Click here to switch to the map view.

The map label for this gene is hemC

Identifier: 197116806

GI number: 197116806

Start: 483934

End: 484890

Strand: Direct

Name: hemC

Synonym: Gbem_0408

Alternate gene names: 197116806

Gene position: 483934-484890 (Clockwise)

Preceding gene: 197116805

Following gene: 308535161

Centisome position: 10.49

GC content: 63.32

Gene sequence:

>957_bases
ATGGCGCTGAAAGAGCTGAGAATAGGTACCCGCGCGAGCCAGCTCGCACTCTGGCAGGCAAACTGGGTCAAATCCGAGCT
GGAAAAACGCTACCCGGACATGACCGTCACCCTCACCAAGATCAAGACCATCGGGGACAAGATCCTCGACGTGCCCCTGG
CTCAGGTGGGCGGCAAGGGCCTGTTCGTGAAGGAGATCGAGGAGGCGATGCTGCGCGGCGAGATCGACATCGCGGTGCAC
AGCATGAAGGACGTGCCGACCGAATTCCCCGAGGGTCTGGGCCTTTACTGCATCACCGAGCGCGAAGATCCGCGCGATGC
GGTCATCTCCAACGGCGTCAAGTTCGCCGACCTGCCGCAGGGCGCGCGTATCGGCACCTCTGCGCTCAGGCGTCAGGCGC
AGCTCCTCAAGGTGCGTCCCGACCTCGAGATGGTCATCATTCGCGGCAACGTGCAGACACGCATGGACAAGCTTAAGACC
GAAGGGCTTGACGCCGTCATCCTCGCCGCGGCCGGCCTGAACCGCCTGGGCTTTGCCGATCAGATCACCGAGATGCTCCC
GACCGATCTCTCCCTCCCCGCCATCGGCCAGGGCGCGCTGGGCATCGAGTGCAACCTTTCCAACCAGGATGTGAAGGACG
CCATCTCCTTCTTCAACTCTCCGGACACCAGCCGCGCCGTTCGTGCCGAGCGCGCTCTGCTGTGGCGCTGCGAGGGTGGC
TGCCAGGTTCCCATCGCCGCCTTCGGCGAAGTGACCGGCGACCAGCTGAAGCTGACCGGTTTCATCGCCTCCGTCGACGG
CAAGGTTTCCGTCAAGGGAGTCGTGACTGGTCCTGCCGACGACTGCGAGAAGCTTGGTGTGAAGCTCGCCGAGCAGCTCC
TTTCCGAAGGTGGCCACGCCATCCTCGCCGAGGTATACCAGCGTGAAGTCTCCCGGGAGAAGGAAATCCCGGTATAA

Upstream 100 bases:

>100_bases
ATCCAAGGGATTAGGTAGTAGCGGTTAGGGGAACGGCGGATTGTACGCCAGCCCCTAGCCCCTAGTCCCTAATCCCTCGA
TTAACACAAAGGAGAAGTAC

Downstream 100 bases:

>100_bases
CCAAAACCTCAAAAGCACTCACCACAGAGGACACAGAGGTACACAGAGGAAAATCTGAGGAACTACAAAAGCTTTTCGCC
CCCCCCTCCCGCAAGGGGAG

Product: porphobilinogen deaminase

Products: NA

Alternate protein names: PBG; Hydroxymethylbilane synthase; HMBS; Pre-uroporphyrinogen synthase

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MALKELRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLYCITEREDPRDAVISNGVKFADLPQGARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKT
EGLDAVILAAAGLNRLGFADQITEMLPTDLSLPAIGQGALGIECNLSNQDVKDAISFFNSPDTSRAVRAERALLWRCEGG
CQVPIAAFGEVTGDQLKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLLSEGGHAILAEVYQREVSREKEIPV

Sequences:

>Translated_318_residues
MALKELRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVH
SMKDVPTEFPEGLGLYCITEREDPRDAVISNGVKFADLPQGARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKT
EGLDAVILAAAGLNRLGFADQITEMLPTDLSLPAIGQGALGIECNLSNQDVKDAISFFNSPDTSRAVRAERALLWRCEGG
CQVPIAAFGEVTGDQLKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLLSEGGHAILAEVYQREVSREKEIPV
>Mature_317_residues
ALKELRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKGLFVKEIEEAMLRGEIDIAVHS
MKDVPTEFPEGLGLYCITEREDPRDAVISNGVKFADLPQGARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKTE
GLDAVILAAAGLNRLGFADQITEMLPTDLSLPAIGQGALGIECNLSNQDVKDAISFFNSPDTSRAVRAERALLWRCEGGC
QVPIAAFGEVTGDQLKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLLSEGGHAILAEVYQREVSREKEIPV

Specific function: Tetrapolymerization of the monopyrrole PBG into the hydroxymethylbilane pre-uroporphyrinogen in several discrete steps

COG id: COG0181

COG function: function code H; Porphobilinogen deaminase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HMBS family

Homologues:

Organism=Homo sapiens, GI20149500, Length=334, Percent_Identity=38.3233532934132, Blast_Score=224, Evalue=1e-58,
Organism=Homo sapiens, GI66933009, Length=334, Percent_Identity=38.3233532934132, Blast_Score=224, Evalue=1e-58,
Organism=Escherichia coli, GI48994974, Length=303, Percent_Identity=52.1452145214521, Blast_Score=306, Evalue=1e-84,
Organism=Saccharomyces cerevisiae, GI6319996, Length=322, Percent_Identity=36.0248447204969, Blast_Score=187, Evalue=2e-48,
Organism=Drosophila melanogaster, GI20130425, Length=285, Percent_Identity=38.5964912280702, Blast_Score=197, Evalue=6e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HEM3_GEOBB (B5EBG8)

Other databases:

- EMBL:   CP001124
- RefSeq:   YP_002137233.1
- ProteinModelPortal:   B5EBG8
- SMR:   B5EBG8
- GeneID:   6783234
- GenomeReviews:   CP001124_GR
- KEGG:   gbm:Gbem_0408
- HOGENOM:   HBG624842
- OMA:   SLHAVGQ
- ProtClustDB:   PRK00072
- HAMAP:   MF_00260
- InterPro:   IPR000860
- InterPro:   IPR022419
- InterPro:   IPR022417
- InterPro:   IPR022418
- Gene3D:   G3DSA:3.30.160.40
- PANTHER:   PTHR11557
- PIRSF:   PIRSF001438
- PRINTS:   PR00151
- TIGRFAMs:   TIGR00212

Pfam domain/function: PF01379 Porphobil_deam; PF03900 Porphobil_deamC; SSF54782 Porphobil_deam

EC number: =2.5.1.61

Molecular weight: Translated: 34519; Mature: 34388

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS00533 PORPHOBILINOGEN_DEAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALKELRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKG
CCCHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLYCITEREDPRDAVISNGVKFADLPQ
EEHHHHHHHHHCCCHHEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKTEGLDAVILAAAGLNRLGFAD
CCCCCHHHHHHHHHHHEECCCEEEEEEECCHHHHHHHHHHCCCCEEEEEHHCCCCCCCHH
QITEMLPTDLSLPAIGQGALGIECNLSNQDVKDAISFFNSPDTSRAVRAERALLWRCEGG
HHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHEEEEECCC
CQVPIAAFGEVTGDQLKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLLSEGGHA
CCCCHHHHCCCCCCEEEEEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCHH
ILAEVYQREVSREKEIPV
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
ALKELRIGTRASQLALWQANWVKSELEKRYPDMTVTLTKIKTIGDKILDVPLAQVGGKG
CCHHHCCCCCHHHEEEHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHCCHHHHCCCC
LFVKEIEEAMLRGEIDIAVHSMKDVPTEFPEGLGLYCITEREDPRDAVISNGVKFADLPQ
EEHHHHHHHHHCCCHHEEEECHHCCCCCCCCCCCEEEEECCCCCHHHHHHCCCEEECCCC
GARIGTSALRRQAQLLKVRPDLEMVIIRGNVQTRMDKLKTEGLDAVILAAAGLNRLGFAD
CCCCCHHHHHHHHHHHEECCCEEEEEEECCHHHHHHHHHHCCCCEEEEEHHCCCCCCCHH
QITEMLPTDLSLPAIGQGALGIECNLSNQDVKDAISFFNSPDTSRAVRAERALLWRCEGG
HHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHHHHHHHCCCCHHHHHHHHHHEEEEECCC
CQVPIAAFGEVTGDQLKLTGFIASVDGKVSVKGVVTGPADDCEKLGVKLAEQLLSEGGHA
CCCCHHHHCCCCCCEEEEEEEEEECCCEEEEEEEEECCHHHHHHHHHHHHHHHHHCCCHH
ILAEVYQREVSREKEIPV
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA