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The map label for this gene is hemD [H]

Identifier: 308535161

GI number: 308535161

Start: 485161

End: 486705

Strand: Direct

Name: hemD [H]

Synonym: NA

Alternate gene names: 308535161

Gene position: 485161-486705 (Clockwise)

Preceding gene: 197116806

Following gene: 197116808

Centisome position: 10.51

GC content: 64.01

Gene sequence:

>1545_bases
ATGAGCGCTGATATGAAGAACGGATATGTCTATCTGATCGGTGCGGGTCCTGGAGACCCGGGCCTGATCACGGTGAAAGG
GCGCGATTGCCTGACCAAGGCCCAGGTGGTGATGTACGACTACCTGGCCAACGAGGAACTGCTGCGCCTCGCCCCGAAGG
GGGCGGAGCTCATCTACGCCGGCAAGGTCGGCGGCGAGCACAACCGGGAGCAGAGCCAGATCAACGAACTACTGGTACAG
AAGGCGCTCTCGGGCAAGATCGTGGCGAGACTCAAGGGGGGCGATTCCTTCATCTTCGGGCGCGGCGGCGAAGAATGCGA
GGCGCTGGTCGAGGCCGGCATCCCCTTCGAGATCGTCCCCGGCATCACCGCTGCAGTCGGCGCCACCAGCTATGCCGGTA
TCCCCCTTACCCATCGCGGCGTGACCACCTCCGTCGCCTTCGTCACCGGGCACGAGAAGAAAGGGAAGGCCAGTTCCGAA
ATCGATTGGGAAGGTCTCTCTCTGGGAAGCGGCACCGTCGTCTTCTACATGGGCGTCACCAACCTGCCGCACATAGCCTG
CAGCATGATGGAGCACGGCCGCGCGCCGCAGACCCCGGTGGCGCTGATCCGCTGGGGCACCCGCCCCGAGCAGGAAGTGC
TGGTCGGAACGCTGGCGAACATCGCAGAAAAGGCGAAGGCCGCCGAGTTCAAGGCGCCCGCCATCACCGTAGTCGGCGAA
GTGGTCAACCTCAGGGAAACCCTGCGCTGGTTCGACAACCGCCCGCTCTTCGGGCGCTCGGTCCTGGTGACCCGCGGCGC
CGACCAGGCGGGCGGGTTCACCTCCAAGCTGGAGCAGCTCGGCGCCAGGGCTTACTGCTGCCCGACCATCGAAATCACCC
CGCCCACCGATCATTCCGCACTCGACGAGGCGATCGAGGGGCTGGACAGCTTCAACTGGATCATCTTCACCTCGTACAAC
GCCGTGAAGTACTTCTTCGCCAGACTCTCCGAGCACAACCTCGACACCCGTGCCCTCGGCCGCTGCCGCGTCTGCGCCGT
CGGTCCCAAGACCGCCGCGGCGCTGGCTCAATACGGCATTCGGGCCGATCTGATCCCTTCCGATTACAAGGCCGAGGGGG
TCATCGAAATCTTTTCCGAGATGGACATGGCAGGACAATGGGTGCTTTTCCCCAAAGGCGACCGTGCCCGCGAAGTCATC
ACCGAGGAGTTGGGGCGGATGGGAGCCCAGGTCATCGCACCTGTCGCCTATGCGAACCACACCCCTGACGATATCCCAGC
CGAGGCGCTCGCCGCCCTCGAGGACGGTCGCATCGACTGCGCCACCTTCACCTCCTCCTCCACCGTGCAGAACTTGGCCG
CCATCCTGGGCGAGAACCGGTTCCTGCATCTGATGCAAGGAGTAAAGGTCGCCTCCATCGGCCCCATCACCTCCAAGACC
TGCCGGGATCTGGGGCTTGAGGTGCACATGGAGCCCGCCGAGTACACGCTCGACGCGCTGGCCGCGGAGATGATCGAATA
TTTTCGCGCAGGGGAATTACGGTAA

Upstream 100 bases:
NA

Downstream 100 bases:
NA

Product: bifunctional uroporphyrinogen III C2,C7-methyltransferase/uroporphyrinogen III synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Uroporphyrinogen-III synthase; UROS; Hydroxymethylbilane hydrolyase [cyclizing]; Uroporphyrinogen-III cosynthase [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:
NA

Sequences:

>Translated_514_residues
MSADMKNGYVYLIGAGPGDPGLITVKGRDCLTKAQVVMYDYLANEELLRLAPKGAELIYAGKVGGEHNREQSQINELLVQ
KALSGKIVARLKGGDSFIFGRGGEECEALVEAGIPFEIVPGITAAVGATSYAGIPLTHRGVTTSVAFVTGHEKKGKASSE
IDWEGLSLGSGTVVFYMGVTNLPHIACSMMEHGRAPQTPVALIRWGTRPEQEVLVGTLANIAEKAKAAEFKAPAITVVGE
VVNLRETLRWFDNRPLFGRSVLVTRGADQAGGFTSKLEQLGARAYCCPTIEITPPTDHSALDEAIEGLDSFNWIIFTSYN
AVKYFFARLSEHNLDTRALGRCRVCAVGPKTAAALAQYGIRADLIPSDYKAEGVIEIFSEMDMAGQWVLFPKGDRAREVI
TEELGRMGAQVIAPVAYANHTPDDIPAEALAALEDGRIDCATFTSSSTVQNLAAILGENRFLHLMQGVKVASIGPITSKT
CRDLGLEVHMEPAEYTLDALAAEMIEYFRAGELR
>Mature_513_residues
SADMKNGYVYLIGAGPGDPGLITVKGRDCLTKAQVVMYDYLANEELLRLAPKGAELIYAGKVGGEHNREQSQINELLVQK
ALSGKIVARLKGGDSFIFGRGGEECEALVEAGIPFEIVPGITAAVGATSYAGIPLTHRGVTTSVAFVTGHEKKGKASSEI
DWEGLSLGSGTVVFYMGVTNLPHIACSMMEHGRAPQTPVALIRWGTRPEQEVLVGTLANIAEKAKAAEFKAPAITVVGEV
VNLRETLRWFDNRPLFGRSVLVTRGADQAGGFTSKLEQLGARAYCCPTIEITPPTDHSALDEAIEGLDSFNWIIFTSYNA
VKYFFARLSEHNLDTRALGRCRVCAVGPKTAAALAQYGIRADLIPSDYKAEGVIEIFSEMDMAGQWVLFPKGDRAREVIT
EELGRMGAQVIAPVAYANHTPDDIPAEALAALEDGRIDCATFTSSSTVQNLAAILGENRFLHLMQGVKVASIGPITSKTC
RDLGLEVHMEPAEYTLDALAAEMIEYFRAGELR

Specific function: May catalyze sequential reactions to synthesize uroporphyrinogen III from hydroxymethylbilane (HMB) and then precorrin-2, which are intermediate compounds in both vitamin B12 and siroheme biosyntheses [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=246, Percent_Identity=47.1544715447154, Blast_Score=234, Evalue=9e-63,
Organism=Saccharomyces cerevisiae, GI6322922, Length=238, Percent_Identity=29.8319327731092, Blast_Score=111, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR003754
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR003043 [H]

Pfam domain/function: PF02602 HEM4; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =4.2.1.75 [H]

Molecular weight: Translated: 55343; Mature: 55212

Theoretical pI: Translated: 5.16; Mature: 5.16

Prosite motif: PS00839 SUMT_1 ; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSADMKNGYVYLIGAGPGDPGLITVKGRDCLTKAQVVMYDYLANEELLRLAPKGAELIYA
CCCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEE
GKVGGEHNREQSQINELLVQKALSGKIVARLKGGDSFIFGRGGEECEALVEAGIPFEIVP
ECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECC
GITAAVGATSYAGIPLTHRGVTTSVAFVTGHEKKGKASSEIDWEGLSLGSGTVVFYMGVT
CCHHHHCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEECCC
NLPHIACSMMEHGRAPQTPVALIRWGTRPEQEVLVGTLANIAEKAKAAEFKAPAITVVGE
CCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VVNLRETLRWFDNRPLFGRSVLVTRGADQAGGFTSKLEQLGARAYCCPTIEITPPTDHSA
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCEEEECCCCCHHH
LDEAIEGLDSFNWIIFTSYNAVKYFFARLSEHNLDTRALGRCRVCAVGPKTAAALAQYGI
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCCCEEEEEECCHHHHHHHHCCC
RADLIPSDYKAEGVIEIFSEMDMAGQWVLFPKGDRAREVITEELGRMGAQVIAPVAYANH
EECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCC
TPDDIPAEALAALEDGRIDCATFTSSSTVQNLAAILGENRFLHLMQGVKVASIGPITSKT
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHEEHHCCCEEEECCCCCHHH
CRDLGLEVHMEPAEYTLDALAAEMIEYFRAGELR
HHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SADMKNGYVYLIGAGPGDPGLITVKGRDCLTKAQVVMYDYLANEELLRLAPKGAELIYA
CCCCCCCEEEEEECCCCCCCEEEECCCCHHHHHHHHHHHHHCCCHHHHCCCCCCCEEEE
GKVGGEHNREQSQINELLVQKALSGKIVARLKGGDSFIFGRGGEECEALVEAGIPFEIVP
ECCCCCCCHHHHHHHHHHHHHHHCCEEEEEEECCCEEEECCCCHHHHHHHHCCCCEEECC
GITAAVGATSYAGIPLTHRGVTTSVAFVTGHEKKGKASSEIDWEGLSLGSGTVVFYMGVT
CCHHHHCCCCCCCCCEECCCCEEEEEEEECCCCCCCCCCCCCCCCEECCCCCEEEEECCC
NLPHIACSMMEHGRAPQTPVALIRWGTRPEQEVLVGTLANIAEKAKAAEFKAPAITVVGE
CCHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
VVNLRETLRWFDNRPLFGRSVLVTRGADQAGGFTSKLEQLGARAYCCPTIEITPPTDHSA
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCHHHHHHHHCCCEEECCEEEECCCCCHHH
LDEAIEGLDSFNWIIFTSYNAVKYFFARLSEHNLDTRALGRCRVCAVGPKTAAALAQYGI
HHHHHHCCCCCCEEEEECCHHHHHHHHHHHHCCCCHHHCCCEEEEEECCHHHHHHHHCCC
RADLIPSDYKAEGVIEIFSEMDMAGQWVLFPKGDRAREVITEELGRMGAQVIAPVAYANH
EECCCCCCCCCCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHHCCHHHHHHHHHCCC
TPDDIPAEALAALEDGRIDCATFTSSSTVQNLAAILGENRFLHLMQGVKVASIGPITSKT
CCCCCCHHHHHHHHCCCEEEEEECCCHHHHHHHHHHCCCHHEEHHCCCEEEECCCCCHHH
CRDLGLEVHMEPAEYTLDALAAEMIEYFRAGELR
HHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7665501 [H]