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The map label for this gene is lipB

Identifier: 197117102

GI number: 197117102

Start: 838190

End: 838804

Strand: Direct

Name: lipB

Synonym: Gbem_0707

Alternate gene names: 197117102

Gene position: 838190-838804 (Clockwise)

Preceding gene: 308535184

Following gene: 197117103

Centisome position: 18.16

GC content: 65.69

Gene sequence:

>615_bases
ATGATCTTCAAGGACGTCGGAGTCATCGGCTATGCCGAGGCGCTCCGGATACAGGAACAACTGGTGGCGGAGGTGCAACA
GGGGGGCGAGGAGGCCCTCTTGCTGCTTGAGCACCTCCCCGTCTACACCATCGGCGCGGGGGGGGACCGGGGCAACGTCC
TCGATCCGGAATTGGAGCCGGTCCGGGTGAACCGCGGCGGGGACGTCACCTACCACGGCCCGGGCCAGTTGGTCTGCTAC
CCGATCCTCGACCTGTCCCGGCGCGGGCGCGACCTGCACCGCTACCTTCGTTTCCTGGAGCGCTTCCTGGTCGAGCTTTG
CGCCGGCCTCGGGGTCGGCTGCCATACCGTCCCCGGCCGCACCGGCGTCTGGACCGGCAACGGCAAGCTCGCTTCCATAG
GGGTAGGGGTACGGCGCTGGGTCTCCATGCACGGCTTCGCGCTGAACGTCTCACCGGACACCGCCCCCTTCTCCAGGATC
AACCCCTGCGGCATGCCGGGCTGCAGCATCACCTCCTTGAGCCTGGAACTGGGGGAGGAGCTATTCGTCGATGAGTTGAA
AGAAATGGTCGCCATCCGTTTCCAACCGCTTTTGAACCTGCACCTGCCACGATAG

Upstream 100 bases:

>100_bases
ATGCATGCCCACCCGACCCTCTCCGAGGCGATGAAGGAAGCGGCGCTGGACGTGGACAAGAGGGCGATACACGGCTGATT
CGGTTTTTTCGGTGGAGCAT

Downstream 100 bases:

>100_bases
CCCAATGAAAGGTGGGCGAGGAGGCTCCTCATGTCCGAAACCGACTGCCGCGTCCCTGTAGAGAAGCTTCGCTGGGTGTG
CGATCCCGCCCTCTTCAATT

Product: octanoyl-(acyl carrier protein)--protein octanoyltransferase

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MIFKDVGVIGYAEALRIQEQLVAEVQQGGEEALLLLEHLPVYTIGAGGDRGNVLDPELEPVRVNRGGDVTYHGPGQLVCY
PILDLSRRGRDLHRYLRFLERFLVELCAGLGVGCHTVPGRTGVWTGNGKLASIGVGVRRWVSMHGFALNVSPDTAPFSRI
NPCGMPGCSITSLSLELGEELFVDELKEMVAIRFQPLLNLHLPR

Sequences:

>Translated_204_residues
MIFKDVGVIGYAEALRIQEQLVAEVQQGGEEALLLLEHLPVYTIGAGGDRGNVLDPELEPVRVNRGGDVTYHGPGQLVCY
PILDLSRRGRDLHRYLRFLERFLVELCAGLGVGCHTVPGRTGVWTGNGKLASIGVGVRRWVSMHGFALNVSPDTAPFSRI
NPCGMPGCSITSLSLELGEELFVDELKEMVAIRFQPLLNLHLPR
>Mature_204_residues
MIFKDVGVIGYAEALRIQEQLVAEVQQGGEEALLLLEHLPVYTIGAGGDRGNVLDPELEPVRVNRGGDVTYHGPGQLVCY
PILDLSRRGRDLHRYLRFLERFLVELCAGLGVGCHTVPGRTGVWTGNGKLASIGVGVRRWVSMHGFALNVSPDTAPFSRI
NPCGMPGCSITSLSLELGEELFVDELKEMVAIRFQPLLNLHLPR

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG0321

COG function: function code H; Lipoate-protein ligase B

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the lipB family

Homologues:

Organism=Homo sapiens, GI221554520, Length=214, Percent_Identity=35.5140186915888, Blast_Score=95, Evalue=4e-20,
Organism=Escherichia coli, GI87081767, Length=145, Percent_Identity=41.3793103448276, Blast_Score=117, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6323268, Length=168, Percent_Identity=30.952380952381, Blast_Score=73, Evalue=3e-14,
Organism=Drosophila melanogaster, GI21355483, Length=208, Percent_Identity=34.6153846153846, Blast_Score=117, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LIPB_GEOBB (B5EDZ5)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137529.1
- ProteinModelPortal:   B5EDZ5
- SMR:   B5EDZ5
- GeneID:   6783540
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0707
- HOGENOM:   HBG716160
- OMA:   DLKRRRP
- ProtClustDB:   CLSK828834
- GO:   GO:0005737
- HAMAP:   MF_00013
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605
- PIRSF:   PIRSF016262
- TIGRFAMs:   TIGR00214

Pfam domain/function: PF03099 BPL_LipA_LipB

EC number: =2.3.1.181

Molecular weight: Translated: 22341; Mature: 22341

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS01313 LIPB

Important sites: ACT_SITE 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFKDVGVIGYAEALRIQEQLVAEVQQGGEEALLLLEHLPVYTIGAGGDRGNVLDPELEP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCE
VRVNRGGDVTYHGPGQLVCYPILDLSRRGRDLHRYLRFLERFLVELCAGLGVGCHTVPGR
EEECCCCCEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
TGVWTGNGKLASIGVGVRRWVSMHGFALNVSPDTAPFSRINPCGMPGCSITSLSLELGEE
CEEEECCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHH
LFVDELKEMVAIRFQPLLNLHLPR
HHHHHHHHHHHHHHHHHHEECCCC
>Mature Secondary Structure
MIFKDVGVIGYAEALRIQEQLVAEVQQGGEEALLLLEHLPVYTIGAGGDRGNVLDPELEP
CCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCE
VRVNRGGDVTYHGPGQLVCYPILDLSRRGRDLHRYLRFLERFLVELCAGLGVGCHTVPGR
EEECCCCCEEECCCCCEEEEECHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCEECCCC
TGVWTGNGKLASIGVGVRRWVSMHGFALNVSPDTAPFSRINPCGMPGCSITSLSLELGEE
CEEEECCCEEEEEHHHHHHHHHHCCEEEEECCCCCCCCCCCCCCCCCCCEEEHHHHHHHH
LFVDELKEMVAIRFQPLLNLHLPR
HHHHHHHHHHHHHHHHHHEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA