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The map label for this gene is rfaQ [H]

Identifier: 197117252

GI number: 197117252

Start: 1015859

End: 1016878

Strand: Direct

Name: rfaQ [H]

Synonym: Gbem_0860

Alternate gene names: 197117252

Gene position: 1015859-1016878 (Clockwise)

Preceding gene: 197117251

Following gene: 197117253

Centisome position: 22.01

GC content: 60.98

Gene sequence:

>1020_bases
ATGAACATATTGATTATCAAGCCGGGCGCCATTGGCGACCTCCTGCTTATCACGCCTGTGGTGCGGGCACTGCGTGGGAT
CTATCCGTCGGCAAGGGTTACCCTCGTGGTCAGCTCCAGGGGGACCGCCTCGCTTTTCAGCCACAATCCGTTGGTGAACG
ACGTCATCGTTTTCGACAGAAAGGGGGAGCACAGCAGTTGGGGCAGTCTGTTTCAGCTCTGGCGGCGCATCAGGGAAAAG
AAGTTCGATCTGGTGCTGAACTTCCAGCGCAGCAACCTAAAGGGGTGGCTCCTGGCCAGTGCGGCTCTTCCCAGCCGAAT
CCTCGTCTACCATAAGGCGAAAAGAAGGATCGTGCACGCCGTGGTGAACCATCTGGAGACGTTGAAGCCCCTGGGGATAG
AACCGGCGAACTGCGAGATCTCGCTTCAAATCGGAGTAGGAAAGGAAGACGAAGCCTTTGCTGCCGATCTCCTCCGGCCG
CTGTGGCTGGAGGGGAGGGACGTGGTGGCAGTGAACCCGGGGGCGAGCCACCCGGTGAACCGGTGGGGCGTGGAGCATTT
TGCCGGGCTGTGCGACCTTCTCTCTGAGCGGCTTGGGGCGAAGGTCATTATTGTCGGCGGTGCAGACGATACTGTCCTCG
CGGACCAGATTGTGGCTAAGGCGACGTCCTCCCCTGTAGTATTGACCGGAAAAACCTCTCTGCTGCAACTGGGAGCCATT
TTGCAGAAATCCGCATTGCTGGTCACCGGCGATACGGGGCCGATGCACATCGCTACGGCGGTGGGTACTCGAGTGGTGGC
GCTTTTCGGCGCGGCCGATCCCGAGAGGACCGGCCCGGTGGGGGAGGGGCACAGGGTCATGCAGGCGCGCGAGGTGGCCT
GCGTTCCCTGCCGCAGCCGCAAGTGCGCCAATGAAAACTACCTGGAGTGCATGAACCGGATCACGCCGGAGCAAGTGTTC
GAGGCGGTCAAGGAGATGCTGCTGCAGCCGTGCCGCACCAGCGGAAGCGTTTGCAAGTGA

Upstream 100 bases:

>100_bases
GCAGATCCGCTATGGCAACGCTGGCAGGTTGACTTGCATTTTGGTGCCAACAGGATGGCTACCATGGGGCAGCTGCCCCT
GCTTCAACAGAAGAGGACGG

Downstream 100 bases:

>100_bases
CGATGATGTTTTATTCCCTTCAATATGGCAATTTCTGGAGGTCAGATGACAAAGATACTTGTTACCGGCGCAGCGGGTTT
CATAGGCTCGCACCTTTCCC

Product: lipopolysaccharide heptosyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MNILIIKPGAIGDLLLITPVVRALRGIYPSARVTLVVSSRGTASLFSHNPLVNDVIVFDRKGEHSSWGSLFQLWRRIREK
KFDLVLNFQRSNLKGWLLASAALPSRILVYHKAKRRIVHAVVNHLETLKPLGIEPANCEISLQIGVGKEDEAFAADLLRP
LWLEGRDVVAVNPGASHPVNRWGVEHFAGLCDLLSERLGAKVIIVGGADDTVLADQIVAKATSSPVVLTGKTSLLQLGAI
LQKSALLVTGDTGPMHIATAVGTRVVALFGAADPERTGPVGEGHRVMQAREVACVPCRSRKCANENYLECMNRITPEQVF
EAVKEMLLQPCRTSGSVCK

Sequences:

>Translated_339_residues
MNILIIKPGAIGDLLLITPVVRALRGIYPSARVTLVVSSRGTASLFSHNPLVNDVIVFDRKGEHSSWGSLFQLWRRIREK
KFDLVLNFQRSNLKGWLLASAALPSRILVYHKAKRRIVHAVVNHLETLKPLGIEPANCEISLQIGVGKEDEAFAADLLRP
LWLEGRDVVAVNPGASHPVNRWGVEHFAGLCDLLSERLGAKVIIVGGADDTVLADQIVAKATSSPVVLTGKTSLLQLGAI
LQKSALLVTGDTGPMHIATAVGTRVVALFGAADPERTGPVGEGHRVMQAREVACVPCRSRKCANENYLECMNRITPEQVF
EAVKEMLLQPCRTSGSVCK
>Mature_339_residues
MNILIIKPGAIGDLLLITPVVRALRGIYPSARVTLVVSSRGTASLFSHNPLVNDVIVFDRKGEHSSWGSLFQLWRRIREK
KFDLVLNFQRSNLKGWLLASAALPSRILVYHKAKRRIVHAVVNHLETLKPLGIEPANCEISLQIGVGKEDEAFAADLLRP
LWLEGRDVVAVNPGASHPVNRWGVEHFAGLCDLLSERLGAKVIIVGGADDTVLADQIVAKATSSPVVLTGKTSLLQLGAI
LQKSALLVTGDTGPMHIATAVGTRVVALFGAADPERTGPVGEGHRVMQAREVACVPCRSRKCANENYLECMNRITPEQVF
EAVKEMLLQPCRTSGSVCK

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=345, Percent_Identity=27.2463768115942, Blast_Score=108, Evalue=5e-25,
Organism=Escherichia coli, GI1790050, Length=356, Percent_Identity=26.9662921348315, Blast_Score=96, Evalue=3e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.4.1.-

Molecular weight: Translated: 36790; Mature: 36790

Theoretical pI: Translated: 9.38; Mature: 9.38

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNILIIKPGAIGDLLLITPVVRALRGIYPSARVTLVVSSRGTASLFSHNPLVNDVIVFDR
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHCCCCCCCEEEEEEC
KGEHSSWGSLFQLWRRIREKKFDLVLNFQRSNLKGWLLASAALPSRILVYHKAKRRIVHA
CCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEHHCCCHHHHHHHHHHHHHHHH
VVNHLETLKPLGIEPANCEISLQIGVGKEDEAFAADLLRPLWLEGRDVVAVNPGASHPVN
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCH
RWGVEHFAGLCDLLSERLGAKVIIVGGADDTVLADQIVAKATSSPVVLTGKTSLLQLGAI
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
LQKSALLVTGDTGPMHIATAVGTRVVALFGAADPERTGPVGEGHRVMQAREVACVPCRSR
HHCCEEEEECCCCCEEHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHEECCCCCC
KCANENYLECMNRITPEQVFEAVKEMLLQPCRTSGSVCK
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MNILIIKPGAIGDLLLITPVVRALRGIYPSARVTLVVSSRGTASLFSHNPLVNDVIVFDR
CEEEEECCCCCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCEEHHCCCCCCCEEEEEEC
KGEHSSWGSLFQLWRRIREKKFDLVLNFQRSNLKGWLLASAALPSRILVYHKAKRRIVHA
CCCCCCHHHHHHHHHHHHHCCEEEEEEEECCCCCEEEEEHHCCCHHHHHHHHHHHHHHHH
VVNHLETLKPLGIEPANCEISLQIGVGKEDEAFAADLLRPLWLEGRDVVAVNPGASHPVN
HHHHHHHHCCCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEECCCCCCCCH
RWGVEHFAGLCDLLSERLGAKVIIVGGADDTVLADQIVAKATSSPVVLTGKTSLLQLGAI
HHHHHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCEEEECCHHHHHHHHH
LQKSALLVTGDTGPMHIATAVGTRVVALFGAADPERTGPVGEGHRVMQAREVACVPCRSR
HHCCEEEEECCCCCEEHHHHHHHHHHHEECCCCCCCCCCCCCCHHHHHHHHHEECCCCCC
KCANENYLECMNRITPEQVFEAVKEMLLQPCRTSGSVCK
CCCCCHHHHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA