKGTRKLAEEYLKYLYSPEGQEIAARHYYRPRDKKVAAKYAKVFPKVKLVTIDDTFGGWRKAQKTHFADGGV
FDQIYSGGK
>Mature_329_residues
MKQQIRTALLTLLVAALPLTAFAAQEFLNVSYDPTRELYQDFNKAFAKKSGQKATFKQSHGGSGKQARAVIDGLEADVVT
LALAYDIDEISDKAKLIPANWQKRLPNNSSPYTSTIVFLVRKGNPKKIKDWNDLVKPGVSVITPNPKTSGGARWNYLAAW
GYALKGGNETKAKAFVAELFKHVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGKDKFEIVVPSLSILAEPPV
TVVDKVVDRKGTRKLAEEYLKYLYSPEGQEIAARHYYRPRDKKVAAKYAKVFPKVKLVTIDDTFGGWRKAQKTHFADGGV
FDQIYSGGK

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=321, Percent_Identity=70.404984423676, Blast_Score=458, Evalue=1e-130,
Organism=Escherichia coli, GI1788765, Length=313, Percent_Identity=49.8402555910543, Blast_Score=306, Evalue=1e-84,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36357; Mature: 36357

Theoretical pI: Translated: 10.18; Mature: 10.18

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQQIRTALLTLLVAALPLTAFAAQEFLNVSYDPTRELYQDFNKAFAKKSGQKATFKQSH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCC
GGSGKQARAVIDGLEADVVTLALAYDIDEISDKAKLIPANWQKRLPNNSSPYTSTIVFLV
CCCCHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCEECCCCHHHHCCCCCCCCEEEEEEEE
RKGNPKKIKDWNDLVKPGVSVITPNPKTSGGARWNYLAAWGYALKGGNETKAKAFVAELF
ECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEHHEEEHHEECCCCCHHHHHHHHHHH
KHVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGKDKFEIVVPSLSILAEPPV
HHCCCCCCCCCCCCHHHEECCCCCEEEEECCCEEEEHHHCCCCEEEEEECCHHHHCCCCH
TVVDKVVDRKGTRKLAEEYLKYLYSPEGQEIAARHYYRPRDKKVAAKYAKVFPKVKLVTI
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEE
DDTFGGWRKAQKTHFADGGVFDQIYSGGK
ECCCCCHHHHHHCCCCCCCHHHHHHCCCC
>Mature Secondary Structure
MKQQIRTALLTLLVAALPLTAFAAQEFLNVSYDPTRELYQDFNKAFAKKSGQKATFKQSH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCC
GGSGKQARAVIDGLEADVVTLALAYDIDEISDKAKLIPANWQKRLPNNSSPYTSTIVFLV
CCCCHHHHHHHHCCCHHHHHHHHHHCHHHHCCCCEECCCCHHHHCCCCCCCCEEEEEEEE
RKGNPKKIKDWNDLVKPGVSVITPNPKTSGGARWNYLAAWGYALKGGNETKAKAFVAELF
ECCCCCCCCCHHHHHHCCCEEECCCCCCCCCCCEEHHEEEHHEECCCCCHHHHHHHHHHH
KHVPVLDSGARGSTTTFVQRGQGDVLLAWENEAFLAVNELGKDKFEIVVPSLSILAEPPV
HHCCCCCCCCCCCCHHHEECCCCCEEEEECCCEEEEHHHCCCCEEEEEECCHHHHCCCCH
TVVDKVVDRKGTRKLAEEYLKYLYSPEGQEIAARHYYRPRDKKVAAKYAKVFPKVKLVTI
HHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHCCCCCHHHHHHHHHHHCCCEEEEEE
DDTFGGWRKAQKTHFADGGVFDQIYSGGK
ECCCCCHHHHHHCCCCCCCHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]