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The map label for this gene is thiG

Identifier: 197117050

GI number: 197117050

Start: 775367

End: 776149

Strand: Reverse

Name: thiG

Synonym: Gbem_0653

Alternate gene names: 197117050

Gene position: 776149-775367 (Counterclockwise)

Preceding gene: 197117051

Following gene: 197117049

Centisome position: 16.82

GC content: 64.37

Gene sequence:

>783_bases
ATGACCCAGACAAACGACAAGCTCGTTATTGCAGGACGCGAATTCGACTCCCGCCTCATGGTCGGCACCGGAAAATATGC
CGACTTCCAGCAGATGGTGCGCGCCATCGAGGTTTCCGGGGCGCAGATCATCACCGTGGCGGTGAGAAGGGTCAACATCT
CGGACCGCAGCAAGGAGTCGCTGCTCGACCATATCGACCTGAAGAAGTACACGCTCCTTCCCAACACCGCCGGCTGCTAC
ACCGCCGAGGACGCCATCCGCACCTGCCGCCTGGCGCGCGAGGCAGGGCTCTCCGATTTCGTGAAACTCGAGGTGCTGGG
CGACGAGAAGACGCTCTATCCCAACAACGAGGAGCTCCTGAAGGCGGCCAAGGTGCTTCTGGCCGAGGGATTCACCGTCC
TTCCCTACACAAGCGACGACCCCATCATCTGCAAAAAGCTGGAGGACATGGGATGCGCCGCGGTGATGCCGCTGGGAGCC
CCCATAGGCTCCGGTCTCGGCATCCGCAACCCCTATAACATCCAGATCATCCTGGAGAGCGTCAAGGTGCCGGTCATCGT
GGACGCGGGGGTCGGGACAGCCTCGGACGCTGCCATCGCCATGGAGCTCGGCTGCCACGGCGTCCTCATGAACACCGCCA
TCGCCGGGGCCAAGGACCCGGTCGCCATGGCAGAGGCGATGAACTGCGCGGTGCGCGCGGGGAGGCTCGCCTACCTGGCC
GGGCGCATCCCGAGGAAGCTCTACGCCAGCGCCTCCTCCCCCTTGGCGGGGCTCATCGGGTGA

Upstream 100 bases:

>100_bases
CCTTCCGGCTTCCCTGCCCAAGGCAGGATAACGAGCGCCCGCCGCACCCGGGCCGGGGTCCCCCGGATCCTTCCGGGAAA
TAGATAATTGGAGAATCGAG

Downstream 100 bases:

>100_bases
AAGAGCTGGAATCCCCCTGGATAGACTTCAACCTCTACCTGATTACCGGCAGGGAGGAGACGCAGGGGCGCCCCCTCGGG
TACGTGGTCGAGGAAGCGCT

Product: thiazole synthase

Products: 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate; 4-hydroxy-benzyl-alcohol; C1 of tyrosine; ThiS protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 260; Mature: 259

Protein sequence:

>260_residues
MTQTNDKLVIAGREFDSRLMVGTGKYADFQQMVRAIEVSGAQIITVAVRRVNISDRSKESLLDHIDLKKYTLLPNTAGCY
TAEDAIRTCRLAREAGLSDFVKLEVLGDEKTLYPNNEELLKAAKVLLAEGFTVLPYTSDDPIICKKLEDMGCAAVMPLGA
PIGSGLGIRNPYNIQIILESVKVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAKDPVAMAEAMNCAVRAGRLAYLA
GRIPRKLYASASSPLAGLIG

Sequences:

>Translated_260_residues
MTQTNDKLVIAGREFDSRLMVGTGKYADFQQMVRAIEVSGAQIITVAVRRVNISDRSKESLLDHIDLKKYTLLPNTAGCY
TAEDAIRTCRLAREAGLSDFVKLEVLGDEKTLYPNNEELLKAAKVLLAEGFTVLPYTSDDPIICKKLEDMGCAAVMPLGA
PIGSGLGIRNPYNIQIILESVKVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAKDPVAMAEAMNCAVRAGRLAYLA
GRIPRKLYASASSPLAGLIG
>Mature_259_residues
TQTNDKLVIAGREFDSRLMVGTGKYADFQQMVRAIEVSGAQIITVAVRRVNISDRSKESLLDHIDLKKYTLLPNTAGCYT
AEDAIRTCRLAREAGLSDFVKLEVLGDEKTLYPNNEELLKAAKVLLAEGFTVLPYTSDDPIICKKLEDMGCAAVMPLGAP
IGSGLGIRNPYNIQIILESVKVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAKDPVAMAEAMNCAVRAGRLAYLAG
RIPRKLYASASSPLAGLIG

Specific function: Catalyzes the rearrangement of 1-deoxy-D-xylulose 5- phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H(2)S

COG id: COG2022

COG function: function code H; Uncharacterized enzyme of thiazole biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thiG family

Homologues:

Organism=Escherichia coli, GI48994993, Length=252, Percent_Identity=49.6031746031746, Blast_Score=240, Evalue=8e-65,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIG_GEOBB (B5ED95)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137477.1
- ProteinModelPortal:   B5ED95
- SMR:   B5ED95
- GeneID:   6783486
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0653
- HOGENOM:   HBG296821
- OMA:   VAIRRTN
- ProtClustDB:   PRK00208
- GO:   GO:0005737
- HAMAP:   MF_00443
- InterPro:   IPR013785
- InterPro:   IPR008867
- Gene3D:   G3DSA:3.20.20.70

Pfam domain/function: PF05690 ThiG; SSF110399 ThiG

EC number: NA

Molecular weight: Translated: 27657; Mature: 27525

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: ACT_SITE 102-102 BINDING 163-163

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQTNDKLVIAGREFDSRLMVGTGKYADFQQMVRAIEVSGAQIITVAVRRVNISDRSKES
CCCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHH
LLDHIDLKKYTLLPNTAGCYTAEDAIRTCRLAREAGLSDFVKLEVLGDEKTLYPNNEELL
HHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCHHHH
KAAKVLLAEGFTVLPYTSDDPIICKKLEDMGCAAVMPLGAPIGSGLGIRNPYNIQIILES
HHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
VKVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAKDPVAMAEAMNCAVRAGRLAYLA
CCCCEEEECCCCCCCCCEEEEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHCHHHHHH
GRIPRKLYASASSPLAGLIG
HHCCHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TQTNDKLVIAGREFDSRLMVGTGKYADFQQMVRAIEVSGAQIITVAVRRVNISDRSKES
CCCCCEEEEEECCCCCEEEEECCCCHHHHHHHHHHHCCCCEEEEEEEEEECCCCCCHHH
LLDHIDLKKYTLLPNTAGCYTAEDAIRTCRLAREAGLSDFVKLEVLGDEKTLYPNNEELL
HHHHCCCCEEEECCCCCCCEEHHHHHHHHHHHHHCCCCCEEEEEEECCCCEECCCCHHHH
KAAKVLLAEGFTVLPYTSDDPIICKKLEDMGCAAVMPLGAPIGSGLGIRNPYNIQIILES
HHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCEEEEEEEC
VKVPVIVDAGVGTASDAAIAMELGCHGVLMNTAIAGAKDPVAMAEAMNCAVRAGRLAYLA
CCCCEEEECCCCCCCCCEEEEECCCCEEEEHHHHCCCCCHHHHHHHHHHHHHHCHHHHHH
GRIPRKLYASASSPLAGLIG
HHCCHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: deoxyxylulose-5-phosphate; ThiS-COSH; L-tyrosine [C]

Specific reaction: deoxyxylulose-5-phosphate + ThiS-COSH + L-tyrosine = 4-methyl-5-(beta-hydroxyethyl)thiazole phosphate + 4-hydroxy-benzyl-alcohol + C1 of tyrosine + ThiS protein [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA