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The map label for this gene is pleD [H]

Identifier: 197117105

GI number: 197117105

Start: 842517

End: 843239

Strand: Direct

Name: pleD [H]

Synonym: Gbem_0710

Alternate gene names: 197117105

Gene position: 842517-843239 (Clockwise)

Preceding gene: 197117104

Following gene: 197117109

Centisome position: 18.26

GC content: 63.07

Gene sequence:

>723_bases
ATGGCTTATCCAAAGATAATGCTGGTGGACGACACCCGGCTGATACTGGAGCTTGAGAAGAGTTTCCTCAAGTTGTCGCA
CGTGGACGTGGTAACCGCCGGCAACGGCGCGGAGGCGCTGGAGCTGATCCGTAAGGATCCCCCCGATCTGATCTTCATGG
ATATGCATATGCCTGTCATGGACGGCATCGCCTGCTGCACCGAACTCAAGGCCGACCCGTTTCTTTGCGGCATCCCGGTG
GTGATGCTGACGACGGCGGGGAAGGAGGAAGACCGCGAGCTGGCCAAAGCGGCCGGCTGCGACAACTTCCTGACCAAACC
GGTCGACCGCCGCTTATTCCTGGACCTGGCCCGCAAGTACACCAACGCCGTGGACCGGCGCGAGCAGCGTCTCCCCTGCC
GTTTGCCGGCTCTGTTCCTGGTGGGCAAGGCCCCCGCCGCCGGCCTTGTCCTCGACATAGGCGACGGCGGCGTCTTCGTG
GCAACCAAGGAGCGGCTGCTCTTGCACCAGAGGCTGCGCCTTGCGCTCTATCTGGAGGGGGAACGTCCGGTACTGCTGGA
ACTAAAAGGGTGGGCGGCCTGGCTGAACGAGGAGGGAAAACGCGTGCACAGTTCGCTGCCAGCCGGCTTCGGGATGGAGT
TCCTGGAGTTGGGCGAAGAGGAGAGTGCGGCGCTGAAATCGTACCTCGCCTCGCTCAGGCCGGAAGAGCCGTCGGCATCC
TAG

Upstream 100 bases:

>100_bases
GGGGGGCGGTGCTTCCCGGCGGCTTGGCTTGCAGGCGGCTCTTCAGCGCGGTTTCGGGCAGGACTTTTAGCTGTAGACAT
GCATCACAGGGGGACTTGGC

Downstream 100 bases:

>100_bases
CGGCAAAGACGCAAGGTTGCCTGAAACCAGAACCAGGTGAAAAGGGCCGCGGCGCAGTTGCCGGCCCAGTTGTCGATGAG
CCGCCCCAAAAGGCGGTCGT

Product: PilZ domain-containing response regulator

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 240; Mature: 239

Protein sequence:

>240_residues
MAYPKIMLVDDTRLILELEKSFLKLSHVDVVTAGNGAEALELIRKDPPDLIFMDMHMPVMDGIACCTELKADPFLCGIPV
VMLTTAGKEEDRELAKAAGCDNFLTKPVDRRLFLDLARKYTNAVDRREQRLPCRLPALFLVGKAPAAGLVLDIGDGGVFV
ATKERLLLHQRLRLALYLEGERPVLLELKGWAAWLNEEGKRVHSSLPAGFGMEFLELGEEESAALKSYLASLRPEEPSAS

Sequences:

>Translated_240_residues
MAYPKIMLVDDTRLILELEKSFLKLSHVDVVTAGNGAEALELIRKDPPDLIFMDMHMPVMDGIACCTELKADPFLCGIPV
VMLTTAGKEEDRELAKAAGCDNFLTKPVDRRLFLDLARKYTNAVDRREQRLPCRLPALFLVGKAPAAGLVLDIGDGGVFV
ATKERLLLHQRLRLALYLEGERPVLLELKGWAAWLNEEGKRVHSSLPAGFGMEFLELGEEESAALKSYLASLRPEEPSAS
>Mature_239_residues
AYPKIMLVDDTRLILELEKSFLKLSHVDVVTAGNGAEALELIRKDPPDLIFMDMHMPVMDGIACCTELKADPFLCGIPVV
MLTTAGKEEDRELAKAAGCDNFLTKPVDRRLFLDLARKYTNAVDRREQRLPCRLPALFLVGKAPAAGLVLDIGDGGVFVA
TKERLLLHQRLRLALYLEGERPVLLELKGWAAWLNEEGKRVHSSLPAGFGMEFLELGEEESAALKSYLASLRPEEPSAS

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1788394, Length=108, Percent_Identity=31.4814814814815, Blast_Score=65, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 26559; Mature: 26428

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYPKIMLVDDTRLILELEKSFLKLSHVDVVTAGNGAEALELIRKDPPDLIFMDMHMPVM
CCCCCEEEECCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHH
DGIACCTELKADPFLCGIPVVMLTTAGKEEDRELAKAAGCDNFLTKPVDRRLFLDLARKY
HHHHHHHHCCCCCCEECCCEEEEECCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
TNAVDRREQRLPCRLPALFLVGKAPAAGLVLDIGDGGVFVATKERLLLHQRLRLALYLEG
HHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEHHHHHHHHHHEEEEEECC
ERPVLLELKGWAAWLNEEGKRVHSSLPAGFGMEFLELGEEESAALKSYLASLRPEEPSAS
CCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYPKIMLVDDTRLILELEKSFLKLSHVDVVTAGNGAEALELIRKDPPDLIFMDMHMPVM
CCCCEEEECCCHHHHHHHHHHHHHHHEEEEECCCCHHHHHHHHCCCCCEEEEECCCCHH
DGIACCTELKADPFLCGIPVVMLTTAGKEEDRELAKAAGCDNFLTKPVDRRLFLDLARKY
HHHHHHHHCCCCCCEECCCEEEEECCCCHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHH
TNAVDRREQRLPCRLPALFLVGKAPAAGLVLDIGDGGVFVATKERLLLHQRLRLALYLEG
HHHHHHHHHCCCCCCCCEEEEECCCCCCEEEEECCCCEEEEEHHHHHHHHHHEEEEEECC
ERPVLLELKGWAAWLNEEGKRVHSSLPAGFGMEFLELGEEESAALKSYLASLRPEEPSAS
CCCEEEEECHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA