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The map label for this gene is dagK [H]

Identifier: 197117104

GI number: 197117104

Start: 841471

End: 842340

Strand: Direct

Name: dagK [H]

Synonym: Gbem_0709

Alternate gene names: 197117104

Gene position: 841471-842340 (Clockwise)

Preceding gene: 197117103

Following gene: 197117105

Centisome position: 18.23

GC content: 68.28

Gene sequence:

>870_bases
TTGACCAGACGCTGCTTTCTCATCGTGAACCCCACCTCGGGAAGCTATTCCCAGAGAACCGTGGATCAGATCATGGCCGG
CCTCAGGGAGAGGGGGCTCGCACCCGAGCTGATGCCGACGGGAAGCGCCGCCGACCCGGCGCTCTTCGCTGCACGCCTTT
GCGCCCAACACTCCGATCCGCTGGTCATAGTCGCCGGCGGCGACGGCACGGTGAACGGCGTCCTGAACGGCCTGGTCCCC
GGTAGGGCGACCCTCGGGGTGGTCCCCTTGGGGACCGCCAACGTCCTGGCGCGGGAGTTGGGTATCTCTTCCGTCGCCGA
CGCCCTGGACCGCCTGGCCAGAGGGACGACCCGACCCATCTCCGTGGGCGAGATCGAGCGGGACGGGGAGAAGAGGCGCT
TCCTCTTGATGGCGGGCGCCGGGATGGACGGTGCGGTGGTGCGCGGGGTGCGCCTCTCCGAGAAGCGGGTCATAGGAAAA
GGGGCCTACCTCCTCTCGGCGCTCAGGGTGCTCCTTTCTTGGGACCGGTCGGAACTCCGGGTTACAGGCGGTGGGCGGAG
CCTCTCCTGCCACGGCGTGATCGTCTGCAACGCCGCCAAGTACGGCGGCAATTTCGTGCTCGCCCCCGAGGCGGACCTCT
TCTCCCCGGGGTTCCAGGTGCTCTGCATCGAGGGGGGGCGGCTCGCCTACCTGAAGCTCGGCCTCATGCTGCTATCCGGC
AGGGGGGCGCCCCCCAGCTGCGTGACCTCGTTCGCCGCGACGGAACTGGAGCTCTCCGGCGACAAGGCGGTGCAACTGGA
CGGCGACTATTTCTGCTGCACCCCGTTGCACATCAGGACGCTCCCGGAGCTGGTCCGCCTGGTGGTCTGA

Upstream 100 bases:

>100_bases
TATACAGATTGGAAAGAAAAAGGTGCCTCACGAGTCCCCGCCCCCGGAGGGGGAGGGTTAGGGAGGGGGCCTTTCATTTC
ATCCGGACGAGGAGTCCCGC

Downstream 100 bases:

>100_bases
GGCGCCGCTGGCGGCCAAATTCCCCTGTTGACAAAGTAACGGCAATATCGGATTAATGTGGCATTCGCCCCCTGCCGGGG
GGCGGTGCTTCCCGGCGGCT

Product: sphingosine/diacylglycerol kinase-like protein

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 289; Mature: 288

Protein sequence:

>289_residues
MTRRCFLIVNPTSGSYSQRTVDQIMAGLRERGLAPELMPTGSAADPALFAARLCAQHSDPLVIVAGGDGTVNGVLNGLVP
GRATLGVVPLGTANVLARELGISSVADALDRLARGTTRPISVGEIERDGEKRRFLLMAGAGMDGAVVRGVRLSEKRVIGK
GAYLLSALRVLLSWDRSELRVTGGGRSLSCHGVIVCNAAKYGGNFVLAPEADLFSPGFQVLCIEGGRLAYLKLGLMLLSG
RGAPPSCVTSFAATELELSGDKAVQLDGDYFCCTPLHIRTLPELVRLVV

Sequences:

>Translated_289_residues
MTRRCFLIVNPTSGSYSQRTVDQIMAGLRERGLAPELMPTGSAADPALFAARLCAQHSDPLVIVAGGDGTVNGVLNGLVP
GRATLGVVPLGTANVLARELGISSVADALDRLARGTTRPISVGEIERDGEKRRFLLMAGAGMDGAVVRGVRLSEKRVIGK
GAYLLSALRVLLSWDRSELRVTGGGRSLSCHGVIVCNAAKYGGNFVLAPEADLFSPGFQVLCIEGGRLAYLKLGLMLLSG
RGAPPSCVTSFAATELELSGDKAVQLDGDYFCCTPLHIRTLPELVRLVV
>Mature_288_residues
TRRCFLIVNPTSGSYSQRTVDQIMAGLRERGLAPELMPTGSAADPALFAARLCAQHSDPLVIVAGGDGTVNGVLNGLVPG
RATLGVVPLGTANVLARELGISSVADALDRLARGTTRPISVGEIERDGEKRRFLLMAGAGMDGAVVRGVRLSEKRVIGKG
AYLLSALRVLLSWDRSELRVTGGGRSLSCHGVIVCNAAKYGGNFVLAPEADLFSPGFQVLCIEGGRLAYLKLGLMLLSGR
GAPPSCVTSFAATELELSGDKAVQLDGDYFCCTPLHIRTLPELVRLVV

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=270, Percent_Identity=26.6666666666667, Blast_Score=61, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 30388; Mature: 30257

Theoretical pI: Translated: 8.58; Mature: 8.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRRCFLIVNPTSGSYSQRTVDQIMAGLRERGLAPELMPTGSAADPALFAARLCAQHSDP
CCCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
LVIVAGGDGTVNGVLNGLVPGRATLGVVPLGTANVLARELGISSVADALDRLARGTTRPI
EEEEECCCCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
SVGEIERDGEKRRFLLMAGAGMDGAVVRGVRLSEKRVIGKGAYLLSALRVLLSWDRSELR
CCCCCCCCCCCEEEEEEECCCCCCHHEECEEECCCEEECCHHHHHHHHHHHHHCCCCEEE
VTGGGRSLSCHGVIVCNAAKYGGNFVLAPEADLFSPGFQVLCIEGGRLAYLKLGLMLLSG
EECCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCEEEHEEEEHEEEC
RGAPPSCVTSFAATELELSGDKAVQLDGDYFCCTPLHIRTLPELVRLVV
CCCCHHHHHHHHHEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHHC
>Mature Secondary Structure 
TRRCFLIVNPTSGSYSQRTVDQIMAGLRERGLAPELMPTGSAADPALFAARLCAQHSDP
CCEEEEEECCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHCCCCC
LVIVAGGDGTVNGVLNGLVPGRATLGVVPLGTANVLARELGISSVADALDRLARGTTRPI
EEEEECCCCCHHHHHHCCCCCCCEEEEEECCHHHHHHHHHCHHHHHHHHHHHHCCCCCCC
SVGEIERDGEKRRFLLMAGAGMDGAVVRGVRLSEKRVIGKGAYLLSALRVLLSWDRSELR
CCCCCCCCCCCEEEEEEECCCCCCHHEECEEECCCEEECCHHHHHHHHHHHHHCCCCEEE
VTGGGRSLSCHGVIVCNAAKYGGNFVLAPEADLFSPGFQVLCIEGGRLAYLKLGLMLLSG
EECCCCEEEEEEEEEEECCCCCCEEEECCCCCCCCCCCEEEEEECCCEEEHEEEEHEEEC
RGAPPSCVTSFAATELELSGDKAVQLDGDYFCCTPLHIRTLPELVRLVV
CCCCHHHHHHHHHEEEEECCCEEEEECCCEEEECCHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]